JPH11335245A - Hair growing and restoring agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare a hair growing and restoring agent containing water extract from Nardostachy jatamansi D.C. as an active principle and activating the action of hair mother cells in hair roots, etc., through insulinoid action to grow and restore hair. SOLUTION: This hair growing and restoring agent contains water extract from Nardostachy jatamansi D.C. as an active principle. The water extract is prepared by directly putting water over a fire, wherein the water is made to contact as quickly as possible with chopped Nardostachy jatamansi D.C., and by distillation. The formulation amount of the water extract in this agent is pref. 1.0×10<-7> -1.0×10<-3> wt.%. The formulation can be solution, emulsion, cream, aerosol, etc.,, and the type of its formulation can be tonic, hair cream, mousse, etc. This agent can be formulated, if needed, with an oleaginous ingredient (e.g. a higher fatty acid), an aqueous ingredient, a humectant (e.g. glycerin), a thickener (e.g. quince mucilage), a vasodilator (e.g. nicotinic acid amide), amino acids (e.g. serine), etc.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a hair restorer in the technical field. More specifically, the present invention relates to a hair-growth agent that grows hair by activating the activity of hair matrix cells of hair roots by an insulin-like action.

[0002]

2. Description of the Related Art In a modern society referred to as an aging society or a stressed society, there are more and more opportunities for head hair to be exposed to the risk of hair loss due to various causes. In response to this, various attempts have been made to provide a more excellent “hair restorer”. However, in spite of such attempts, conventional hair restorers do not prevent hair loss,
Hair growth effects such as a hair growth effect were not always sufficient. This is probably because hair loss is caused by various factors and the mechanism of hair growth is very complicated. The hair restoration agents provided so far have been developed by taking into account the relatively rough concept of hair loss, in other words, simply the phenomenon of "hair loss", and few have developed into the mechanism.

[0003] If a hair restorer whose action point is clarified is developed, it is possible to prevent hair loss and the like by selecting or combining the hair restorer according to the symptoms and the like in a more clear form. , More effective hair care can be performed. Therefore, there is a need in the industry to find a component having a more clear and characteristic hair-growth action and to provide a hair-growth agent containing such a component as an active ingredient.

[0004]

Recently, an epoch-making fact has been revealed regarding this hair growth effect. That is,
It has been reported that those with elevated insulin-like growth factor-I (IGF-I) in the blood may have hirsutism, and this IGF-I exerts some effects on hair growth. [Freinkel N: Implic
ations of diabetes in pregrancy for developmental
biology. Verhandlungen derdeutshen gesellschaft fu
r innere medizin 93: p266-274 (1987 )].

[0005] Furthermore, regarding the hair-growth effect of IGF-I, it has been observed in insulin as well as IGF-I in a human organ culture system [Pilpott MP, Sanders D.
A, Kealey T: Effects of insulin and insulin-like gro
wth factors on cultured human hair follicles: IGF-I
at physoliogic concentrations is an important reg
ulator of hair follicle growth in vitro .The journal
l of investigative dermatology 102: p857-861 (1994) ]
The growth of androgen-dependent outer root sheath cells in human papillary hair follicle-derived hair papilla and outer root sheath cell culture was determined by IGF-I
Antibody inhibition [Itami S, Kurata S, Sonoda T, Ta
kayasu S: Interaction between dermal papilla and fo
llicular epithelial cells in vitro: effect of anaro
gen . : British journal of dermatology 132: p527-532 (1
995) ], during the regression phase of the rat hair cycle,
Decrease in IGF-I mRNA levels [Little J
C, Westgate GE, Erans A, Granger SP: Cytokine gene exp
ression in intact anagen rat hair follicles .The jo
urnal of investigative dermatology 103: p715-720 (19
94) ],

[0006] The fact that the IGF-I receptor is specifically expressed in hairy cells undergoing active cell division [Hodak E,
Gottlieb AB, Anzilotti M, Krueger JG: the insulin-
likegrowth factor 1 receptor is expressed by epith
emal cells with proliferative potential in human e
pidermis and skin appendages: correlation of incre
ased expression with epidemal hyperplasia.The jou
rnal of investigati ve dermatology 106: p564-570 (199
6)] and that IGF-I is expressed in human dermal papilla cells [Tavakkol A, Elder JT, Griffiths CEM, Co.
oper KD, Talwar H, Fisher GJ, Keane KM, Foltin SK,
Voorheas JJ: Expression of growth hormone recepto
r, insulin-like growth factor 1 (IGF-1) and IGF-1 r
eceptormRNA and proteins in human skin.The journa
l of investigative dermatology 99: p343-349 (1992)]
Etc. are clear.

[0007] That is, IGF-I or insulin activates the action of the hair root consisting of the dermal papilla,
It was revealed that a hair growth effect was observed.
A hair restorer containing FI as an active ingredient is already known (Japanese Patent Publication No. 4-60567). However, IGF
Neither -I nor insulin is a component derived from animals and has a large molecular weight, so that problems such as difficulty in transdermal absorption by external application are recognized.

Therefore, the problem to be solved by the present invention is that
By finding a plant-derived component having the same action as GF-I and insulin, and using this as an active ingredient, the point of action is provided, and by providing a characteristic hair restorer, the demand in the above-mentioned industry is reduced. It is to provide a way to meet.

[0009]

Means for Solving the Problems The inventor of the present invention has made intensive studies for solving the problems. As a result, it was found that a desired insulin-like activity was observed in the water extract of Ganmatsu (Nardostachy jatamansi DC root and / or rhizome) belonging to the pine stalk family, and the water extract of Ganmatsu was regarded as an active ingredient. The present inventors have found that this problem can be solved by providing a hair restorer that does this, and have completed the present invention.

[0010] That is, the present invention is an invention which provides a hair restorer containing an extract of sweet pine with water as an active ingredient.

In the present invention, "extraction with water"
As long as the substance that mediates the extraction of sweet pine is water (H ₂ O), the extraction form is not limited, and the extraction by immersing the sweet pine in ordinary water, as well as the extraction with hot water And extraction by using steam.

[0012]

Embodiments of the present invention will be described below. The sweet pine, which is a raw material of the water extract and is used as an active ingredient of the hair restorer according to the present invention (hereinafter referred to as the hair restorer of the present invention), is Nardostachys yamatanshii (Nar
dostachy jatamansi DC) means the root and / or rhizome.

[0013] Nardostakis Yamatanshii 4-8
cm, a perennial plant that grows naturally in the Himalayas, and the essential oil obtained from its roots and / or rhizomes, Amatsu, is used as an aromatic stomach medicine and fumigant as a raw material for perfumes as "Amatsumatsu". ing.

[0014] As described above, the extract of Ganmatsu by water used in the present invention does not matter in its extraction form as long as the substance that mediates the extraction of Ganmatsu is water (H ₂ O). .

For example, a water extract of kanmatsu can be obtained by heating and distilling the water over an open flame while contacting the finely cut kanmatsu with water as quickly as possible.

Further, by exposing the sweet pine in such a manner that the steam is brought into a saturated state and is not brought into direct contact with water, an aqueous extract of sweet pine can be obtained.

Further, by exposing the sweet pine to saturated or superheated steam intensely and often at high pressure,
An aqueous extract of sweet pine can be obtained.

This "extraction with water" refers to another extraction method using an organic solvent other than water, for example, "extraction method using a water-soluble organic solvent alone or a water-containing organic solvent" (Japanese Patent Laid-Open No.
And JP-A-4-38014) in that no organic solvent other than water such as primary alcohol is used at all.

The amount of the water extract of Kanmatsu obtained as described above in the hair restorer of the present invention is 1.0 × 10 ^{−7 to} 10.0% by weight, preferably 1.10% by weight, based on the whole hair restorer. 0 × 10 ^{−7 to} 1.0 × 10 ⁻³ % by weight. If the amount is less than 1.0 × 10 ⁻⁷ % by weight based on the whole hair restorer, the desired hair-growth action based on the insulin-like action is not sufficiently exhibited, which is not preferable. When combined, it comes with a strong smell peculiar to Kanmatsu,
Not preferred.

Thus, the hair restorer of the present invention containing the water extract of sweet pine as an active ingredient brings an insulin-like action to the head hair while being a plant ingredient,
Thereby, an excellent hair growth effect can be exhibited.

The method for evaluating whether or not the compound has an insulin-like effect is known in the art, for example, human breast cancer cells M whose growth depends on insulin.
An evaluation method using CF-7 (specifically, described in Examples described later) and the like can be used.

The dosage form that the hair growth agent of the present invention can take is not particularly limited as long as it is a dosage form applicable to the outer skin of the head. For example, a dosage form such as a liquid, an emulsion, a cream, or an aerosol is selected. be able to. The form of the hair restorer of the present invention is also optional, and for example, it can take the form of tonic, hair cream, mousse, shampoo, rinse, milky lotion, lotion, pack, aerosol and the like.

In the hair restorer of the present invention, cosmetics, quasi-drugs, and pharmaceuticals, in addition to the essential component of the aqueous extract of sweet pine, if necessary and as long as the desired effects of the present invention are not impaired. Commonly used in, for example, various oily or aqueous components, humectants, thickeners, preservatives, antioxidants, fragrances,
Coloring agents, various chemicals and the like can be blended.

For example, higher fatty acids, solid paraffin, liquid paraffin, silicone oil, squalane, glyceryl monooleate, olive oil, isopropyl myristate, higher alcohols and other oils; glycerin, hyaluronic acid, propylene glycol, maltitol, atelocollagen, lactic acid Humectants such as sodium; quince,
Thickeners such as carboxyvinyl polymer and xanthan gum; nicotinamide, benzyl nicotinate, vitamin E acetate, assembly extract, carpronium chloride,
Vasodilators such as acetylcholine derivatives; amino acids such as serine, methionine, and arginine; vitamins such as vitamin B ₆ , vitamin E, biotin, pantothenic acid;
Nicotinic acid esters such as nicotinic acid, methyl nicotinate, and tocopherol nicotinate; skin function enhancers such as cepharanthin; female hormones such as estradiol;
Anti-inflammatory agents such as glycyrrhizic acid, glycyrrhetinic acid and azulene; antibacterial agents such as hinokitiol; cooling agents such as menthol; salicylic acids, zincs, lactic acids and the like; and organic acids such as citric acid can be added.

In particular, by adding a known hair-growth component, which is known to have a hair-growth effect by an action other than the insulin-like action, a more effective hair-growth effect can be expected in the hair-growth agent of the present invention. The specific formulation of the hair restorer of the present invention will be described later.

[0026]

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to Examples, but it should not be construed that the technical scope of the present invention is limited by these Examples. In addition, in the following Examples and the like, the numerical value representing the blending amount is represented by% by weight with respect to the entire target to be blended unless otherwise specified.

Preparation of Sample 1.65 kg of sweet pine was steamed in a kettle, and the steam was recovered and cooled, so that the water extract of sweet pine was converted into a distillate by 4 kg.
0.56 g was obtained. This sample was used as an aqueous extract of sweet pine,
Used in the following examples.

Examination of Insulin-Like Activity of Samples Whether or not an insulin effect directly related to the hair-growth effect was observed in a sample was determined by the human breast cancer cell MCF-7, which has already been established as an assay for insulin-like activity. (Hereinafter also referred to as MCF-7 cells). This assay method is an assay method for insulin-like activity utilizing the fact that MCF-7 cells are greatly affected by the presence or absence or concentration of insulin or IGF-I.

Hereinafter, this test system will be specifically described. Preparation of pre-cultured MCF-7 cells: MCF-7 cells (AT
CC No. : HTB 22) was added to a phenol red-containing DF medium containing 7.5% FBS and 44 mM NaHCO ₃ (manufactured by Gibco, DMEM: Ham F-1).
2 = 1: 1, hereinafter also referred to as a maintenance medium).
Cultivation was carried out under the conditions of ° C. and 7.5% CO ₂ . To maintain MCF-7 cells in logarithmic phase, they were subcultured twice a week with 0.05% trypsin / PBS and 0.02% EDTA / PBS (1.5 ×). 10 ⁴ ce
passaged in ll / cm ^2). Then, in order to prepare cells for bioassay, 1.5 × 10 ⁴ cell
/ cm ² , and cultured under the same conditions as above (37 ° C., 7.5% CO ₂ ) for 4 days.
CF-7 cells were obtained.

Day 1 of assay: The precultured MCF-7 cells were treated with trypsin, peeled from the culture plate, the trypsin reaction was stopped with a maintenance medium, and centrifuged to collect the MCF-7 cells. Then, an adhesion medium [Phenol Red was removed from the maintenance medium and 30 mM Na
₂ SeO ₃ , 10 μg transferrin (SIGMA
(T-2158), 0.2% BSA and 5% DCC
(Dextran coated activated carbon treatment) -medium supplemented with FBS], carefully pipetting, and then filtering the cells with a 40 μm cell strainer to remove cell clumps. Then, the suspension of MCF-7 cells was placed in a 24-well plate for cell culture at 1.5 × 1.
0 ⁴ performs plated at a cell density of cell / well / cm ^2, and cultured in the above and similar conditions _{(37 ℃ · 7.5% CO 2} ).

Assay 2nd day: The culture medium was prepared by removing the DCC-FBS from the above-mentioned adhesion medium,
The medium was replaced with the removal medium, and then the same conditions as above (at 37 ° C.)
Culture was performed at 7.5% CO ₂ ).

Assay day 3: Into the FBS-free medium (1 mL) used on the second day of the assay, the water extract of Kanmatsu was added at a predetermined concentration [based on the dry weight of the water extract of Kanmatsu.
0 (negative control), 0.0001 ppb,
0.01ppb, 1ppb, 0.1ppm, 1ppm and 10pp
m) was replaced with the culture medium added so that
Subsequently, the cells were cultured under the same conditions as described above (37 ° C., 7.5% CO ₂ ).

Assay day 7: M cultured in PBS
The CF-7 cells were gently washed twice and the cells were each stored at -20 ° C. Then, 2 μg / mL Hoechst
Phosphate buffer containing 33258 (40 mM Na ₂ HPO ₄ ,
10 mM NaH ₂ PO ₄ , 2.0 M NaCl, 2 mM
EDTA, pH 7.4) was added to each well in an amount of 1 mL, and sonication was performed to suspend MCF-7 cells in the wells.
Performed for 0 seconds. The MCF-7 cells thus obtained were placed in a cuvette, and the fluorescence intensity was measured (excitation light 356).
nm, emission 458 nm).

Using bovine thymus DNA as a standard, the amount of DNA per well was determined by the method of Labarca and Paigen (198).
0, Anal. Biochem., 102, 344). The result is shown in FIG. According to FIG. 1, the amount of DNA significantly increased in the system to which the aqueous extract of Kanmatsu was added at 0.1 ppm and 1 ppm in terms of dry weight as compared with the control group, and insulin-like activity closely related to the hair growth effect was observed. It was found that activity was observed. From this fact, it was clarified that the water extract of Kanmatsu had excellent hair-growth activity.

Hereinafter, examples of the formulation of the hair restorer of the present invention will be shown as examples. All of the hair restorers of these formulas showed clear insulin-like activity. [Example 1] Creamy hair restorer compounding ingredients (% by weight) (A phase) Liquid paraffin 5.0 Cetostearyl alcohol 5.5 Glyceryl monostearate 3.0 EO (20 mol) -2-octyldodecyl ether 3.0 Propylparaben 0.3 Perfume 0.1 (Phase B) Amamatsu water extract 0.01 Glycerin 8.0 Dipropylene glycol 20.0 Polyethylene glycol 4000 5.0 Sodium dodecyl sulfate 0.1 Sodium hexametaphosphate 0.0005 Deionized water remaining

<Preparation Method> The phases A and B were each dissolved by heating, mixed and emulsified by a homomixer to obtain a creamy hair restorer.

Example 2 Hair restorer compounding ingredients compounding amount (% by weight) Amamatsu water extract 0.0004 Stearyl dimethylamine oxide 0.4 Hardened castor oil ethylene oxide (40 mol) adduct 1.0 95% ethanol 54.0 Remaining ion-exchanged water

<Production method> Ion-exchanged water was added to 95% ethanol, hydrogenated castor oil ethylene oxide (40 mol) adduct and stearyl dimethylamine oxide were added, and an aqueous extract of Kanmatsu was added, followed by stirring. A hair restorer was obtained.

Example 3 Hair restorer compounding ingredients compounding amount (% by weight) Amamatsu water extract 0.008 polyoxyethylene laurylamine oxide 0.1 dimethyllaurylamine oxide 1.0 hydrogenated castor oil ethylene oxide (40 mol) ) Adduct 1.0 95% ethanol 54.0 Perfume Appropriate amount Deionized water remaining amount

<Production method> Ion-exchanged water was added to 95% ethanol, hydrogenated castor oil ethylene oxide (40 mol) adduct, polyoxyethylene laurylamine oxide, and dimethyllaurylamine oxide were added. The product was added and stirred to obtain a hair restorer.

[0041]

Industrial Applicability According to the present invention, there is provided a hair restorer capable of exhibiting an excellent hair restorer effect from the aspect of insulin-like activity.

[Brief description of the drawings]

FIG. 1 is a drawing showing the results of examining the effect of the addition of a water extract of Kanmatsu on DNA synthesis in MCF-7 cells.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Tsutomu Soma 2-12-1 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa Prefecture Inside Shiseido 2nd Research Center Co., Ltd. (72) Inventor Jun Suzuki 2 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa -12-1 In Shiseido Second Research Center Co., Ltd. (72) Inventor Masahiro Tajima 1050 Nippa-cho, Kohoku-ku, Yokohama, Kanagawa Prefecture In Shiseido First Research Center Co., Ltd. (72) Inventor Tomomi Okazaki Yokohama, Kanagawa Prefecture 1050 Nippa-cho, Kohoku-ku, Shiseido Daiichi Research Center

Claims (1)

[Claims] 1. A hair restorer comprising, as an active ingredient, an extract of water of Kanmatsu (Nardostachy jatamansi DC root and / or rhizome).