JPH1132574A - Tool for forming inoculation hole of culture medium for cultivating mushroom - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a tool for forming an inoculation hole capable of surely forming a space for inoculating a spawn in an opening of a container by forming the inoculation hole so as to retain the shape thereof even after carrying out the autoclave sterilizing treatment and adjusting the shape of the culture medium near the opening of the container. SOLUTION: This tool for forming an inoculation hole of a culture medium for cultivating mushrooms is obtained by forming a rod-like core 3 for forming the inoculation hole of a heat-resistant material such as polypropylene resin, integrally installing a disk-like flange 4 at one end of the rodlike core 3, integrally arranging a connecting shaft 5 protruding from the center of the end face of the flange 4 in the direction coaxial with the rodlike core 3 and freely detachably providing a lid (cap) 6 capable of closing an opening of a container at the tip of the connecting shaft 5. In the culture medium for cultivating the mushrooms, the rod-like core 3 is inserted into the inoculation hole preformed by a usual method and the autoclave sterilization is directly carried out to thereby thermoform the inoculation hole. The inoculation hole is surely maintained even by taking out the rod-like core 3 after cooling the culture medium.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a tool for forming an inoculation hole for a culture medium for mushroom cultivation.

[0002]

2. Description of the Related Art In recent years, as a method of cultivating edible mushroom fungi, a fungal bed cultivation method which enables shortening of the cultivation period and year-round cultivation instead of the conventional log cultivation method has been widely adopted.

[0003] In the fungal bed cultivation method, a granular material such as sawdust is used as a main material of a culture medium, and a secondary material containing a relatively large amount of nutrients such as rice bran or bran is mixed with the medium.
This is a cultivation method using a medium material adjusted to about 65%. In this method, the medium material is contained in a container such as a bottle or bag made of a heat-resistant synthetic resin. The container is contained in a sterilization pot and sterilized by heating (hereinafter, referred to as autoclave sterilization) and then sterilized. In the room, the inoculum of the mushroom fungus is inoculated, and then the bed is matured so as to generate fruiting bodies (mushrooms).

[0004] The autoclave sterilization described above is performed in order to prevent the propagation of various bacteria in the medium. In the so-called high-pressure sterilization method, the inside of the medium is heated at about 120 ° C under a pressure of about 1.2 kg / cm ^2. Heat for ~ 2 hours and then degas. In the normal-pressure sterilization method, the medium temperature is usually maintained in a sterilization pot at about 95 to 100 ° C. for 6 to 10 hours.

[0005] Such a container containing a culture medium for mushroom cultivation, which is essential for cultivation of a fungus bed, is made of a heat-resistant material (heat-resistant synthetic resin, heat-resistant glass, etc.) that does not deform or change in quality while withstanding autoclave sterilization, and has a wide-mouth bottle shape. Bottle-shaped ones are often used.

Before the medium is sterilized in an autoclave, a rod that is rotated by a rotating power such as a motor is forcibly inserted into a required portion of the medium by a conventional method to have a diameter of about 1.
Form an inoculation hole with a depth reaching the bottom of the container at about 5 cm,
Perform autoclave sterilization with the rod removed,
After cooling, the inoculum is inoculated.

[0007] The form of the inoculum is in the form of powder and granules obtained by inoculating a mushroom fungus on the surface of a medium material such as sawdust, cereal grains and the like and culturing it for a predetermined period of time. In addition, the inoculum is also sprayed on the area around the opening of the inoculation hole of the medium (the surface of the medium).

[0008]

As described above, in the step of inoculating the inoculum, it is essential that an inoculation hole of an appropriate size is formed in the medium in the container after the autoclave sterilization. In the medium using hardwood sawdust and nutrients such as rice bran and bran, the shape of the inoculum formed before the autoclave is easily maintained even after autoclaving.

However, the shape of the inoculation hole formed before heat sterilization may not be maintained until after sterilization due to the difference in the material of the culture medium (the type of the material plant and the portion used).

[0010] For example, if a highly elastically deformable material such as corn germ meal (corn oil pomace) is used as a nutrient together with sawdust, the inoculation hole previously formed before heat sterilization is likely to be closed at that time. ,
Furthermore, there is a problem in that deformation due to heat sterilization easily causes blockage, distortion, or reduced deformation.

In addition, at the opening (particularly, the opening of a wide-mouthed bottle) of the container containing the culture medium for mushroom cultivation, the medium expands and swells due to heating during autoclave sterilization, and the medium overflows from the container opening to the outside. In some cases, the culture medium came into contact with the lower surface of the lid for sealing the opening, and there was no space for inoculating the inoculum.

At first glance, it is also conceivable that it is possible to maintain the shape and size of the inoculation hole by adopting a method such as forming an inoculation hole in the medium after autoclaving and sterilizing the medium near the container opening. However, it is practically difficult to carry out the culture while maintaining the sterility of the culture medium. Therefore, in practice, it was not possible to form the inoculation hole and maintain the shape while maintaining the sterilization state of the culture medium.

Therefore, an object of the present invention is to solve the above-mentioned problems and to maintain the shape of the inoculation hole (the shape of the opening and the inside of the hole) even after the autoclave sterilization treatment. The purpose of the present invention is to adjust the shape of the surface so as to secure a space capable of inoculating a seed bacterium in the opening of the culture vessel.

[0014]

In order to solve the above-mentioned problems, according to the present invention, a rod-shaped core is provided which can be inserted into an inoculation hole of a culture medium for mushroom cultivation. The inoculation hole forming tool for the culture medium for mushroom cultivation was provided with a flange for forming the plate into a flat shape.

Further, a lid capable of closing the opening of the container containing the mushroom cultivation medium is provided at one end of the rod-shaped core of the tool for forming an inoculation hole for the mushroom cultivation medium. It is preferable that the above-mentioned mushroom fungus inoculation aid has a lid detachably provided on the rod-shaped core.

The tool for forming an inoculation hole for a mushroom fungus according to the present invention is obtained by inserting a rod-shaped core into an inoculation hole formed in a mushroom cultivation medium in advance by a usual method and then autoclaving the mushroom cultivation medium. The inoculation hole can be formed by heating. Then, cool the medium (normally cool)
By subsequently extracting the rod-shaped core, a sterilized medium in which the required inoculation hole is reliably formed in the container opening can be obtained.

The periphery of the opening of the inoculation hole on the surface of the sterilized medium is sterilized in an autoclave while being pressed flat by the flange of the rod-shaped core.
The periphery of the opening of the inoculation hole is formed into a flat shape by heating. For this reason, the heat expansion near the opening of the inoculation hole on the surface of the culture medium is smaller than that in the autoclave sterilization medium in which a rotating rod is inserted into the center of the culture medium to form an inoculation hole and the inoculation hole is not formed as before. Instead, a sterilized medium in which a space capable of inoculating the inoculum is reliably left immediately below the opening of the container can be obtained.

[0018]

BEST MODE FOR CARRYING OUT THE INVENTION The mushroom fungus to be cultivated in the present invention is not limited as long as it can be effectively used in the food industry, the pharmaceutical industry and other industries, and its use and types are not particularly limited. For example, edible mushrooms such as shiitake, bunashimeji, enokitake, nameko, oyster mushroom, and maitake are typical examples.

The medium used in the present invention can be used without any particular limitation as long as it can be used for cultivation of mushroom fungi in a container with a fungus bed. For example, a powdered material such as sawdust is used as a main material, and rice bran is used. It is obtained by mixing secondary ingredients containing a relatively large amount of nutrients, such as bran, and further adjusting the water content. Further, in the present invention, a nutrient corn germ meal (corn oil pomace) or other medium material together with sawdust, which is easily restored even when compressed at the time of inoculation hole formation, or expanded by heating during autoclave sterilization treatment Easy-to-use materials can be used. Alternatively, other media materials can be used instead of sawdust.

The container in which the culture medium for mushroom cultivation according to the present invention is made of a bottle or bag made of a heat-resistant synthetic resin, glass, ceramics or the like, and does not particularly specify the material or shape of the container. However, for containers such as bottles having a cylindrical opening, the culture medium expands by heating during sterilization in an autoclave and easily overflows from the container opening to the outside. Appropriate.

The material for forming the tool for forming an inoculation hole of the present invention may be prepared by heating at the time of sterilization in an autoclave (about 120 ° C. for 1 to 2 hours, or
It is a heat-resistant material that can withstand
Synthetic resins such as polypropylene, other heat-resistant synthetic resins (thermoplastic resins or thermosetting resins), glass, ceramics, and the like satisfy these heat-resistant conditions.

An embodiment of the present invention will be described below with reference to the drawings. As shown in FIGS. 1 and 2, the inoculation hole forming tool A ₁ of the first embodiment includes an inoculation hole 10 for a culture medium B from an opening 2 of a bottle-shaped polypropylene resin container 1 filled with a mushroom culture medium B. And a rod-shaped core 3 that can be inserted into
The rod-shaped core 3 made of polypropylene resin has a conical shape whose diameter becomes smaller as approaching the lower end, and a disc-shaped flange 4 is integrally formed with polypropylene resin on the upper part. . Further, a connecting shaft 5 is integrally formed so as to protrude from the center of the upper end surface of the flange 4 in the coaxial direction (upward) with the rod-shaped core 3, and the container opening 2 can be closed with the connecting shaft 5. A lid (cap) 6 made of a suitable polypropylene resin is detachably provided.

A pair of small plate-like projections 7 is provided on the upper peripheral edge of the connecting shaft 5 so as to be opposed to each other. A cylindrical portion 8 into which the connecting shaft 5 can be inserted is formed.

[0024] With this structure, when using inoculate hole tooling A ₁ is the connecting shaft 5 inserted into the plate-shaped small projection 7 with bayonet cutout 9 in alignment cylindrical portion 8 of the connecting shaft 5, These can be twisted and engaged with each other by a half turn to be appropriately integrated.

[0025] When using the seeded hole tooling A ₁ of the embodiment described above, the rod-shaped core 3 is inserted into the inoculation hole 10 from the opening 2 of the container 1 formed beforehand in the medium B, the surface of the medium B The flange 4 is pressed to cover the lid 6 (shown by a chain line in FIG. 2). Next, after sterilization in an autoclave, the medium B is cooled, and thereafter, the rod-shaped core 3 is withdrawn and inoculated with a seed fungus. In this way, the inoculation hole 10 is formed in the medium B to a required size, and the opening peripheral portion 11 of the inoculation hole 10 is formed in a planar shape.

In the second embodiment shown in FIG. 3, the connecting shaft 5 of the first embodiment (FIG. 1) is provided with a cylindrical connecting shaft 12 without providing the small plate-like projection 7. , Lid 13
A cylindrical portion 14 having a size capable of inserting the cylindrical connecting shaft 12 is formed on the rear surface of the cylindrical portion 14, and four wedge-shaped small projections 15 are formed on the inner peripheral edge of the cylindrical portion 14 at intervals and inserted. The connection shaft 12 can be frictionally engaged, and the other structure is exactly the same as that of the first embodiment.

In the inoculation hole forming tool A ₂ of the second embodiment, the lid 13, the connecting shaft 12 and the rod-shaped core 3 can be integrally connected only by inserting the connecting shaft 12 into the cylindrical portion 14. The rod-shaped core 3 can be removed from the lid 13 by the reverse operation, and the other usage can be performed in exactly the same manner as in the above-described first embodiment.

[0028]

【Example】

[Example 1] Rice bran and corn germ meal (corn oil pomace) as nutrients against cedar sawdust
Were mixed at 50% each to obtain a medium. As a control, a medium was also prepared in which rice bran was added to cedar sawdust as a nutrient.

The nutritional content of rice bran or a mixture of rice bran and corn germ meal with respect to cedar sawdust is set to be 90 g in dry weight per bottle, and the water content is 62 g.
% And mixed into a 850 ml polypropylene mushroom cultivation bottle, each of which was filled with 450 g of rice bran medium and 390 g of corn germ meal mixed medium per bottle.

Then, 16 cultivation bottles in which an inoculation hole was formed in the (cedar sawdust + rice bran) medium by the usual method were prepared, and the (cedar sawdust + rice bran + corn germ meal) mixed medium was filled into the cultivation bottles. Prepare 16 cultivation bottles with an inoculation hole by the usual method. After opening the inoculation hole, form the inoculation hole with a rod (without flange) of the same thickness as the rod-shaped core, and open the lid without removing the rod. 16 cultivation bottles were prepared, and 16 cultivation bottles were prepared with the rod-shaped core inserted in the mixed medium of (cedar sawdust + rice bran + corn germ meal) according to the procedure described in the above embodiment. did.

Next, the containers (cultivation bottles) containing the mushroom cultivation medium were sterilized in an autoclave at 121 ° C. for 60 minutes, allowed to cool to room temperature, and observed and evaluated as follows.

When the presence or absence of an inoculation hole was observed, the culture medium of a cultivation bottle using an inoculation hole forming tool in a mixed medium of (cedar sawdust + rice bran + corn germ meal) was able to confirm the inoculation hole, but the same (cedar sawdust + The inoculation hole in the cultivation bottle which did not use the inoculation hole forming tool in the rice bran + corn germ meal) medium was closed.

Further, the culture medium in the cultivation bottle in which the inoculation hole is formed with a rod (without a flange) having the same thickness as the rod-shaped core and the lid is covered without removing the rod, the surface of the culture medium (bacterial bed) around the inoculation hole rises. There was no room to inoculate the inoculum. Therefore, this medium could not be inoculated.

A medium capable of inoculating the inoculum obtained as described above was inoculated with a sawdust inoculant, Hirakutake bacterium (OH101, manufactured by Onuki Bacteria) in a clean bench. For inoculation, a part of the inoculum was dropped into the inoculation hole, the surface of the medium was covered with the inoculum, and each medium was inoculated with a similar amount of the inoculum.

In the inoculation hole forming tool of the present invention, a lid from which a rod-shaped core with a flange was removed was used.
Cultured in a culture room at 2 ° C and 60% humidity, apparently
The number of days required for mycelia to spread on the whole medium, the number of days required from harvesting the fruiting body after performing the fruiting body generation treatment, and the yield per bottle were measured and summarized in Table 1. Was.

[0036]

[Table 1]

As is clear from the results shown in Table 1, in a cultivation bottle using a mixed medium of (cedar sawdust + rice bran + corn germ meal) and having an inoculation hole formed in a usual manner, the culture was carried out.
No hyphae were found on day one.

On the other hand, in a cultivation bottle in which a rod-shaped core was inserted and covered with a lid according to the procedure described in the embodiment and autoclaved, and then inoculated with the inoculum, good results were obtained, which were not much different from the rice bran medium in the control plot. The number of days required for fruiting body development and the yield of fruiting bodies are not much different from those of the control group, and by using the tool for forming an inoculation hole of the mushroom cultivation medium of the present invention, a medium material that could not be used in normal fungal bed cultivation was used. It became clear that it could be used. In addition, it was also confirmed that the use of the flanged rod-shaped core can suppress the swelling of the bacterial bed surface after autoclave sterilization.

Example 2 90 g of bran was mixed with cedar sawdust in a dry weight per bottle, and further mixed with water so as to have a water content of 62%. Mushroom cultivation bottles were filled with a mixed medium in a quantity of 400 g per rice bran medium per bottle.

(Cedar sawdust + bran) Eight cultivation bottles having an inoculation hole formed in a medium by a usual method are prepared, and the inoculation hole forming tool of the above embodiment is used in a similar mixed medium. According to the procedure described, the rod-shaped core was inserted, and in this state, eight cultivation bottles capped were prepared. Next, 121 of these mushroom cultivation medium-containing containers (cultivation bottles) was added.
The mixture was sterilized in an autoclave at 60 ° C. for 60 minutes and allowed to cool to room temperature.

A medium capable of inoculating the inoculum obtained as described above was inoculated with a sawdust inoculant, Hirakutake bacterium (OH101, manufactured by Onuki Bacteria) in a clean bench. At the time of inoculation, a part of the inoculum was dropped into the inoculation hole, inoculated so that the surface of the medium was covered with the inoculum, and each medium was inoculated with the same amount of the inoculum.

In the inoculation hole forming tool of the present invention, a lid from which a rod-shaped core with a flange was removed was used.
C., cultivation in a culture chamber at 60% humidity, apparently the number of days required for mycelia to spread throughout the medium, and the number of days required from harvesting fruiting bodies after performing fruiting body development treatment, And the yield per bottle was measured and summarized in Table 2.

[0043]

[Table 2]

As is evident from the results in Table 2, in the cultivation bottle using the medium used for ordinary oyster mushroom cultivation (cedar sawdust + bran), the use of the inoculation hole forming tool of the present invention allows the spread of hyphae. Days required to spread (spread days) have been reduced. In addition, no difference was observed in the number of days from the generation treatment of the fruiting body to the harvest (the number of treatment treatment days) and the yield of the fruiting body. Therefore, by using the inoculation hole forming tool of the present invention, the number of days of infestation was reduced, and the number of days required for cultivation was correspondingly reduced.

[0045]

As described above, the tool for forming an inoculation hole for a mushroom cultivation medium according to the present invention is provided with a rod-shaped core for forming an inoculation hole which can be inserted into the inoculation hole of the mushroom cultivation medium. A flange is provided so that the periphery of the opening of the inoculation hole can be formed into a flat shape.The rod-shaped core is inserted into the inoculation hole of the culture medium, and autoclaving is performed as it is. There is an advantage that the shape of the hole can be maintained.

Further, the surface shape of the culture medium in the vicinity of the opening of the container is adjusted by press molding by the flange, so that there is also an advantage that a space for inoculating the inoculum can be ensured in the opening of the container.

[Brief description of the drawings]

FIG. 1 is an exploded perspective view of a first embodiment.

FIG. 2 is a cross-sectional view of a container containing a culture medium illustrating a use state.

FIG. 3 is an exploded perspective view of the second embodiment.

[Explanation of symbols]

A ₁ , A ₂ Inoculation hole forming tool B Medium 1 Container 2 Opening 3 Bar-shaped core 4 Flange 5, 12 Connection shaft 6, 13 Lid 7 Plate small projection 8, 14 Cylindrical part 9 Notch for insertion 10 Inoculation hole 11 Opening Peripheral edge 15 Small wedge-shaped protrusion

Claims (3)

[Claims] An inoculation hole for a mushroom cultivation medium, comprising: a rod-shaped core which can be inserted into an inoculation hole of a mushroom cultivation medium; and a flange for forming the periphery of the opening of the medium in a flat shape. Molding tools. 2. The mushroom cultivation medium inoculation hole forming tool according to claim 1, wherein one end of the rod-shaped core is provided with a lid capable of closing an opening of the container containing the mushroom cultivation medium. Inoculation hole forming tool for cultivation medium. 3. The tool for forming an inoculation hole of a culture medium for mushroom cultivation according to claim 2, wherein the lid is detachably provided to the rod-shaped core.