JPH1042864A - Bacterium for decomposing terephthalic acid and aerobic treatment using the same bacterium - Google Patents

Abstract

PROBLEM TO BE SOLVED: To treat a waste fluid, etc., of a polyester fiber fabric from a caustic reduction treatment with an alkali by only minimum dilution and cooling by aerobically treating a terephthalic acid-containing waste liquid with a specific microorganism belonging to the genus Pseudomonas. SOLUTION: This terephthalic acid decomposing bacterium belongs to the genus Pseudomonas and has terephthalic acid decomposing ability at >=40 deg.C, e.g. Pseudomonas sp. C4S strain (FERM P-15544). Terephthalic acid-containing waste fluid is aerobically treated with the bacterium. Terephthalic acid can rapidly be decomposed at a high temperature of 40-55 deg.C even if the waste fluid contains high-concentration terephthalic acid.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for treating a waste liquid containing terephthalic acid, particularly a waste liquid for processing a polyester fiber fabric by alkali reduction.

[0002]

2. Description of the Related Art Waste liquid discharged from processing and manufacturing processes of polyester fibers and polyester films contains a large amount of terephthalic acid. In particular, in the process of reducing the weight of polyester fibers with sodium hydroxide, high-temperature, high-concentration terephthalic acid-containing waste liquid is generated.

At present, waste liquids containing a large amount of terephthalic acid are generally treated by an activated sludge method. However, a high concentration of terephthalic acid tends to cause filamentous bulking, and it is necessary to dilute the waste liquid. Due to the fact that terephthalic acid is rather difficult to decompose, it is necessary to make the activated sludge treatment facility requiring a large treatment space wider, and it cannot be said that terephthalic acid is treated efficiently.

[0004] In recent years, the amount of waste liquid for alkali reduction processing has also increased due to the spread of new synthetic fibers. However, in a general activated sludge treatment facility having the above characteristics, the amount of wastewater to be treated is increased due to the problem of additional space. It is difficult to respond flexibly to the increase in volume. As a method for treating terephthalic acid-containing waste liquid, there are cases where terephthalic acid is separated by acid precipitation and the remaining waste liquid is treated by the activated sludge method, etc. It cannot be said that it is superior.

Recently, an ozone treatment (Japanese Patent Laid-Open No. 6-26979)
7) and wet oxidation treatment (Japanese Patent Application Laid-Open No. 7-204666) are disclosed. However, the former requires the use of ozone, and the latter requires the use of a catalyst and treatment under high-temperature and high-pressure conditions. Therefore, it is questionable whether this method can be an excellent method in terms of cost and ease of management.

On the other hand, as for microorganisms that degrade terephthalic acid, microorganisms belonging to the genus Nocardia have an initial concentration of 0.
Report that 25% of terephthalic acid was decomposed to 0.09% in 24 hours (FEMS, Microbiology.
Letters, Vol. 5, pp. 245-251, 1979), a report (FEM) that a microorganism belonging to the genus Bacillus degraded terephthalic acid at a concentration of 0.2% in 24 hours.
S. Microbiology Letters, Vol. 30, 217
~ 220 pages, 1985).
Particularly in the genus Pseudomonas, microorganisms that degrade terephthalic acid are known in Pseudomonas alkaline genes and the like (Proc. Indian Acad. Sci., 93
Vol. 1155-1158, 1984).
There is no report that terephthalic acid was aerobically treated with microorganisms at a temperature of at least 50 ° C, especially at least 50 ° C.

[0007]

SUMMARY OF THE INVENTION An object of the present invention is to increase the efficiency of the treatment of terephthalic acid-containing waste liquid. In particular, by developing a technology that minimizes the dilution and cooling of alkali-weight reduction processing wastewater of polyester fiber fabrics containing terephthalic acid at high concentration and high temperature, and develops a technology that can treat the wastewater under high load, Is to speed up, save space, and reduce costs.

[0008]

SUMMARY OF THE INVENTION The gist of the present invention which has solved the above-mentioned problems is basically "a terephthalic acid-decomposing bacterium belonging to the genus Pseudomonas and capable of decomposing terephthalic acid at a temperature of 40 ° C. or more". It is in.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The basis of the present invention is to utilize a terephthalic acid-decomposing microorganism which has a high terephthalic acid decomposition rate and can grow even at a temperature of 40 ° C. or higher. Preferably, 40
To 70 ° C, more preferably 45 to 60 ° C. 40 ℃
This is because, in the following, the decomposition rate decreases and the growth of other germs becomes remarkable. The microorganism of the present invention is at 40 ° C.
It is an essential condition that terephthalic acid can be decomposed at the above temperature, and terephthalic acid can be decomposed at a temperature of 40 ° C. or lower. The term "terephthalic acid decomposing ability" means, for example, the ability to decompose 0.5% of terephthalic acid within 50 days or more within 2 days and 95% or more within 5 days.

Although not particularly limited, such terephthalic acid-decomposing bacteria belong to the genus Pseudomonas,
Pseudomonas sp., A microorganism capable of decomposing terephthalic acid
Terephthalic acid-decomposing bacteria, which is a C4S strain (Biotechnological Laboratory No. 15544), is preferred. Hereinafter, the present invention will be described using this microorganism as an example.

The microbiological properties of the microorganisms obtained above are shown below.

(A) Morphology (juice agar medium, cultured at 50 ° C.) (1) Cell shape and size: Bacillus, 0.5-0.7 × 0.9-1.2 μm (2) Motility (flagellate): Yes (one (3) Spores: no (4) Gram stainability:-(5) Acid resistance:-(b) Growth state (1) Gravy agar plate culture: Bright nautical color. (In a medium using terephthalic acid as a carbon source, the color tone approaches white.) Circular, whole edge, convex circle or central convex shape. About 2 mm in diameter in 3 days.

(2) Gravy liquid culture: Medium turbidity.

(3) Broth gelatin puncture culture: No liquefaction.

(4) Milk: does not coagulate.

(C) Physiological properties (1) Reduction of nitrate:-/ + (2) Denitrification reaction:-(3) MR test:-(4) VP test:-(5) Formation of indole:-( 6) Production of hydrogen sulfide:-(7) Hydrolysis of starch:-(8) Use of citric acid Medium of Koser: + Medium of Christensen: + (9) Use of inorganic nitrogen source Ammonium salt: + Nitrate:-( 10) Pigment production:-(11) Urease:-(12) Oxidase: + (13) Catalase: + (14) DNase production:-(15) Growth range Temperature: Upper limit 57 ° C pH: 6.0 to 10.0 ( 16) Attitude to oxygen: aerobic (17) OF test:-(18) Acid and gas generation from sugar: D-glucose, L-arabinose, D-xylose, D-mannose, D-fructose, D -Galactose, maltose, sucrose, lactose, trehalose, D-sorbitol , D- mannitol, acid from Izure inositol, of glycerin, do not produce gas.

(19) Utilization (+): phthalic acid, isophthalic acid, terephthalic acid, succinic acid, gentisic acid (-): ethylene glycol, n-propanol, ethanol, ethanolamine, betaine, lactic acid, benzoic acid, p-Methylbenzoic acid, p-methoxybenzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, vanillic acid, phenol Results are in Bargeys-Manual-of-Systematic-Bacteriology vol.1 (1984) As a result of the comparison, the C4S strain is a gram-negative aerobic bacterium, which is catalase and oxidase-positive, and has polar flagella. Is close to Pseudomonas pseudo-alka-genes There was found. Therefore, as a result of comparison with Pseudomonas alkaline genes IFO 14159 and Pseudomonas pseudo alkaline genes IFO 14167, the C4S strain can grow at a temperature of 55 ° C., can utilize terephthalic acid, does not use lactic acid, etc. The C4S strain was considered to be a new species of Pseudomonas because it was different from both strains. Therefore, it was identified as Pseudomonas sp. (Biotechnological Laboratory No. 15544).

The mode of the waste liquid treatment is not particularly limited, and Pseudomonas sp. C4S may be used for the terephthalic acid-containing waste liquid alone, or may be mixed with another waste liquid if necessary. good. From the waste liquid, the one containing a large amount of terephthalic acid was recovered, and Pseudomonas sp. C4S
After completely decomposing the terephthalic acid component or decomposing most of the terephthalic acid component, it may be subjected to other general microorganism treatment. Pseudomonas sp. C4S can be used in suspension or
It may be used by immobilizing it on a comprehensive immobilizing carrier, activated carbon or the like. The processing temperature is preferably 30 ° C to 55 ° C, particularly preferably 40 ° C to 50 ° C.

The waste liquid to be treated in the present invention contains terephthalic acid, and is typically a terephthalic acid production step and a waste liquid for alkali reduction of polyester fibers. The waste liquid contains ethylene glycol, sodium hydroxide, other inorganic substances and organic substances other than terephthalic acid, but these do not hinder the decomposition treatment ability of the present invention. If necessary, aqueous ammonia, ammonium sulfate, ammonium salt, ammonium phosphate, sodium nitrate, ammonium nitrate, potassium nitrate, meat extract, yeast extract, compounds selected from organic and inorganic nitrogen sources such as peptone, further magnesium salt, potassium salt, A compound selected from phosphoric acid (salt), iron salt, other metal salt, and the like is added.

The waste liquid treated in the present invention preferably contains terephthalic acid in an amount of 0.5% or more, more preferably 0.6% or more, and further preferably 0.8% or more.
That is all. The upper limit is not particularly limited, but is preferably 4.0% or less, more preferably 3.7% or less.

The ratio of terephthalic acid to nutrients or carbon sources in terms of BOD is not particularly limited, but is preferably 21% or more, more preferably 63% or more, and further preferably 70%. Above, particularly preferably at least 74%. Further, ethylene glycol may be contained as another carbon source in the waste liquid to be treated.

[0022]

【Example】

[Example 1] Terephthalic acid 0.2%, monopotassium phosphate 0.15
%, Magnesium sulfate heptahydrate 0.02%, ferrous sulfate heptahydrate 0.002%, bactotripton (Difco) 0.2%, yeast extract (Difco) 0.2%, pH 6.5 liquid medium, agar of the same composition C4S strain growing on the culture medium
No. 5544) was inoculated with a platinum loop, and 30 ° C., 45 ° C., 50
C., the terephthalic acid in the medium was increased to 45% or more, 95% or more,
% Or more.

Example 2 0.6% of terephthalic acid, 0.15% of monopotassium phosphate, 0.02% of magnesium sulfate heptahydrate, 0.002% of ferrous sulfate heptahydrate, bactotripton (Difco)
A platinum loop of C4S strain (Biotech No. 15544) grown on an agar medium of the same composition was inoculated into a liquid medium of 0.2%, yeast extract (Difco) 0.2%, pH 6.5 and inoculated at 50 ° C. After shaking, the culture was cultured for 30 hours, 50 hours, 60 hours.
The terephthalic acid in the medium is more than 50%,
It was decomposed by 0% or more and 97% or more.

EXAMPLE 3 0.6% terephthalic acid, 0.20% monopotassium phosphate, 0.02% magnesium sulfate heptahydrate, 0.002% ferrous sulfate heptahydrate, 0.5% ammonium sulfate, 0.02% yeast extract (Difco) %, EDTA 2μM, pH 6.5, C4S subcultured at 45 ° C using the same medium
2% vol / vol of the strain (Biotech No. 15544)
, And cultured with shaking at 45 ° C. After 24 hours and 42 hours of culture, terephthalic acid was 43% each.
%, 88%.

[0025]

According to the present invention, a terephthalic acid-decomposing bacterium which belongs to the genus Pseudomonas and has a terephthalic acid-decomposing ability at a temperature of 40 ° C. or more, in particular, a terephthalic acid-containing waste liquid can be directly subjected to medium-high temperature conditions by using Pseudomonas sp. Aerobic treatment has become possible, and the efficiency of treating waste liquid containing terephthalic acid has become possible. In particular, the present microorganism works effectively when high-concentration, high-temperature terephthalic acid-containing waste liquid is generated, for example, in an alkali weight reduction treatment of a polyester fiber fabric. For example, a compact tank for aerobically cultivating Pseudomonas sp. C4S is provided to treat high-concentration effluents and reduce the load significantly. By biological treatment, the wastewater treatment capacity of the entire facility can be greatly increased by low-cost and space-saving expansion. When a new waste liquid treatment facility is newly established, it is possible to significantly reduce the size of the treatment facility as a whole. Since Pseudomonas sp. C4S can grow even at a high temperature, it is possible to maintain the temperature at 40 ° C. to 55 ° C., more preferably 45 ° C. to 50 ° C., when treating high concentration waste liquid. It is possible to save or eliminate the cooling cost for the heat of the waste liquid and the heat generated during the aerobic treatment. In addition, since autologous digestion of microorganisms is promoted as compared with around 30 ° C., excess sludge can be reduced and the amount of added nutrients can be saved. Even when terephthalic acid-containing wastewater, particularly high-concentration wastewater, is discharged into sewers, similar effects can be expected by using Pseudomonas sp. C4S for the purpose of load reduction treatment and preliminary treatment. By introducing Pseudomonas sp. C4S alone or in the form of entrapping immobilization carriers into general biological treatment tanks such as activated sludge tanks, the decomposition and treatment of terephthalic acid, which is slightly hard to decompose, is promoted. It became possible. In particular, it has become possible to prevent processing defects at high water temperatures.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical display location C12R 1:38)

Claims (4)

[Claims] 1. A terephthalic acid-decomposing bacterium belonging to the genus Pseudomonas and capable of decomposing terephthalic acid at a temperature of 40 ° C. or higher. 2. A terephthalic acid-degrading bacterium belonging to the genus Pseudomonas and having the ability to degrade terephthalic acid, which is a strain Pseudomonas sp. C4S (Biotechnical Research No. 15544). 3. A method for aerobically treating terephthalic acid-containing waste liquid, comprising using the microorganism according to claim 1. 4. The aerobic treatment method for terephthalic acid-containing waste liquid according to claim 2, wherein the treatment temperature is 40 to 55 ° C.