JP4058771B2 - Treatment of terephthalic acid-containing waste liquid - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for treating a terephthalic acid-containing waste liquid, in particular, an alkali weight reduction processing waste liquid for polyester fiber fabrics.
[0002]
[Prior art]
Waste liquid discharged from processing and manufacturing processes of polyester fibers and polyester films contains a large amount of terephthalic acid. In particular, in a weight reduction processing step of polyester fiber with sodium hydroxide, a high-temperature, high-concentration terephthalic acid-containing waste liquid is generated.
[0003]
Currently, waste liquids that contain a large amount of terephthalic acid are generally treated by the activated sludge process. However, terephthalic acid tends to cause filamentous bulking at a high concentration, and it is necessary to perform a dilution operation of the waste liquid. For the reason that it is somewhat difficult to decompose, it is necessary to make the activated sludge treatment facility that requires a wide treatment space wider, and it cannot be said that it is treated efficiently.
[0004]
In addition, due to the spread of new synthetic fibers, the amount of waste liquid from alkaline weight reduction processing has increased in recent years. However, in general activated sludge treatment facilities having the above characteristics, the amount of treatment increased due to the problem of additional space. It is difficult to respond flexibly to the situation. As a treatment method for terephthalic acid-containing waste liquid, there are cases in which terephthalic acid is separated by acid precipitation, and the remaining waste liquid is treated by the activated sludge method. It cannot be said that it is superior.
[0005]
Recently, ozone treatment (JP-A-6-2699797) and wet oxidation treatment (JP-A-7-204666) have been disclosed, but the former requires the use of ozone, the latter requires the use of a catalyst, and treatment under high-temperature and high-pressure conditions. Therefore, it is doubtful whether it can be an excellent method in terms of cost and ease of management as compared with the activated sludge method.
[0006]
On the other hand, with regard to microorganisms that degrade terephthalic acid, a report that microorganisms belonging to the genus Nocardia decomposed terephthalic acid with an initial concentration of 0.25% to 0.09% in 24 hours (FEMS Microbiology Letters, 5, 245-251, 1979), a report with a description that microorganisms belonging to the genus Bacillus decomposed terephthalic acid at a concentration of 0.2% in 24 hours (FEMS Microbiology Letters, 30, 217-220 pages (1985) .
Also in shea Yudomonasu genus, such as in Pseudomonas alcaligenes, microorganisms degrade terephthalic acid is known (Proc. Indian Acad. Sci. , 93 , pp. 1155-1158, 1984), but the treatment temperature Is 30 ° C. Treatment temperature in these prior art is at most 41 ° C., 45 ° C. or higher, particularly Oite to high temperatures, such as on 50 ° C. or more, using a microorganism, reports that were treated terephthalic acid aerobically is Absent.
[0007]
[Problems to be solved by the invention]
An object of the present invention is to increase the efficiency of treatment of terephthalic acid-containing waste liquid. In particular, we developed a technology that can treat the waste liquid from alkaline fiber weight reduction processing of polyester fiber fabrics containing high-concentration and high-temperature terephthalic acid, with minimal dilution and cooling. Speed, space saving, and cost reduction.
[0008]
[Means for Solving the Problems]
This onset Akira which solves such problems, using the "Pseudomonas of terephthalic acid decomposition ability belonging to the city Yudomonasu the genus sp. C4S strain (Life Engineering Research microorganisms nearest No. 15544), terephthalic acid containing at a temperature of 45~57 ℃ It is in "Aerobic treatment of waste liquid" .
[0009]
DETAILED DESCRIPTION OF THE INVENTION
The basis of the present invention is that a terephthalic acid-decomposing microorganism that has a high terephthalic acid decomposition rate and can grow at a temperature of 45 to 57 ° C. is used. Preferably, it is 45-55 degreeC . This is because if the temperature is lower than 45 ° C. , the degradation rate decreases or the growth of other miscellaneous bacteria becomes significant. Note that the microorganism of the present invention is required to have a terephthalic acid decomposing ability at a temperature of 45 ° C. or higher, and the terephthalic acid decomposing ability at a temperature of less than 45 ° C. is not limited. The terephthalic acid decomposing ability means, for example, that 0.5% terephthalic acid has an ability to decompose 50% or more within 2 days and 95% or more within 5 days.
[0010]
The terephthalic acid-degrading bacteria that written, belong to the genus Pseudomonas, Pseudomonas microorganisms of terephthalic acid decomposition ability sp. C4S stock using a terephthalic acid-degrading bacteria is (life Engineering Institute microorganisms nearest No. 15544). Hereinafter, the present invention using this microorganism will be described.
[0011]
The following are the mycological properties of the obtained microorganisms.
[0012]
(A) Morphology (meat broth agar, cultured at 50 ° C)
(1) Shape and size of cells: Neisseria gonorrhoeae, 0.5-0.7 × 0.9-1.2μm
(2) Motility (flagella): Existence (single polar flagellum)
(3) Spore: None (4) Gram staining: −
(5) Anti-acid: −
(B) Growth state (1) Meat broth agar plate culture: Bright noodle color. (In a medium using terephthalic acid as a carbon source, the color tone approaches white.) Circular, full edge, convex circle, or central convex shape. About 2 mm in diameter in 3 days.
[0013]
(2) Meat broth liquid culture: Medium turbidity.
[0014]
(3) Gravy gelatin puncture culture: Does not liquefy.
[0015]
(4) Milk: Does not coagulate.
[0016]
(C) Physiological properties (1) Reduction of nitrate:-/ +
(2) Denitrification reaction: −
(3) MR test: −
(4) VP test: −
(5) Production of indole: −
(6) Production of hydrogen sulfide: −
(7) Starch hydrolysis: −
(8) Use of citric acid
Koser's medium: +
Christensen's medium: +
(9) Use of inorganic nitrogen source Ammonium salt: +
Nitrate: −
(10) Dye production: −
(11) Urease: −
(12) Oxidase: +
(13) Catalase: +
(14) DN ace production: −
(15) Growth temperature range: Upper limit 57 ° C
pH: 6.0 to 10.0
(16) Attitude toward oxygen: Aerobic (17) OF test: −
(18) Generation of acid and gas from sugar:
Acids from any of D-glucose, L-arabinose, D-xylose, D-mannose, D-fructose, D-galactose, maltose, sucrose, lactose, trehalose, D-sorbitol, D-mannitol, inosit, glycerin Does not produce gas.
[0017]
(19) Associability (+):
Phthalic acid, isophthalic acid, terephthalic acid, succinic acid, gentisic acid (-):
Ethylene glycol, n-propanol, ethanol, ethanolamine, betaine, lactic acid, benzoic acid, p-methylbenzoic acid, p-methoxybenzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, vanillic acid, phenol results C4S was a Gram-negative aerobic bacilli and positive for catalase and oxidase and polar flagellum, as a result of comparison with Barjays-Manual-of-Systematic-Bacteriology vol.1 (1984). Since no fluorescent dye was produced and glucose was not used, it was found that Pseudomonas alkali genes and Pseudomonas pseudoalkali genes are closely related. Therefore, as a result of comparison with Pseudomonas Alkaline Genes IFO 14159 and Pseudomonas Pseudo Alkaline Genes IFO 14167, the C4S strain can be grown at a temperature of 55 ° C, can utilize terephthalic acid, does not use lactic acid, etc. Because it was different from both strains, the C4S strain was considered to be a new species of Pseudomonas. Therefore, it was identified as Pseudomonas sp. (Biotechnological Microorganism No. 15544).
[0018]
The manner of waste liquid treatment is not particularly limited, and Pseudomonas sp. C4S may be used for the terephthalic acid-containing waste liquid alone, or other waste liquids may be mixed and treated as necessary. Of the waste liquid, one containing a large amount of terephthalic acid may be recovered, and the terephthalic acid component may be completely decomposed or most decomposed using Pseudomonas sp. C4S, and then subjected to other general microbial treatments. . Pseudomonas sp. C4S may be used in a suspended state, or may be used by being fixed to a entrapping immobilization carrier, activated carbon or the like. The treatment temperature is 45 ° C to 57 ° C, and 45 ° C to 55 ° C is particularly preferable.
[0019]
The waste liquid to be treated in the present invention contains terephthalic acid, and is typically a terephthalic acid production process, an alkali weight reduction processing waste liquid for polyester fibers. The waste liquid contains ethylene glycol, sodium hydroxide, other inorganic substances, and organic substances in addition to terephthalic acid, but these do not interfere with the decomposition treatment ability of the present invention. If necessary, compounds selected from organic and inorganic nitrogen sources such as aqueous ammonia, ammonium sulfate, ammonium chloride, ammonium phosphate, sodium nitrate, ammonium nitrate, potassium nitrate, meat extract, yeast extract, peptone, magnesium salt, potassium salt, A compound selected from phosphoric acid (salt), iron salt, other metal salts, and the like is added.
[0020]
The waste liquid to be treated in the present invention preferably contains 0.5% or more of terephthalic acid, more preferably 0.6% or more, and further preferably 0.8% or more. Moreover, although it does not specifically limit regarding an upper limit, Preferably it is 4.0% or less, More preferably, it is 3.7% or less.
[0021]
Further, the proportion of terephthalic acid in the nutrient source or carbon source in terms of BOD is not particularly limited, but is preferably 21% or more, more preferably 63% or more, still more preferably 70% or more, particularly Preferably it is 74% or more. Moreover, ethylene glycol may be contained as another carbon source in the waste liquid to be treated.
[0022]
【Example】
[Example 1]
Terephthalic acid 0.2%, monopotassium phosphate 0.15%, magnesium sulfate heptahydrate 0.02%, ferrous sulfate heptahydrate 0.002%, bactotryptone (Difco) 0.2%, yeast extract (Difco) 0.2%, pH A liquid medium of 6.5 was inoculated with a platinum loop of C4S strain (Life Technologies Laboratories No. 15544) growing on an agar medium of the same composition, and cultured with shaking at 30 ° C, 45 ° C and 50 ° C. In 30 hours of cultivation, terephthalic acid in the medium was decomposed by 45% or more, 95% or more, and 95% or more, respectively.
[0023]
[Example 2]
0.6% terephthalic acid, 0.15% monopotassium phosphate, 0.02% magnesium sulfate heptahydrate, 0.002% ferrous sulfate heptahydrate, 0.2% bactotryptone (Difco), 0.2% yeast extract (Difco), pH A platinum medium was inoculated into 6.5 liquid medium with a C4S strain (Life Engineering Laboratories No. 15544) growing on an agar medium of the same composition and cultured at 50 ° C. with shaking. In 60 hours, terephthalic acid in the medium was decomposed by 50% or more, 80% or more, and 97% or more, respectively.
[0024]
[Example 3]
Of terephthalic acid 0.6%, monopotassium phosphate 0.20%, magnesium sulfate heptahydrate 0.02%, ferrous sulfate heptahydrate 0.002%, ammonium sulfate 0.5%, yeast extract (Difco) 0.02%, EDTA 2μM, pH 6.5 A C4S strain (Life Technologies Laboratories No. 15544) subcultured at 45 ° C was inoculated in a liquid medium at a rate of 2% vol / vol and cultured at 45 ° C with shaking. In 24 hours and 42 hours of culture, terephthalic acid was degraded by 43% and 88%, respectively.
[0025]
【The invention's effect】
Belong to the genus Pseudomonas, the terephthalic acid-containing waste liquid by using the Oh Cie Yudomonasu sp. Terephthalic acid degrading bacteria is C4S of terephthalic acid decomposition ability, at medium high temperature (45-57 ° C.) Article matter under direct good It has become possible to treat the waste liquid efficiently, making it possible to improve the efficiency of the treatment of waste liquid containing terephthalic acid. In particular, alkali reduction processing of a polyester fiber fabric, a high concentration, acts effectively in cases with generation of high-temperature terephthalic acid-containing waste liquid. For example, after installing a compact tank in which Pseudomonas sp. C4S is aerobically cultured, treating high-concentration waste liquid, and reducing the load significantly, the treatment liquid and the remaining waste liquid are mixed with a general method such as the activated sludge method. Biological treatment has made it possible to significantly increase the waste liquid treatment capacity of the facility as a whole at low cost and space saving. When a new waste liquid treatment facility is newly established, the treatment facility as a whole can be greatly downsized.
Pseudomonas sp. C4S can grow even at medium and high temperatures, so when processing high concentration waste liquor, it is possible to maintain the temperature at 45 ° C to 57 ° C, more preferably 45 ° C to 50 ° C. It is possible to save or eliminate the cooling cost for the heat of the waste liquid and the heat generated during the aerobic treatment. In addition, since the self-digestion of microorganisms is promoted as compared to around 30 ° C., it is possible to reduce excess sludge and save the amount of nutrient salts added. The same effect can be expected if Pseudomonas sp. C4S is used for load-reducing treatment or preliminary treatment, even when terephthalic acid-containing wastewater, particularly high-concentration wastewater, is discharged into sewers. Introducing Pseudomonas sp. C4S into general biological treatment tanks such as activated sludge tanks alone or in the form of a entrapped immobilization carrier, etc., promotes the decomposition and treatment of terephthalic acid, which is slightly difficult to decompose. It became possible. In particular, it has become possible to prevent treatment failures at high water temperatures.

Claims (1)

Pseudomonas is a terephthalic acid degrading bacteria belonging to the genus Pseudomonas sp. C4S using strain (Life Engineering Institute microorganism preferred No. 15544), comprises treating aerobically terephthalic acid-containing waste liquid at a temperature of 45-57 ° C. A method for treating waste liquid containing terephthalic acid.