JPH10179731A - Blood treating device and blood treatment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To make it possible to rapidly treat blood by a simple operation and to effectively function an anticoagulant without excess or shortage. SOLUTION: This blood treating device 20 has a blood treating means 1 comprising a vessel 6 which stores the treated blood, a cell removing filter 4 which selectively removes, for example, white cells, a first tube 3 which introduces the blood to the cell removing filter 4, a puncture needle which is mounted at the front end of the first tube 3, a second tube 5 which introduces the blood past the cell removing filter 4 to the vessel 6, a third tube 8 which is joined to a branching part 13 disposed in mid-way of the first tube 3 and supplies the anticoagulant and an anticoagulant vessel 7 which is connected to the end of the third tube 8. Further, the device has a flow rate detecting means 30 which detects the blood flow rate in the first tube 3 and a control means which controls the supply rate of the anticoagulant by opening and closing a chuck 23 at prescribed timing according to the detected blood flow rate.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a blood processing apparatus and a blood processing method for performing a blood processing for separating a specific blood component from blood.

[0002]

2. Description of the Related Art There is known a blood processing system that uses a leukocyte removal filter capable of selectively removing leukocytes, separates and removes leukocytes from blood, and collects the blood from which leukocytes have been removed in a bag (Japanese Patent Publication (Kokai) No. Hei 9-163). 6-59305, Tokuhei 6
-59304 etc.).

In this system, a first bag for storing collected blood and a second bag for storing blood subjected to leukocyte removal processing (hereinafter simply referred to as “processing” or “blood processing”).
Bag and tube connecting both bags (blood flow path)
And a blood processing device comprising a leukocyte removal filter provided in the middle of the tube.

[0004] In this case, an appropriate amount of anticoagulant liquid corresponding to the amount of blood to be collected is previously put in the first bag together with the blood to be collected, and the mixture passes through a leukocyte removal filter in a mixed state. This is because, when the blood passes through the leukocyte removal filter, if the anticoagulant liquid is not mixed into the blood, a thrombus due to the coagulation of the blood occurs, and the pores of the filter medium are clogged.

However, such a conventional system has various disadvantages as follows.

[0006] Since blood to be passed through the leukocyte removal filter must be previously mixed with an anticoagulant in order to prevent coagulation of the blood, blood collected from a donor (donor) is immediately used as it is. That is, it cannot be processed without temporarily storing it in a bag or the like. Therefore, the operation of storing the collected blood in the first bag and the operation of discharging the blood from the first bag and processing the blood must be performed separately, which complicates the operation.

[0007] The time (leaving time) from the blood collection to the leukocyte removal treatment becomes longer, and as this time becomes longer, the red blood cell recovery rate tends to decrease.

[0008] The presence of the first bag increases the size of the whole blood processing device. For this reason, it is disadvantageous in handling, packaging, transportation, and the like at the time of blood collection and the like, and when using a vacuum blood collection device or an oscillating blood collection device, it may be difficult to store the blood processing tool in an existing device.

In addition, it is conceivable to inject and mix an anticoagulant into a collected blood at a preset speed, while treating the mixture. However, since the blood collection speed is not constant, the mixing ratio between the blood and the anticoagulant may be reduced. Variations may occur, and the antithrombotic properties of the leukocyte removal filter may not be sufficiently exhibited.

[0010]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a blood processing method and a blood processing method capable of rapidly processing blood with a simple operation and enabling an anticoagulant to function effectively without excess or deficiency. An object of the present invention is to provide a processing device.

[0011]

This and other objects are achieved by the present invention which is defined below as (1) to (12).

(1) A container for storing the processed blood, a cell removal filter for selectively removing predetermined cells in the blood, and a first filter for introducing blood into the cell removal filter.
A flow path, a second flow path for introducing the blood that has passed through the cell removal filter into the container, a third flow path communicating with the first flow path, and supplying an anticoagulant solution, Flow rate detection means for detecting the flow rate of blood flowing through the first flow path or the second flow path; and control for controlling the supply amount of the anticoagulant liquid according to the flow rate detected by the flow rate detection means And a blood treatment apparatus.

(2) The blood processing apparatus according to the above (1), further comprising an anticoagulant container that communicates with the upstream side of the third flow path and stores the anticoagulant liquid.

(3) The control means includes a flow path opening and closing mechanism for opening and closing the third flow path, and a control unit for controlling the operation of the flow path opening and closing mechanism. 2)
A blood processing apparatus according to claim 1.

(4) The blood treatment according to (1) or (2), wherein the control means includes a pump for feeding the anticoagulant solution, and a control unit for controlling the discharge amount of the pump. apparatus.

(5) The blood processing apparatus according to (4), wherein the pump is a roller pump.

(6) The blood processing apparatus according to any one of (1) to (5), wherein the cell removal filter is a leukocyte removal filter capable of filtering at least leukocytes.

(7) The leukocyte removal filter is 40
(6) The blood processing apparatus according to the above (6), which has a performance such that when 0 ml of blood is processed, the number of white blood cells in the processed blood is less than 1 × 10 ⁸ .

(8) A blood processing method in which blood is passed through a cell removal filter to remove specific cells in the blood, and the blood from which the cells have been removed is collected in a container. A blood processing method comprising: detecting a flow rate of blood to be processed; and supplying an anticoagulant solution in an amount corresponding to the flow rate to an upstream side of the cell removal filter.

(9) A container for storing the processed blood, a cell removing filter for selectively removing predetermined cells in the blood, and a first filter for introducing blood into the cell removing filter.
A second flow path for introducing blood that has passed through the cell removal filter into the container, and a third flow path that communicates with the first flow path and supplies an anticoagulant solution. After the blood introduced from the first flow path is passed through the cell removal filter to selectively remove predetermined cells in the blood, the blood from which the cells have been removed is used. When performing the blood treatment to collect in the container through the second flow path, the flow rate of blood flowing through the first flow path or the second flow path is detected, and the amount of blood corresponding to the flow rate is detected. A blood processing method, wherein an anticoagulant solution is supplied through the third flow path and mixed with blood before being processed by the cell removal filter.

(10) The blood processing method according to the above (9), wherein the blood processing device has an anticoagulant container that communicates with the upstream side of the third flow path and stores the anticoagulant liquid.

(11) The blood processing method according to the above (10), further comprising a step of aseptically connecting the anticoagulant container to the third flow path as a pre-process.

(12) The blood processing method according to any one of (9) to (11), further comprising a step of aseptically connecting the container to the second flow path as a pre-process or a post-process.

[0024]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the blood processing method and the blood processing apparatus of the present invention will be described below with reference to the accompanying drawings.

FIGS. 1 to 12 are schematic views each showing an example of the configuration of the blood processing device 1 used in the present invention. Hereinafter, the configuration of the blood processing device 1 will be described based on these drawings.

The blood processing apparatus 1 shown in FIG. 1 has a container 6 for storing processed blood, a cell removing filter 4 for selectively removing predetermined cells in the blood, and introducing blood into the cell removing filter 4. First tube 3 forming a first flow path
, A puncture needle (blood collection needle) 2 attached to the tip of the first tube 3, and blood passing through the cell removal filter 4 in a container 6.
A second tube 5 constituting a second flow path to be introduced into the
A third tube 8 which communicates with the first flow path and forms a third flow path for supplying an anticoagulant solution, and an anticoagulant container 7 connected to an end of the third tube 8 It is configured.

The container 6 is located on the downstream side of the cell removing filter 4, and is constituted by a bag (blood bag) formed by fusing a soft sheet material made of, for example, polyvinyl chloride and forming it into a bag shape. The capacity of the container 6 is, for example, about 200 to 500 ml.

Similarly, the anticoagulant container 7 is also formed of a bag (anticoagulant bag) formed by fusing a soft sheet material to form a bag.

In the anticoagulant container 7, for example, ACD
Liquid, CPD liquid, CPDA-1 liquid, and anticoagulant liquid such as sodium heparin liquid.

The anticoagulant container 7 is, for example, 30 to 10
It has a relatively small volume of about 0 ml. In this embodiment,
The amount of the anticoagulant solution in the anticoagulant container 7 is set to an amount corresponding to one blood processing (blood collection) or a slightly larger amount.

The first tube 3, the second tube 5, and the third tube 8 are each made of a soft resin such as polyvinyl chloride, and have flexibility.

The third tube 8 is connected to the middle or the end of the first tube 3 via a branch portion 13 composed of a branch connector having a T-shape, a Y-shape, a T-shape or the like.

Although not shown, a breakable communication member (for example, click tip manufactured by Terumo Corporation) may be provided in the middle or at the end of the third tube 8, for example, to break and open and close the flow path. And the closed third flow path can be opened as needed (the same applies to the following examples). Further, such a break communication member can be installed also in the first tube 3 and the second tube 5. Further, a normal clamp (clamping member) may be used instead of these break communication members.

[0034] The cell removing filter 4 is used to filter a specific one of blood.
It is capable of selectively filtering and removing seeds or two or more kinds of cells. In this embodiment, a leukocyte removal filter for removing leukocytes or leukocytes and platelets is used. The configuration of the leukocyte removal filter will be described later in detail.

In the blood treatment device 1 shown in FIG. 2, the third tube 8 is not integrated from the beginning, and the anticoagulant container 7 is in a separated state. A third tube 8a on the anticoagulant container 7 side and a third tube 8b on the connection side with the first tube 3;
The aseptic connection device is used aseptically connected at a connection portion C1 shown in the figure. Other configurations are the same as those of the blood processing device 1 shown in FIG.

The aseptic connection device and the method of connecting tubes using the device are described, for example, in JP-B-61-30582, JP-B-3-11234, and JP-A-4-3087.
No. 31, JP-A-6-26877 and the like can be applied.

In the blood treatment device 1 having such a structure, the anticoagulant container 7 and other parts can be sterilized in a separate step in the manufacturing process, so that the cost can be reduced. High usefulness. Further, when packaging, transporting, storing, etc., the anticoagulant container 7 and the other relatively lightweight parts can be handled separately, which is convenient.

In the blood treatment device 1 shown in FIG. 3, the capacity of the anticoagulant container 7 is relatively large, and stores an anticoagulant solution in an amount corresponding to a plurality of blood treatments. Other configurations are the same as those of the blood processing device 1 shown in FIG.

In this blood processing device 1, the third tube 8
, The anticoagulant container 7 can be used for blood processing a plurality of times. In addition, since the anticoagulant container 7 is larger and heavier, the advantages in the above-described manufacturing, packaging, transportation, and storage by changing tubes are greater.

In the blood processing device 1 shown in FIG. 4, after the collection of the processed blood in the container 6 is completed, the tubes are aseptically connected at the connecting portion C2 shown in the figure using the aseptic connecting device. Thus, a sealed container 6 containing the processed blood is obtained, and the second tube 5 is connected to a new container 6. Other configurations are the same as those of the blood processing device 1 shown in FIG.

In the blood processing device 1, when the container 6 is in a predetermined state,
For example, since the blood can be separated after the end of the blood treatment, the advantages of the above-described manufacturing, packaging, transportation, and storage are greater than those of the configuration in FIG.

The blood processing device 1 shown in FIG. 5 has a container 6 connected to a container 6 via a tube 10 for storing blood components. Other configurations are the same as those of the blood processing device 1 shown in FIG.

The container 9 is a bag (blood component bag) formed by fusing a soft sheet material and forming it into a bag shape in the same manner as described above.
It is composed of

The blood collected in the container 6 is centrifuged to separate it into a plurality of blood components, and the container 6 is pressed, whereby the upper blood component (eg, plasma) of the separated components is pressed. Is transferred to the container 9 via the tube 10 and separated.

It should be noted that, different from the illustration, a plurality of containers (blood component bags) 9 may be connected to the container 6 in series or in parallel with the container 6.

Thus, for example, platelet-rich and platelet-poor plasma can be fractionated into separate containers (blood component bags) 9 respectively, and after cooling the container 9 containing the plasma, it is centrifuged and centrifuged. Tate is obtained as a sediment and used for separation of plasma components, such as collecting the removed plasma in another container 9, or washing the concentrated red blood cells once collected using the container 9, and collecting the washing solution. Another container 9
Various usage modes are possible, such as using.

The blood processing device 1 shown in FIG. 6 has a container 11 for storing a red blood cell preserving solution, the container 11 and the second tube 5.
And a tube 12 for connecting Other configurations are the same as those of the blood processing device 1 shown in FIG.

The container 11 is formed of a bag (red blood cell preservation liquid bag) formed by fusing a soft sheet material and forming the bag into a bag shape in the same manner as described above.

Examples of the erythrocyte preservation solution contained in the container 11 include a physiological solution containing one or more of adenine, mannitol, sorbitol, guanosine and the like. Specifically, S.M. A. G. FIG. M. Liquid, Optisol liquid,
A MAP solution or the like can be used.

After the blood treatment, the leukocyte-free blood in the container 6 is subjected to a centrifugal separation operation, and the upper layer of plasma is removed from the container
Then, the concentrated red blood cells remain in the container 6. A red blood cell preservation solution is added to the concentrated red blood cells from the container 11 via the tube 12 and the second tube 5. This increases the effective storage time of red blood cells.

The blood processing device 1 shown in FIG. 7 is the same as the blood processing device 1 shown in FIG. 5 except for the connection pattern of the container 11 for storing the red blood cell preserving solution. That is, the other end of the tube 12 whose one end is connected to the container 11 is directly connected to the container 6.

In the blood processing device 1 shown in FIG. 8, the third tube 8 in the blood processing device 1 shown in FIG. 5 is connected by an aseptic connection device in the same manner as the blood processing device 1 shown in FIG. The part C1 is connected aseptically for use.

The blood processing device 1 shown in FIG. 9 is similar to the blood processing device 1 shown in FIG. 6 except that the third tube 8, the second tube 5 and the tube 12 are connected aseptically in the same manner as described above. It is designed to be used aseptically by connecting devices at connection portions C1, C2, and C3.

In the blood processing device 1 shown in FIG. 10, the third tube 8 and the tube 12 in the blood processing device 1 having the structure shown in FIG. It is designed to be used aseptically connected at C4.

The blood processing device 1 shown in FIGS. 8 to 10 can also be manufactured in the same manner as described above by changing the tubes.
Packaging, transportation and storage benefits.

The blood processing device 1 shown in FIG. 11 is the same as the blood processing device 1 shown in FIG. 5 except that the container 6 and the container 9 are connected in parallel. That is, the other end of the tube 10 whose one end is connected to the container 9 is connected in the middle of the second tube 5.

The blood processing device 1 shown in FIG. 12 is used by aseptically connecting the tube 10 to the blood processing device 1 having the structure shown in FIG. It is like that.

FIG. 13 is a plan view showing the structure of the cell removing filter (white blood cell removing filter) 4, and FIG. 14 is a sectional view taken along line AA in FIG. This cell removal filter 4
A flat casing 41 having a blood inlet 42 and an outlet 43 formed therein, and a filter medium (filter member) arranged so as to isolate the internal space of the casing 41 between the inlet 42 and the outlet 43 side. ) 44.

The first tube 3 is connected to the inlet 42 and the second tube 5 is connected to the outlet 43 in a liquid-tight manner.

The filter medium 44 has a function of capturing leukocytes in blood, but substantially passing other blood components, such as plasma, red blood cells, and platelets. This filter medium 4
Examples of the constituent material 4 include a fibrous substance, a porous substance, a particulate substance, and the like, and the material is preferably a hydrophilic material. This is because if it is hydrophobic, the penetration of blood will not proceed smoothly.

In the illustrated configuration, the filter medium 44 is formed by laminating a plurality of porous membranes. Examples of the porous membrane include a porous body made of polyurethane, a porous body made of polyvinyl alcohol, and a porous body made of polypropylene. In the case of polypropylene, a hydrophilic treatment such as a surfactant coat is performed.

The average pore size of the porous membrane is preferably 3 to 5
The thickness is about 0 μm, more preferably 5 to 30 μm.

Such a leukocyte removal filter has a capacity of 400
When the blood of ml is processed, it preferably has a performance such that the number of white blood cells in the processed blood is less than 1 × 10 ⁸ , particularly a performance such that the number of leukocytes is 1 × 10 ⁷ or less. Thereby, the leukocyte removal rate in the processed blood collected in the container 6 is increased, and the efficacy and safety as a blood product are improved.

In the leukocyte removal filter of the present invention,
To improve the leukocyte capture efficiency (removal rate), filter medium 4
Surface treatment such as coating the surface of the porous membrane constituting 4 with a leukocyte-capturing polymer material can also be performed.

As a coating agent for performing such a surface treatment, for example, a coating agent having a basic nitrogen-containing functional group, such as a copolymer of diethylaminoethyl (meth) acrylate and hydroxyethyl (meth) acrylate, may be used. Molecular materials are preferably used.

It should be noted that the porous material made of polyurethane is
In the case where the platelet is used as No. 4 and the platelet is intended to be passed without being removed, such a surface treatment is not performed because platelet adhesion is enhanced.

The blood processing apparatus of the present invention includes a flow detecting means 30 for detecting the flow rate of blood flowing through the first tube 3 or the second tube 5 in any one of the blood processing tools 1 described above; This is a combination of the control means 25 for controlling the supply amount of the anticoagulant liquid from the anticoagulant container 7 in accordance with the flow rate detected by the flow rate detection means 30. Hereinafter, those configurations will be described based on the illustrated embodiment.

FIGS. 15 and 16 are a perspective view and a block diagram, respectively, showing the appearance of an embodiment of the blood processing apparatus of the present invention, and FIG. 17 is a front view of the main body of the blood processing apparatus in the blood processing apparatus.

As shown in these figures, the blood processing apparatus 2
0 is a blood processing apparatus main body 21, a flow detecting means 30,
It has a tube clamp 31, a display section 32, and a power supply section 33.

The main body 21 of the blood processing apparatus comprises a microcomputer 22, a chuck 23, a cylinder 24 which is a driving source of the chuck 23, a power supply circuit 26, a power supply switch circuit 27, and an operation section including a key switch and the like. 28. Of these, chuck 23 and cylinder 24
Thus, a channel opening / closing mechanism for opening and closing the third channel is configured,
Further, the flow path opening / closing mechanism and the microcomputer (control unit) 22 constitute control means 25 for controlling the supply amount of the anticoagulant liquid.

The anticoagulant container 7 is suspended and supported at a high position by a stand 50. A part of the third tube 8 is loaded in the chuck 23, and its internal flow path can be opened and closed. .

The chuck 23 includes a pair of holding members 231,
232, between which the third tube 8 is inserted, and the two holding members 231 and 23
2 approach / separate and pinch / open the third tube 8. In a state where the third tube 8 is sandwiched by the chuck 23 (a state shown in FIG. 17), the internal flow path (third flow path) is shut off, and in an open state, the internal flow path is opened. State.

In this case, the supply amount of the anticoagulant supplied through the third tube 8 depends on the frequency of opening and closing the internal flow path of the third tube 8 by the operation of the chuck 23 and the duration of the open state. .

The cylinder 24 for operating the chuck 23 may be any of an electric cylinder, an air cylinder, and a hydraulic cylinder, but is preferably an electric cylinder in terms of simplification of the device structure.

The chuck 23 is operated by a motor and a mechanism such as a crank and a cam for converting the drive of the motor into a linear motion, and the third tube 8 is opened and closed. It can also be.

The flow rate detecting means 30 is mounted on the first tube 3 on the upstream side of the branch portion 13. The flow rate detection means 30 is composed of various flow meters (flow rate sensors) such as an electromagnetic flow meter and an ultrasonic Doppler flow meter, and preferably measures the flow rate of blood flowing through the first tube 3. Can be detected in real time (as needed).

Further, it is preferable that the flow rate detecting means 30 can detect the integrated flow rate. This allows
There is an advantage that the end of the blood processing (blood collection), that is, the completion of the target blood collection amount can be automatically detected.

The flow rate detecting means 30 and the microcomputer 22 are connected by a cable 38, and data detected by the flow rate detecting means 30 is inputted to the microcomputer 22 at any time or at an appropriate time.

The tube clamp 31 opens and closes the internal flow path of the first tube 3 and is installed near the flow detecting means 30. As the tube clamp 31, one having the same configuration as the chuck 23 and the cylinder 24 can be used. The tube clamp 31 is opened at the start of the blood treatment, and at the end of the blood treatment, that is, the target blood collection volume (for example, 200 ml, 400 m
l) On arrival, it is closed.

The operation unit 28 has a power switch 281, a blood collection amount setting switch 282, a start / stop switch 283, and a fast forward switch 284. The fast-forward switch 284 is used to quickly feed the anticoagulant with the chuck 23 opened before priming the blood, and to prime the third tube 8 with the anticoagulant.

The display section 32 is, for example, a light emitting element (LED)
And a liquid crystal display.
Information such as N, OFF, setting conditions of the device, operating state of the device, blood flow rate, abnormal information, and drip operation completion display can be displayed. In the illustrated configuration, each of the switches 281 to 281
By turning on / off a light emitting element (LED) built in the 284, information such as power ON / OFF, set value of blood collection amount, operation state of the apparatus, and the like are displayed.

The power supply section 33 includes an AC power connector 34,
It has a transformer 35 and a rectifier circuit 36. The power supply unit 33 and the power supply circuit 26 are connected by a cable 37.

According to the blood processing method of the present invention, the cell removing filter 4 is arranged on the upstream side of the container 6 via the second channel, and the cell removing filter 4 is provided on the upstream side of the cell removing filter 4 via the first channel. It has a blood supply unit (puncture needle 2), and further has a third flow path that joins the first flow path, so that blood from the blood supply part is passed through the cell removal filter 4 for processing. Then, the blood flow is detected, an appropriate amount of the anticoagulant is supplied to the upstream side of the cell removal filter 4 via the third flow path, and the blood to which the anticoagulant is added and mixed is removed. This is a method of obtaining blood from which predetermined cells such as white blood cells have been removed by passing the blood through the filter 4 and collecting the blood in the container 6.

Hereinafter, a case in which this blood processing method is performed using the blood processing apparatus 20 will be described.

If necessary, as a pre-process (preparation process),
According to the form of the blood processing device 1, necessary portions of the connection portions C1 to C4 are aseptically connected by the aseptic connection device. For example, when using the blood processing device 1 shown in FIGS. 2, 3, 4, 8, 9, and 10, the third tube 8 is connected by the above-described aseptic connection device prior to blood processing. The part C1 is connected aseptically.

As shown in FIG. 15, the container 6 is placed at a low place, the third tube 8 is set on the chuck 23, and the flow rate detecting means 30 is mounted on the first tube 3.

Next, the power switch 2 of the blood processing apparatus 20
81 is turned on, the blood collection amount setting switch 282 is used to select the blood collection amount of 200 ml or 400 ml, the fast-forward switch 284 is turned on to fill (prime) the third tube 8 with the anticoagulant solution, and the start / stop switch At 283, the start of the process is set. In addition, the third tube 8
At the time of priming inside, the breaking communication member is broken to open the internal flow path.

Next, when the puncture needle 2 is punctured into the blood vessel (vein) of the donor and the tube clamp 31 is opened, the blood collected flows through the first tube 3 due to the venous pressure and the head. It is introduced into the cell removal filter 4 through the inlet 42. When passing through the filter medium 44 of the cell removal filter 4, white blood cells (or white blood cells and platelets) in the blood are adsorbed and removed by the filter medium 44, and the blood component excluding the white blood cells flows out of the outlet 43 of the cell removal filter 4. Then, it is introduced into the container 6 through the second tube 5 and collected.

At this time, an appropriate amount of the anticoagulant solution is supplied and mixed with the blood flowing through the first tube 3.
Blood can be prevented from coagulating before flowing into the cell removal filter 4.

That is, the flow rate detecting means 30 detects the flow rate of the blood flowing through the first tube 3 in real time, and the detected value is input to the microcomputer 22 to perform predetermined data processing (arithmetic processing). Is made. Then, the microcomputer 22 sets an appropriate supply amount of the anticoagulant solution according to the blood flow rate, controls the operation of the cylinder 24, and opens and closes the chuck 23 in a predetermined pattern in order to execute the setting.

An example of the relationship between the blood flow and the opening / closing pattern of the chuck 23 is shown in Table 1 below. The data in Table 1 is stored in advance in a memory built in the microcomputer 22, and a chuck opening / closing pattern corresponding to the detected blood flow is set and executed based on the data.

The opening / closing pattern of the chuck 23 can be set based on a set head, a standard blood collection time, and the like. Further, the supply amount (supply speed) of the anticoagulant liquid may be increased or decreased depending on the situation.

[0093]

[Table 1]

By controlling the supply amount of the anticoagulant liquid as described above, the blood is continuously or via the third tube 8 from the anticoagulant container 7 before passing through the cell removing filter 4. It is mixed with an appropriate amount of an anticoagulant liquid supplied intermittently. Thereby, regardless of the fluctuation of the blood flow, the anticoagulant effect is exerted on the blood without excess or deficiency. Therefore, the clogging of the filter medium 44 by the blood coagulation is effectively prevented and the blood is collected in the container 6. No excess anticoagulant is added to the processed blood.

When the blood processing tool 1 shown in FIGS. 1, 2 and 5 to 12 is used, the chuck 23 is opened and left in the anticoagulant container 7 before and after the end of the blood processing. All of the anticoagulant liquid may be supplied.

After the blood treatment (blood collection) is completed as described above, the middle of the second tube 5 is pressure-bonded with, for example, an aluminum ring, or heat-sealed with a heat sealer, and cut to form a container 6. , Blood that has been processed (leukocyte-depleted blood or leukocyte-platelet-depleted blood) is obtained.

In the case where the blood processing tool 1 shown in FIGS. 5, 6, 8, and 11 is used, which has the container 6 attached to the container 6, after the blood processing is completed, To
Sealing and cutting are performed in the same manner as described above, and the connected body of the container 6 and the container 9 is subjected to centrifugal separation to separate the blood in the container 6 into a plurality of blood components (plasma in the upper layer, and dense red blood cells from which leukocytes are removed in the lower layer). Then, the upper plasma component is transferred to the container 9 via the tube 10 and collected. Thereafter, if the tube 10 is sealed and cut in the same manner as described above, the container 6 is obtained aseptically with the leukocyte-removed concentrated red blood cells and the container 9 is obtained aseptically.

In this case, since the centrifugal separation operation may be performed on the connected body of the containers 6 and 9, the operability is excellent, and the casing 41 of the cell removal filter 4 may be damaged, or the containers 6 and 9 may be damaged. There is no inconvenience such as being crushed by the casing 41 of the cell removing filter 4, and the safety is high.

When using the blood processing tool 1 with the container 11 shown in FIGS. 9 and 10, after performing the blood processing, the leukocyte-depleted blood in the container 6 is subjected to a centrifugal separation operation. The upper layer of plasma is transferred to the container 9, and a red blood cell storage solution is added from the container 11 to the leukocyte-removed concentrated red blood cells remaining in the container 6. As a result, the effective storage time of red blood cells in the container 6 becomes longer.

A new container 6 or a connected body of the container 6 and the container 9 is prepared. The tube (the end is sealed) connected to the container 6 and the second container connected to the cell removing filter 4 are prepared. The second tube 5 (the end of which is sealed) can be aseptically connected by the aseptic connection device to enable the next blood treatment. This operation may be performed as a pre-process (preparation process) before performing the blood treatment.

In the above method, the blood processing method is basically the same even when the blood processing apparatus 20 'described below is used.

FIGS. 18 and 19 are a perspective view and a block diagram, respectively, showing the appearance of another embodiment of the blood processing apparatus of the present invention. FIG. 20 is a front view of the main body of the blood processing apparatus in the blood processing apparatus. is there.

The blood processing apparatus 20 'shown in these figures
15 to 17 except that the configuration of the control means for controlling the supply amount of the anticoagulant liquid is different.
Is the same as Hereinafter, the differences will be described.

The control means 25 in the blood processing apparatus 20 ′ comprises a roller pump 29 as a pump for feeding the anticoagulant solution, and a microcomputer (control section) 22 for controlling the discharge amount of the roller pump 29. It is configured.

As shown in FIG. 20, the roller pump 29
Has a plurality of rollers 291, and each roller 291 rotates and revolves to perform a squeezing operation while pressing and closing the third tube 8 with the arc-shaped guide member 292.

As a result, a flow of the anticoagulant is formed in the third tube 8, and the anticoagulant is supplied from the branch portion 13 into the first tube 3 and mixed with the blood flowing therethrough. . In this case, the magnitude of the revolution speed of each roller 291 corresponds to the magnitude of the discharge amount of the roller pump 29, that is, the magnitude of the supply amount of the anticoagulant liquid.

A support plate 293 made of a transparent plate is attached to the front of the roller pump 29 by fixing members 294 at its four corners. Support plate 293
Is transparent, it is possible to easily confirm the state where the third tube 8 is closed by the roller 291 under pressure.

The flow rate detecting means 30 detects the flow rate of the blood flowing through the first tube 3 in real time, and the detected value is input to the microcomputer 22 to perform predetermined data processing (arithmetic processing). You. Then, the microcomputer 22 sets an appropriate supply amount of the anticoagulant liquid according to the blood flow rate, and controls the rotation speed of the roller pump 29 (revolution speed of the roller 291) in order to execute the setting.

As described above, in the present invention, when treating blood, an appropriate amount of an anticoagulant is added to the blood as needed.
It is then passed through a cell removal filter shortly. Thereby, while the anticoagulant solution is added in advance and the blood left for a certain period of time is processed (filtered), the recovery rate of red blood cells is improved.

According to the study of the present inventor, when the blood added with the anticoagulant solution is stored before being processed (filtered),
It was found that the longer the storage time, the lower the recovery of red blood cells in the treated blood. This is because ATP (adenosine triphosphate) or PEP
(Phosphoenolpyruvate) causes a sharp drop in energy carriers, resulting in red blood cell deformability,
That is, it is assumed that the function of passing through the pores of the filter medium 44 is impaired.

On the other hand, in the present invention, the addition and mixing of the anticoagulant and the treatment with the cell removing filter 4 are performed almost simultaneously, so that after the anticoagulant solution is added to the blood as described above, There is no storage time until processing (filtration). Therefore, there is no decrease in the deformability of the red blood cells, and the recovery rate of the red blood cells is improved.

Although the blood processing method and the blood processing apparatus of the present invention have been described based on the embodiments shown in the accompanying drawings, the present invention is not limited to these. In particular, the configuration of the blood processing device 1 may be any configuration other than those shown in the drawings, for example, the anticoagulant liquid flows backward in the first tube 3 (first flow path). Then, in order to prevent the blood from being injected into the blood vessel of the blood donor, a non-return that allows only the flow toward the cell removal filter 4 between the puncture needle 2 and the branch portion 13 of the first tube 3 A configuration in which a backflow prevention mechanism (not shown) such as a valve may be provided.

The method of introducing the treated blood into the container 6 is not limited to the method using a head (gravity) as described above, and may be, for example, a vacuum blood collection device (manufactured by Terumo, trade name: Hemoquick). 6 (and the container 9), and a method of keeping the container 6 under reduced pressure. A method of sucking and introducing into the container 6 may be adopted.

The pump for supplying the anticoagulant liquid is not limited to the roller pump described above.
Various pumps such as a syringe pump and an impeller pump can be used.

[0115]

【Example】

(Example 1)

1) Outline of Apparatus A blood processing apparatus having the structure shown in FIG. 5 was used.

As the cell removing filter 4, a leukocyte removing filter having the structure shown in FIG. 13 and FIG. 14 (filter material: two-layer laminate of polyurethane porous membrane, average pore diameter 10 μm)
Was used.

The capacity of the container 6 was 400 ml for collecting blood, and the capacity of the container 9 was 300 ml. These containers 6 and 9 are each made of soft polyvinyl chloride (plasticizer: DEHP 31.
(A 7 wt% blend) sheet material (thickness 0.39 mm) was formed into a bag.

The anticoagulant container 7 is made of the same soft polyvinyl chloride bag as described above, and has a capacity of 10 bags.
It was 0 ml. In this anticoagulant bag, 56 ml of CPD liquid was put as an anticoagulant liquid.

The first tube 3, the second tube 5, the third tube 8 and the tube 10 are all made of soft polyvinyl chloride (plasticizer: 36.7% by weight of DEHP).
The outer diameter was 4.4 mm and the inner diameter was 3.0 mm.

The transfer of the blood during the blood treatment is performed in the container 6.
Was carried out due to a head drop by placing the camera in a low place.

The control of the supply amount of the anticoagulant is shown in FIG.
17 was performed using the blood processing apparatus 20 shown in FIG. Specifically, the supply amount of the anticoagulant was controlled by the opening / closing pattern of the chuck whose opening / closing operation was controlled by the microcomputer.

The blood flow was detected by using an electromagnetic flowmeter, determining the opening / closing pattern of the chuck based on the detected data based on Table 1, and executing this.

2) Operation procedure

The power supply unit 33 was connected to an AC power supply (100 V AC), and the power switch 281 was turned on.

The third connected to the anticoagulant container 7
The tube 8 was mounted on the chuck 23. Further, a flow rate detecting means (flow rate sensor) 30 was attached to the first tube 3.

The blood collection amount setting switch 282 was used to select a blood collection amount of 400 ml.

The breaking communication member (trade name: Click Tip manufactured by Terumo Corporation) installed in the third tube 8 is broken, the fast-forward switch 284 is pressed to open the chuck 23, and the anticoagulant liquid is branched. Then, the inside of the third tube 8 was primed with the anticoagulant solution, and the chuck 23 was closed there to stop the anticoagulant solution.

[0130] With the tube clamp 31 opened, the puncture needle 2 was punctured into the vein of the donor, and blood collection was started. The initial flow of blood flows through the first tube 3 and the branch 1
At the same time as passing through No. 3, the start / stop switch was pressed to start the operation, and the supply of the anticoagulant was started.

At this time, the flow rate of the blood flowing in the first tube 3 fluctuated, but the supply amount of the anticoagulant solution (CPD solution) was controlled to an appropriate amount accordingly.

The blood to which an appropriate amount of the anticoagulant solution has been added and mixed passes through a leukocyte removal filter, in which a part of leukocytes and platelets is removed. 5 and introduced into the container 6.

When the blood flow detection means 30 detects that the blood collection amount (integrated flow rate) has reached 400 ml, the tube clamp 31 is closed, the internal flow path of the first tube 3 is shut off, and the blood processing (blood collection) is started. Stopped.

When the blood processing (blood collection) is completed, the puncture needle 2 is withdrawn from the blood donor's vein, the first tube 8 near the puncture needle 2 is closed with a clamp, and then the fast-forward switch 284 is pressed again to prevent anticoagulation. Almost all of the anticoagulant solution (about 2-3 ml) remaining in the agent container 7 was discharged and added to the treated blood collected in the container 6.

Thereafter, the power switch 281 is turned off.
Then, the blood processing tool 1 was removed from the blood processing apparatus. The time required for such a blood treatment (from the start of blood collection to the end of the addition of the remaining amount of the anticoagulant solution) was about 10 minutes.

Thereafter, the following post-processing (post-process)
Was done. The middle of the second tube 5 was heat-sealed with a tube sealer, and the sealed portion was cut, so that leukocyte-platelet-free blood was obtained in the container 6.

Next, the combined body of the containers 6 and 9 was set in a centrifuge at 4 ° C., and centrifuged at 3000 G for 6 minutes. Thereafter, the containers 6 and 9 were taken out of the centrifuge, the container 6 was pressed using a separation stand, and the upper layer (supernatant) plasma was transferred to the container 9 via the tube 10.

Thereafter, the tube 10 was heat-sealed with a tube sealer, and the sealed portion was cut to obtain a container 6 containing concentrated red blood cells and a container 9 containing plasma.

Blood collection-blood processing (filtration)-
The centrifugation-blood component measurement was performed at the timing shown in FIG.

[Measurement of Blood Component] In order to measure the initial blood properties of a blood donor, 10 ml of blood was collected using a vacuum blood collection tube containing a CPD solution, and the number of red blood cells, the number of white blood cells, etc. were measured.
Then, these were compared with the concentrated red blood cells obtained in the container 6, and the white blood cell residual rate and the red blood cell recovery rate were obtained.
The amount of plasma collected in the container 9 was also measured. The results are shown in Table 2 below.

(Comparative Examples 1 to 3) 400 ml of blood collected from a donor was placed in a flexible polyvinyl chloride blood collection bag.
Here, 56 ml of CPD solution was added and mixed as an anticoagulant solution. After a lapse of a predetermined time, the blood with the anticoagulant was passed through the same leukocyte removal filter as in Example 1, and the leukocyte-free blood was collected in one of the containers connected to the containers 6 and 9.

Thereafter, centrifugation was carried out in the same manner as in Example 1 to obtain a container containing concentrated red blood cells and a container containing plasma.

Blood collection, blood processing (filtration), centrifugation, and blood component measurement were performed at the timing shown in FIG.

It is to be noted that 2 hours after blood collection (Comparative Example 1) is assumed to be a standard time at room temperature until a container (blood collection bag) storing collected blood is delivered from the blood collection bus to the blood center. Things.

For Comparative Examples 1 to 3, blood components were measured in the same manner as described above. The results are shown in Table 2 below.

[0146]

[Table 2]

(Example 2)

1) Outline of Apparatus A blood processing apparatus having the structure shown in FIG. 8 was used.

Cell removal filter 4, containers 6, 7, 9, first tube 3, second tube 5, third tube 8
The tube 10 and the tube 10 had the same conditions as in Example 1. However, the capacity of each of the containers 6, 7, and 9 was half that of Example 1, and 28 ml of a CPD solution was placed in the container 7 as an anticoagulant solution.

[0150] The transfer of blood during blood processing
Vacuum blood sampling device (Termo Corp., trade name: Hemoquick)
Was carried out by putting into a vacuum chamber.

The control of the supply amount of the anticoagulant is performed according to FIG.
20 using a blood processing apparatus 20 'shown in FIG. Specifically, the microcomputer controlled the number of revolutions of the roller pump, that is, the discharge amount, to control the supply amount of the anticoagulant. The blood flow was detected using an electromagnetic flowmeter.

2) Operation procedure

The power supply unit 33 was connected to an AC power supply (100 V AC), and the power switch 281 was turned on.

Using the aseptic connection device described in Japanese Utility Model Application Laid-Open No. 6-268877, the third tube 8a on the anticoagulant container 7 side and the third tube 8b on the branch portion 13 side are connected at the connection portion C1. This was connected to form a third tube 8.

The connected third tube 8 was mounted on the roller pump 29. Further, a flow rate detecting means (flow rate sensor) 30 was attached to the first tube 3.

The blood collection volume setting switch 282 was used to select a blood collection volume of 200 ml.

The break communication member (trade name: Click Tip manufactured by Terumo Corporation) installed in the third tube 8 is broken, the fast-forward switch 284 is pressed to open the chuck 23, and the anticoagulant liquid is branched. Then, the inside of the third tube 8 was primed with the anticoagulant solution, and the chuck 23 was closed there to stop the anticoagulant solution.

With the tube clamp 31 opened, the puncture needle 2 was punctured into the vein of the donor, and blood collection was started. The initial flow of blood flows through the first tube 3 and the branch 1
At the same time as passing through No. 3, the start / stop switch was pressed to set the start state, and the supply of the anticoagulant solution was started.

At this time, the flow rate of the blood flowing through the first tube 3 fluctuated, but the supply amount of the anticoagulant solution (CPD solution) was controlled to an appropriate amount accordingly.

The blood to which an appropriate amount of the anticoagulant solution has been added and mixed passes through a leukocyte removal filter, in which a part of leukocytes and platelets is removed, and the leukocyte / platelet-depleted blood is passed through a second tube. 5 and introduced into the container 6.

When the flow rate detecting means 30 detects that the blood collection amount (integrated flow rate) has reached 200 ml, the tube clamp 31 is closed, the internal flow path of the first tube 3 is shut off, and the blood processing (blood collection) is started. Stopped.

After the blood treatment (blood collection) is completed, the puncture needle 2 is withdrawn from the blood donor's vein, the first tube 8 near the puncture needle 2 is closed with a clamp, and the fast-forward switch 284 is pressed again to prevent anticoagulation. Almost all of the anticoagulant solution (about 2-3 ml) remaining in the agent container 7 was discharged and added to the treated blood collected in the container 6.

After that, the power switch 281 is turned off.
Then, the blood processing tool 1 was removed from the blood processing apparatus. The time required for such a blood treatment (from the start of blood collection to the end of the addition of the remaining amount of the anticoagulant solution) was about 10 minutes.

'Thereafter, the following post-processing (post-process) was performed. The middle of the second tube 5 was heat-sealed with a tube sealer, and the sealed portion was cut, so that leukocyte-platelet-free blood was obtained in the container 6.

Next, the combined body of the containers 6 and 9 was set in a centrifuge at 4 ° C., and centrifuged at 3000 G for 6 minutes. Thereafter, the containers 6 and 9 were taken out of the centrifuge, the container 6 was pressed using a separation stand, and the upper layer (supernatant) plasma was transferred to the container 9 via the tube 10.

Thereafter, the tube 10 was heat-sealed with a tube sealer, and the sealed portion was cut to obtain a container 6 containing concentrated red blood cells and a container 9 containing plasma.

Further, in order to perform the next blood treatment, the connected body of the containers 6 and 9 was connected at the connection portion C2 using the aseptic connection device.

Blood collection-blood processing (filtration)-
The centrifugation-blood component measurement was performed at the timing shown in FIG.

[Measurement of blood components] The amount of collected plasma, the residual ratio of white blood cells, and the recovery of red blood cells were determined in the same manner as described above. The results are shown in Table 3 below.

(Comparative Examples 4 to 6) 200 ml of blood collected from a donor was placed in a flexible polyvinyl chloride blood collection bag.
Here, 28 ml of CPD solution was added as an anticoagulant solution and mixed. After a lapse of a predetermined time, the blood with the anticoagulant was passed through the same leukocyte removal filter as in Example 1, and the leukocyte-free blood was collected in one of the containers connected to the containers 6 and 9.

Thereafter, centrifugation was performed in the same manner as in Example 2 to obtain a container containing concentrated red blood cells and a container containing plasma.

[0172] Blood collection, blood processing (filtration), centrifugation, and blood component measurement were each performed at the timing shown in FIG.

It is assumed that 2 hours after the blood collection (Comparative Example 4) is a standard time at room temperature until the container (blood collection bag) storing the collected blood is delivered from the blood collection bus to the blood center. Things.

For Comparative Examples 4 to 6, blood components were measured in the same manner as described above. The results are shown in Table 3 below.

[0175]

[Table 3]

[Consideration of Measurement Results] As shown in Tables 2 and 3, in Examples 1 and 2, about 90% of the red blood cells collected from the donor were collected in the container 6 and the mixed white blood cells were collected. Showed an extremely high leukocyte removal rate of about 0.3% of leukocytes collected from a donor. In addition, 181 ml of plasma could be collected in the container 9. In addition, during blood processing, troubles such as blood coagulation (thrombus formation) did not occur in the leukocyte removal filter and the like, and no damage occurred to the containers 6 and 9 during the centrifugation operation.

On the other hand, in Comparative Examples 1 to 6, it was confirmed that the longer the time from blood collection (addition of an anticoagulant) to filtration, the red blood cell recovery rate tends to decrease.

[0178]

As described above, according to the present invention, blood can be rapidly processed by a simple operation, particularly a series of operations, and the anticoagulant solution can be treated in accordance with the blood flow rate. By controlling the supply amount, an appropriate amount of the anticoagulant solution can be supplied in real time, and the anticoagulant function can be effectively exhibited without excess or deficiency.

In particular, when the present invention is applied to a process for removing at least white blood cells and recovering red blood cells, the time between the addition of the anticoagulant solution to the blood and the passage through the cell removal filter is short. The recovery rate is improved.

Further, by removing leukocytes, particularly at a high removal rate, the occurrence of microaggregates in the collected leukocyte-depleted blood (red blood cell preparation) and the occurrence of hemolysis are reduced.

When the second flow path and the third flow path are aseptically connected, production, transportation, storage, and the like can be performed on the entire blood processing device in a state where its bulk is reduced, and operability is improved. In addition to improving the cost and reducing the cost, when performing centrifugation, it can be used for centrifugation with the unnecessary cell removal filter etc. removed. Increase.

[Brief description of the drawings]

FIG. 1 is a schematic diagram showing a configuration example of a blood processing tool according to the present invention.

FIG. 2 is a schematic diagram illustrating a configuration example of a blood processing device according to the present invention.

FIG. 3 is a schematic diagram illustrating a configuration example of a blood processing device according to the present invention.

FIG. 4 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 5 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 6 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 7 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 8 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 9 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 10 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 11 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 12 is a schematic diagram illustrating a configuration example of a blood processing tool according to the present invention.

FIG. 13 is a plan view showing a configuration of a cell removal filter (white blood cell removal filter) according to the present invention.

FIG. 14 is a sectional view taken along line AA in FIG.

FIG. 15 is a perspective view showing the appearance of an embodiment of the blood processing apparatus of the present invention.

16 is a block diagram showing a circuit configuration of the blood processing apparatus shown in FIG.

17 is a front view of the blood processing apparatus main body in the blood processing apparatus shown in FIG.

FIG. 18 is a perspective view showing the appearance of an embodiment of the blood processing apparatus of the present invention.

19 is a block diagram showing a circuit configuration of the blood processing apparatus shown in FIG.

20 is a front view of the blood processing apparatus main body in the blood processing apparatus shown in FIG.

FIG. 21 is a timing chart showing processing steps in the example.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 blood processing tool 2 puncture needle 3 first tube 4 cell removal filter 41 casing 42 inflow port 43 outflow port 44 filter medium 5 second tube 6 container 7 anticoagulant container 8 third tube 9 container 10 tube 11 container 12 Tube 13 branch section 20, 20 'blood processing apparatus 21 blood processing apparatus main body 22 microcomputer 23 chuck 231, 232 holding member 24 cylinder 25 control means 26 power supply circuit 27 power switch circuit 28 operation section 281 power switch 282 blood sampling amount setting switch 283 Start / Stop switch 284 Fast-forward switch 29 Roller pump 30 Blood flow detecting means 31 Tube clamp 32 Display unit 33 Power supply unit 34 AC power connector 35 Transformer 36 Rectifier circuit 37, 38 Cable 50 Stand C1-C4 connection Part

Claims (12)

[Claims] 1. A container for storing processed blood, a cell removal filter for selectively removing predetermined cells in blood, a first flow path for introducing blood into the cell removal filter, and the cells. A second flow path that introduces the blood that has passed through the removal filter into the container, a third flow path that communicates with the first flow path, and that supplies an anticoagulant solution; It has a flow rate detecting means for detecting a flow rate of blood flowing through the second flow path, and a control means for controlling a supply amount of the anticoagulant liquid according to the flow rate detected by the flow rate detecting means. Blood processing equipment. 2. The blood processing apparatus according to claim 1, further comprising an anticoagulant container that communicates with an upstream side of the third flow path and stores the anticoagulant liquid. 3. The control unit according to claim 1, wherein the control unit includes a flow path opening / closing mechanism that opens and closes the third flow path, and a control unit that controls an operation of the flow path opening / closing mechanism. Blood processing equipment. 4. The blood processing apparatus according to claim 1, wherein the control means includes a pump for feeding an anticoagulant solution, and a control unit for controlling a discharge amount of the pump. 5. The blood processing apparatus according to claim 4, wherein the pump is a roller pump. 6. The blood processing apparatus according to claim 1, wherein the cell removal filter is a leukocyte removal filter capable of filtering at least white blood cells. 7. When the leukocyte removal filter processes 400 ml of blood, the white blood cell count in the processed blood is 1 ×.
7. The blood processing apparatus according to claim 6, wherein the blood processing apparatus has a performance of less than 10 < ⁸ >. 8. Passing the blood through a cell removal filter,
A blood processing method for removing specific cells in the blood and collecting the blood from which the cells have been removed in a container, wherein during the processing of the blood, a flow rate of the blood to be processed is detected, and the blood flow rate is determined. A blood processing method, comprising supplying a predetermined amount of an anticoagulant solution to an upstream side of the cell removal filter. 9. A container for storing processed blood, a cell removal filter for selectively removing predetermined cells in blood, a first flow path for introducing blood into the cell removal filter, and the cells. A second flow path for introducing blood passing through the removal filter into the container, and a blood processing tool having a third flow path communicating with the first flow path and supplying an anticoagulant solution, After the blood introduced from the first flow path is passed through the cell removal filter to selectively remove predetermined cells in the blood, the blood from which the cells have been removed is passed through the second flow path. When performing the blood treatment to be collected in the container via the, the flow rate of the blood flowing through the first flow path or the second flow path is detected, and the anticoagulant liquid in an amount corresponding to the flow rate is detected in the second flow path. Blood supplied through the channel 3 and before being processed by the cell removal filter And a blood processing method. 10. The blood processing method according to claim 9, wherein the blood processing tool has an anticoagulant container that communicates with an upstream side of the third flow path and stores the anticoagulant liquid. 11. The method according to claim 1, further comprising the step of aseptically connecting the anticoagulant container to the third flow path.
0. The blood processing method according to item 0. 12. The method according to claim 1, wherein the second step
The blood processing method according to claim 9, further comprising a step of aseptically connecting the container to the flow path.