JPH09510613A - 新しい突然変異ウイルス、抗ウイルス性化合物、および新しいワクチン作成方法 - Google Patents
新しい突然変異ウイルス、抗ウイルス性化合物、および新しいワクチン作成方法Info
- Publication number
- JPH09510613A JPH09510613A JP7524806A JP52480695A JPH09510613A JP H09510613 A JPH09510613 A JP H09510613A JP 7524806 A JP7524806 A JP 7524806A JP 52480695 A JP52480695 A JP 52480695A JP H09510613 A JPH09510613 A JP H09510613A
- Authority
- JP
- Japan
- Prior art keywords
- virus
- mutated
- plasmid
- cell
- thiol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.ワクチンの調製での使用に適した、非感染性で突然変異されたアルファウイ ルスにおいて、該ウイルスが保持されているシステイン・アミノ酸を別のアミノ 酸で置換することによって突然変異され、該システインがチオール−リダクター ゼ/蛋白質ジスルフィド・イソメラーゼ活性に関与している上記ウイルス蛋白質 の領域内に存在していることを特徴とするアルファウイルス。 2.(取り消し) 3.(取り消し) 4.アルファウイルスの突然変異体が突然変異されたSindbisウイルスである請 求項1の組成物。 5.突然変異されたSindbisウイルスが突然変異されたE1−SER62である請 求項1の組成物。 6.非感染性の突然変異されたウイルスおよび上記細胞内の該DNAの表現に必 要な規制要素をコード表現する組換えDNAプラスミドにおいて、該ウイルスが 保持されているシステイン・アミノ酸を別のアミノ酸で置換することによって突 然変異され、該保持されたシステインがチオール−リダクターゼ/蛋白質ジスル フィド・イソメラーゼ活性に関与している上記ウイルス蛋白質の領域内に存在し ていることを特徴としている組換えDNAプラスミド。 7.細胞が哺乳動物の細胞である請求項6のプラスミド。 8.イースト菌がサッカロミセス、ピキア、およびクルイベロミセスで構成され るグループから選択される請求項7のプラスミド。 9.バクテリア細胞が大腸菌、バシラス・サブチリス、サルモネラ・チフィムリ ウム、シュードモナス、ストレプトミセス、およびスタフィロコッカスで構成さ れるグループから選択される請求項7のプラスミド。 10.哺乳動物細胞がBHK−21,HeLa,CHOおよびVeroで構成されるグル ープから選択される請求項7のプラスミド。 11.突然変異されたウイルスが、アルファウイルスおよびコロナ・ウイルスで構 成されるグループから選択されるウイルスの突然変異体である請求項6のプラス ミド。 12.アルファウイルスの突然変異体が突然変異されたSindbisウイルスである請 求項11のプラスミド。 13.突然変異されたSindbisウイルスが突然変異体E1−SER62である請求項 7のプラスミド。 14.請求項6のプラスミドで構成されるトランスフェクションされた細胞。 15.請求項13のプラスミドで構成されるトランスフェクションされた細胞。 16.請求項14のトランスフェクションされた細胞によってつくりだされた非感染 性の突然変異されたウイルス。 17.請求項15のラランスフェクションされた細胞によって つくりだされた非感染性の突然変異されたウイルス。 18.ウイルスを、該ウイルス内のチオール−リダクターゼ/蛋白質ジスルフィド ・イソメラーゼ活性を抑止する化合物と接触させるステップを含む、ウイルスの 感染の拡大を抑止する方法。 19.抗ウイルス剤が該ウイルス内のチオール−リダグターゼ/蛋白質ジスルフィ ド・イソメラーゼ活性を抑止することを特徴とするウイルスの感染の拡大を抑止 するための抗ウイルス剤。
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US08/213,860 US5532154A (en) | 1994-03-21 | 1994-03-21 | Mutated alpha virus |
US08/213,860 | 1994-03-21 | ||
PCT/US1995/003605 WO1995025788A1 (en) | 1994-03-21 | 1995-03-21 | Novel mutated viruses, anti-viral compounds and novel methods of making vaccines |
Publications (2)
Publication Number | Publication Date |
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JPH09510613A true JPH09510613A (ja) | 1997-10-28 |
JP3821484B2 JP3821484B2 (ja) | 2006-09-13 |
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JP52480695A Expired - Fee Related JP3821484B2 (ja) | 1994-03-21 | 1995-03-21 | 新しい突然変異ウイルス、抗ウイルス性化合物、および新しいワクチン作成方法 |
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US (1) | US5532154A (ja) |
EP (1) | EP0755440A4 (ja) |
JP (1) | JP3821484B2 (ja) |
KR (1) | KR100394163B1 (ja) |
CN (1) | CN1122105C (ja) |
AU (1) | AU688887B2 (ja) |
CA (1) | CA2186044C (ja) |
FI (1) | FI963717A (ja) |
IL (1) | IL112990A (ja) |
NZ (1) | NZ283575A (ja) |
RU (1) | RU2194754C2 (ja) |
SA (1) | SA95150618B1 (ja) |
WO (1) | WO1995025788A1 (ja) |
ZA (1) | ZA952259B (ja) |
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US6015686A (en) * | 1993-09-15 | 2000-01-18 | Chiron Viagene, Inc. | Eukaryotic layered vector initiation systems |
US6451592B1 (en) * | 1996-04-05 | 2002-09-17 | Chiron Corporation | Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis |
US6458560B1 (en) | 1996-04-05 | 2002-10-01 | Chiron Corporation | Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis |
EP0981344A1 (en) * | 1997-05-14 | 2000-03-01 | The University of New Mexico | Inhibition of cell surface protein disulfide isomerase |
US7335363B2 (en) * | 1997-09-18 | 2008-02-26 | Research Development Foundation | Membrane virus host range mutations and their uses as vaccine substrates |
US7128915B2 (en) * | 1997-09-18 | 2006-10-31 | Research Development Foundation | Membrane virus host range mutations and their uses as vaccine substrates |
CA2325564A1 (en) * | 1998-03-27 | 1999-10-07 | Cytos Biotechnology Ag | Inducible alphaviral gene expression system |
WO2000006205A1 (en) * | 1998-07-29 | 2000-02-10 | Invitrogen Corporation | Regulated expression of recombinant proteins using rna viruses |
US8647864B2 (en) * | 1999-04-14 | 2014-02-11 | Novartis Ag | Compositions and methods for generating an immune response utilizing alphavirus-based vector systems |
AU4366000A (en) * | 1999-04-14 | 2000-11-14 | Chiron Corporation | Compositions and methods for generating an immune response utilizing alphavirus-based vector systems |
US6890487B1 (en) | 1999-09-30 | 2005-05-10 | Science & Technology Corporation ©UNM | Flow cytometry for high throughput screening |
US7416903B2 (en) * | 1999-09-30 | 2008-08-26 | Stc.Unm | Wavy interface mixer |
US6498189B1 (en) * | 1999-11-10 | 2002-12-24 | University Of New Mexico | Method for induction of L-selectin shedding |
AU1647501A (en) | 1999-12-10 | 2001-06-18 | Cytos Biotechnology Ag | Replicon based activation of endogenous genes |
IES20010462A2 (en) * | 2000-05-12 | 2001-11-14 | Cyril John Higgins | Use of the world wide web |
AU2001275191A1 (en) * | 2000-05-31 | 2001-12-11 | Chiron Corporation | Method for the purification of alphavirus replicon particles |
NZ523831A (en) * | 2000-07-13 | 2005-10-28 | Invitrogen Corp | Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
EP2311994A1 (en) * | 2003-08-01 | 2011-04-20 | Life Technologies Corporation | Compositions and methods for preparing short RNA molecules and other nucleic acids |
CA2604640A1 (en) * | 2005-04-12 | 2006-10-19 | Romark Laboratories L.C. | Methods for treating diseases through interruption of protein maturation, compounds that inhibit the function of molecular chaperones such as protein disulfide isomerases or interfere with glycosylation, pharmaceutical compositions comprising them, and screening methods for identifying therapeutic agents |
WO2010042760A2 (en) * | 2008-10-08 | 2010-04-15 | The Administrators Of The Tulane Educational Fund | Compositions, methods, and kits for enhancing the immunogenicity of pathogenic antigens |
US8823943B2 (en) | 2009-05-21 | 2014-09-02 | Intellicyt Corporation | System and method for separating samples in a continuous flow |
US9752964B1 (en) | 2009-06-22 | 2017-09-05 | Stc.Unm | Flow cytometry apparatus pulling sample stream through observation chamber |
RU2720518C1 (ru) * | 2018-12-18 | 2020-04-30 | федеральное государственное бюджетное учреждение "Национальный исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи" Министерства здравоохранения Российской Федерации | Штамм вируса лихорадки Синдбис 1383 клон 3 (Sindbis fever virus 1383 clone 3) |
KR20230022150A (ko) * | 2020-03-13 | 2023-02-14 | 누오-베타 파마슈티컬 테크놀로지 (상하이) 씨오., 엘티디. | Pi4k 억제제의 항-코로나바이러스 효과 및 적용 |
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DK0500791T3 (da) * | 1989-10-31 | 1998-04-06 | Us Health | Udformning og konstruktion af ikke-infektiøse human retrovirale mutanter, der mangler genomisk DNA |
WO1994004185A2 (en) * | 1992-08-19 | 1994-03-03 | Trustees Of Boston University | Method of inhibiting reduction of disulfide bonds |
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Also Published As
Publication number | Publication date |
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EP0755440A1 (en) | 1997-01-29 |
EP0755440A4 (en) | 1998-08-19 |
CN1144536A (zh) | 1997-03-05 |
WO1995025788A1 (en) | 1995-09-28 |
KR970701778A (ko) | 1997-04-12 |
FI963717A0 (fi) | 1996-09-19 |
CA2186044C (en) | 2005-08-23 |
US5532154A (en) | 1996-07-02 |
IL112990A0 (en) | 1995-06-29 |
FI963717A (fi) | 1996-11-20 |
IL112990A (en) | 1999-11-30 |
KR100394163B1 (ko) | 2004-10-20 |
CA2186044A1 (en) | 1995-09-28 |
AU688887B2 (en) | 1998-03-19 |
JP3821484B2 (ja) | 2006-09-13 |
RU2194754C2 (ru) | 2002-12-20 |
NZ283575A (en) | 2001-03-30 |
SA95150618B1 (ar) | 2006-04-22 |
AU2191395A (en) | 1995-10-09 |
ZA952259B (en) | 1996-09-20 |
CN1122105C (zh) | 2003-09-24 |
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