JPH09500201A - 媒介物質のないグルコース測定のためのペルオキシダーゼコロイド状金オキシダーゼバイオセンサー - Google Patents
媒介物質のないグルコース測定のためのペルオキシダーゼコロイド状金オキシダーゼバイオセンサーInfo
- Publication number
- JPH09500201A JPH09500201A JP6523088A JP52308894A JPH09500201A JP H09500201 A JPH09500201 A JP H09500201A JP 6523088 A JP6523088 A JP 6523088A JP 52308894 A JP52308894 A JP 52308894A JP H09500201 A JPH09500201 A JP H09500201A
- Authority
- JP
- Japan
- Prior art keywords
- oxidase
- electrode
- colloidal gold
- hrp
- adsorbed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.以下を備えるバイオ電極: 伝導性表面; 単層または単層に近い層でコロイド状金粒子上に吸着されたペルオキシダーゼ であって、ここで該コロイド状金に吸着されたペルオキシダーゼが該伝導性表面 上に沈着され;および 該沈着コロイド状金に吸着されたペルオキシダーゼ上のオキシダーゼ層であっ て、ここで該バイオ電極が参照/対向電極と適切に組合わせられたとき、分析物 の存在下で、該伝導性表面への直接電子移動が生じる。 2.前記ペルオキシダーゼが西洋ワサビペルオキシダーゼである、請求項1に記 載のバイオ電極。 3.コロイド状金に吸着されたオキシダーゼが前記ペルオキシダーゼの上に沈着 される、請求項1に記載のバイオ電極。 4.前記オキシダーゼが、キサンチンオキシダーゼ、アミノ酸オキシダーゼ、乳 酸オキシダーゼ、アルコールオキシダーゼ、グリコレートオキシダーゼ、ピリド キサール-4-オキシダーゼ、ソルボースオキシダーゼ、グロノラクトースオキシ ダーゼまたはガラクトースオキシダーゼである、請求項1に記 載のバイオ電極。 5.前記オキシダーゼがコレステロールオキシダーゼである、請求項1に記載の バイオ電極。 6.前記オキシダーゼがグルコースオキシダーゼである、請求項1に記載のバイ オ電極。 7.前記オキシダーゼが前記電極の伝導性表面でマトリックス中に固定化される 、請求項1に記載のバイオ電極。 8.前記マトリックスが親水性ポリマーである、請求項7に記載のバイオ電極。 9.前記親水性ポリマーがカラゲナンである、請求項8に記載のバイオ電極。 10.媒介物質のないグルコースの電気化学的検出のためのバイオ電極であって 、単層で被覆されたコロイド状金に吸着された西洋ワサビペルオキシダーゼの第 1の沈着層、および該第1の層の上のコロイド状金に吸着されたグルコースオキ シダーゼの第2の沈着層を備える伝導性表面を有する、バイオ電極。 11.前記伝導性電極表面がガラス状カーボンである、請求項10に記載のバイ オ電極。 12.前記コロイド状金に吸着された西洋ワサビペルオキシダーゼの第1の層が 、前記コロイド状金に吸着されたグルコースオキシダーゼの第2の層の蒸着沈着 の前に、前記電極表面上に蒸着的に沈着される、請求項10に記載のバイオ電極 。 13.前記沈着された単層で被覆されたコロイド状金ペルオキシダーゼ層の上に 、さらに、少なくとも2種のオキシダーゼ酵素の沈着を含む、請求項1に記載の バイオ電極。 14.バイオ電極を調製するプロセスであって、伝導性表面上に、10から20の間 の、ペルオキシダーゼのコロイド状金に吸着された単層を沈着することを包含す る、プロセス。 15.請求項14に記載のプロセスにより調製される、バイオ電極。 16.請求項1に記載のバイオ電極を備える、オキシダーゼ基質の濃度を測定す るための器具。 17.分析物の酵素電気化学測定のための請求項1に記載のバイオ電極の使用。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1993/003746 WO1994024262A1 (en) | 1993-04-22 | 1993-04-22 | Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH09500201A true JPH09500201A (ja) | 1997-01-07 |
JP3204980B2 JP3204980B2 (ja) | 2001-09-04 |
Family
ID=22236527
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52308894A Expired - Fee Related JP3204980B2 (ja) | 1993-04-22 | 1993-04-22 | 媒介物質のないグルコース測定のためのペルオキシダーゼコロイド状金オキシダーゼバイオセンサー |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0695344B1 (ja) |
JP (1) | JP3204980B2 (ja) |
KR (1) | KR960701985A (ja) |
AT (1) | ATE208811T1 (ja) |
AU (1) | AU681848B2 (ja) |
CA (1) | CA2160905C (ja) |
DE (1) | DE69331150T2 (ja) |
FI (1) | FI954968A (ja) |
NO (1) | NO954216L (ja) |
WO (1) | WO1994024262A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003532897A (ja) * | 2000-05-10 | 2003-11-05 | アスラブ・エス アー | 検出成分の固定化方法 |
KR20210122979A (ko) * | 2020-04-02 | 2021-10-13 | 아주대학교산학협력단 | 세포 배양이 가능한 세포 유래물 분석용 센서칩, 및 이를 이용한 세포 활성도 정량 분석 방법 |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000511630A (ja) * | 1996-05-10 | 2000-09-05 | ビージー ピーエルシー | 酵素の直接電気化学 |
DE60025751T2 (de) | 1999-11-15 | 2006-10-12 | Arkray, Inc. | Biosensor |
US8508620B2 (en) * | 2007-02-24 | 2013-08-13 | Nec Corporation | Portable terminal capable of presenting images based on time |
US9131885B2 (en) | 2009-07-02 | 2015-09-15 | Dexcom, Inc. | Analyte sensors and methods of manufacturing same |
US9351677B2 (en) | 2009-07-02 | 2016-05-31 | Dexcom, Inc. | Analyte sensor with increased reference capacity |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01140054A (ja) * | 1987-11-26 | 1989-06-01 | Nec Corp | グルコースセンサ |
US5082550A (en) * | 1989-12-11 | 1992-01-21 | The United States Of America As Represented By The Department Of Energy | Enzyme electrochemical sensor electrode and method of making it |
US5225064A (en) * | 1992-01-15 | 1993-07-06 | Enzyme Technology Research Group, Inc. | Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination |
-
1993
- 1993-04-22 DE DE69331150T patent/DE69331150T2/de not_active Expired - Fee Related
- 1993-04-22 CA CA002160905A patent/CA2160905C/en not_active Expired - Fee Related
- 1993-04-22 EP EP93910692A patent/EP0695344B1/en not_active Expired - Lifetime
- 1993-04-22 JP JP52308894A patent/JP3204980B2/ja not_active Expired - Fee Related
- 1993-04-22 AT AT93910692T patent/ATE208811T1/de not_active IP Right Cessation
- 1993-04-22 KR KR1019950704590A patent/KR960701985A/ko not_active Application Discontinuation
- 1993-04-22 WO PCT/US1993/003746 patent/WO1994024262A1/en active IP Right Grant
- 1993-04-22 AU AU41097/93A patent/AU681848B2/en not_active Ceased
-
1995
- 1995-10-18 FI FI954968A patent/FI954968A/fi unknown
- 1995-10-20 NO NO954216A patent/NO954216L/no unknown
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003532897A (ja) * | 2000-05-10 | 2003-11-05 | アスラブ・エス アー | 検出成分の固定化方法 |
KR20210122979A (ko) * | 2020-04-02 | 2021-10-13 | 아주대학교산학협력단 | 세포 배양이 가능한 세포 유래물 분석용 센서칩, 및 이를 이용한 세포 활성도 정량 분석 방법 |
Also Published As
Publication number | Publication date |
---|---|
CA2160905A1 (en) | 1994-10-27 |
DE69331150T2 (de) | 2002-07-18 |
FI954968A0 (fi) | 1995-10-18 |
NO954216L (no) | 1995-12-14 |
EP0695344B1 (en) | 2001-11-14 |
EP0695344A1 (en) | 1996-02-07 |
NO954216D0 (no) | 1995-10-20 |
AU681848B2 (en) | 1997-09-11 |
DE69331150D1 (de) | 2001-12-20 |
ATE208811T1 (de) | 2001-11-15 |
KR960701985A (ko) | 1996-03-28 |
AU4109793A (en) | 1994-11-08 |
CA2160905C (en) | 2007-03-13 |
JP3204980B2 (ja) | 2001-09-04 |
FI954968A (fi) | 1995-12-19 |
WO1994024262A1 (en) | 1994-10-27 |
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