JPH09275975A - Biotechnological deodorant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a biotechnological deodorant useful for a fibrous product, etc., capable of directly deodorizing a bad smell caused by dirt, etc., an offensive odor in the surrounding space, by blending water or a sizing-containing solution with Bacillus subtilis Kubota having specific mycological properties. SOLUTION: Water or a sizing-containing solution is mixed with Bacillus subtilis Kubota which is a short bacillus, has 1.2 to 1.4×3.6 to 3.8μm size, is covered by a cell membrane, has a nucleus and a sporule, is observed to have a septum, has about 10 flagellums, motility and a spore, is a gram-positive and acid-fast bacterium, is inoculated into a broth agar flat plate in a floated state in a sodium chloride injection, grows well by culture at 37 deg.C for 24 hours, shows an R type in which the peripheral part of a colony will not become circular, is opaque and light yellow and is grown well by a sheep blood agar culture, heart infusion agar culture or by tryptosoya agar culture to give the objective biotechnological deodorant useful for a deodorant fibrous product.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a biodeodorant, and more specifically, to a deodorant containing Bacillus subtilis Kubota, and to which the deodorant is applied. The present invention relates to a deodorant fiber product.

[0002]

2. Description of the Related Art In recent years, a wide variety of offensive odors have been recognized along with changes in the environment and lifestyles, and people's consciousness has also changed. It is becoming more sensitive and efficient deodorant of odor,
Deodorization is required.

[0003]

SUMMARY OF THE INVENTION The present invention has been made in view of the above demands of the industry for the purpose of developing an efficient deodorizing system. However, masking a bad odor with a fragrance or the like. Different from the deodorant system based on, the focus is on a system that fundamentally decomposes and eliminates bad odors, rather than treating the odors on the surface, and newly develops a highly safe and efficient deodorant system. Is set as its purpose.

[0004]

Means for Solving the Problems The present inventors have conducted various studies in order to achieve the above object, and found that yeasts, vibrio bacteria, activated sludge and other microorganisms are used as sewage treatment bacteria. Focusing attention, and further, the present inventor, when searching for a strain useful as a sewage treatment bacterium or a deodorant bacterium, can be used as a sewage treatment bacterium to significantly reduce scum, and We succeeded in isolating a strain with remarkable deodorizing effect from soil.

The strain isolated here was recognized as belonging to Bacillus subtilis in terms of mycological properties. However, the superior usefulness such as reduction of scum and deodorization was confirmed by the conventional strain of Bacillus subtilis. Therefore, this isolate was identified as a new strain of Bacillus subtilis, and was named Bacillus subtilis kubota.
This strain has been deposited as Bacillus subtilis Kubota FERM P-9643 at the Institute of Biotechnology (now Institute of Biotechnology, Institute of Technology).

Then, a cloth diaper is immersed in a suspension of Bacillus subtilis kubota (hereinafter, also referred to as the present strain or BSK bacterium), and then dried in the shade,
Using the obtained diaper, we obtained a very useful new finding that no foul odor was observed despite the adherence of excrement.

In addition to the direct deodorizing effect of directly applying BSK bacteria to deodorize the present invention, by applying a wallpaper impregnated with BSK bacteria to a wall, a house, a hospital, a school, a vehicle, etc. As a result of further studies, it was found that the BSK bacterium also has a completely new deodorizing effect, that is, the so-called indirect deodorizing effect of removing the malodor in various rooms.

That is, the present invention relates to a biodeodorant containing Bacillus subtilis kubota and its use. Hereinafter, the present invention will be described in detail.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, as a deodorant microorganism, Bacillus subtilis kubota (BSK bacterium: F
ERM P-9643) is used. As described above, the BSK bacterium is recognized as belonging to Bacillus subtilis in terms of mycological properties, but is characteristic in that it has excellent utility such as deodorization. Its mycological properties are as follows.

(A) Morphology 1 Cell shape and size Polymorphism of short bacilli, 1.2-1.4 × 3.6-3.8 μm 2 cells There is no special case. Involved in the cell membrane and has a nucleus. Also,
It has spores and is characteristic, and Septum can be observed. 3 Existence of mobility There is mobility. The flagella are about ten. 4 Spore presence Spores are present. 5 Gram staining Gram positive. Stained blue with Faber G Set (Nissui). 6 Acid-Resistant Red stained by teal and Nelsen staining. It is an acid-fast bacterium.

(B) Growth state in each medium: Inoculated on a broth agar plate in a state of being suspended in physiological saline,
Incubate at 37 ° C for 24 hours and grow well. This shows an R-type in which the periphery of the settlement does not become circular. The color is opaque, pale yellow. Sheep blood agar culture, heart-in fusion agar culture, and tryptosome agar culture show R type, opaque, and pale yellow, and all have good growth. The growth state in each medium is as follows. Normal agar medium (+) Sheep blood agar medium (+) Heart infusion (+) Tryptic soya (+) Sabouraud (-) Salted egg agar medium (-) Drigalski (-) Brainheart infusion liquid medium (+)

(C) Physiological properties (1) Reduction of nitrate (+) (2) VP test (+) (3) Indole formation (-) (4) Hydrogen sulfide formation (-) (5) Starch Hydrolysis (+) (6) Utilization of citric acid (+) (7) Dye formation Water-soluble (8) Urease (-) (9) Oxidase (+) (10) Catalase (+) (15) Attitude to oxygen Aerobic (16) OF test F

[0013]

(D) Other properties Decomposition of esculin (+) Decomposition of cellulose (+) Utilization of malonic acid (+) Decomposition of arginine (-) Decarboxylation reaction of lysine (+) Decarboxylation reaction of ornithine (-) Deamination reaction of phenylalanine (-) Hemolytic β-type hemolytic auxotrophy (+) β-galactosidase (-)

The new isolate of the present invention, Bacillus subtilis
Kubota grows well in any medium as long as it is a general nutrient medium. In particular, chicken manure extract, animal manure extract, etc. are excellent extracts. Culturing is at 20 to 40 ° C, preferably 30 to
It grows well even if it is left open for about 10 to 24 hours at about 38 ° C.

Since the obtained culture contains a large amount of cells, it can be added and used as it is. Also,
The culture can be washed by separating the cells by centrifugation or the like, and the cells themselves can be used as the culture.
The cells may be in the form of a paste such as a wet cake in this way, or may be freeze-dried, or may be diluted in the opposite direction to a suspension to form the processed product. It is also possible to use. Of course, the same processed product of the culture can be used as well.

BSK bacteria (including cultures and processed products thereof) are contained in water or a liquid containing a paste to prepare a deodorant. For the preparation of deodorant, BSK bacteria are 10 ⁵ to 1
It may be contained in an amount of about 0 ¹⁵ / L, but BSK bacteria can be contained in a large amount because it is effective even in a very small amount and there is no problem in safety. Absent.

As the deodorant, in addition to suspending BSK bacteria in a required amount of water as described above, and further firmly adhering BSK bacteria to fibrous products, etc., a paste is used in an amount of 0.001 to 20.
%, Preferably about 0.01 to 5%. Adhesive content, adhesiveness, influence on BSK bacteria,
It may be determined in consideration of economic efficiency and the like, and in some cases, it may deviate from the above range.

As the paste, a synthetic paste and / or a natural paste is appropriately used. As the synthetic paste, polyvinyl acetate, polyvinyl alcohol, acrylic ester, phenol resin, urea resin, alginic acid, cellulose type (nitrocellulose, cellulose acetate, ethyl cellulose, CMC, water-soluble cellulose, etc.), synthetic rubber type and other viscosities All the synthetic pastes that it has can be used. As the natural paste, in addition to glue, starch, dextrin, rosin, shellac, etc., an inorganic paste such as sodium silicate can be used.

The deodorant thus prepared is usually
It is used by diluting it to a concentration of 1 to 50%, preferably 5 to 20%. When the malodor is strong, the concentration may be higher than the above range, and when the malodor is weak, the concentration is lower than the above range. It may be a concentration.

The deodorant with or without concentration adjustment is applied to the fibrous product to produce a deodorant fibrous product. The application method includes all methods that can bring the deodorant into contact with the fibrous product, such as dipping, spraying, coating, and roller treatment. After application, the product is
Alternatively, the required amount of water is dehydrated or dried to obtain deodorant fibrous products in various water states.

The fibrous products include all fibrous products manufactured from cloth, paper, non-woven fabric, synthetic fibers, blended products, (composite material) laminated products, and the like, for example, fibrous sanitary products and underwear. , Fibrous sheets and the like. Non-limiting specific examples are as follows.

(1) Fibrous sanitary products Diapers, toilet paper, various toilet covers,
Sanitary ware; Tissue paper, Nuretissue, Oshibori; Kitchen paper, paper towel, fukin; Towel, bath towel, sports towel, handkerchief; Foot wipes, sheets, carpet, carpet, futon cover,
Pillowcases, insoles, etc.

(2) Underwear Socks, shirts, pants, other underwear, pajamas, yukata, uniforms, jerseys, judo clothes, kendo clothes,
Other sportswear etc.

(3) Fibrous sheet Wallpaper, ceiling paper, shoji paper, fusuma paper, interior sheet, etc.

The deodorant fiber product produced in this manner is
Even if dirt, filth, excrement or the like adheres, the action of BSK bacteria does not generate a bad odor, and bed sores are suppressed, so to speak, a direct deodorizing effect is achieved. Although the effect continues, if necessary, a deodorant may be filled in a spray can together with a propellant according to a conventional method, and sprayed at a necessary place and a necessary number of times. Also, after washing
It can be reused by applying a deodorant. On the contrary, in the case of products such as toilet paper, tissue paper and other disposable type products, which are finished with a single use, the dose or concentration may be lower than the above.

BS used for the deodorant according to the present invention
K strain K has an almost infinite LD ₅₀ in the oral administration test in mice, and no change was observed in the mouse skin application test, and there is no problem in safety. However, it has no side effects on humans or animals, and can be used safely in underwear. Furthermore, the BSK bacterium has been tested for safety at the Tokyo Food Research Institute, Tokyo Metropolitan Food Sanitation Association, a laboratory designated by the Minister of Health and Welfare, and the safety of this bacterium has been confirmed from this point. .

In the present invention, as described above, the direct contact between the bad odor generating source such as dirt and the BSK bacteria causes
In addition to being able to directly deodorize, if you use this as wallpaper such as deodorant fibrous sheet, you can smell indoor cigarettes, pet odors, musty odors, other room odors, toilet odors, It has the outstanding feature of being able to deodorize the space by deodorizing the odor of the kitchen, the bad odor in the garbage collection room of the building, and so on, so to speak. Also in this case, as in the above case, the biodeodorant may be appropriately applied if necessary, and at that time, it may be used together with the paint. Hereinafter, examples of the present invention will be described.

[0029]

Embodiment 1 The medium having the above composition was placed in a 500 ml flask, and Bacillus subtilis Kubota FERM P-9643 was placed in this.
Was inoculated and statically cultured at 37 ° C. for 20 hours. The obtained culture was centrifuged to separate the cells. On the other hand, using polyvinyl acetate as a water-soluble synthetic paste, a 5% aqueous solution was prepared in advance, and the separated bacterial cells were added to this paste-containing solution and mixed to produce a deodorant.

[0030]

Example 2 The number of BSK bacterial cells obtained in Example 1 was 10 ¹⁰ cells /
A 10% aqueous solution of a deodorant adjusted to L was produced, and cotton socks were immersed in the solution, then spin-dried and dried in the shade to produce deodorant socks. Twenty office workers had these deodorant socks worn all day long. After returning home, the smell of socks was smelled, and as a result, 16 people did not recognize the generation of malodor. Therefore, we again conducted a panel test using deodorant socks.
A spray agent consisting of a 10% deodorant solution was sprayed once. As a result, the remaining four persons did not recognize the generation of malodor.

[0031]

[Example 3] After the sheets were immersed in the deodorant aqueous liquid used in Example 2 (however, no paste was used), centrifugal dehydration was performed.
It was dried in the shade to produce a deodorant sheet. The deodorant sheets were used by 10 bedridden elderly people for 1 week. As a result, although it was soiled, no bad odor was observed from the sheets, and on the contrary, the bad odor was reduced so that it was not noticeable in the entire hospital room.

[0032]

[Example 4] The restaurant was divided into two parts, and in one section, the deodorant aqueous solution used in Example 2 was used for the wallpaper and ceiling sheet (provided that the deodorant aqueous solution was 15% aqueous solution, polyvinyl acetate was used instead of polyvinyl acetate). Alcohol was used) was applied with a roller. The other category served as a control and was not treated with a deodorant. I was asked to do business in this restaurant for 10 days as usual, but during the test period, there was no smell of cigarettes in the room treated with the deodorant, and I felt refreshed. On the other hand, the control area was full of tobacco odor and other complex odors.

[0033]

EFFECTS OF THE INVENTION Various textile products using BSK bacteria have a strong deodorizing property, and not only directly deodorize the bad odor caused by the dirt attached to them but also exist in the space. It also absorbs and deodorizes the bad odors that are produced, so to speak, it can indirectly deodorize, which is very excellent.

Further, the details of the deodorizing mechanism according to the present invention have to be investigated in the future, but it is completely different from the conventional masking with a chemical substance such as a fragrance or the imparting of a strong fragrance, and the odor itself. The present invention can be used widely and safely because it does not cause any problem in safety, and it greatly contributes to the field of pollution prevention technology, medical hygiene technology, nursing care technology, etc. .

Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI Technical display area D06M 16/00 D21H 5/22 C (C12N 1/20 C12R 1: 125)

Claims (6)

[Claims] 1. A deodorant comprising Bacillus subtilis kubota having the following mycological properties in water or a paste-containing liquid. (A) Morphology 1 Cell shape and size Short bacilli, 1.2-1.4 × 3.6-3.8 μm 2 Cell polymorphism Not particularly present. Involved in the cell membrane and has a nucleus. Also,
It has spores and is characteristic, and Septum can be observed. 3 Existence of mobility There is mobility. The flagella are about ten. 4 Spore presence Spores are present. 5 Gram staining Gram positive. Stained blue with Faber G Set (Nissui). 6 Acid-Resistant Red stained by teal and Nelsen staining. It is an acid-fast bacterium. (B) Growth state in each medium Inoculated on a gravy agar plate in a state of being suspended in physiological saline,
Incubate at 37 ° C for 24 hours and grow well. This shows an R-type in which the periphery of the settlement does not become circular. The color is opaque, pale yellow. Sheep blood agar culture, heart-in fusion agar culture, and tryptosome agar culture show R type, opaque, and pale yellow, and all have good growth. The growth state in each medium is as follows. Normal agar medium (+) Sheep blood agar medium (+) Heart infusion (+) Tryptic soya (+) Sabouraud (-) Salted egg agar medium (-) Drigalski (-) Brainheart infusion liquid medium (+) (c ) Physiological properties (1) Reduction of nitrate (+) (2) VP test (+) (3) Formation of indole (-) (4) Formation of hydrogen sulfide (-) (5) Hydrolysis of starch (+) (6) Utilization of citric acid (+) (7) Formation of dye Water-soluble (8) Urease (-) (9) Oxidase (+) (10) Catalase (+) (15) Attitude to oxygen Aerobic (16) OF test F (D) Other properties Decomposition of esculin (+) Decomposition of cellulose (+) Utilization of malonic acid (+) Decomposition of arginine (-) Decarboxylation reaction of lysine (+) Decarboxylation reaction of ornithine (-) Desorption of phenylalanine Amino reaction (-) Hemolytic β-type hemolytic auxotrophy (+) β-galactosidase (-) 2. A Bacillus subtilis kubota isolate, a culture containing the bacterium, and / or sola.
The deodorant according to claim 1, which is a treated product of. 3. A deodorant fibrous product obtained by applying the deodorant according to claim 1 or 2. 4. The deodorant fibrous product according to claim 3, wherein the fibrous product is a deodorant fibrous sanitary product. 5. The deodorant underwear, wherein the fibrous product is deodorant underwear.
Deodorant textile product described in. 6. The deodorant fibrous product according to claim 3, wherein the fibrous product is a deodorant fibrous sheet.