JP2000129294A - Cleaning and deodorizing method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To efficiently clean and deodorize a urinal by continuously or intermittently supplying a liquid containing Bacillus subtilis Kubota (hereinafter referred to as BSK) from the upper side of the urinal. SOLUTION: BSK is internationally entrusted as FERM BP-5847. BSK prevents the formation of a urolith, decomposes a urolith formed, or separates and removes it from the wall of a urinal, thus removing a bad odor, too. BSK grows well on any commonly used nutrient medium, At least one form of BSK selected from among an isolate, a culture medium containing it, and their treating products is used. The use of a solution containing BSK in a number of 2×107-2×1011 per liter is preferable in terms of effectiveness and economy. Preferably, 500 mL of the BSK-containing solution with the above concentration is supplied continuously or intermittently over 30-40 days.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method of purifying and deodorizing urinals, and more particularly, to a method for purifying a toilet bowl.
Subtilis kubota (Bacillus subtil)
The present invention relates to a biopurification / deodorization system characterized by using is Kubota).

[0002]

2. Description of the Related Art A large and small urinal of a toilet gradually becomes yellowish-brown with the elapse of use time, and at the same time, an odor is generated. Such developments are no exception in flush toilets, and they occur frequently not only in household toilets but also in particularly frequently used stations, vehicles, hotels, restaurants, schools, hospitals, etc., and after use. Urinals with a small amount of flowing water are remarkable, and countermeasures are strongly required.

[0003]

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned demands in the art, and has been made for the purpose of newly developing a method for efficiently purifying and deodorizing a toilet bowl.

[0004]

Means for Solving the Problems In order to achieve the above object, the present inventors have decided to use biotechnology instead of chemicals from the viewpoints of safety and prevention of pollution. As a result of examining and broadly selecting and screening the microorganisms of the present invention, the present inventors isolated a new strain Bacillus subtilis kubota (Bacill).
us subtilis Kubota) (deposited internationally as FERM BP-5847 at the Institute of Biotechnology and Industrial Technology, National Institute of Advanced Industrial Science and Technology).

[0005] When a conventional toilet cleaner is used for a culture solution of Bacillus subtilis kubota (hereinafter also referred to as this strain or BSK bacterium), yellow coloring cannot be removed and malodor can be suppressed. When dripping from above the urinal along the wall, the yellowness was found to be unexpectedly reduced.

[0006] Then, when the dropping of the culture solution of BSK bacteria was continued, it was confirmed that the yellowness was further reduced, and finally the whiteness was restored to the original color tone of the toilet bowl, and at the same time, no odor was generated. As one of the mechanism of action by BSK bacteria, it has been confirmed that BSK bacteria prevent urine stone formation or decompose the generated urine stone or peel off and remove it from the toilet bowl wall, and as a result, also remove odor. In addition, BSK bacteria not only endure long-term storage, their activity is not affected by excrement such as urine, and even if water is flushed after each use of urinal, It was also confirmed that the effect was sufficiently maintained if this strain was supplied.

The present invention has been finally completed as a result of further studies based on these useful new findings, that the BSK bacteria are particularly suitable for purifying and deodorizing toilet bowls. Hereinafter, the present invention will be described in detail.

[0008] The present invention is not only characterized in that a particular microorganism is used, but also characterized in its use. First, as microorganisms, Bacillus Su
btilis Kubota (BSK bacteria: FERM B)
P-5847). Although BSK bacteria are recognized as belonging to Bacillus subtilis in general mycological properties, they have an excellent utility of purifying and deodorizing toilet bowls containing urine stone prevention / decomposition / separation effects. It is characteristic. Its mycological properties are as follows:

(A) Morphology 1 Cell shape and size Short bacillus, 1.2-1.4 × 3.6-3.8 μm 2 cell polymorphism There is no particular type. Involved in the cell membrane and has a nucleus. Also,
It has spores and is characteristic, and Septum can be observed. 3 Existence of mobility There is mobility. The flagella are about ten. 4 Spore presence Spores are present. 5 Gram staining Gram positive. Stained blue with Faber G Set (Nissui). 6 Acid-Resistant Red stained by teal and Nelsen staining. It is an acid-fast bacterium.

(B) Growth state in each culture medium Inoculated on a gravy agar plate in a state of being suspended in physiological saline,
Incubate at 37 ° C for 24 hours and grow well. This shows an R-type in which the periphery of the settlement does not become circular. The color is opaque, pale yellow. Sheep blood agar culture, heart-in fusion agar culture, and tryptosome agar culture show R type, opaque, and pale yellow, and all have good growth. The growth state in each medium is as follows. Normal agar medium (+) Sheep blood agar medium (+) Heart infusion (+) Tryptic soya (+) Sabouraud (-) Salted egg agar medium (-) Drigalski (-) Brain heart infusion liquid medium (+)

(C) Physiological properties (1) Nitrate reduction (+) (2) VP test (+) (3) Formation of indole (-) (4) Formation of hydrogen sulfide (-) (5) Starch Hydrolysis (+) (6) Utilization of citric acid (+) (7) Formation of pigment Water-soluble (8) Urease (-) (9) Oxidase (+) (10) Catalase (+) (11) Growth Acidity Poor Neutral Good Temperature 37 ℃ Good 30 ℃ Good 25 ℃ Good (12) Attitude to oxygen Aerobic (13) OF test F

(14) Generation of acid and gas from saccharides Acid gas L-arabinose (−) (−) D xylose (−) (−) D glucose (+) (−) D fructose (+) (−) Maltose (+) ) (−) Sucrose (−) (−) lactose (−) (−) trehalose (±) (−) D sorbit (−) (−) D mannitol (−) (−) Inosit (−) (−) Starch (-) (-)

(D) Other properties Decomposition of esculin (+) Decomposition of cellulose (+) Utilization of malonic acid (+) Decomposition of arginine (-) Decarboxylation of lysine (+) Decarboxylation of ornithine (-) Phenylalanine deamination (-) Hemolytic β-type hemolysis Nutritional requirement (+) β-galactosidase (-)

The new isolate of the present invention, Bacillus subtilis.
Kubota grows well in any nutrient medium. In particular, chicken manure extract, animal manure extract, etc. are excellent extracts. Culture is performed at 20 to 40 ° C, preferably 30 to 40 ° C.
Proliferates well at about 38 ° C. even when left open for 10 to 24 hours.

[0015] The obtained culture contains a large amount of bacterial cells and can be used as it is. In addition, the culture may be separated and washed well by centrifugation or the like, and the cells themselves may be used as culture cells, or the cells may remain in a paste state such as a wet cake. Alternatively, it may be freeze-dried or, conversely, diluted to form a suspension and used in the form of a processed product. Of course, the same treatment of the culture can be used as well.

The BSK bacterium (content, culture, treated product) thus prepared may be applied to a urinal as it is, but usually contains BSK bacterium such as a suspension or culture of BSK bacterium. Use it as a liquid. In that case, 1 BSK bacteria
It is preferable to use a liquid containing 2 × 10 ^{7 to} 2 × 10 ¹¹ (preferably about 100 to 500 million) per liter in terms of both efficiency and economy.

A liquid containing the above-mentioned concentration of BSK bacteria is evenly distributed along the wall from above the urinal as much as possible (not only the part visible from the outside of the toilet bowl wall, but also the inside part (rim) , Including the bottom plate, etc.) in a continuous or intermittent manner. At this time, as a mode of the supply, a BSK bacteria-containing liquid may be dropped or supplied at a time at a certain time interval (intermittent supply).
Alternatively, a fixed amount can be continuously supplied without interruption (continuous supply).

The supply of the BSK bacteria-containing liquid to the urinal can be carried out in a manual manner using a spoiler, a sprayer, or the like, or can be carried out automatically using a drip dispenser. In the latter case, any of commercially available products that supply a fragrance, a coloring agent, a toilet detergent, a disinfectant or the like to a toilet bowl alone or with washing water can be used,
In the present invention, it is only necessary to replace the bottle mounted inside with a bottle of the BSK bacteria-containing liquid, and it is excellent in that existing equipment can be used as it is.

The drip dispenser may be installed anywhere above the urinal. In the case of ordinary equipment, the liquid outlet of the drip dispenser is often connected to the flushing water supply pipe above the face of the urinal, and is open. It is durable, and the effect does not decrease even if flush water is flushed after using the flush toilet. On the contrary, by flushing water, every corner of the toilet bowl has a BS.
K bacteria spread or destroyed by BSK bacteria,
Purification and deodorization of the urinal are performed more efficiently because the exfoliated urine stone is washed away or the formation of urine stone in the trap below the toilet bowl is suppressed.

As described above, even if the BSK bacteria are diluted with the washing water or come into contact with the washing water, the action thereof is not affected at all, and the same applies to the contact with urine or toilet detergent. . On the contrary, the BSK-containing solution contains B
In addition to SK bacteria, water, culture solution, etc., it is also possible to prepare a formulation by adding a coloring agent, a flavoring agent, a surfactant, a soap, a bactericide, a chelating agent and the like.

BSK bacteria have a high viability by nature and retain sufficient activity from February to March even when formulated as described above, and remain sufficiently active after being supplied to the urinal as described above. The activity is maintained. Therefore, in the normal case, as the bottle for the drip dispenser of the BSK bacteria-containing liquid having the above cell concentration, it is possible to adjust the pore diameter of the bottle opening so that the 500 ml bottle is consumed in 30 to 40 days. As a result, it is very convenient in terms of maintenance, such as replacing the bottle about once a month.

The details of the mechanism of purifying and deodorizing urinals by BSK bacteria must be covered in future studies, but it is considered that one of the causes is to prevent, decompose and exfoliate urine stones efficiently. Urinary stones are a cause of urinal discoloration and odor, but at present, BSK bacteria not only prevent urine stones from forming themselves, but also dead carcasses of bacteria act as an adhesive to cause urine While the stones are firmly attached, it is considered that one cause is that urinary stones are peeled off from the vessel wall by the predation or decomposition of the dead bodies of the various bacteria by BSK bacteria.

[0023] Incidentally, BSK bacterium to be used in the present invention, in mice oral study, LD ₅₀ is substantially infinite, also not observed at all change the mouse skin coating test, no problem about the safety Because there is no
Even when the amount of microorganisms used is greater than the above, it does not cause any side effects on humans or animals, and it is safe to attach directly to the skin and can be used with confidence. Furthermore, BS
The K-bacterium has been tested for safety at the Tokyo Food Research Institute, Tokyo Food Sanitation Association, which is an inspection organization designated by the Minister of Health and Welfare, and the safety of this bacterium has been confirmed from this point.

As described above, the present invention has been described with respect to a urinal, but the present invention can be used for a urinal other than a urinal.
It can be used in other toilets besides flush toilets. Next, examples of the present invention will be described.

[0025]

Example 1 Starch 10 g Meat extract 3 g Peptone 10 g Sodium chloride 5 g Water 1000 ml (pH 7.0) A medium having the above composition was put into a 500 ml flask, and Bacillus subtilis kubota FERM BP-584 was added thereto.
7 and inoculated at 37 ° C. for 20 hours. The obtained culture was centrifuged to separate the cells. About 1.5 × 10 ⁹ obtained cells were suspended in 500 ml of natural water, and
A K-containing solution was prepared.

[0026]

Example 2 Approximately 120 million cells obtained in Example 1 were suspended in 470 ml of natural water and a small amount of fragrance for toilet was added to prepare a BSK bacteria-containing liquid, which was then placed in a plastic bottle. , A bottle containing a BSK bacteria-containing liquid (BSK bio-bottle) was manufactured, attached to a commercially available drip dispenser, and a urinal washing / deodorizing test was performed.

When the BSK bio-bottle is inserted into the drip dispenser, a hole is opened in the permeation seal (sealing), and the BSK bacteria-containing liquid flows out and is stored in the receiving tray to maintain a constant water level. When the BSK-containing liquid reaches the bottleneck, a vacuum is created, and further outflow of the BSK-containing liquid is stopped. Next, the BSK bacteria-containing liquid moves to the inside small saucer along the core, and flows out of the outlet provided in the saucer through the tube to the urinal. Usually, the tube is connected and opened to a flushing water supply pipe provided above the urinal, and when water is flowed after use, the water and the water are spread over the entire wall of the urinal, and the washing / deodorizing action is performed. Further promoted, the exfoliated urine stones and the like are washed away and cleaned.

When the BSK-containing liquid flows out and the water level in the outer tray decreases, air enters the bottle, and the BSK-containing liquid automatically comes out of the bottle by the volume. Therefore, the BSK bacteria-containing liquid is supplied to the toilet without special operation. In addition, the above-mentioned wick not only supplies the BSK bacteria-containing liquid to the toilet, but also has an evaporator function, and the fragrance contained in the BSK bacteria-containing liquid evaporates to give a refreshing scent to the toilet. Let it diverge.

In this way, the apparatus was operated for 30 days to completely use up the BSK bacteria-containing liquid in the BSK bio-bottle. After 30 days, the vessel wall did not turn yellow, and no urinary stone formation was observed in the vessel wall, the rim section, the perforated plate section, or the trap section provided at the lower part of the urinal.
No odor was generated for a day. In contrast, B
In the urinal which was not treated with the SK bacteria, urine stone could not be removed even though the urinal was washed with a toilet detergent every three days with a brush.

[0030]

According to the present invention, not only urinary stones can be prevented but also decomposed and separated in urinals and other urinals in both flush toilets and non-flush toilets for the first time. Deodorization can be maintained and achieved over a long period of time.

Further, the method of the present invention can be used as it is without modifying the existing equipment (for example, by simply replacing the chemical bottle mounted on the drip dispenser with the BSK biobottle according to the present invention), and has a long life. There is no need to perform any special maintenance over the period, and the maintenance and management are excellent.

Continued on the front page (51) Int.Cl. ⁷ Identification FI FI Theme Court II (Reference) // (C12N 1/20 C12R 1: 125) (72) Inventor Mikio Sugawara 2-17 Kanayamacho, Higashikurume-shi, Tokyo 8 Clean Life Service Co., Ltd. (72) Inventor Mitsuo Kitazawa 5-24-14 Higashiikebukuro, Toshima-ku, Tokyo F-term (reference) 2D038 AA03 BC01 4B065 AA19X AC12 AC13 AC20 BA22 BC03 BC13 BD15 CA54 4H003 DA06 DB01 DC01 EB39 ED02 FA27 4H011 AA05 BB21 BC18 DA15 DD07 DG03

Claims (5)

[Claims] 1. A method for purifying and deodorizing urinals, comprising continuously or intermittently supplying a liquid containing Bacillus subtilis kubota having the following mycological properties from above the urinal. (A) Morphology 1 Cell shape and size Short bacillus, polymorphism of 1.2-1.4 × 3.6-3.8 μm 2 cells Not particularly. Involved in the cell membrane and has a nucleus. Also,
It has spores and is characteristic, and Septum can be observed. 3 Existence of mobility There is mobility. The flagella are about ten. 4 Spore presence Spores are present. 5 Gram staining Gram positive. Stained blue with Faber G Set (Nissui). 6 Acid-Resistant Red stained by teal and Nelsen staining. It is an acid-fast bacterium. (B) Growth state in each medium Inoculated on a gravy agar plate in a state of being suspended in physiological saline,
Incubate at 37 ° C for 24 hours and grow well. This shows an R-type in which the periphery of the settlement does not become circular. The color is opaque, pale yellow. Sheep blood agar culture, heart-in fusion agar culture, and tryptosome agar culture show R type, opaque, and pale yellow, and all have good growth. The growth state in each medium is as follows. Ordinary agar medium (+) Sheep blood agar medium (+) Heart infusion (+) Tryptic soya (+) Sabouraud (-) Salted egg agar medium (-) Drigalski (-) Brainheart infusion liquid medium (+) (C ) Physiological properties (1) Reduction of nitrate (+) (2) VP test (+) (3) Formation of indole (-) (4) Formation of hydrogen sulfide (-) (5) Hydrolysis of starch (+) (6) Utilization of citric acid (+) (7) Generation of pigment Water-soluble (8) Urease (-) (9) Oxidase (+) (10) Catalase (+) (11) Growth Acidity Poor Neutral Good Temperature 37 (12) Attitude to oxygen Aerobic (13) OF test F (14) Generation of acid and gas from saccharides Acid gas L-arabinose (-) (-) D-xylose (-) −) D glucose (+) (−) D fructose (+) (−) Maltose (+) (−) Sucrose (−) (−) Lactose (−) (−) Trehalose (±) (−) D Sorbit ( -) (-) D-mannitol (-) (-) Inosit (-) (-) Starch (-) (-) (d) Other properties Decomposition of esculin (+) Decomposition of cellulose (+) Use of malonic acid (+) Decomposition of arginine (-) Decarboxylation of lysine (+) Decarboxylation of ornithine (-) Deamination of phenylalanine (-) Hemolytic β-type hemolysis auxotrophy (+) β-galactosidase (-) 2. The method according to claim 1, wherein the Bacillus subtilis kubota is at least one selected from an isolated bacterial body, a culture containing the same, and a processed product thereof. Method. 3. A liquid containing 2 × 10 ⁷ to 2 × 10 ¹¹ Bacillus subtilis kubota per liter as a liquid containing Bacillus subtilis kubota,
The method according to claim 1, wherein: 4. The method according to claim 3, wherein 500 ml of the Bacillus subtilis kubota-containing liquid according to claim 3 is continuously or intermittently supplied over 30 to 40 days. 5. The method according to claim 1, wherein urine is purified and deodorized by preventing and / or removing urine stones.