JP5046666B2 - Antibacterial method and microorganism powder, microorganism-containing liquid agent, microorganism-containing gelling agent - Google Patents

Description

The present invention relates to an antibacterial method capable of suppressing the growth of fungi existing in a substantially sealed space, a microbial powder, a microbial-containing liquid agent, and a microbial-containing gelling agent.

  Conventionally, various types of antibacterial agents, antifungal agents, and deodorants have been developed and used. For example, Patent Document 1 describes an antibacterial and antifungal agent containing triiodoallyltetrazole as an active ingredient. In addition, antibacterial agents using heavy metals such as silver, copper and zinc, and antibacterial methods and deodorizing methods using substances (proteins) produced by microorganisms are also known.

Japanese Examined Patent Publication No. 4-34548

Antibacterial agents, antifungal agents, deodorizers and the like using synthetic compounds and heavy metals as described in Patent Document 1 often have safety problems. In addition, the conventional antibacterial method and deodorizing method are effective when the active ingredient is directly adsorbed on and decomposed by fungi and odorous components. Because it is effective only for the purpose, it is rare that the effect reaches every corner of the target space.
Therefore, the present invention provides an antibacterial method and a deodorizing method that are excellent in safety and effective in every desired space, and provide a microbial powder, a microbial-containing liquid agent, and a microbial-containing gelling agent. Objective.

  As a result of diligent research, the present inventors have found that volatile components generated from microorganisms have an action of suppressing the growth of fungi or decomposing odorous components via the air, leading to the present invention. .

  That is, the antibacterial method of the present invention is characterized by suppressing the growth of fungi existing in a substantially sealed space in a non-contact state by a volatile component generated from a microorganism.

The microorganism was received on January 22, 2007 after depositing with a new Gram-positive spore bacterium belonging to the genus Bacilluc pumilus (National Institute of Advanced Industrial Science and Technology, Patent Biological Deposit Center). can be used a novel microorganism) having entrusted number FERM P -21,162. This microorganism has the characteristic of generating volatile components that suppress the growth of fungi or decompose odorous components.

  Moreover, the deodorizing method of the present invention is characterized by decomposing odorous components present in a substantially sealed space in a non-contact state.

  According to the present invention, volatile components generated from microorganisms are adsorbed via air to fungi and odor components present in a substantially sealed space, and the growth of fungi is suppressed or the odor components are decomposed. Therefore, it is possible to obtain an effect in every corner of the space by simply placing the microorganisms in a substantially sealed space without performing operations such as spraying or applying with a brush.

  The microorganism powder of the present invention is a novel microorganism belonging to the genus Bacillus pumilus that generates volatile components that suppress the growth of fungi existing in a substantially sealed space or decompose odorous components, or two kinds thereof The above microorganisms are dried in a state of being supported on a microorganism carrier.

  Here, the microbial carrier means one having the ability to retain microorganisms, specifically, porous glass, ceramics, metal oxide, activated carbon, kaolinite, bentonite, zeolite, silica gel, alumina, anthracite. , Particulate carriers such as perlite, gel carriers such as starch, agar, chitin, chitosan, polyvinyl alcohol, alginic acid, polyacrylamide, carrageenan, agarose, gelatin, ion-exchanged cellulose, ion-exchange resin, cellulose derivatives, glutaraldehyde Polyacrylic acid, urethane polymer, etc. can be used. Natural or synthetic polymer compounds are also effective, and papers made from cotton, hemp, pulp materials mainly composed of cellulose, or polymer acetates modified from natural products can be used. Furthermore, fabrics made of synthetic polymers such as polyester and polyurethane can also be used. These have good adhesion of microorganisms and preferably have fine gaps. It is more preferable to use a fine material that can easily penetrate during injection.

  According to the present invention, a novel microorganism belonging to the genus Bacillus pumilus that generates a volatile component that suppresses the growth of fungi existing in a substantially sealed space or decomposes an odorous component, or two or more kinds including the same Since microorganisms can be handled as powder, for example, the microorganism powder of the present invention is filled in a cloth such as a woven fabric or a non-woven fabric and placed in a substantially sealed space or filled in a container. By simply placing it, it is possible to obtain an effect in every corner of the space, and handling becomes easy.

  Moreover, it can be set as the microorganisms containing liquid agent which can be used as an antibacterial agent and a deodorizing agent by mixing microorganisms and calcium oxide aqueous solution. Moreover, it can also be set as the microorganisms containing gelling agent which can be used as an antibacterial agent or a deodorizing agent by making a water absorbing polymer absorb this microorganisms containing liquid agent.

  Here, the water-absorbing polymer is not particularly limited, and a known water-absorbing polymer can be used, and any material such as a starch-based polymer material, a cellulose-based polymer material, or a synthetic polymer can be used. May be. In order to increase the adhesion area of microorganisms, it is desirable to use a water-absorbing polymer in the form of granules.

  By mixing a solution containing microorganisms and an aqueous calcium oxide solution, the microorganism-containing liquid agent and the water-absorbing polymer that has absorbed this liquid agent can also decompose acidic odor components such as formaldehyde by the action of calcium oxide. . Therefore, according to the present invention, in addition to the suppression of fungal growth and decomposition of odorous components in a substantially sealed space, the environment in the space is further improved by decomposing formaldehyde, which is a causative substance of sick house syndrome. It becomes possible to improve. In addition, as a calcium oxide aqueous solution, the aqueous solution obtained by baking a shell, etc. can be used, for example.

  According to the present invention, volatile components generated from microorganisms can suppress the growth of fungi existing in a substantially sealed space in a non-contact state, or decompose odorous components. It can be a highly safe antibacterial and deodorizing method without using compounds or heavy metals.

  Hereinafter, embodiments of the present invention will be described in detail.

Example 1
In the embodiment described below, as a microorganism for generating a volatile component degrades the growth inhibition or odor component, fungi, the consignment number FERM P -21 162, a novel microorganism (hereinafter belonging to Bacillus pumilus genus, "the bacterium 1).) Was used.

1-1: Mycological properties Table 1 shows the mycological properties of the present fungus 1 used in this example.

1-2: Isolation method In addition, this microbe 1 is isolated as follows.
First, sewage samples were collected from many grease traps, and the diluted solution was inoculated into a fat and oil decomposition test medium (medium consisting of Tween 20 and inorganic salts) and then cultured at 30 ° C. for 3 days. Then, using a platinum wire from the medium in which a colony of oleolytic bacteria was obtained with a high number of bacteria, using a platinum wire, suspending this in sterile physiological saline, inoculating into a gravy agar medium, forming a colony, About 100 pure bacterial strains were obtained. These bacterial isolates were tested for fat, starch, and protein resolution, and several strains with strong resolution in all three were obtained. Further, a test on pathogenic factors (hemolytic toxin, DNA-degrading enzyme, and emetic toxin gene) was performed, and a strain having no pathogenic factor was selected to obtain bacterium 1.

1-3: Quantitative test method for antibacterial effect Table 2 shows the test bacteria used in this test. As shown in Table 2, the bacteria of numbers 1 to 4 are filamentous fungi (molds), and in particular, the numbers 1 to 3 are environmental resident bacteria that often propagate on the walls of rooms and bathrooms. About these, when a person inhales a spore, allergy (asthma) may develop. Numbers 3 and 4 are plant pathogens, and number 5 is a yeast (baker's yeast). Numbers 6 and 7 are bacteria, number 6 is a non-pathogenic bacterium, and number 7 is a food poisoning bacterium.

  Test bacteria shown in Table 2 were prepared to 50 to 100 cells or spores / 100 μl to prepare a bacterial suspension. This bacterial suspension was smeared in PDA medium for fungi Nos. 1 to 5 and in NA medium for bacteria Nos. 6 and 7 to obtain test container 1. In addition, after culturing the bacterium 1 in NB liquid medium at 30 ° C. with shaking for about 14 hours until the late logarithmic growth phase, 200 μl of this culture solution was inoculated into NA medium and incubated at 30 ° C. for 1-2 days. Test container 2 was obtained. The test container 1 and the test container 2 were placed in a plastic sealed container and cultured at 30 ° C. The number of culture days was 7 days for the fungi Nos. 1 to 5 and 3 days for the bacteria Nos. 6 and 7 (hereinafter referred to as “treatment section”).

  On the other hand, only the test container 1 was placed in a plastic sealed container similar to the above and cultured at 30 ° C. In the same manner as described above, the culture days were 7 days for the fungi Nos. 1 to 5 and 3 days for the bacteria Nos. 6 and 7 (hereinafter referred to as “control group”). For each of the treated group and the control group, the number of colonies of the test bacteria that had grown was counted, and the degree of growth inhibition was calculated by the following equation (1).

Growth inhibition (%)
= (1-average number of colonies in treated area / average number of colonies in control area) x 100 (1)
In addition, about the fungi of No. 1-5, the number of spores was measured with the hematometer, and about the bacteria of No. 6 and 7, the number of bacteria was measured by the direct counting method.

1-4: Results of quantitative test Table 3 shows the results of the quantitative test. FIG. 1 shows a photograph of the test container 1 in the control group and the treatment group in the case of filamentous fungi of Nos. 1, 3 and 4.

  As shown in Table 3, extremely strong antibacterial activity was observed against the four types of filamentous fungi of Nos. 1 to 4 and the yeast of No. 5. Although the detailed mechanism in this regard has not yet been clarified, it is estimated from the test method that the volatile components generated from the bacterium 1 inoculated into the medium placed in the sealed container are transferred to the agar medium through the air. It is considered that the growth of the test bacteria was suppressed due to adsorption to the test bacteria smeared on That is, it can be seen that by using the present fungus 1, the growth of the fungus can be suppressed without direct contact with the target fungus. Therefore, it is possible to suppress the growth of fungi such as filamentous fungi and yeasts existing in the space only by placing the main fungus 1 in a substantially sealed space.

  From the above test results, it can be said that the present fungus 1 is useful for decomposing components that cause odor because the growth of fungi 1 can be suppressed against fungi.

(Example 2)
2-1: Method for producing microbial powder 350 g of pearlite, which is a porous carrier, was impregnated with 1 liter of a microbial culture solution for about 12 hours. Thereafter, the carrier carrying the microorganisms was forcibly dried to reduce the water content to 10% or less to obtain a microorganism powder.

2-2: Quantitative test of antibacterial effect of microbial powder The same test as the quantitative test of the antibacterial effect shown in Example 1 was performed on the microbial powder. In the case of this example, a test container 2 was prepared by spreading 1.5 g of the above microbial powder on an NA medium.

2-3: Results of quantitative test Table 4 shows the results of the quantitative test. As shown in Table 4, extremely strong antibacterial activity was observed against the four types of filamentous fungi of Nos. 1 to 4 and the yeast of No. 5.

(Example 3)
Production method of microorganism-containing liquid agent A microorganism-containing liquid agent can be obtained by mixing 0.1% of a microorganism culture solution with 80% of the calcined calcium solution. An appropriate amount of a plant enzyme may be mixed in this solution. As a result, in addition to the decomposition of odorous components by volatile components by microorganisms, it is possible to decompose acidic odorous components by the action of calcined calcium solution, and to decompose alkaline odorous components by the action of plant enzymes, synergistically It is possible to obtain an advantageous effect.

Example 4
Production method of microorganism-containing gelling agent A suitable amount of the microorganism-containing liquid agent produced in Example 3 was impregnated into a granular water-absorbing polymer.

  According to the present invention, as an antibacterial method and a deodorizing method that are excellent in safety and effective in every corner of a target space, it is particularly preferably used in a substantially sealed space such as a bathroom or a closet. it can.

The photograph of the test container 1 of the control group in the case of the filamentous fungi of Nos. 1, 3, and 4 and the treatment group is shown.

Claims (5)

Antibacterial characterized by suppressing the growth of fungi existing in a substantially sealed space in a non-contact state by a volatile component generated from a novel microorganism belonging to the genus Bacillus pumilus of accession number FERM P-21162 Method. A novel microorganism belonging to the genus Bacillus pumilus , under accession number FERM P-21162, which generates a volatile component that suppresses the growth of fungi existing in a substantially sealed space . A microorganism powder dried in a state where the novel microorganism according to claim 2 and two or more kinds of microorganisms containing the novel microorganism are supported on a microorganism carrier. A microorganism-containing liquid obtained by mixing the microorganism or microbial powder according to claim 2 and an aqueous calcium oxide solution.   A microorganism-containing gelling agent obtained by causing the water-absorbing polymer to absorb the microorganism-containing liquid agent according to claim 4.

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