JPH09263541A - Cosmetic composition and medicinal composition containing aqueous extract of selaginella moellendorfii hieron - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare a cosmetic composition and a medicinal composition having antiallergic and antioxidant actions without any problems in safety. SOLUTION: The cosmetic composition and medicinal composition comprise an aqueous extract of Selaginella moellendorfii Hieron belonging to the genus Selaginella of the family Selaginellaceae or its fractionated substance alone or two or more thereof in combination as an active ingredient in an amount of 0.001-10wt.% in the total amount. The aqueous extract is obtained by extracting a fragment or a pulverized raw or dried root, leaf or stem part of Selaginella moellendorfii Hieron at ambient temperature to 100 deg.C for 1hr to 1 weak with water in an amount of 1-30 pts. based on 1 pt. root, leaf or stem part thereof. The fractionated substance is prepared by further partitioning the aqueous extract with a solvent and separating and fractionating the resultant fraction according to a chromatography. A usually used ingredient can further suitably be blended therewith and prepared as a dentifrice, a mouthwash, a gel, an ointment, a lotion, a cosmetic oil, a cream, a milky lotion, a pack, a powder, a hair tonic, a shampoo, a rinse, a treatment, a mousse, a plaster, a juice, a candy, a chewing gum, a tablet, a troche, etc.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a water extract of the genus Selaginella genus Selaginella genus Selaginella, which is highly safe and has antiallergic and antioxidant effects, or a fraction thereof. And a pharmaceutical composition.

[0002]

2. Description of the Related Art Recently, allergic diseases such as hay fever and atopic dermatitis tend to increase. These allergic reactions are classified into type I to type IV allergic reactions, including type I allergy in which IgE antibody binds to the surface of mast cells via a receptor and causes antigen-antibody reaction due to invasion of antigen. However, in reality, since some of these reactions are combined to cause human lesions, no sufficiently effective antiallergic agent has been found yet.

On the other hand, the skin is an organ in which active oxygen is particularly liable to be generated since it is directly stimulated by external environmental factors such as ultraviolet rays. It is said that the active oxygen generated in the living body is usually eliminated by enzymes such as catalase and superoxide dismutase. However, in vivo,
If such action is insufficient or active oxygen is excessively present, various tissue disorders such as lipid peroxide production, stains and wrinkles are likely to occur.

Antioxidants for preventing and treating these disorders include synthetic products such as dibutylhydroxytoluene and butylhydroxyanisole, and natural product-based products such as tocopherol and rosmarinic acid. Although the effect is strong, its use is limited due to its safety to the living body, and it is said that natural products are safer than synthetic products. There is a problem that it is inferior to synthetic products in terms of stability and effect.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic composition containing an active ingredient of a natural product which exhibits anti-allergic and antioxidant effects and is safe from the viewpoint of living body. And to provide a pharmaceutical composition.

[0006]

In view of the present situation, the present inventors have considered that the histamine release inhibition test and the hyaluronidase inhibitory activity test are indicators of antiallergic action, and the SOD activity test (NTB method) is an indicator of antioxidant action. As a result of a search using the above, it was unexpectedly found that the water extract and the fraction thereof of dog caterpillar have excellent antiallergic and antioxidant effects, and the present invention has been completed.

[0007] That is, the present invention provides a cosmetic composition containing a water extract of the dog caterpillar of the genus Selaginella of the genus Selaginaceae or a fraction thereof. Further, the present invention provides a pharmaceutical composition containing an aqueous extract of dog caterpillar of the genus Selaginella of the genus Selaginaceae or a fraction thereof. Such compositions are useful as antiallergic or antioxidant agents.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION
laginella moellendorfii Hieron (Chinese name: Gangnammaki) is a plant belonging to the genus Seraginella (Selaginella) and is distributed in most of the southern region in China, and is known as the ground bark or ground branch , It is mainly used as a traditional Chinese medicine with hemostatic effect for various bleeding, jaundice, and attracting children.

The water extract of Acacia nigra of the present invention is obtained by extracting raw or dried plants with water. As the plant body, any of a root portion, a leaf portion and a stem portion may be used. The extraction conditions are not particularly limited, but for example, 1 part of dried dogtooth catfish (fine pieces or crushed) to 1 part of water
Add 30 parts and extract in the temperature range of room temperature to 100 ° C for about 1 hour to 1 week with or without stirring,
It can be obtained by filtering or centrifuging the extract to remove solid matter, and then distilling off water by an appropriate method known per se.

The water extract of the dogtooth caterpillar of the present invention can be fractionated by a conventional separation / fractionation technique such as liquid-liquid partitioning using various solvents and chromatography. It is obtained by fractionation.

These water extracts of Aedes aegypti and their fractions may be used alone or in combination of two or more, and the total amount is preferably 0.001 to 10% by weight. If it is less than 0.001% by weight, the antiallergic effect and antioxidant effect cannot be obtained, while if it exceeds 10% by weight, skin irritation becomes high.

The composition of the present invention can be in the form of any oral preparation, external preparation, or internal preparation, which is combined with other desired components by a method known per se, and toothpaste, mouthwash, gel, ointment. Of lotion, cosmetic oil, cream, emulsion, pack, powder, hair tonic, shampoo, rinse, treatment, mousse, patch, juice, candy, chewing gum, tablet, troche, etc. It can be in a pharmaceutical form. In addition, the water extract and the fractionated product of the dog squirrel that are added to the composition of the present invention are derived from natural products, and no toxicity is observed.

The composition of the present invention is applied to skin, scalp, hair, buccal cavity and oral administration.

Hereinafter, the present invention will be described in more detail based on test examples and examples. However, the present invention is not limited to the following examples, and may be appropriately modified within the scope of the invention. It can be implemented.

[0015]

【Example】

(1) Preparation of water extract of dog caterpillar and fractions thereof (Reference Example 1) Water extract 200 g of dried dog caterpillar was appropriately cut and reflux extracted three times with 3 liters of distilled water (2 h). The combined solution of the three extracts was concentrated under reduced pressure and then dried to give 15.0 g of water extract.
I got (Yield 7.49%)

Reference Example 2 Fractionated product 1.0 g of the extract obtained in Reference Example 1 was added to 100 g of distilled water.
ml was added, and liquid-liquid partition was carried out with butanol 100 ml (x3), and each partition liquid was concentrated under reduced pressure to obtain a water fraction of 0.11.
g, butanol fraction 0.65 g was obtained.

(2) Efficacy test example 1 Antiallergic action 1. Histamine release inhibitory effect In order to investigate the anti-allergic effect, a test was conducted to inhibit histamine release by compound 48/80 from rat intraperitoneal mast cells. The type I allergic reaction is caused by the release of chemical mediators such as histamine and leukotriene (degranulation) as a result of the antigen-antibody reaction on the surface of mast cells. Therefore, the measurement was performed by using the inhibitory action of histamine, which is one of the chemical mediators released from mast cells when an inflammatory agent (compound 48/80) was administered, as an index.

Histamine release inhibition test: rat (Wistar
/ ST system, body weight about 250g), and intraperitoneally 0.1% BS
A phosphate buffer was injected, and mast cells were separated according to a conventional method to prepare a cell suspension. The extract obtained in Reference Example 1 was added to this suspension (2 ml), and 10 minutes later, the inflammatory agent (comp
ound 48/80) was added, and 10 minutes later, the amount of free and intracellular histamine was quantified by the fluorescence method. As a control, physiological saline was added instead of the test sample. The release inhibition rate (%) was calculated by the following formula.

[0019]

[Equation 1]

The test results are shown in FIG. From FIG. 1, it can be seen that the water extract of dog caterpillar, which is the active ingredient in the composition of the present invention, suppresses the release of histamine.

2. Hyaluronidase inhibitory activity Hyaluronic acid, which retains the water content of human skin and blood vessels, and thus maintains flexibility, is a macromolecule in which N-acetyl-D-glucosamine and D-glucuronic acid are alternately bonded, and hyaluronidase It is hydrolyzed and converted into low molecular weight compounds. In order to investigate the allergic effect, hyaluronidase (Sigma, Type IV) from beef testis was used to compound
The inhibitory effect of 48/80 on the activation stage of the inactive enzyme was treated with Morgan as the terminal N-acetylglucosamine of the hydrolyzate.
-Measured by quantification by applying the Elson method (Yumie Maeda et al .: Shokuei, Vol. 31, 233-2).
37, 1990).

Hyaluronidase inhibitory activity test: Reference Example 1
An appropriate amount of the extract and the fraction obtained in 2 and 2 were dissolved in 200 μl of 0.1M acetate buffer (pH 4.2) and placed in a test tube, and the enzyme was dissolved in 100 μl of the same buffer to obtain 0.04 m of the enzyme.
After adding g (32 unit) and incubating at 37 ℃ for 20 minutes, compound dissolved in 200 μl of the same buffer solution
Add 48/80 (20 μg) and incubate at 37 ° C. for 20 minutes. Finally, hyaluronic acid sodium salt (200 μg) dissolved in 500 μl of the same buffer was added and incubated at 37 ° C. for 40 minutes.
After adding 200 μl of aOH and water and ice-cooling, 200 μl of borate buffer (pH 9.1) was added and boiled for 3 minutes. After ice cooling, P-dimethylaminobenzaldehyde test solution 3 ml
Was added and incubated at 37 ° C. for 20 minutes,
A ₅₆₅ was measured. As a control, the above acetate buffer was used instead of the sample solution. Also, as each blank,
The above acetate buffer was used instead of the enzyme solution. The inhibitory activity was shown by the inhibition rate calculated from the following formula.

[0023]

[Equation 2] A: Absorbance of control solution B: Absorbance of blank of control solution C: Absorbance of sample solution D: Absorbance of blank of sample solution

Test results: Shown in FIG. In FIG. 2, a is a water extract of dog caterpillar, b is a fraction of a water layer obtained by further liquid-liquid partitioning the water extract of dog caterpillar in water-butanol,
c shows a fraction of the butanol layer obtained by further liquid-liquid partitioning the water extract of dog caterpillar into water-butanol. Also,
The horizontal axis represents the final concentration of the test sample. From FIG. 2, it can be seen that the dog extract of Acacia nigra, which is an active ingredient in the composition of the present invention, exhibits hyaluronidase inhibitory activity.

(3) Efficacy Test Example 2 Antioxidant Action In order to examine the antioxidant action, an SOD activity measurement test by the NBT method was carried out. The measurement was carried out by adding azo blue nitroblue tetrazolium (NBT) to a test sample solution in which superoxide radicals were generated by xanthine-xanthine oxidase, and measuring the absorbance.
The principle is to measure the trapped amount of superoxide radicals (SOD activity) by the change in absorbance.

SOD activity test: 0.05M Na ₂ CO ₃
Buffer (pH 10.2) 2.4 ml, 3 mM xanthine solution 0.1 ml, 3 mM EDTA aqueous solution 0.1 ml, 0.1
5 wt% bovine serum albumin aqueous solution 0.1 ml, 0.75 m
The final concentration of 0.1 ml of the M NBT aqueous solution and 0.1 ml of the extract solution obtained in Reference Example 1 was 0.025 mg / ml, 0.1.
05mg / ml and 0.1mg / ml were mixed,
After leaving it at 25 ° C for 10 minutes, the absorbance in the blank test was 0.
0.1 ml of xanthine oxidase (manufactured by Sigma) prepared so as to have a concentration of 20 to 0.23 was added, and the mixture was incubated at 25 ° C for 20 minutes. After that, 6 mM Cu
The enzyme reaction was stopped by adding 0.1 ml of Cl ₂ solution to 560 n.
The absorbance at m was measured (A). Further, as a control, the same operation was performed using a diluting solvent instead of the sample solution (B). As a blank for each sample, after adding a test sample solution, 0.1 ml of a 6 mM CuCl ₂ solution was added, and similarly, 0.1 ml of xanthine oxidase was added to measure the absorbance (C). The inhibition rate (%) was calculated by the following formula.

[0027]

(Equation 3) A: Absorbance of sample by enzyme reaction B: Absorbance of control by enzyme reaction C: Absorbance of sample by non-enzyme reaction

Test results: Shown in FIG. In FIG. 3, the horizontal axis represents the concentration of the test sample. From FIG. 3, it can be seen that the water extract of dog caterpillar, which is an active ingredient in the composition of the present invention, exhibits an SOD activity inhibitory action, and thus an antioxidant action.

(4) Examples (Example 1) Toothpaste components Blending amount (wt%) Water extract obtained in Reference Example 1.0 Calcium carbonate 45.0 Sodium carboxymethyl cellulose 1.0 Glycerin 20.0 Lauryl Sodium sulphate 1.5 Sodium saccharin 0.1 Perfume 1.0 Purified water balance Total 100.0 A toothpaste was prepared by a conventional method according to the above formulation.

(Example 2) Toothpaste component Blending amount (wt%) Water extract obtained in Reference Example 1.0 Dibasic calcium phosphate 45.0 Sodium carboxymethyl cellulose 0.5 Glycerin 20.0 Sodium lauryl sulfate 1. 5 Saccharin sodium 0.1 Fragrance 0.5 Purified water Remainder total 100.0 According to the above formulation, a toothpaste was prepared by a conventional method.

(Example 3) Powdered dentifrice components Blending amount (wt%) Water extract obtained in Reference Example 2.5 Dicalcium phosphate 55.0 Calcium carbonate 30.0 Sorbitol 10.0 N-lauroyl sarcosine sodium 0 .5 Sucrose fatty acid ester 1.0 Saccharin sodium 0.1 Fragrance 0.5 Purified water balance Total 100.0 According to the above-mentioned formulation, a tooth powder was prepared by a conventional method.

(Example 4) Mouthwash component Blending amount (wt%) Water extract obtained in Reference Example 1.5 Ethanol 10.0 Saccharin sodium 0.2 Polyoxyethylene hydrogenated castor oil 2.0 Glycerin 5.0 Fragrance 1.0 Purified water balance Total 100.0 According to the above formulation, a mouthwash was prepared by a conventional method.

Example 5 Gel Oral Agent Component Ingredient Amount (wt%) Water Extract Obtained in Reference Example 5.0 Saccharin Sodium 1.0 Perfume 0.1 Polyethylene Glycol 400 50.5 Polyethylene Glycol 4000 43 .4 Total 100.0 According to the above formulation, a gel oral preparation was prepared by a conventional method.

Example 6 Ointment Ingredients Amount (wt%) Water Extract Obtained in Reference Example 1.0 Propylene Glycol # 400 15.0 Macrogol Ointment 84.0 Total 10.0 An ointment was prepared by the method.

(Example 7) Lotion component Blending amount (wt%) Water extract obtained in Reference Example 0.5 Glycerin 6.0 Ethanol 8.0 Polyexethylene Ethylene hydrogenated castor oil 0.8 Methylparaben 0.05 Quen Acid 0.05 Sodium citrate 0.07 Perfume 0.1 Purified water balance Total 100.0 According to the above formulation, a lotion was prepared by a conventional method.

(Example 8) Ingredient for cosmetic oil Component content (% by weight) Fraction of water extract obtained in Reference Example 2.0 Cholesteryl stearate 1.0 Olive oil 2.0 Squalane Residual total 100. 0 A cosmetic oil was prepared by a conventional method according to the above formulation.

Example 9 Cream Ingredients Amount (wt%) Ingredient (A) Fraction of water extract obtained in Reference Example 2.0 Salami beeswax 4.0 Cetanol 2.0 Stearic acid 1.0 Isopropyl myristate 5.0 Lanolin 2.0 Liquid paraffin 9.0 Self-emulsifying glyceryl monostearate 3.0 Polyoxyethylene sorbitan monostearate 1.5 Component (B) Methyl paraoxybenzoate 0.1 Propylene glycol 5.0 0 Fragrance 0.2 Purified water balance Total 100.0 According to the above formulation, a cream was prepared by a conventional method.

(Example 10) Emulsion component Blending amount (wt%) Component (A) Fraction of water extract obtained in Reference Example 0.2 Stearyl glycyrrhetinate 0.1 Liquid paraffin 5.0 Vaseline 2. 0 beeswax 1.0 sorbitan sesquioleate 2.0 component (B) polyethylethylene oleyl ether 2.5 ethyl paraoxybenzoate 0.1 propylene glycol 5.0 carboxyvinyl polymer 0.5 potassium hydroxide 0.5 perfume 0 .2 Purified water balance Total 100.0 According to the above-mentioned prescription, an emulsion was prepared by a conventional method.

(Example 11) Pack component Blending amount (% by weight) Fraction of water extract obtained in Reference Example 5.0 Vinyl acetate / styrene copolymer 10.0 Polyvinyl alcohol 10.0 Solbit 5.0 0 allantoin 0.5 kaolin 7.0 ethanol 5.0 fragrance 2.0 ethyl paraoxybenzoate 0.1 purified water balance 100.0 According to the above-mentioned prescription, a pack was prepared by a conventional method.

(Example 12) Powder component Blending amount (wt%) Fraction of water extract obtained in Reference Example 1.0 Dextrin 96.0 Talc 2.0 Decaglyceryl stearate 1.0 Total 100. 0 According to the above formulation, a pouter was prepared by a conventional method.

Example 13 Chewing Gum Ingredients Amount (wt%) Water Extract Obtained in Reference Example 10.0 Gum Base 65.0 Mannitol 20.0 Sorbit 3.5 Perfume 1.5 Total 100.0 Above A chewing gum was prepared by a conventional method according to the recipe.

Example 14 Gargling Tablets Ingredients Amount (wt%) Water Extract Obtained in Reference Example 5.0 Sodium Bicarbonate 55.0 Sodium Diphosphate 15.0 Polyethylene Glycol 6000 3.0 Citric acid 12.0 Ethanol 6.0 Fragrance 4.0 Total 10.0 According to the above-mentioned prescription, a mouthwash tablet was prepared by a conventional method.

(Example 15) Ingredient amount of troche (% by weight) Water extract obtained in Reference Example 0.001 Gum arabic 6.0 Fructose 20.0 Glucose 20.0 Maltose 30.0 Perfume 0.1 Purification Water balance Total 100.0 According to the above formulation, a troche was prepared by a conventional method.

Example 16 Hair Tonic Ingredients Amount (wt%) Water Extract Obtained in Reference Example 0.1 Ethanol 70.0 Tocopherol Acetate 0.05 Glycyrrhizic Acid 0.1 Propylene Glycol 3.0 l- Menthol 0.1 Fragrance / Dye Appropriate Fragrance Solubilizer Appropriate Purified Water Remainder Total 100.0 According to the above formulation, a hair tonic was prepared by a conventional method.

[0045]

EFFECTS OF THE INVENTION The cosmetic composition and the pharmaceutical composition containing the water extract of Acacia nigra of the present invention or a fraction thereof have excellent anti-allergic effect and antioxidant effect, and also have a problem in safety. Instead, it can be used as an oral preparation, an external preparation and an internal preparation.

[Brief description of drawings]

FIG. 1 is a graph showing the histamine release inhibitory effect (anti-allergic effect) of an aqueous extract of dog caterpillar.

FIG. 2 is a graph showing the hyaluronidase inhibitory effect (anti-allergic effect) of various components extracted from canine caterpillar.

FIG. 3 is a graph showing the superoxide radical activity inhibitory effect (antioxidant effect) of a water extract of dog caterpillar.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display area A61K 7/26 A61K 7/26 7/48 7/48 9/20 9/20 U

Claims (4)

[Claims] 1. A cosmetic composition comprising an aqueous extract of dog caterpillar of the genus Seraginella of the genus Selaginaceae or a fraction thereof. 2. A pharmaceutical composition containing an aqueous extract of dog caterpillar of the genus Seraginella of the genus Selaginaceae or a fraction thereof. 3. The use according to claim 1, which is used as an antiallergic agent. 4. The method according to claim 1, which is used as an antioxidant.
Uses described.