JPH09255548A - Wrinkling suppressing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a wrinkling suppressing agent by using Thujopsis dolabrata or its extract as an active component. SOLUTION: This agent contains 0.0001-20wt.% (in terms of solid extract) of Thujopsis dolabrata (a plant of the family Cupressaceae) or its extract as an active component. The active component is properly compounded with anti- inflammatory agent, antioxidant, oil, humectant, ultraviolet absorber, alcohol, chelating agent, pH-modifier, antiseptic, thickener, colorant, perfume, etc., and prepared in the form of O/W-type or W/O-type emulsified cosmetic, cream, milky lotion, face lotion, oily cosmetic, pack, foundation, etc. The wrinkling suppressing agent has excellent action to suppress the formation of wrinkles caused by the irradiation with ultraviolet rays and is useful as an external preparation for preventing the aging of the skin, especially preventing wrinkle. Thujopsis dolabrata is used as it is in the form of dried and pulverized leaf, twig, etc., or as an extract obtained extracting crushed leaf, twig, etc., with a solvent (e.g. ethanol).

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a wrinkle formation inhibitor, and more specifically, a lotion, cream, emulsion, pack,
The present invention relates to a wrinkle formation inhibitor that is preferably used for cosmetics such as scalp cosmetics and pharmaceuticals.

[0002]

2. Description of the Related Art The skin is subtly affected by temperature and humidity, ultraviolet rays, aging, diseases, stress, eating habits, etc., which causes various troubles such as deterioration of various functions of the skin and aging of the skin. Therefore, especially in recent years, maintaining healthy and beautiful skin has become a serious concern regardless of age or sex.

Of these, wrinkles, which are one of the problems of the dermis, are aging of the skin due to aging and sunlight (photoaging).
Caused by Although the mechanism of skin aging is not clear, since the skin is located in the outermost layer of the living body and plays a role of the front line of biological defense, accumulation of damage due to environmental factors has a large effect on the skin aging phenomenon. It is believed that
In particular, ultraviolet rays are considered to be the largest environmental factor involved in skin aging and wrinkle formation, and various free radicals (especially superoxide, hydroxy radicals, active oxygen such as singlet oxygen) produced by ultraviolet rays are It is known that not only causes acute inflammation but also induces photoaging by repeating its production chronically. Active oxygen is associated with DNA-protein cross-linking (cross-linking) of the dermal component, damage or denaturation of protein cross-linking of collagen and elastin, inactivation of antioxidant enzymes such as SOD, and membrane lipid peroxidation of cell components and its consequences. It is thought that it causes the deterioration of cell function of erythrocyte, resulting in aging of skin and formation of wrinkles (Fragrance Journal, Vol. 11, 49-54, 1992).

Therefore, in the past, in order to prevent or treat skin aging and the formation of wrinkles, the use of an antioxidant such as vitamin E (JP-A 61-215309, JP-A 62-2631).
No. 10, etc.), utilization of various plant extracts (JP-A-62-61924)
JP, JP-A-63-174911, JP-A-6-65043, etc.), use of extracellular matrix control agent such as collagen (JP-A-4-74016, JP-A-3-20206, etc.) The use of retinoic acid and α-hydroxy acid has been proposed.

[0005]

However, these conventional anti-aging agents or anti-wrinkle agents are not sufficiently effective. Then, the objective of this invention is providing the wrinkle formation inhibitor which was excellent in the wrinkle formation inhibitory effect.

[0006]

In view of such circumstances,
As a result of intensive studies, the present inventors have found that asunaro (Thujopsis dolabrata), a cypress plant, or an extract thereof has an excellent effect of suppressing wrinkle formation, and completed the present invention.

That is, the present invention provides a wrinkle formation inhibitor containing asunaro or an extract thereof as an active ingredient.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION Asunaro is a special genus of Japan and is an evergreen tree that is distributed in Honshu, Shikoku and Kyushu, and its wood is used as Wajima wood. However, as for the biological activity of asunaro, it has an antimicrobial effect (see Japanese Patent Application Laid-Open No. 2-6280).
9), a whitening effect (JP-A-5-345710) and a bone disease preventive effect (JP-A-6-340542), but little has been known so far, and inhibition of wrinkle formation has been reported. Nothing is known about its effect.

In the present invention, asunaro mainly consists of leaves,
The dry pulverized product such as the twig can be used as it is, but it is preferable to use the extract thereof. Asunaro extract should be extracted with the solvent at room temperature or under heating, or with an extractor such as a Soxhlet extractor, after crushing mainly the leaves, twigs, etc. of asunaro without drying or drying. Can be obtained by In addition, asunaro extract in the present invention means various solvent extracts, their diluted solutions, concentrated solutions or dried powders.

As the solvent extract, asunaro or its dried powder is used as water, an organic solvent (petroleum ether, n-hexane, cyclohexane, carbon tetrachloride, toluene, benzene, dichloromethane, chloroform, ether, ethyl acetate, butanol, acetone, propanol). , Ethanol, methanol, pyridine, polyethylene glycol, propylene glycol, butylene glycol, etc.), aqueous alcohol, etc., and usually obtained by an extraction treatment at 3 to 70 ° C.

[0011] As a preferred specific example of extraction from asunaro drug substance, 100 g of dried and crushed asunaro is added to ethanol 1
Examples include a method of immersing in liter, performing extraction for 7 days at room temperature with occasional stirring, filtering the obtained extract, allowing the filtrate to stand at 5 ° C. for 3 days, and then filtering again to obtain a supernatant. .

The above-mentioned extract can be used as it is as an active ingredient of the wrinkle formation inhibitor of the present invention. After the extract is concentrated, it is further separated by a suitable separation means such as gel filtration, silica gel column chromatography or high performance liquid. Fractions with high activity can also be fractionated and used by chromatography or the like.

The above-mentioned asunaro or its extract can be used as it is, or after diluting, concentrating or freeze-drying, preparing it in the form of powder or paste and appropriately formulating it for use. Further, if necessary, it may be used after being subjected to a purification treatment such as deodorization and decolorization using activated carbon or the like.

The blending amount of asunaro or its extract with respect to the wrinkle formation inhibitor of the present invention is not particularly limited, but usually,
The solid content of the extract (dry matter) is preferably 0.0001 to 20% by weight.

The anti-wrinkle agent of the present invention includes not only asunaro or its extract but also allantoin, a vitamin E derivative,
Anti-inflammatory agents such as glycyrrhizin and ascorbic acid derivatives,
By adding antioxidants such as α-tocopherol and ascorbic acid, it is possible to improve the effect of suppressing wrinkle formation, and other oil components and moisturizers (hyaluronic acid, ceramide) commonly used as cosmetic ingredients. etc),
UV absorbers, alcohols, chelating agents, pH adjusters,
Preservatives, thickeners, pigments, fragrances and the like can be combined in any combination.

The wrinkle formation inhibitor of the present invention has various forms and uses, for example, oil / water type, water / oil type emulsified cosmetics, creams, cosmetic emulsions, lotions, oily cosmetics, packs, foundations, etc. Can be used as Further, the wrinkle formation inhibitor of the present invention is not limited to general skin cosmetics, and includes pharmaceuticals, quasi drugs, cosmeceuticals and the like.

[0017]

Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

Production Example 1 Production of Asunaro Extract (1):
1 kg of crushed asunaro (a mixture of leaves and twigs) was immersed in 8 liters of ethanol for 6 hours at room temperature to extract ethanol-soluble components. Next, the same operation was performed again on the residue from which the extract was separated, to obtain a total of 16 liters of the extract. The solvent of this extract was distilled off and dried under reduced pressure to give 80 g of extract.
I got

Production Example 2 Production of Asunaro Extract (2):
1 kg of crushed asunaro (a mixture of leaves and twigs) was immersed in 8 liters of ethanol for 6 hours at room temperature to extract ethanol-soluble components. Next, the same operation was performed again on the residue from which the extract was separated, to obtain a total of 16 liters of the extract. After distilling off the solvent of this extract and concentrating it to 1 liter, 100 g of activated carbon was added, the mixture was stirred for 6 hours, deodorized and decolorized, and then the activated carbon was filtered off, and the filtrate was concentrated under reduced pressure.
73 g of extract was obtained as a syrup.

Test Example 1 Wrinkle formation inhibition test by hairless mice: Hairless mice (HR / ICR, 6 weeks old at the start of the experiment) were placed on the backs of 6 animals per group, and ethanol solution (1% w / v) of asunaro extract or control As ethanol, 8
0 μl was applied. After 2 hours, wipe off the plant extract on the skin surface with an ethanol solution and use a health ray lamp (Toshiba, SE
20) was used to irradiate UV-B light. This operation was performed 5 times a week for 10 weeks. For irradiation, the amount of energy is UV-Radiometer (TOKYO OPTICAL, UVR-305 / 365
The total irradiation amount was 100 mJ / cm ² with an energy amount of 0.28 mW / cm ² so that the irradiation amount per time was 1 MED or less. After the test, the degree of wrinkles formed was divided into the following four stages, and a standard photograph was set for each stage, comparing the degree of wrinkles of each mouse with the standard photograph,
It was evaluated as a wrinkle index. Table 1 shows the results.

<Wrinkle index> 1: Wrinkles are formed slightly 2: Wrinkles are formed at a low degree 3: Wrinkles are formed at a moderate degree 4: Wrinkles are formed at a high degree

Test Example 2 Analysis of wrinkles: In order to analyze the wrinkles formed in Test 1 in detail, each mouse was treated with a Hydrophilic Exaflex hydrophilic vinyl silicone image-forming agent to form a circle with a diameter of 3 cm. A skin replica was collected from a place. This replica was irradiated with light from a 30 ° direction in a horizontal state, and the ratio of shadows formed by wrinkles was obtained as an area ratio using an image analyzer.

[0023]

[Table 1]

Various cosmetics having the following compositions were produced by a conventional method.

Example 1 Cream (composition) (% by weight) Asunaro extract obtained in Production Example 1 Squalane 5 Stearic acid 2 Glycerin monostearate 10 Ethanol 2 Methyl paraoxybenzoate 0.2 Cetanol 2 Olive oil 4 Vaseline 5 Glycyrrhizinate 1 Vitamin E 0.5 Fragrance, trace amount of pigment, trace amount of purified water, balance 100 total

Example 2 Skin lotion (composition) (% by weight) Asunaro extract obtained in Production Example 2 Glycerin monostearate 1 Ethanol 15 Propylene glycol 4 Isopropyl palmitate 3 Lanolin 1 Methyl paraoxybenzoate 0.1 Ceramide 1 Perfume Trace amount Dye Micro- purified water Residual total 100

Example 3 Packing agent (composition) (% by weight) Asunaro extract obtained in Production Example 1 Polyvinyl alcohol 20 Glycerin 5 Ethanol 16 Perfume Trace dye Pigment purified water Residual 100

Example 4 Lotion (Composition) (wt%) Asunaro extract obtained in Production Example 2 1.00 10-Hydroxyundecanoic acid 0.75 9-Hydroxyundecanoic acid 0.20 8-Hydroxyundecanoic acid 0.05 Arginine 0.40 Lysine 0.40 Polyoxyethylene (40) Hydrogenated castor oil 1.50 Methyl polysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3775C, Toray Dow Corning) 0.60 Glycerin 5.00 1,3-Butylene glycol 3.00 Glycine betaine 0.20 Trisodium citrate 0.90 Quent Acid 0.40 Urea 0.50 ε-Aminocaproic acid 0.10 Ethanol 5.00 Preservative Suitable amount Perfume Suitable amount Purified water Remainder 100.00

Example 5 Lotion (composition) (% by weight) Asunaro extract obtained in Production Example 2 0.50 10-hydroxyundecanoic acid 0.89 9-hydroxyundecanoic acid 0.20 8-hydroxyundecanoic acid 0.02 triethanolamine 0.40 potassium hydroxide 0.20 Polyoxyethylene (20) isocetyl ether 0.30 Polyoxyethylene (8) oleyl ether Sodium phosphate 0.30 Polyoxyethylene (10) sodium dialkyl phosphate 0.10 Polyethylene glycol 1500 2.00 Polyoxyethylene (10) methyl glucoside 1.50 Dipropylene glycol 0.50 disodium hydrogen phosphate 0.50 succinic acid 0.30 ethanol 10.00 royal jelly extract 2.00 preservative appropriate amount perfume appropriate amount purified water balance 100.00 total

Example 6 Gel (Composition) (wt%) Asunaro Extract Obtained in Production Example 0.5 10-Hydroxyundecanoic Acid 1.7 9-Hydroxyundecanoic Acid 0.2 8-Hydroxyundecanoic Acid 0.1 Polyoxyethylene (20) Isocetyl Ether 1.0 Sodium polyoxyethylene (10) trialkylphosphate 1.0 Sorbitol 0.5 1,3-Propanediol 0.5 Xanthan gum 0.5 Tuberose polysaccharide 3.0 Carboxyvinyl polymer (Carbopol 940, Goodrich) 0.2 Dipotassium glycyrrhizinate 0.1 Water Potassium oxide 0.9 Allantoin 0.1 Tannic acid 0.2 Ethanol 20.0 Preservative Suitable amount Perfume Suitable amount Purified water Balance 100.0

Example 7 Gel (Composition) (wt%) Asunaro Extract Obtained in Production Example 2 0.20 10-Hydroxyundecanoic Acid 1.50 9-Hydroxyundecanoic Acid 0.40 8-Hydroxyundecanoic Acid 0.10 Silicone Composition (KSG-17, Shin-Etsu Chemical Co., Ltd. 5.00 Methyl polysiloxane (KF96A-6cs, Shin-Etsu Chemical Co., Ltd.) 15.00 Methyl polysiloxane (SH244, Toray Dow Corning Co., Ltd.) 5.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane copolymer (SH3771C) , Toray Dow Corning) 2.00 Methyl polysiloxane methyl (polyoxyethylene) siloxane copolymer (SH3775C, Toray Dow Corning) 1.00 Methyl cellulose 0.20 Hydroxyethyl cellulose Hydroxypropyl trimethylammonium chloride ether (Cathello H-60, Kao) 0.02 acetic acid dl-α-tocophero Le 0.05 glycyrrhetinic isostearyl 0.10 isopropylmethylphenol 0.10 EDTA 0.10 Ethanol 5.00 Preservative qs Perfume qs Purified water Balance Total 100.00

Example 8 Emulsion (composition) (wt%) Asunaro extract obtained in Production Example 2 0.30 10-hydroxyundecanoic acid 1.07 9-hydroxyundecanoic acid 0.36 8-hydroxyundecanoic acid 0.07 N- (3-hexadecyloxy 2-Hydroxypropyl) -N-2-hydroxyethyl decanamide 0.50 N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethylhexadecanamide 1.00 N- (3-tetrade Siloxy-2-hydroxypropyl) -N-2-hydroxyethyl decanamide 0.50 Polyoxyethylene (10) hydrogenated castor oil 1.00 Methyl polysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3775C, Toray Dow Corning Incorporated) 1.00 sorbitan monostearate 0.20 stearoylmethyl taurine sodium 0.50 cholesterol 0.80 cholesterol isostearate 0.20 alkenylsuccinic acid Acid monocholesteryl 0.80 Stearic acid 0.20 Palmitic acid 0.30 Myristic acid 0.10 Pentyl glycol dicapric acid 4.00 Methylpolysiloxane (KF96A-500cs, Shin-Etsu Chemical Co., Ltd.) 2.00 Isostearyl alcohol 1.20 Cetyl alcohol 1.00 Glycerin 3.50 Lactic acid 0.20 Sodium lactate 0.30 Preservatives Fragrance Appropriate amount of purified water balance 100.00

Example 9 Lotion (Composition) (wt%) Asunaro Extract Obtained in Production Example 0.50 7-Hydroxyoctanoic Acid 0.75 7-Hydroxynonanoic Acid 0.20 7-Hydroxydecanoic Acid 0.05 Arginine 0.40 Lysine 0.40 Polyoxyethylene (40) Hydrogenated castor oil 1.50 Methyl polysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3775C, Toray Dow Corning) 0.60 Maltitol 5.00 Ethylene glycol monoethyl ether 0.20 Trisodium citrate 0.90 Succinic acid 0.40 Urea 0.50 ε-Aminocaproic acid 0.10 Ethanol 5.00 Preservative Suitable amount Perfume Suitable amount Purified water Balance 100.00

Example 10 Lotion (Composition) (wt%) Asunaro extract obtained in Production Example 2 0.30 11-Hydroxydodecanoic acid 0.73 11-Hydroxytridecanoic acid 0.32 11-Hydroxytetradecanoic acid 0.03 Diisopropanolamine 0.40 Hydroxylation Potassium 0.20 Polyoxyethylene (20) isocetyl ether 0.30 Polyoxyethylene (8) oleyl ether Sodium phosphate 0.30 Sodium polyoxyethylene (10) dialkyl phosphate 0.10 Polyethylene glycol 1500 2.00 Polyoxyethylene (20) methyl glucoside 1.50 Isoprene glycol 0.50 Disodium hydrogen phosphate 0.50 Succinic acid 0.30 Ethanol 10.00 Yeast extract 2.00 Preservative proper amount Perfume proper amount Purified water Balance 100.00

Example 11 Gel (Composition) (wt%) Asunaro Extract Obtained in Production Example 1.0 Ethyl 10-hydroxyundecanoate 1.7 Glyceryl 9-hydroxyundecanoate 0.2 Hydroxyethyl 8-hydroxyundecanoate 0.1 Polyoxyethylene ( 20) Isocetyl ether 1.0 Polyoxyethylene (10) sodium trialkylphosphate 1.0 Sorbitol 0.5 1,3-Propanediol 0.5 Xanthan gum 0.5 Tuberose polysaccharide 3.0 Carboxyvinyl polymer (Carbopol 940, Goodrich) 0.2 Water Potassium oxide 0.9 Allantoin 0.1 Zinc sulfocarbolate 0.2 Ethanol 20.0 Preservative proper amount Perfume proper amount Purified water Residual total 100.0

Example 12 Gel (Composition) (wt%) Asunaro Extract Obtained in Production Example 2 0.60 Isopropyl 9-hydroxydecanoate 1.50 Glycine 8-hydroxydecanoate 0.40 Ethyl 9-hydroxydodecanoate 0.10 Silicone composition (KSG -16, manufactured by Shin-Etsu Chemical Co., Ltd. 5.00 Methyl polysiloxane (KF96A-6cs, manufactured by Shin-Etsu Chemical Co., Ltd.) 15.00 Methyl polysiloxane (SH244, manufactured by Toray Dow Corning Co., Ltd.) 5.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane co-weight Combined (SH3771C, Toray Dow Corning) 2.00 Methylpolysiloxane-methyl (polyoxyethylene) Siloxane Copolymer (SH3775C, Toray Dow Corning) 1.00 Methylcellulose 0.20 Hydroxyethylcellulose Hydroxypropyl trimethylammonium chloride ether (Cathello H) -60, made by Kao) 0.02 Acetic acid d l-α-Tocopherol 0.05 Isostearyl glycyrrhetinate 0.10 Isopropylmethylphenol 0.10 Zinc white 1.50 Ethanol 5.00 Preservative proper amount Perfume proper amount Purified water Residual total 100.00

Example 13 Emulsion (composition) (wt%) Asunaro extract obtained in Production Example 2 0.50 Isostearyl 10-hydroxyundecanoate 1.25 Ethyl 9-hydroxyundecanoate 0.46 Glyceryl 8-hydroxyundecanoate 0.09 N- (3 -Hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethyldecanamid 0.50 N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethylhexadecanamide 1.00 N- ( 3-Tetradecyloxy-2-hydroxypropyl) -N-2-hydroxyethyl decanamide 0.50 Polyoxyethylene (10) hydrogenated castor oil 1.00 Methyl polysiloxane methyl (polyoxyethylene) siloxane copolymer (SH3773C, Toray)・ Made by Dow Corning) 1.00 Sorbitan monostearate 0.20 Cholesterol 0.80 Cholesterol isostearate 0.20 Alkenyl succinate monocholate Teryl 0.80 Stearic acid 0.50 Squalane 4.00 Methylpolysiloxane (KF96A-500cs, Shin-Etsu Chemical Co., Ltd.) 2.00 2-Ethylhexyl paramethoxycinnamate (Persol MCX-SA, Divodan Co., Ltd.) 2.00 Cetyl alcohol 1.00 Glycerin 3.50 Lactic acid 0.20 Sodium lactate 0.30 Preservative Suitable amount Perfume Suitable amount Purified water Balance 100.00

Example 14 Pack (composition) (% by weight) Asunaro extract obtained in Production Example 2 1.00 10-Hydroxyundecanoic acid 4.00 9-Hydroxyundecanoic acid 0.85 8-Hydroxyundecanoic acid 0.15 Polyvinyl alcohol (Gothenol EG-30, Nippon Synthetic Chemical Industry Co., Ltd.) 15.00 Carboxymethyl chitin aqueous solution (chitin liquid HV-10, manufactured by Ichimaru Falcos) 5.00 Triglucopolysaccharide (Pullulan PI-20, manufactured by Hayashibara) 0.50 Xanthan gum 0.50 Carboxymethylcellulose sodium 0.50 Titanium oxide 15.00 Aluminum magnesium silicate 1.00 1-Isostearoyl-3-myristoyl glycerol 1.00 Diglycerin 1.50 Polyoxyethylene (20) isocetyl ether 1.00 Ethanol 5.00 Preservative proper amount Perfume proper amount Purified water Residual total 100.00

Example 15 Pack (composition) (wt%) Asunaro extract obtained in Production Example 2 1.00 10-Hydroxyundecanoic acid 4.00 10-Hydroxydodecanoic acid 0.85 7-Hydroxydecanoic acid 0.15 Polyvinyl alcohol (Gothenol EG-30, Nippon Synthetic Chemical Industry Co., Ltd.) 15.00 Carboxymethyl chitin aqueous solution (chitin liquid HV-10, manufactured by Ichimaru Falcos) 5.00 Triglucopolysaccharide (Pullulan PI-20, manufactured by Hayashibara) 0.50 Xanthan gum 0.50 Carboxymethylcellulose sodium 0.50 Titanium oxide 15.00 Aluminum magnesium silicate 1.00 1-Isostearoyl-3-myristoyl glycerol 1.00 Diglycerin 1.50 Polyoxyethylene (20) isocetyl ether 1.00 Ethanol 5.00 Preservative proper amount Perfume proper amount Purified water Residual total 100.00

[0040]

INDUSTRIAL APPLICABILITY The wrinkle formation inhibitor of the present invention is excellent in the action of suppressing wrinkle formation due to irradiation of ultraviolet rays, and is useful as an external preparation for preventing skin aging, especially for preventing wrinkles.

 ──────────────────────────────────────────────────の Continuing on the front page (72) Inventor Yoshinori Nishizawa 2606 Kabane-cho, Akaga-cho, Haga-gun, Tochigi Pref.

Claims (1)

[Claims] 1. A wrinkle formation inhibitor containing asunaro or an extract thereof as an active ingredient.