JPH09252647A - Fused mushroom of matsutake fungus and shiitake fungus, its primordium culture method and culture method therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To economically obtain a fused mushroom by mass-producing the fused mushroom of matsutake fungus (Tricholoma matsutake) and shiitake fungus. (dentinus edodes). SOLUTION: This fused mushroom of matsutake fungus and shiitake fungus is obtained by mix-inoculating the matsutake fungus and shiitake fungus to a necessary culture medium in a necessary ratio. The culture method for this fused mushroom is mixing white clay and humus soil to the sawdust of broad leaf trees, making this medium include vitamin, mineral, sugar, etc., and mixing a small amount of slaked lime therewith to sterilize and process it to make the culture medium. Powery matsutake fungus and shiitake fungus are mix- inoculated to this culture medium in the prescribed ratio and a room temperature is kept at 15 deg.C to 25 deg.C. After the culture medium is covered with white hyphae, projecting and recessing parts are generated on the surface of the culture medium, and the surface of the culture medium is colored to be dark brown by further culturing, the room temperature is raised by less than about 5 deg.C compared with the room temperature but to be lower than 28 deg.C to further grow the mushrooms for the necessary number of days. As the result of this culture, mushroom primordium is formed on the surface of the culture medium. The culture medium is washed by water and then moved to a hypha culture place and the room temperature is adjusted to 12 deg.C to 20 deg.C. As a result, mushrooms are grown by breaking pellicles from the surface of the culture medium after several days.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fused mushroom of Matsutake fungi and Shiitake fungi, a method for cultivating the primitive mushroom, and a cultivation method thereof.

[0002]

2. Description of the Related Art Conventionally, it has been impossible to say that a fused mushroom of matsutake fungus, which is a mycorrhizal fungus that parasitizes pine roots, and shiitake fungus, which has a deadly parasitic character.

[0003]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention The present inventor prays to obtain a mushroom that is a fusion of Matsutake fungi and Shiitake fungi, and to obtain a mushroom that can be easily cultured and can be artificially cultivated in a mass-produced manner. As a result of the experiment, we succeeded in obtaining this fused mushroom.

[0004]

[Means for Solving the Problems] That is, in the required medium,
Matsutake fungus and Shiitake fungus were mixed and inoculated at a required ratio to obtain a fused mushroom of Matsutake fungi and Shiitake fungus. This fused mushroom has a lighter scent than real matsutake mushrooms, but its shape is similar to that of real matsutake mushrooms, and the stem is thick and long with a good chewy texture and good taste.

In this fused mushroom of Matsutake fungi and Shiitake fungi, the mixing ratio of the aforementioned Matsutake fungi and Shiitake fungi is 2: 2 by weight.
It is preferably 8 to 5: 5. For example, Matsutake mushroom 2
It is assumed that Shiitake fungi are mixed with 5 to 5 in a ratio of 8 to 5. It should be noted that these matsutake fungi and shiitake fungi may be in powder form or liquid form.

The method of culturing the primordia of the fused mushroom of Matsutake fungi and Shiitake fungus of the present invention is applied to the sawdust of broad-leaved trees such as oak (Kunugi), oak (nara), Yamageki (beech), and shii (Example). Then, for each 2 kg), a mixture of white clay and mulch sold in the home gardening industry (total of 400 g in equal amount each) was added, and vitamins, minerals, sugars, etc. were added as nutrients, and a small amount of slaked lime was mixed. What was mixed and put in a plastic bag and sterilized was used as a medium, and powdered Matsutake fungi and Shiitake fungi were added to the culture medium in the required ratio (weight ratio 2: 8 to 5:
Inoculated with the mixture in 5), kept at a room temperature of 15 ° C to 25 ° C (preferably 18 ° C to 20 ° C), and after about 2 months, the medium is covered with white hyphae to form irregularities on the medium surface, and further cultured. As a result, after about 3 months, the medium surface turns blackish brown, and the room temperature is higher than the room temperature by about 5 ° C. or lower but lower than 28 ° C. (preferably 20 ° C.).
It is said that mushroom primordia are formed on the surface of the medium by further culturing for a required number of days (at about 25 ° C to 25 ° C) (about 2 weeks or more).

[0007] As described above, the method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungus of the present invention comprises mixing sawdust of hardwood with white clay and mulch, and adding vitamins, minerals, sugars, etc. Slaked lime was mixed and sterilized to form a medium, and the medium was inoculated with powdered Matsutake fungi and Shiitake fungi in a required ratio and kept at an indoor temperature of 15 ° C to 25 ° C to give a white mycelium. It is covered with and irregularities are formed on the surface of the medium,
After further culturing, the surface of the medium becomes dark brown. After that, the room temperature is higher than the room temperature by about 5 ° C. or lower but lower than 28 ° C., and further cultivated for the required number of days. After the mushroom primordium is formed on the medium, the medium is taken out, washed with water, transferred to a mycelial culture room, and the room temperature is adjusted to 12 ° C to 20 ° C (preferably 15 ° C to 18 ° C). It is said that mushrooms are generated by breaking the film.

After the fused mushroom is generated as described above and grown to a required size in about 4 to 5 days, the fungus bed can be reused. That is, the bacterial bed after collecting the fused mushrooms was rested for about 2 weeks, soaked in water for several days, and drained, and then the room temperature was kept at 12 ° C to 2 ° C.
By adjusting the temperature to 0 ° C (preferably 15 ° C to 18 ° C) and culturing, mushrooms are generated again from the medium surface after several days (4 to 5 days). In this way, mushrooms can be obtained up to 3 times.

[0009]

According to the present invention, as described above, a fused mushroom of matsutake fungi and shiitake fungi is obtained for the first time. The scent is lighter than that of real matsutake mushrooms, but its shape is similar to that of real matsutake mushrooms, with thick and long stems, sufficient fiber, and a good chewy texture and good taste. As for this fused matsutake mushroom, a small one can be obtained in an amount of 20 g to 30 g and a large one can be obtained in an amount of 50 g to 80 g. The yield is 300g from one bacterial bed
The harvest of 700g is expected and it is a new variety of mushrooms. Also, if you install air conditioning equipment in the mushroom cultivation room,
It can be cultivated throughout the year and can be harvested daily.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The fused mushroom of matsutake fungus and shiitake bacterium according to the present invention is as described above, and examples of the culturing method and cultivating method thereof will be described below. Approximately 2 kg of sawdust (size 2 mm to 3 mm) of broad-leaved trees such as oak, oak, beech, and shii, white clay (commercially available as Tomiyama Horticulture) and pine forest 400 g of each of the mulberry leaves of the same amount were mixed, and 200 g of rice bran and 20 to 30 sugar corn were added to the mixture as nutrients.
g, bran 100 g, calcium chloride [CaCl ₂ .2H ₂ O] as a food additive (manufacturer Katayama Chemical Co., Ltd.) 5 g, oil cake 100 g, and Ebios (manufactured by Asahi Breweries Ltd.) 5 to 8 g as vitamin B are mixed. do it,
Further, 5 to 10 g of slaked lime [Ca (OH) ₂ ] is added, water is added, the mixture is sufficiently stirred with a mixer, placed in a plastic container such as a plastic bag, sterilized under normal pressure, and naturally cooled to room temperature as a medium. To do.

Next, the mouth of the plastic bag is opened, and powdered matsutake fungi and shiitake fungi are mixed and inoculated into the upper part of the medium at a weight ratio of 2: 8. As the matsutake fungi and the shiitake fungi, folds (folds) on the back of the umbrellas of the matsutake mushrooms and shiitake mushrooms were taken, and mycelia were grown and collected on an agar medium containing liquid glucose as a nutrient.

Immediately after completion of the inoculation with the mushroom fungus, the mouth of the plastic bag is closed and the room temperature is maintained at 18 ° C to 20 ° C for culturing. After about 2 months, the medium was covered with white hyphae, and pit-like uneven ridges were formed on the surface of the medium. After further culturing, the surface of the medium became dark brown after about 3 months. After this,
When culturing was performed at a room temperature of 20 ° C to 25 ° C, a mushroom primordium was formed on the surface of the medium in 3 weeks.

After the mushroom primordium is thus formed on the surface of the culture medium, the plastic bag is broken, the medium is taken out and washed with water to prepare for mushroom generation. That is, by moving to a mycelium culture room (bacterial bed culture shelf) and adjusting the room temperature to 15 to 18 ° C., one week later, the film was broken from the surface of the medium and mushrooms were generated.

After the fused mushrooms were generated as described above and grown to a required size in about 4 to 5 days, the fungal beds were reusable. That is, the bacterial bed after collecting the fused mushrooms was rested for about 2 weeks, soaked in water for about 2 to 3 days, and drained, and the room temperature was set at 15 ° C to 1 ° C.
By adjusting the temperature to 8 ° C. and culturing, mushrooms were generated again from the medium surface after 4 to 5 days. In this way, mushrooms were obtained up to 3 times. In the above-mentioned example, the fusion ratio of Matsutake fungi and Shiitake fungi was 2: 8, but the experiment in which the fusion ratio was 5: 5 was also conducted, and the same as the above except that the yield was reduced. Results were obtained.

[0015]

Industrial Applicability According to the present invention, as described above, a fused mushroom of Matsutake fungi and Shiitake fungi is obtained for the first time. The scent is lighter than that of real matsutake mushrooms, but its shape is similar to that of real matsutake mushrooms, with thick and long stems, sufficient fiber, and a good chewy texture and good taste. This fused matsutake mushroom is 20g to 30g for a small one and 50g to 80 for a large one.
Grams can also be obtained. According to the method of the present invention, 300 g to 700 g can be harvested from a single fungus bed, and if an air conditioning facility is provided in the mushroom cultivation room, year-round cultivation can be performed and daily mushroom harvesting is possible. It has a great feature that it is suitable for mass production.

Claims (11)

[Claims] 1. A fused mushroom of matsutake fungi and shiitake fungi obtained by inoculating a required medium with matsutake fungi and shiitake fungi in a required ratio. 2. The fused mushroom of matsutake fungus and shiitake bacterium according to claim 1, wherein the fusion ratio of matsutake bacterium and shiitake bacterium is 2: 8 to 5: 5 by weight ratio. . 3. Mixing white clay and mulch with hardwood sawdust,
In addition to containing vitamins, minerals, sugars, etc., this was mixed with a small amount of slaked lime and sterilized to form a medium, and the medium was inoculated with powdered Matsutake fungi and Shiitake fungi in the required proportions After keeping the temperature at 15 ° C to 25 ° C, the medium is covered with white hyphae to form irregularities on the medium surface, and after further culturing, the medium surface becomes dark brown, and then the room temperature is about 5 ° C lower than the room temperature. Fusion of matsutake fungi and shiitake fungi, characterized in that mushroom primordia are formed on the surface of the medium by culturing for a required number of days at a temperature higher than ℃ or lower but lower than 28 ℃ Mushroom primordium culture method. 4. A mixture of white clay and mulch into hardwood sawdust,
In addition to containing vitamins, minerals, sugars, etc., this was mixed with a small amount of slaked lime and sterilized to form a medium, and the medium was inoculated with powdered Matsutake fungi and Shiitake fungi in the required proportions After keeping the temperature at 15 ° C to 25 ° C, the medium is covered with white hyphae to form irregularities on the medium surface, and after further culturing, the medium surface becomes dark brown, and then the room temperature is about 5 ° C lower than the room temperature. After the mushroom primordium is formed on the surface of the medium by further culturing for a required number of days, the temperature is higher than ℃ or lower but lower than 28 ℃. 12 ℃ ~ 2
A method of cultivating a fused mushroom of Matsutake fungi and Shiitake fungi, characterized in that the mushroom is generated by breaking the film from the surface of the medium after several days by adjusting the temperature to 0 ° C. 5. The method for cultivating a fused mushroom according to claim 4, resting the fungal bed after collecting mushrooms for about 2 weeks, soaking it in water for several days, and draining it to obtain the room temperature. A method of cultivating a fused mushroom of Matsutake fungi and Shiitake fungi, characterized in that mushrooms are generated again from the medium surface after several days by controlling the temperature to 12 ° C to 20 ° C and culturing. 6. The method is characterized in that the room temperature is maintained at 18 ° C. to 20 ° C. when the medium is inoculated with powdered Matsutake fungi and Shiitake fungi in a required ratio and inoculated. The method for cultivating a primordium of a fused mushroom of pine mushroom and shiitake bacterium according to claim 3. 7. The room temperature is 20 ° C. to 25 ° C. when the medium is covered with white hyphae and the surface of the medium is uneven, and the culture is continued until the surface of the medium becomes dark brown. 4. The method for cultivating a primordium of a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 3, characterized in that. 8. The method is characterized in that the room temperature is maintained at 18 ° C. to 20 ° C. when the medium is inoculated with powdered Matsutake fungi and Shiitake fungi in a required ratio and inoculated. The method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 4. 9. The room temperature for culturing until the surface of the medium is dark brown after the medium is covered with white hyphae and the surface of the medium has irregularities is set to 20 ° C. to 25 ° C. The method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 4, characterized in that. 10. The room temperature when the medium is taken out, washed with water and then transferred to a mycelium culturing site for culturing, after the mushroom primordium is formed on the surface of the medium is set to 15 ° C. to 18 ° C. The method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 4, wherein. 11. The room temperature when mushrooms are regenerated after collecting the mushrooms by the method for cultivating the fused mushrooms according to claim 4, wherein the room temperature is 15 ° C. to 18 ° C. The method for cultivating a fused mushroom of matsutake fungi and shiitake fungi according to claim 5.