JPH09117264A - Propolis composition and its production - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a propolis composition in a good yield, having an excellent dispersing property to water and a good stability, and to provide a method for producing the propolis composition. SOLUTION: This propolis composition contains the propolis extract and a saponin. This saponin is a quillaja saponin, a soybean saponin or a carrot saponin. The composition is allowed to contain a sugar, a sugar alcohol or a branched cyclodextrin. Also, the propolis composition is produced by extracting active components in a raw material of the propolis after adding the saponin and an extracting solvent to the raw material of the propolis. Further, the propolis composition containing a low content of ethanol or not containing ethanol is obtained by concentrating the composition containing the propolis extract and ethanol after adding the saponin, the saponin and the sugar, the sugar alcohol or the branched cyclodextrin.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a propolis composition having excellent dispersibility in water and useful as a health food or the like and a method for producing the same.

[0002]

2. Description of the Related Art Propolis is a resinous substance collected from a honeycomb and contains flavonoids, organic acids such as P-coumaric acid and cinnamic acid and esters thereof, various minerals, vitamins and the like. It is known that it has an antibacterial action, an antioxidant action, an anticancer action and an antiaging effect. Although the active ingredients of these propolis are hardly soluble in water, many of them are soluble in organic solvents, so products containing propolis,
That is, ethanol extract of propolis is used for health foods, pharmaceuticals, and cosmetics.

[0003]

However, as described above, most of the components of propolis, including flavonoids, are almost insoluble in water, so that various inconveniences occur when processing or using propolis products. I have. For example,
In producing a drink containing propolis, when a propolis extract is added to an aqueous solution containing other drink ingredients, some of the insoluble components precipitate out as oil. This oily substance not only contaminates equipment and containers and hinders the manufacturing operation, but also reduces the product yield because the precipitated oily component contains a large amount of active ingredients, and reduces the product quality. Come down.

An ethanol extract of propolis is sold as it is as a health food. In this case, the ethanol extract is diluted with water or fruit juice and taken, but not only does the insoluble active ingredient contained in the propolis precipitate in oil, which significantly impairs the image of the product, and also contaminates the container, Or adhere to teeth. Since this deposit cannot be easily removed by washing, it is a problem in using the product.

[0005] The prior art relating to a propolis composition having dispersibility in water and a method for producing the same is disclosed in Japanese Patent Application Laid-open No. Hei 2-
There is a technique described in Japanese Patent No. 245159 (name: propolis food composition and a method for producing the same, hereinafter referred to as a prior patent). Claim 1 of this prior patent discloses "a propolis food composition comprising a monohydric alcohol extract of propolis, a polyol such as glycerin, and a polyol / fatty acid surfactant". Claim 2 of the preceding patent discloses a method for producing the propolis food composition of claim 1.

[0006] The only monohydric alcohol that can be used for food is ethanol, and polyols such as glycerin include sugars and sugar alcohols. The polyol / fatty acid surfactant is a food additive of a chemically synthesized product, and is not suitable for the propolis market for health foods, pharmaceuticals, and cosmetics.

On the other hand, in the conventional product, sucrose,
A production method for removing ethanol from a propolis composition containing a sugar such as glucose or a sugar alcohol such as sorbitol or reduced starch syrup has not been established. For example, in the composition using a polyol / fatty acid-based surfactant as the surfactant component according to the method of Example 1 of the prior patent specification, the propolis component in the concentrate, the saccharide and the sugar alcohol are separated at the stage of removing ethanol. Therefore, a uniform propolis composition cannot be obtained.

In the prior art, no technique has been established for producing a water-dispersible propolis composition in which components contained in a propolis raw material are emulsified or colloided as they are without using a chemically synthesized product. For example, claim 3 of the prior patent
The food composition of the present invention contains multiple hydrogen bonding
Since the dissolving power of the H-based medium is lower than that of ethanol,
A significant portion of the propolis active ingredient is missing.

The present invention has been made in view of the above conventional problems, and a first object thereof is to provide a propolis composition having excellent dispersibility in water and a method for producing the same. Therefore, it is intended to solve the disadvantages in the production of propolis products or the use of propolis products due to the precipitation of oil-insoluble components in water when the propolis composition is diluted with water.

A second object of the present invention is to use a material preferable as a material for health foods, pharmaceuticals and cosmetics, and to contain the active ingredient of propolis as a raw material as it is without degrading, which is of high quality and stable. And to provide a method for producing a propolis composition with high yield.

[0011]

In order to achieve the above object, the propolis composition of the first invention is characterized by containing a propolis extract and saponin. The second invention is characterized in that the saponin of the first invention is quillaja saponin, soybean saponin or ginseng saponin.
A third invention is characterized in that in the first or second invention, it contains a sugar, a sugar alcohol or a branched cyclodextrin.

The method for producing a propolis composition of the fourth invention is characterized in that the active ingredient in the propolis drug substance is extracted after adding an extraction solvent and saponin to the propolis drug substance. In the method for producing a propolis composition having a low ethanol content or no ethanol of the fifth invention, a saponin or a saponin and a sugar or a sugar alcohol or a branched cyclodextrin are added to a composition containing a propolis extract and ethanol. It is characterized by being concentrated.

The propolis extract in the present invention includes both a solution containing an extraction solvent, and a solid or powder from which the solvent has been removed. In the case of a solution, the concentration of propolis is preferably 70% by weight or less in view of viscosity, and in the case of propolis tincture (alcohol solution of propolis) used as a health food, it is usually preferably 10 to 20% by weight.

The solvent used in the present invention may be any solvent, but when the solvent remains, a solvent compatible with water is preferable. Suitable examples are alcohols such as ethanol and methanol, and glycols such as propylene glycol. Ethanol is most suitable for food grade propolis compositions and their manufacture.

The saponin used in the present invention has a function of lowering the interfacial tension, and any one having such activity can be used. Generally, saponin refers to glycosides distributed in plants, and is composed of a hydrophobic aglycone and a hydrophilic sugar moiety. This saponin is not simply a material as a surfactant, but is often a functional food material having an effect as a health food, as typified by ginseng saponin and soybean saponin. Ginseng saponin, soybean saponin, quillaja saponin and the like are suitable for the propolis composition for food and the production thereof. If the required amount as a surfactant is satisfied, the purity of saponin is not particularly specified. For example, with respect to Quillaja saponin, a product containing 25 wt% or 5 wt% of a commercial Quillaja extract can be used as it is.

In the present invention, the amount of saponin used is
Depending on the type and concentration of saponin, propolis extract, sugar, sugar alcohol, and branched cyclodextrin, 0.01% by weight or more of saponin solid content is usually used with respect to the propolis composition.
-10% by weight is used.

As a method of adding saponin, a method of directly adding to the propolis extract or a method of adding a predetermined amount of saponin to an extraction solvent such as ethanol in the extraction step of propolis is employed, whereby the active ingredient from propolis is added. Extraction can be done.

Sugars, sugar alcohols used in the present invention,
The branched cyclodextrin enhances the stability of the aqueous dispersion, and there are no particular restrictions on its purity or type,
Commercially available sugar materials can be used as they are. As the sugar, any of sucrose, glucose, fructose, isomerized sugar, starch syrup, palatinose and the like can be used, and as the sugar alcohol, sorbitol, maltitol, reduced starch syrup, reduced lactose, palatinit and the like can be used. In particular, starch syrup and branched cyclodextrin are suitable for the stability of the emulsification type propolis composition, and in particular, a low saccharified starch syrup having a numerical value (DE value) showing the degree of hydrolysis from starch of 10 to 30 Low saccharified reduced starch syrup equivalent to is suitable.

The amounts of sugar, sugar alcohol and branched cyclodextrin used in the present invention can be used in any concentration depending on the composition of the product. Next, a method for producing the propolis composition will be described.

In the production method of the fourth aspect of the present invention, the active ingredient in the propolis drug substance is extracted after adding an extraction solvent such as ethanol and saponin to the propolis drug substance. In this method, the saponin can be dissolved in the extraction solvent in advance, so that the extraction rate of the active ingredient from the propolis drug substance can be improved.

In the production method of the fifth invention, saponin or saponin and sugar or sugar alcohol or branched cyclodextrin are added to a composition containing a propolis extract and ethanol, and the mixture is concentrated under reduced pressure, for example, by a rotary evaporator. . As a result, the resulting propolis composition has a low ethanol content or no ethanol.

At this time, the saponin's surface-active action, sugar,
The stabilizing action of sugar alcohols and branched cyclodextrins, or their interaction, is expressed. That is, emulsification is carried out without deteriorating the active ingredient of the raw material propolis, and there is no risk of separation even during concentration, so that no tar is generated and therefore concentration is carried out smoothly.

In addition, in order to lower the ethanol content in the propolis composition, it is effective to add water during the concentration. This is because the water forms an azeotropic mixture with ethanol, and the ethanol can be reliably distilled off.

[0024]

In the propolis composition according to the first aspect of the invention, the saponin contained in the propolis composition exhibits an excellent surface-active effect, so that it exhibits excellent dispersibility in water. Second
In the invention described above, since the saponin is quillaja saponin, soybean saponin, or ginseng saponin, this action can be effectively exerted and an effect as a health food can be obtained. Further, in the third invention, the propolis composition is sugar.
By containing the sugar alcohol or the branched cyclodextrin, the stability of the aqueous dispersion obtained by the surface-active action of saponin is improved.

In the method for producing a propolis composition of the fourth invention, a predetermined propolis composition is obtained by adding an extraction solvent and saponin to the propolis drug substance and then extracting the active ingredient in the propolis drug substance. To be In this method, the extraction efficiency of the active ingredient is improved because the extraction is performed by adding both the extraction solvent and the saponin having a surface active action.

In the method for producing a propolis composition of the fifth invention, saponin or saponin and sugar or sugar alcohol or branched cyclodextrin are added to a composition containing a propolis extract and ethanol, and the mixture is depressurized by, for example, a rotary evaporator. A propolis composition can be obtained by concentrating at. At this time, emulsification is carried out without changing the active ingredient of the raw material propolis by the surfactant activity of saponin, the stabilizing effect of sugar, sugar alcohol and branched cyclodextrin, or their interaction, and separation occurs even during concentration. There is no fear and the concentration is done smoothly. Then, a propolis composition having a low ethanol content or no ethanol is obtained.

[0027]

【Example】

(Example 1) 1 kg of Brazilian propolis raw mass was pulverized,
To this was added 2 kg of 95% ethanol, and the mixture was allowed to stand at room temperature for one week with occasional stirring. 2 Separated by filtration through filter paper to obtain a propolis extract. 200 g of Kirayanin C-100 (manufactured by Maruzen Kasei Co., Ltd., containing 25% of Kiraya extract) was added to this propolis extract.
After stirring, the mixture was filtered again, and ethanol was added to adjust the concentration to 20% as a solid content derived from propolis to obtain 2 kg of a tincture-type propolis composition.

The appearance of this propolis composition is the same as ordinary propolis ethanol tincture, and is a brown transparent liquid, which is easily dispersed in water. (Example 2) 9 kg of Chinese raw propolis ingot was crushed and
36 liters of ethanol was added, and the mixture was left at room temperature for one week with occasional stirring. The supernatant was no. 2 Add 2 kg of Kirayanin C-100 to the filtrate separated by filter paper.
Was. After stirring, the mixture was filtered again, and then concentrated under reduced pressure using a rotary evaporator, and the concentration was adjusted to 50% as a solid derived from propolis to obtain 8.1 kg of a concentrate.

20 g of this propolis composition was added to 1 kg of reduced starch syrup S30 (manufactured by Niken Kagaku Co., Ltd.) with stirring. The mixture was stirred at 24,000 rpm for 10 minutes at room temperature using an emulsifier (Ultra Disperser, manufactured by Yamato Scientific Co., Ltd.) to obtain 1.0 kg of an emulsion-type propolis composition.

The emulsion of the propolis composition was stable even when stored for a long period of time. (Example 3) 1 kg of propolis raw mass from Brazil was pulverized,
After adding 2 kg of ethanol and 100 g of Kirayanin C-100, the mixture was allowed to stand at room temperature for one week with occasional stirring. 2 Separated by filtration with filter paper. Then, the concentration was adjusted to 20% as a propolis-derived solid content to obtain 2.2 kg of a tincture-type propolis composition.

During the manufacturing process of this propolis composition
Then, since Quillayanin C-100 was added to ethanol in advance, the extraction efficiency, that is, the yield was improved by 10% (0.2 kg) as compared with Example 1.

To 100 g of the propolis composition, 1 kg of fructose-glucose liquid sugar (concentration: 75%) was added with stirring. 24000 at room temperature with the Ultra Disperser
The mixture was stirred at rpm for 10 minutes to obtain 1.05 kg of an emulsion type propolis composition.

To 1 kg of this propolis composition was added 100 g of soybean saponin (soybean extract manufactured by Koshiro Pharmaceutical Co., Ltd.), and then dispersed in 500 kg of water. To this, 1.5 kg of citric acid, 100 kg of honey and 500 g of ginseng extract (containing ginseng saponin) were added and stirred. Thereafter, the mixture was filtered through a 200-mesh strainer, and then dispensed into glass bottles in 50 ml portions while sterilizing by heating at 90 ° C. for 10 minutes to obtain 10,000 drinks of the propolis composition.

In the manufacturing process, no oily component was precipitated and the emulsion was well dispersed, so that dispensing was easy and a uniform and excellent product was obtained. (Example 4) A propolis extract was prepared in the same manner as in Example 1. The solid content derived from propolis was 24.5%. 100% of this propolis extract contains kirayanin C
100 g of -100 and 5 g of water were added and stirred. To this was added 0.950 kg of branched cyclodextrin (IsoElite 40L, Niken Chemical Co., Ltd.). It concentrated under reduced pressure with a rotary evaporator and the concentration was adjusted to 30% as a solid content to obtain 2.45 kg of a concentrate.

The concentrate thus obtained was a stable emulsion, and when analyzed, no ethanol content was detected. (Example 5) To 100 g of a propolis extract having a solid content of 24.5% derived from propolis prepared in Example 4, 10 g of kirayanin C-100 and 1 kg of water were added and stirred. Reduced starch syrup 30 (manufactured by Niken Chemical Co., Ltd.) 1.0
7 kg was added. Concentrate under reduced pressure with a rotary evaporator to adjust the concentration to 70% as a solid content, and concentrate 1.1.
kg gained.

The concentrate thus obtained was a stable emulsion, and when analyzed, no ethanol content was detected. (Comparative Example 1) Using raw Brazilian propolis as a raw material,
2.5 g of Ryoto Sugar Ester S-1670 (sucrose fatty acid ester of Mitsubishi Kasei Co., Ltd.) was added to 100 g of the propolis extract having a solid content of 20% derived from propolis prepared by the method of Example 1. , Sucrose fatty acid ester was hardly dissolved. When this was stirred and the sucrose fatty acid ester was suspended and poured into water, a large amount of oil component was precipitated. (Comparative Example 2) 2.5 g of Ryuto Sugar Ester S-570 (sucrose fatty acid ester manufactured by Mitsubishi Kasei Co., Ltd.) was added to 100 g of a propolis extract having a solid content of 24.5% derived from propolis prepared in Example 4. And 1 kg of water were added and stirred. 1.07 kg of reduced starch syrup 30 was added to this and concentrated under reduced pressure with a rotary evaporator. During the concentration, the propolis component and the reduced starch syrup component were separated, and when the temperature dropped, the separated component solidified and was uniformly concentrated. I couldn't get anything.

As described above, a commercially available propolis extract, when poured into water, precipitates a large amount of oily components, which not only greatly impairs the image of the product, but is unsuitable as a raw material for drink products and the like. On the other hand, according to the present invention, a propolis composition such as a water-dispersible propolis extract and an emulsified propolis composition (propolis colloid) can be easily produced, and this propolis composition completely solves the conventional problems. be able to. The fact that these effects cannot be obtained by the conventional technique is apparent from the propolis compositions of Example 1 and Comparative Example 1 and the results of their production.

According to the present invention, a propolis composition having good dispersibility in water and containing a chemically synthesized product such as a polyol / fatty acid ester, unlike the prior art emulsified propolis composition of the prior art. A propolis composition having an ideal composition as a healthy food is provided.

As a method of removing ethanol from the propolis composition, there is a method of the prior patent using a polyol / fatty acid ester as a surfactant, and this method is Comparative Example 2
It cannot be applied to sugars and sugar alcohols other than glycerin as shown in. Since the method of the present invention can be applied to a propolis composition containing sugars, sugar alcohols and the like as shown in Example 5, a water-dispersible propolis composition using a wide range of materials can be obtained according to the present invention. Can be done.

The present invention is not limited to the above-mentioned embodiment. For example, the saponin is added to the propolis extract, and the mixture is stirred to extract the active ingredient in the propolis drug substance. The configuration may be arbitrarily modified and embodied without departing from the spirit of the invention.

[0041]

As described above in detail, according to the propolis composition of the first aspect of the invention, the excellent effect of exhibiting excellent dispersibility in water can be obtained based on the surface-active effect of saponin. According to the second invention, since the saponin is quillaja saponin, soybean saponin or ginseng saponin, this effect is effectively exhibited, and the excellent effect that it is suitable as a health food is obtained. Further, according to the third invention, the propolis composition containing sugar, sugar alcohol or branched cyclodextrin has an excellent effect that the stability of the aqueous dispersion obtained by the saponin surface-active action is improved. Play.

According to the method for producing a propolis composition of the fourth invention, extraction is performed by adding both the extraction solvent and the saponin having a surface-active effect, so that the extraction efficiency of the active ingredient in the propolis drug substance is improved. The excellent effect of improving is exhibited.

According to the method for producing a propolis composition of the fifth invention, the propolis composition having a low ethanol content or no ethanol content has a surfactant effect of saponin, sugar, sugar alcohol and branched cyclodextrin,
Emulsification is performed without deteriorating the active ingredient of the raw material propolis by the stabilizing action and their interaction, the concentration is performed smoothly without causing separation in the concentration process, and a high-quality and stable propolis composition is obtained. It has an excellent effect that it can be obtained in high yield.

Therefore, the propolis composition of the present invention
Since the materials used are all safe and functional materials, they are particularly useful as health foods, pharmaceuticals, cosmetics and the like.

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Claims (5)

[Claims] 1. A propolis composition comprising a propolis extract and a saponin. 2. The propolis composition according to claim 1, wherein the saponin is Quillaja saponin, soybean saponin or ginseng saponin. 3. The propolis composition according to claim 1 or 2, which contains a sugar, a sugar alcohol, or a branched cyclodextrin. 4. A method for producing a propolis composition, which comprises adding an extraction solvent and saponin to a propolis drug substance and then extracting the active ingredient in the propolis drug substance. 5. A composition containing a propolis extract and ethanol, to which saponin or saponin and sugar or sugar alcohol or branched cyclodextrin are added and concentrated, wherein the composition is low in ethanol content or free of ethanol. A method for producing a propolis composition.