JPH0859422A - Repellent for insect pest - Google Patents

Abstract

PURPOSE: To obtain a repellent for insect pests nontoxic to humans without imparting unpleasant smell and dissolving synthetic resins. CONSTITUTION: This repellent for insect pests contains a floral part of a plant of the family Oleaceae as an active ingredient. A plant of the Osmanthus, especially Osmanthus fragrans Lour. var. aurantiacus Makino is preferred as the plant of the family Oleaceae. The bloom state of a flower is not limited; however, the flower in a fully opened state full of aroma is preferably used. The floral part can directly be used or converted into small fragments for use as the repellent for the insect pests. An ingredient having repellent effects (e.g. linalool oxides or γ-decalactone) is preferably extracted from the floral part and the effective concentration is preferably raised for use. The ingredient having the repellent effects is preferably extracted by a method using an organic solvent, steam distillation or continuous extraction of the distillate obtained by the steam distillation with the organic solvent. The extraction method using the readily operable organic solvent is especially preferred.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel pest repellent which contains the flower part of the plant of the family Oleaceae as an active ingredient.

[0002]

Description of the Prior Art As a pest repellent, mosquitoes,
N, N-diethyl-m-toluamide (manufactured by Mitsubishi Gas Chemical Co., Inc., trade name: Diet), which has an effect of repelling mosquitoes such as Aedes and flies such as flies and sand flies, is known. This compound causes an unpleasant odor to humans, irritation of mucous membranes, abnormality of central nervous system, etc., so it must be handled with caution, and it is not possible to use a synthetic resin that can be easily dissolved as a container for this. Have a problem.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a pest repellent which does not give an unpleasant odor to humans, is harmless, and does not easily dissolve a synthetic resin.

[0004]

The above-mentioned object of the present invention is achieved by a pest repellent containing the flower part of the plant of the family Oleaceae as an active ingredient. Hereinafter, the present invention will be described in detail.

As a plant of the family Oleaceae, a plant of the genus Oleaceae is preferred; The flower part is composed of calyx, petals, stamens, and pistils, and the flowering condition of the flowers is not limited; it is preferable to use flowers that are sufficiently open and fragrant. As a pest repellent, the flower part can be used as it is or in the form of small fragments; from the viewpoint of space saving, a component having the repellent effect is extracted from the flower part and the effective concentration is increased before use. Is preferred.

Examples of the method for extracting a component having a repellent effect from the flower part include an extraction method using an organic solvent, a steam distillation method, a squeezing method, a headspace method, and a combination thereof. Preferred is a method capable of handling a large amount of flower parts, such as an extraction method using an organic solvent, a steam distillation method, and a method of continuously extracting a distillate obtained by a steam distillation method with an organic solvent; More preferred is an extraction method using an organic solvent that is easy to operate.

In the extraction method using an organic solvent, a component having a repellent effect can be easily extracted by immersing the flower part in the organic solvent. As the flower part, the one that has been stored (preferably one that has been sealed and stored in a cool and dark place) can be used, but it is preferable to immediately use the picked flower part in order to prevent loss of the volatile active ingredient of the flower part. .

The organic solvent is not particularly limited, and examples thereof include benzene, toluene, xylene, methylnaphthalene, petroleum ether, ligroin, pentane, chlorobenzene, dichlorobenzene, hexane, isopentane, methylene chloride, chloroform, dichloroethane, trichloro. Chlorinated or non-chlorinated aromatic, aliphatic or alicyclic hydrocarbons such as ethylene and cyclohexane; ethers such as diethyl ether, tetrahydrofuran and dioxane; ketones such as acetone and methyl ethyl ketone; methanol; , Alcohols such as ethanol, propanol, butanol, and the like; mixtures of the above solvents, and the like.

Among these solvents, it is preferable to use a solvent such as pentane, isopentane, ether, etc., from which the solvent can be easily removed. The amount of the organic solvent used is 0.1 to 100 parts by weight, preferably 2 to 20 parts by weight, based on 1 part by weight of the flower part. The dipping time of the flower part is 5 minutes to 1 month at 4 to 35 ° C. in the container (preferably a closed container is used to prevent loss of volatile active ingredient); It is 2 days to 3 weeks at 25 ° C. And although the container may be left still, it is preferable to occasionally stir it in order to enhance the extraction efficiency.

The solution containing the inflorescence extract thus obtained can be used as it is as a pest repellent; in order to enhance the repellent activity, the organic solvent is distilled off under reduced pressure. It is preferably used as a crude extract thus obtained. This crude extract is further treated by a known means such as fractional distillation, chemical treatment and various chromatographies to obtain a single active ingredient having a repellent effect against pests (for example, linalool oxide found as a substance having a repellent effect in the present invention). , Γ-decalactone, etc.) and can be obtained by separation and purification.

The pest repellent of the present invention comprises as an active ingredient a flower part of a plant of the family Amanita, an extract thereof, a separated / purified product thereof or a combination thereof. This product is prepared by preparing a composition such as cream, lotion, spray or mousse, which is prepared by adding the above substances as they are or by adding an auxiliary agent to them, and by taking a suitable coping method in consideration of the situation of repelling harmful insects. , Blood-sucking unpleasant pests [mosquitoes such as house mosquitoes (Scuttleflies mosquitoes, Culex pipiens) and mosquitoes (Yamato Aedes and Aedes albopictus),
It can be used as a repellent having a repellent effect against harmful insects such as gnats and sanitary insects (cockroaches, sand flies, house flies, fruit flies, etc.).

Examples of the auxiliary agent include surfactants, oils and fats, alcohols and the like. As a coping method, for example, application, spraying, containing, dipping and the like can be mentioned. The active ingredient concentration of the composition is usually 1 to 70% by weight for cream and 0.3 to 25 for lotion.
% By weight, usually 0.1 to 5% by weight for sprays and 0.1 to 20% by weight for mousses.

[0013]

EXAMPLES The present invention will be described in more detail with reference to examples below, but these do not limit the scope of the present invention. Example 1 [Manufacture of repellent substance] (1) Manufacture by extraction method using organic solvent The flower part (180 g) of the fully blossomed pearl oyster was dipped in a container containing isopentane (1 L) and sealed, and at room temperature. The solution was stirred for one week while rotating each container several times a day.
From the extract obtained by filtering this, the solvent was distilled off under reduced pressure to obtain 0.5 g of a crude flower extract.

(2) Manufacture by Steam Distillation Method The flower part (1 kg) of the fully blossomed pearl oyster was steam distilled for 2 hours by a conventional method. The obtained distillate was placed in a container containing isopentane (200 ml) to dissolve it, and the solubilized product obtained by filtration was concentrated to obtain 0.05 g of flower essential oil.

(3) Isolation of repellent substance The crude extract of flower part (0.1 g) obtained in (1) above was added to
Using a silica gel (Fatman) column, hexane (200 ml), benzene (200 ml), benzene / chloroform (1 / 1,200 ml), chloroform (200 ml), acetone (200 ml), and methanol (200 ml) were eluted in this order. . Each fraction was further fractionated by liquid chromatography, and the obtained active ingredient was measured by a mass spectrometer connected to a gas chromatogram. The structure was estimated from the mass spectrum data, and the standard and each data were compared to identify linalool oxides (furanoid and pyranoid) and γ-decalactone.

Example 2 [Preparation of preparation] (1) Preparation of cream 20 parts by weight of water, 7 parts by weight of Span 60 (trade name: manufactured by Kao Corporation) and 1 part by weight of carboxymethyl cellulose were uniformly mixed. Then, 40 parts by weight of the crude extract of the flower part obtained in (1) of Example 1 was gradually added thereto and stirred to obtain a cream.

(2) Preparation of lotion 70 parts by weight of water, 5 parts by weight of Span 60 (trade name: manufactured by Kao Co., Ltd.) and 15 parts by weight of Tween 60 were mixed and mixed at 60 ° C. in Example 1. A lotion was obtained by rapidly stirring while adding 10 parts by weight of the crude flower part extract obtained in (1).

(3) Preparation of Spray 10 parts by weight of the crude extract of the flower part obtained in (1) of Example 1 was dissolved in ethanol to make 35 parts by weight and the whole was filled in an aerosol container. Then, a spray was obtained by pressurizing and filling 65 parts by weight of a 1: 1 mixture of Freon 11 and Freon 12 through the valve portion.

Example 3 [Repellent efficacy test] (1) Repellent effect against flies A damp paper towel was laid on the entire bottom surface of the cage (36 cm × 21 cm × 30 cm). 20 cm of a bottle (diameter 3.5 cm × height 7.0 cm) containing a 1: 1 mixture of banana and dried yeast (5 g) was placed thereon.
Two of them were placed at an interval of 1, and one of them was used as it is as a substance shown in Table 1 [a crude extract was obtained by the organic solvent extraction method described in (1) of Example 1]. use. Commercially available products are used for other substances. ] A concentration of 10 ppm was added and mixed. Next, 50 Drosophila melanogaster (adults) were released into this cage, and 180 minutes later, the degree of repellent effect was examined. The repellent effect was examined by determining the repellent rate as shown below.

[0020]

[Equation 1]

(The number of flies in the control group indicates the number of flies collected in a bottle containing a mixture of banana and dry yeast only; the number of flies in the treated group is shown in Table 1 and banana and dry yeast. The total number of flies collected in the bottle containing the mixture of substances is shown.) The results are shown in Table 1.

[0022]

[Table 1]

(2) Repellent effect against mosquito A 0.5% ethanol solution was prepared from the crude extract obtained by the organic solvent extraction method described in (1) of Example 1. 2 μl of this solution was applied to the entire left thigh exposed by the panel.
It was applied uniformly so as to have a cm ² . On the other hand, as a control, the same amount of ethanol was applied to the same position as the left side of the right foot, and after air-drying for 5 minutes, 16 to 1 was applied to the shaded grassland where there were many mosquitoes.
The total number of mosquitoes that sucked blood was examined by staying for 2 hours until 8 o'clock. In addition, we asked the panelists to become 5 men and women aged 20 to 40. The results are shown in Table 2.

[0024]

[Table 2]

[0025]

INDUSTRIAL APPLICABILITY The pest repellent of the present invention is a blood-sucking unpleasant pest (mosquitoes such as mosquitoes and mosquitoes, gnats, etc.) and sanitary pests (cockroaches, house flies, fruit flies, etc.).
It has an excellent repellent effect against harmful insects such as. Furthermore, since this product is derived from the flower part of the plant of the family Oleaceae, it is harmless to humans and not only does not give an unpleasant odor, but rather it can positively provide a pleasant scent. Moreover, since it is difficult to dissolve the synthetic resin, it is possible to broaden the selection range of the container for storing the synthetic resin.

Claims (1)

[Claims] 1. A pest repellent comprising a flower part of a plant of the family Oleaceae as an active ingredient.