JPH08508205A - ダイエレクトロホリティクによる分離装置 - Google Patents
ダイエレクトロホリティクによる分離装置Info
- Publication number
- JPH08508205A JPH08508205A JP6521851A JP52185194A JPH08508205A JP H08508205 A JPH08508205 A JP H08508205A JP 6521851 A JP6521851 A JP 6521851A JP 52185194 A JP52185194 A JP 52185194A JP H08508205 A JPH08508205 A JP H08508205A
- Authority
- JP
- Japan
- Prior art keywords
- particles
- electrodes
- cells
- chamber
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
Landscapes
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrostatic Separation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation By Low-Temperature Treatments (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
- Sampling And Sample Adjustment (AREA)
- Hydraulic Motors (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Luminescent Compositions (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.流体から第1および第2の粒子を分離する装置において、 i)使用時に、前記流体が流過するように前記第1および第2の粒子を保持す る流体の経路内に配置される、濾過チャンバ内に配置される第1のグループの電 極と第2のグループの電極と、 ii)前記濾過チャンバが1つの入口と少なくとも1つの出口とを有し、 iii)前記第1および第2のグループの電極間にダイエレクトロホリティス( DEP)・フィールドを確立する手段と備え、 iv)前記電極間のDEPフィールドが、前記第1の粒子が拘束されるように、 合力を前記粒子が受けるようにさせ、 v)前記第2の粒子を前記チャンバから選択的に除去する手段を を備える装置。 2.ダイエレクトロホリティク・フィールドを確立して前記第2の粒子をチャン バから選択的に除去する前記手段を付勢する制御手段が設けられる請求の範囲第 1項記載の装置。 3.前記ダイエレクトロホリティク・フィールドが、一定の周波数の信号を印加 することにより変化させられる請求の範囲第1項記載の装置。 4.少なくとも1つの弁が前記濾過チャンバの各出口に配置される請求の範囲第 1項記載の装置。 5.圧力源を備える請求の範囲第1項乃至第4項のいずれかに記載の装置。 6.前記圧力源がポンプである請求の範囲第5項記載の装置。 7.前記圧力源が重力送りである請求の範囲第5項記載の装置。 8.前記制御手段が、電場を付勢することが可能なマイクロプロセッサと、弁と 、同期する前記圧力源とを含む請求の範囲第2項記載の装置。 9.前記制御手段が、電極のサブグループを周期的に切換えるように構成される 請求の範囲第2項記載の装置。 10.電位差を隣接する電極間で変化させる手段が設けられる請求の範囲第1項 記載の装置。 11.隣接する電極間に印加される電圧の周波数を変化させる手段が設けられる 請求の範囲第1項記載の装置。 12.前記切換え手段が周波数発生器を含む請求の範囲第9項乃至第11項のい ずれかに記載の装置。 13.前記圧力源が、前記第1のグループの電極が拘束される瞬間に付勢される 請求の範囲第5項記載の装置。 14.前記第1の粒子を前記チャンバから選択的に除去する手段が設けられ、該 手段が前記第2の粒子の拘束時に前記第1の粒子を前記チャンバから第2の出口 を介して圧送するよう構成される請求の範囲第1項記載の装置。 15.前記第2の粒子が前記第1の粒子より大きな速度でチャンバを通過し、第 1および第2の両粒子が異なる時点において前記チャンバから同じ出口を介して 排出される請求の範囲第7項記載の装置。 16.前記電極が城壁状のインターディジタル構造電極である請求の範囲第1項 乃至第15項のいずれかに記載の装置。 17.死んだ細胞質から生きた細胞質を分離するよう構成される請求の範囲第1 項乃至第16項のいずれかに記載の装置。 18.極性を変化させるよう制御される電気エネルギ源に接続される電気接点と 、前記濾過チャンバ内で使用される表面とを備える請求の範囲第1項記載の装置 で使用される電極。 19.電極が化学反応を強化/禁止する物質で被覆される請求の範囲第1項乃至 第18項のいずれかに記載の装置。 20.第1および第2のタイプの粒子を流体から選択的に分離する方法において 、 i)前記粒子を含む流体を少なくとも2つの電極の表面上に流過させるステッ プと、 ii)電極間に確立されたダイエレクトロホリティク・フィールドが、前記第1 のタイプの粒子を前記第2のタイプの粒子より多量の広さで拘束することができ るように電極を配置するステップと、これにより iii)前記第2のタイプの粒子が前記第1のタイプの粒子から分離させられる ように、前記第2のタイプの粒子を前記第1のタイプの粒子に対して運動させあ るいは運動を許容するステップと を含む方法。 21.少なくとも1つの圧力源が、少なくとも1つのタイプの粒子を押圧して他 のタイプの粒子に対して運動させるように構成される請求の範囲第20項記載の 方法。 22.分離を強化するため、粒子がチャンバに流入する時またはその前に標識が 付される請求の範囲第20項記載の方法。 23.前記粒子の標識付けのため金が用いられる請求の範囲第22項記載の方法 。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9306729.6 | 1993-03-31 | ||
GB939306729A GB9306729D0 (en) | 1993-03-31 | 1993-03-31 | Improvements in separators |
PCT/GB1994/000702 WO1994022583A1 (en) | 1993-03-31 | 1994-03-31 | Apparatus for separating by dielectrophoresis |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH08508205A true JPH08508205A (ja) | 1996-09-03 |
JP3586279B2 JP3586279B2 (ja) | 2004-11-10 |
Family
ID=10733088
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52185194A Expired - Fee Related JP3586279B2 (ja) | 1993-03-31 | 1994-03-31 | ダイエレクトロホリティクによる分離装置及び分離方法 |
Country Status (11)
Country | Link |
---|---|
US (1) | US5814200A (ja) |
EP (1) | EP0691891B1 (ja) |
JP (1) | JP3586279B2 (ja) |
AT (1) | ATE163576T1 (ja) |
AU (1) | AU6382894A (ja) |
CA (1) | CA2159342C (ja) |
DE (1) | DE69408830T2 (ja) |
GB (1) | GB9306729D0 (ja) |
IL (1) | IL109180A0 (ja) |
WO (1) | WO1994022583A1 (ja) |
ZA (1) | ZA942320B (ja) |
Cited By (4)
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JP2012143231A (ja) * | 2010-12-22 | 2012-08-02 | Toyama Univ | 非球体細胞の生死活性判定方法及び判定装置 |
WO2019239780A1 (ja) * | 2018-06-15 | 2019-12-19 | 富士フイルム株式会社 | 細胞培養方法、細胞培養装置、及び生産物の製造方法 |
WO2022196264A1 (ja) * | 2021-03-18 | 2022-09-22 | パナソニックIpマネジメント株式会社 | 検出方法及び検出装置 |
WO2022224846A1 (ja) * | 2021-04-23 | 2022-10-27 | パナソニックIpマネジメント株式会社 | 検出方法及び検出システム |
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1993
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1994
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- 1994-03-31 US US08/530,131 patent/US5814200A/en not_active Expired - Lifetime
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2012143231A (ja) * | 2010-12-22 | 2012-08-02 | Toyama Univ | 非球体細胞の生死活性判定方法及び判定装置 |
WO2019239780A1 (ja) * | 2018-06-15 | 2019-12-19 | 富士フイルム株式会社 | 細胞培養方法、細胞培養装置、及び生産物の製造方法 |
WO2022196264A1 (ja) * | 2021-03-18 | 2022-09-22 | パナソニックIpマネジメント株式会社 | 検出方法及び検出装置 |
WO2022224846A1 (ja) * | 2021-04-23 | 2022-10-27 | パナソニックIpマネジメント株式会社 | 検出方法及び検出システム |
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WO1994022583A1 (en) | 1994-10-13 |
DE69408830T2 (de) | 1998-06-18 |
EP0691891B1 (en) | 1998-03-04 |
US5814200A (en) | 1998-09-29 |
ATE163576T1 (de) | 1998-03-15 |
GB9306729D0 (en) | 1993-05-26 |
AU6382894A (en) | 1994-10-24 |
CA2159342A1 (en) | 1994-10-13 |
CA2159342C (en) | 2003-03-25 |
JP3586279B2 (ja) | 2004-11-10 |
EP0691891A1 (en) | 1996-01-17 |
DE69408830D1 (de) | 1998-04-09 |
IL109180A0 (en) | 1994-06-24 |
ZA942320B (en) | 1995-10-02 |
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