JPH08333266A - Medicine containing plant active ingredient and its use - Google Patents

Abstract

PURPOSE: To obtain an medicine containing the active ingredient of Angelica pubescens Maxim. and to provide the use of the active ingredient. CONSTITUTION: This medicine is an antiallergic agent containing the active ingredient of Angelica pubescens Maxim. The active ingredient is effective for activating, revitalizing, or promoting the revitalization of, the cells of the skin, internal organs etc., thus being useful for medicines, quasi-drugs, cosmetics, health foods, etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a medicine containing an active ingredient of syrup and its use.

[0002]

[Prior Art] Shishiudo is a large perennial plant that grows in the mountains and fields and grows up to 1 to 3 meters in temperate and temperate regions such as Honshu, Shikoku, and Kyushu. It has thick stems, thin leaves, and back leaves. Light green and usually hairy. (Angelica)
pubescens Maxim., A MatsumuraYabe, A. polyclada F
ranch., A. myriostachys Koidz), and is also called Takaoka Katsu, Udonashi, and Miyamashiudo. White or yellow flowers open in August-October, inflorescence branches 20-25
Book, 6-10mm long fruit is oval with dents at both ends,
The number of chromosomes is 2n = 22. The present invention is based on the finding that the active ingredient of syrup is effective in activating cells and promoting resuscitation and can be used as an antiallergic agent.

[0003]

SUMMARY OF THE INVENTION It is therefore an object of the present invention to provide a medicament containing the active ingredient of syrup and its use.

[0004]

The first aspect of the present invention is an anti-allergic agent containing an active ingredient of syrup, preferably an anti-allergic agent used for treating pollinosis and atopic dermatitis. The present invention further comprises a bactericidal agent, an antibacterial agent, an anti-inflammatory agent, a cell activating agent, a bath agent, a cosmetic product, which is an active ingredient of shishido.
It can be applied to detergents such as soap and foods.

The active ingredient has an action of activating cells and promoting resuscitation, or activating skin and promoting regeneration. The active ingredient to be used in the present invention can be used by sushid containing it in the air or after drying, cutting or crushing. Alternatively, it can be used after being extracted with a solvent such as water or ethanol. In the case of solvent extraction, the ratio of the raw material to the solvent is not particularly limited, and it is sufficient that it is usually used for extraction of plants, and the extraction time is several tens of minutes to several hours. This extract can be used on its side, or after further concentration, or by diluting this concentrate with a solvent, usually, a single dose is 10 to 1,000 mg when directly used. When diluted, it can be diluted up to 100 times.

The obtained active ingredient can be used alone or in combination with other ingredients, for example, as a composition for external or internal medicine, or as an additive for beverages and foods, and for quasi drugs, for example. It can be contained in cosmetics, detergents such as soaps, shampoos and rinses, bath agents and the like. This active ingredient activates cells such as skin and internal organs,
It has antipruritic, antipruritic and antiphlogistic effects as well as promoting regeneration and resuscitation, so it can be used as an anti-allergic agent, bactericidal agent, antibacterial agent, anti-inflammatory agent, cell activating agent, allergic dermatitis, especially hay fever and atopic. It can be used for the prevention and treatment of dermatitis, other dermatitis, eczema, acne rash, hemorrhoids, rhinitis, athlete's foot, ulcer and the like.

[0007]

INDUSTRIAL APPLICABILITY The antiallergic agent containing the active ingredient of sushido of the present invention can be used for the prevention and treatment of various allergic diseases, especially hay fever and atopic dermatitis. Since this active ingredient has an effect of activating cells such as skin and internal organs, promoting regeneration and resuscitation, in addition to antiallergic agents, bactericidal agents, antibacterial agents, antiinflammatory agents, cell activating agents, bath agents, cosmetics,
It can be applied to detergents, foods, etc. Although the active ingredient includes a substance having a known structural formula, it is considered that the antiallergic agent of the present invention is a combination of a known substance and an unknown substance.

[0008]

The present invention will be described below in detail with reference to examples. Example 1 The mud of the root of shishido was dropped and finely cut into 5 mm squares 1
kg and 3 liters of water were placed in a round bottom flask equipped with a reflux condenser and heated under reflux for 1 hour. After allowing to cool, the mixture was filtered at room temperature, and the filtrate was concentrated under atmospheric pressure to 0.01 liter in a water bath to obtain 12 g of concentrated liquid. This was an oily substance having a deep sepia color and a odor characteristic of shiushido. Example 2 A dried sardine root was cut into a length of 30 mm, and 200 g thereof was put together with 1 liter of ethanol in a round bottom flask equipped with a reflux condenser and heated under reflux for 1 hour. After allowing to cool, the mixture was filtered at room temperature, and the filtrate was concentrated to 0.005 liter in a hot water bath using an evaporator to obtain 6 g of a concentrated liquid. This one is
It was a dark sepia-colored oil with a sushido-specific odor.

Example 3 200 g of dried and powdered sardine root and 1 liter of water were placed in a round-bottomed flask equipped with a reflux condenser and refluxed at 1
Heated for hours. After allowing to cool, the mixture was filtered at room temperature, and the filtrate was concentrated to 0.005 liter under normal pressure in a water bath to obtain 10 g of concentrated liquid. This product was a deep sepia color and was an oily substance having a sushido-specific odor. The concentrated liquid thus obtained can be used for various purposes depending on the concentration, and the higher the concentration, the greater the effect. As a result, the efficacy of this product is a composite action of the constituent active ingredients.

Example 4 Separation of Active Ingredients Among the active ingredients, antibacterial active ingredients were separated. (1) The antibacterial activity test was carried out by TLC bioautography using Cladosporium herbarum . Cladosporium
herbarum was grown on PGA agar. A- below
The spores are suspended in the B mixture and pre-developed T
Sprayed onto LC plate. Approximately 2 this plate at 20 ℃
After stationary culture in a humid container for a day, white growth-inhibiting circles were observed. Solution A: KH ₂ PO ₄ 7g, NaH ₂ PO ₄ · 12H ₂ O 6g, KNO ₃ 4g, MgSO ₄
7H ₂ O 1 g in 1 liter B solution: glucose 300 g in 1 liter (2) Extraction and isolation and purification of antibacterial active substance 130 ml of the concentrate obtained in the same manner as in Example 1 was extracted 3 times with ethyl acetate. . The ethyl acetate layers were combined, washed once with saturated brine and dried over anhydrous sodium sulfate, and the solvent was evaporated under reduced pressure to give an oily extract. Cladosporium herba
Compound A having antibacterial activity, which is separated and purified by silica gel and reverse phase (ODS) column and thin layer chromatography using TLC bioautography of rum as an index.
Two species of B were isolated. Yields are 11.0 mg and 8.9 mg, respectively.
The former had 20 μg and the latter 200 μg against Cladosporium herbarum .

The analysis results of these compounds A and B are as follows. Compound A: White solid EI-MS m / z (%): 258 (M ⁺ , 53), 243 (M ⁺ -15,6), 227 (100). HR-MS: found 258.0859; calcd. For C ₁₅ H ₁₄ O ₄ 258.089
_{^{2. 1 H-NMRδ (CDCl 3}} ): 7.62 (1H, d, J = 9.5Hz), 7.43 (1H, d, J =
8.6Hz), 6.88 (1H, d, J = 8.6Hz), 6.23 (1H, d, J = 9.5Hz),
3.90 (3H, s), 2.28 (3H, d, J = 1.3Hz), 1.97 (3H, d, J = 9.5H
z). ¹³ C-NMR δ (CDCl ₃ ): 190.0, 160.2, 159.0, 158.1, 15
1.5, 143.0, 129.2, 125.5, 120.7, 113.8, 112.9, 107.
8, 56.4, 28.1, 21.3.IRν _max (CHCl ₃ ) cm ^-1 : 1732, 1604, 1493, 1294, 125
5, 1125, 1089. Compound B: White solid EI-MS m / z (%): 288 (M ⁺ , 46), 273 (M ⁺ -15,6), 257 (100). HR-MS: found 288.0984 ; calcd. for C ₁₆ H ₁₆ O ₅ 288.099
_{^{8. 1 H-NMRδ (CDCl 3}} ): 7.95 (1H, d, J = 9.9Hz), 6.31 (1H, br
s), 6.30 (1H, m), 6.15 (1H, d, J = 9.9Hz), 3.96 (3H, s), 3.
90 (3H, s), 2.25 (3H, d, J = 1.1Hz). 1.95 (3H, d, J = 1.1Hz). ¹³ C-NMR δ (CDCl ₃ ): 189.8, 160.5, 160.4, 157.5, 15
7.0, 152.8, 138.2, 126.1, 113.7, 111.6, 103.5, 90.
4, 56.3, 56.0, 28.0, 21.2 IRν _max (CHCl ₃ ) cm ^-1 : 1724, 1596, 1465, 1335, 125
5, 1144, 1115.

Compound A is a perennial plant of the Apiaceae family, Ligusticum hultenii FERNALD (L. scoticum LINN.).
It was the same substance extracted from (Hata K., Koz
awaM., and Baba K., Yakugaku Zassi, 93, 248 (1973)
. Compound B was glablactone (Glabractone (Angelicone)), whose structure was isolated from syrup (Fuj
ita M., and Furuya T., Yakugaku Zassi, 76 , 538 (19
56)).

Application Example 1 Antiallergic Agent (1) A 12-year-old child with atopic dermatitis was applied with the concentrated extract obtained in Example 2 on gauze and was applied to it.
The itch disappeared after a minute, and when it was applied twice a day by rubbing directly on the Casabta-like affected area, the symptoms were alleviated after 2 days. (2) For a 45-year-old man with allergic rhinitis, the concentrated extract obtained in Example 3 was diluted 100 times with water, and was taken 3 times a day.
The nasal cavity was sprayed and the throat was gargled twice in the morning and evening.
The symptoms of itching and sneezing disappeared after 2 days. (3) A 16-year-old girl drank the concentrated extract obtained in Example 1 10-fold with water 30 g twice a day, and healed asthma in one month.

Application Example 2 Cell activating agent The concentrated extract obtained in Example 1 was directly applied to the bilan symptom of the foot of a 50-year-old woman who had been treated for 5 years without going to the hospital, but was not cured, three times daily for two weeks. did. After 2 weeks, the inflammation disappeared and the skin started to regenerate and healed. Application Example 3 Bactericidal agent, antibacterial agent, anti-inflammatory agent The active ingredients obtained in the examples are used to reduce inflammation caused by gargling during tonsillitis, diaper rash of babies and bedridden elderly people, and reduction of itching caused by heat and rash. It was effective in healing, reducing the pain of hemorrhoids, and healing inflammation caused by insect bites.

Application Example 4 Cosmetics, Detergents The cream containing the active ingredient obtained in the examples, shampoo and rinse for hair washing, and soap were produced by a conventional method. It was effective for maintenance of skin after shaving, prevention of housewife's rough hands by detergent, prevention of dandruff and itching of hair, hair growth, hair growth, etc.

Application Example 5 Food The active ingredient obtained in the example was mixed with drinking water and health food by a conventional method. Recovery of fatigue and enhancement of energy by drinking
It was also effective in improving bowel movements and preventing obesity. A 50-year-old man with gastric weakness drank 30 g of the concentrated extract obtained in Example 1 diluted with water 5 times a day.
After a day, my appetite increased, my complexion improved, and my heartburn and stomach pain disappeared. Cut dried sardines to 50g
Was put into tea at a rate of 1000cc of hot water and was drunk by a 65-year-old woman. After 20 days, my appetite improved and bowel movements improved. In addition, the luster of the skin became better and moisturized. The itching peculiar to senileity subsided.

Application Example 6 Bath Agent A dry agent of 100% of the dried product of cinnamon was cut and put in a bag to prepare a bath agent containing an active ingredient. After one use, the skin became smooth and smooth, moisturized, and the effect of preventing rough skin was recognized. In addition, the use of a 12-year-old boy with atopic dermatitis for 7 days showed relief of the symptoms.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display location A61K 7/50 ADZ A61K 7/50 ADZ C11D 3/382 C11D 3/382 9/38 9/38 / / C07G 17/00 C07G 17/00 Z (72) Inventor Yuko Yoshizawa 1-8-711 Aioi-cho, Eniwa-shi, Hokkaido

Claims (11)

[Claims] 1. An anti-allergic agent containing an active ingredient of shishido. 2. The antiallergic agent according to claim 1, which is used for treating pollinosis. 3. The antiallergic agent according to claim 1, which is used for the treatment of atopic dermatitis. 4. A bactericide containing an active ingredient of shishido. 5. An antibacterial agent containing an active ingredient of shishido. 6. An anti-inflammatory agent containing the active ingredient of syrup. 7. A cell activator containing an active ingredient of shishido. 8. A bath preparation containing an active ingredient of syrup. 9. A cosmetic product containing the active ingredient of shishido. 10. A cleaning agent containing an active ingredient of shishido. 11. A food product containing the active ingredient of shishido.