JPH08233827A - Automatic analyser - Google Patents

Abstract

PURPOSE: To properly stirr a sample to be inspected and a reagent without exerting hindrance on the measurement of component quantity. CONSTITUTION: The analyser is provided with a power supply part 71 driving stirrer 31, a stirrer up-and-down movement mechanism part 72, a stirrer vibration frequency control part 73 controlling the frequency of the power supplied from the power supply part 71 to the stirrer 31, a stirrer driving voltage control part 74 controlling the voltage of the power supplied from the power supply part 71 to the stirrer 31 and a stirrer up-and-down movement control part 75 controlling the falling position of the stirrer 31 by the stirrer up-and-down movement mechanism part 72. On the basis of the stirring data inputted by an operation part or the stirring condition read from the liquidity-frequency setting area, liquidity-voltage setting area and liquid amt. height-position setting area of an RAM, the stirring operation specification of the stirrer 31 is altered and adjusted.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic analyzer for dispensing a test sample and a reagent into a reaction cell, stirring the mixture, and measuring the amount of a desired component from the stirred solution.

[0002]

2. Description of the Related Art An automatic analyzer corresponds to a reagent by dispensing a test sample and a reagent into a reaction cell and measuring a reaction change (change in absorptance) between the test sample and the reagent. The amount of the component to be measured is measured. At this time, in order to make the reaction between the test sample and the reagent uniform, a stirring mechanism is indispensable for stirring the test sample and the reagent.

In a conventional automatic analyzer, a rod-shaped member (hereinafter referred to as a stirrer) for stirring is inserted into a mixed liquid of a sample to be tested and a reagent in a reaction cell, and constant operating conditions (
It was general to stir by vibrating the stirrer at the frequency and amplitude).

Known methods for vibrating the stirrer include a method of vibrating the stirrer with a motor and a method of vibrating the rod-shaped stirrer itself by applying a piezoelectric element.
FIG. 9 is a diagram showing three types of general stirring bars.
) Is a screw stirrer 1, FIG. 9 (b) is a flat plate stirrer 2,
FIG. 9 (c) shows the piezo stirrer 3. In addition, 4 is a reaction cell in which a test sample and a reagent are dispensed.

Although the screw stirrer 1 and the flat plate stirrer 2 have different tip shapes, they are rotated by a rotary drive device (not shown) such as a motor to perform a stirring operation. Reference numeral 3 indicates a vibrating and agitating operation due to expansion and contraction of the piezoelectric element.

The mixed liquid to be stirred (mixed liquid of test sample and reagent) has various physical properties, and the liquid amount may be large or small. For example, there is a mixed liquid having a strong viscosity and a large amount of liquid, and the operating conditions for stirring are generally set to be slightly higher so that the mixed liquid that can be a measurement object can be sufficiently stirred. The agitated mixed solution in the reaction cell is irradiated with light in the photometry section, and the change in the absorptivity of the transmitted light is measured to calculate the desired component amount.

[0007]

Here, the effect of stirring in the case of the screw stirrer 1 and the flat plate stirrer 2 will be described with reference to experiments conducted under stirring conditions such as liquidity. 1.5% polyvinyl alcohol as a reagent
(PVA) -glycerin solution with glycerin concentration of 0,1
Table 1 shows the viscosity and the specific gravity at 0, 20, 30, 40%.

[0008]

[Table 1]

FIG. 10 is a diagram showing the relationship between the stirring time in the screw stirrer 1 at 4680 rpm and the simultaneous reproducibility CV% for each glycerin concentration of 400 μl of 1.5% polyvinyl alcohol-glycerin solution.

FIG. 11 is a diagram showing the stirring time and the simultaneous reproducibility CV% in the flat plate stirrer 2 at 4680 rpm for each glycerin concentration of 400 μl of a 1.5% polyvinyl alcohol-glycerin solution.

In any case, the simultaneous reproducibility becomes worse as the stirring time becomes shorter, and the glycerin concentration becomes higher and the viscosity becomes higher. Further, it is shown that the plate stirrer 2 has better simultaneous reproducibility than the screw stirrer 1 and that the stirring effect differs depending on the type of the stirrer.

FIG. 12 shows the amounts of each 1.5% polyvinyl alcohol-20% glycerin solution 250, 300, 35.
It is a figure which shows the relationship between the stirring time and the simultaneous reproducibility CV% in the screw stirrer 1 of 4680 rpm with respect to 0,400 microliter.

FIG. 13 shows 4680 rpm for each liquid volume of a 1.5% polyvinyl alcohol-20% glycerin solution.
It is a figure which shows the relationship between the stirring time and the simultaneous reproducibility CV% in the flat plate stirrer 1.

In any case, the simultaneous reproducibility becomes worse as the liquid volume increases. Even in this case, the plate stirrer 2 has better simultaneous reproducibility than the screw stirrer 1.

FIG. 14 shows the relationship between the stirring time and the reproducibility CV% in the screw stirrer 1 at 4680 rpm for each glycerin concentration of 20, 30 and 40% of 300 μl of 1.5% polyvinyl alcohol-20% glycerin solution. FIG.

According to FIG. 14, in the case of the solution of 300 μl, the screw stirrer 1 can relatively well stir even if the glycerin concentration of the solution is changed. The screw stirrer 1 and the flat plate stirrer 2 are rotated by a motor.
The relationship with (rotational speed) is shown in Table 2.

[0017]

[Table 2]

FIG. 15 shows the stirring time and the reproducibility CV% in the screw stirrer 1 for each rotation number of the motor of 4680, 7380, 10015 rpm in 400 μl of 1.5% polyvinyl alcohol-20% glycerin solution.
It is a figure which shows the relationship with.

FIG. 16 shows the relationship between the stirring time and the reproducibility CV% in the flat plate stirrer 2 with respect to each rotation number of the motor of 4680, 7380 and 10015 rpm in 400 μl of 1.5% polyvinyl alcohol-20% glycerin solution. FIG.

In the case of the screw stirrer 1, the simultaneous reproducibility is improved by increasing the rotation speed, but in the case of the flat plate stirrer 2, the simultaneous reproducibility is improved even if the rotation speed is increased. I can't confirm.

FIG. 17 shows that each glycerin concentration of 400 μl of a 1.5% polyvinyl alcohol-glycerin solution was 0,
Motor rotation speed 4 against 10, 20, 30 and 40%
It is a figure which shows the relationship between the stirring time and simultaneous reproducibility CV% in the screw stirrer 1 of 680 rpm and 7380 rpm.

According to FIG. 17, the screw stirrer 1
By increasing the rotation speed of the motor from 4680 rpm to 7380 rpm, good simultaneous reproducibility can be obtained even if the glycerin concentration is high and the viscosity is high.

FIG. 18 is a graph showing the relationship between the stirring time and the measurement reproducibility CV% in the screw stirrer 1 having a motor rotation speed of 4680 rpm for the case where foaming was performed with 400 μl of a 1.5% polyvinyl alcohol-20% glycerin solution and the case where no foaming was performed. FIG.

According to FIG. 18, the measurement reproducibility is poor with foaming, and the measurement reproducibility is good with foaming. FIG. 19 is a diagram showing the relationship between the operating frequency (vibration frequency) and the measurement reproducibility CV% in the piezo stirrer 3 for 400 μl of a foamable reagent and a non-foamable reagent.

FIG. 19 shows that the measurement reproducibility differs depending on the foamable reagent, the non-foamable reagent, and the operating frequency. As mentioned above, the stirring effect differs depending on the type, foaming property, viscosity, properties and amount of the mixed liquid to be stirred, and when excessive stirring occurs, bubbles are generated or the mixed liquid scatters. There was a problem such as doing.

When bubbles are generated, the photometric unit cannot accurately measure the absorptivity, and when the second reagent is dispensed thereafter, it interferes with stirring and becomes unsuitable for measuring the change in the absorptance. was there. Further, even if the mixed solution is scattered, there is a problem that the amount of the mixed solution is insufficient and it becomes unsuitable for measuring the change in absorptance.

Of course, if the stirring is not sufficiently performed, the reaction between the test sample and the reagent is measured, so that there is a problem that the accurate component measurement cannot be performed. Therefore, it is an object of the present invention to provide an automatic analyzer that can appropriately stir a test sample and a reagent without causing any trouble in measuring the amount of components.

[0028]

The invention according to claim 1 is
A test sample and a reagent are dispensed into a reaction vessel and stirred, and in an automatic analyzer that measures the amount of a desired component from the stirred solution, a stirrer that stirs the dispensed solution in the reaction vessel,
The driving means for driving the stirring bar and the operation specification changing / adjusting means for changing / adjusting the stirring operation specification of the stirring bar by the driving means are provided.

The invention according to claim 2 is an automatic analyzer for dispensing a test sample and a reagent into a reaction container, stirring the mixture, and measuring a desired component amount from the stirred solution. An agitator for agitating the injection solution, a drive means for driving the agitator, and an operation control means for changing / adjusting the agitating operation specification of the agitator by the drive means based on preset agitation conditions are provided. It is a thing.

The invention corresponding to claim 3 is an automatic analyzer for dispensing a test sample and a reagent into a reaction container, stirring the mixture, and measuring a desired component amount from the stirred solution. The stirrer that stirs the injection solution, the drive unit that drives this stirrer, and the stirring operation specifications of the stirrer that is driven by the drive unit based on the liquid information of the mixture of the sample to be tested and the reagent obtained from the stirrer. An operation control means for changing and adjusting is provided.

[0031]

In the invention according to claim 1, the operation specification changing / adjusting means changes / adjusts the operation specification of the stirrer by the driving means. Therefore, the operation specification of the stirring bar is changed / adjusted to the desired operation specification.

According to the second aspect of the invention, the operation control means changes and adjusts the stirring operation specification of the stirrer based on preset stirring conditions. Therefore, based on preset stirring conditions, such as the size of the stirrer, the amount of the test sample and the reagent, the viscosity of the mixed solution of the test sample and the reagent, etc., the operating specifications of the stirrer are It is automatically changed and adjusted to the optimum operation specifications.

In the invention according to claim 3, the operation control means changes the stirring operation specification of the stirring bar based on the liquidity information of the mixed liquid of the sample to be tested and the reagent obtained from the stirring bar.
Adjusted. Therefore, since it is based on the liquid information of the actual mixed liquid, the operating specifications of the stirring bar are automatically changed / adjusted in conformity with the actual mixed liquid.

[0034]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A first embodiment of the present invention will be described below with reference to FIGS.
Will be described with reference to. FIG. 1 is a diagram showing a schematic configuration of an analysis unit of an automatic analysis device to which the present invention is applied.

The disk-shaped reaction disk 11 in which a plurality of reaction cells are arranged on the circumference has a predetermined angle (about 90- (α / 4) ° α is one reaction cell at a certain cycle. The intermittent rotation operation is performed in which the rotation is stopped only by the angle () at which it is stored. A sample disc 12 in which a sample cup (or a blood collection tube, not shown) containing a test sample is set
Are arranged in the vicinity of the reaction disk 11 at a predetermined interval.

A first reagent container 13 in which a reagent container accommodating a reagent that reacts with various components is set is arranged inside the reaction disk 11, and like the first reagent container 13, a reagent container is provided. The second reagent storage 14 to be set is arranged in the vicinity of the reaction disk 11 at a predetermined interval.

The sample disk 12 and the first reagent storage 1
3 and the second reagent storage 14 are designated sample cups (collecting tubes) set on the sample disk 12 or designated on the first reagent storage 13 and the second reagent storage 14 by predetermined designation control. The reagent container is rotated so as to be positioned at a predetermined position.

A sample arm 15 is arranged between the reaction disk 11 and the sample disk 12, and a sample nozzle is attached to the tip thereof. The sample arm 15 positions its sample nozzle on the sample cup set at a predetermined position of the sample disk 12, and sucks a predetermined amount of sample (test sample) in the sample cup, When the step ends, the sample nozzle is rotated to position the sample nozzle on the sample dispensing position of the reaction disk 11, and dispenses the sample into the reaction cell set at the sample dispensing position by a preset amount. Note.

A first portion is provided near the outer periphery of the reaction disk 11.
A reagent dispensing arm 16 is arranged, and a first reagent dispensing nozzle is attached to its tip. The first reagent dispensing arm 16 includes the first reagent dispensing nozzle as the first reagent storage 3
Is placed on the reagent container set at a predetermined position, and a predetermined amount of the reagent in the reagent container is sucked, and when the suction is completed, the reagent container is rotated to move the first reagent dispensing nozzle to the reaction disk 11 Is placed on the first reagent dispensing position, and the reagent is dispensed in a preset amount into the reaction cell set at the first reagent dispensing position.

The reaction disk 11 and the second reagent storage 1
A second reagent dispensing arm 17 is disposed between the second reagent dispensing arm 4 and the second reagent dispensing nozzle 4, and a second reagent dispensing nozzle is attached to the tip thereof. The second reagent dispensing arm 17 positions its second reagent dispensing nozzle on a reagent container set at a predetermined position of the second reagent storage 14 and sucks a predetermined amount of the reagent in the reagent container. Then, when this suction is completed, it is rotated to position the second reagent dispensing nozzle on the second reagent dispensing position of the reaction disk 11, and the reaction set at the first reagent dispensing position. Dispense the reagent into the cell in a preset amount.

Further, a first stirring arm 18 and a second stirring arm 19 are arranged near the outer periphery of the reaction disk 11, and a stirring bar is attached to each tip.
The first stirring arm 18 and the second stirring arm 19 are configured to stir the sample in the reaction cell set at the first stirring position and the second stirring position of the reaction disk 11 by a stirrer. There is.

Further, a cleaning unit 20 is arranged near the outer periphery of the reaction disk 11, and the cleaning unit 2
At 0, a plurality of cleaning nozzles and a drying nozzle are attached. The cleaning unit 20 is configured to clean or dry each reaction cell set at the cleaning position of the reaction disk 11 by a cleaning nozzle or a drying nozzle, respectively.

Further, an electrode 21 can be optionally arranged near the outer periphery of the reaction disk 11 to analyze an electrolyte. A photometric section 22 is provided at one location on the reaction disk 11. The photometric unit 22 includes a light emitting unit, irradiates the light from the light emitting unit to the reaction cell set at the photometric position of the reaction disk 11, measures the light amount of the transmitted light, and measures the sample in the reaction cell. The amount of change due to the reagent is measured. Based on this measured variation, sample composition analysis
(Quantitative / Qualitative analysis) can be performed.

The reaction disk 11 has a constant temperature tank (constant temperature water tank) structure for maintaining the temperature of the reaction cell at a preset temperature, and the first reagent storage 13 and the second reagent storage 14 are The cooling structure keeps the temperature of the reagent container (reagent) at a preset temperature.

An example of a stirrer (bimorph type stirrer) attached to the tips of the first stirring arm 18 and the second stirring arm 19 is shown in FIGS. 2 and 3. FIGS. 2 (a) and 2 (b) are a front view and a side view showing a piezo stirrer 31 as an example, and FIGS. 2 (c) and 2 (d) show the piezo stirrer 31. It is a part of front sectional view and side sectional view.

The piezo stirrer 31 includes a blade 32 that is inserted into a mixed liquid of a sample and a reagent and vibrates, and an end portion of the blade 32 opposite to the side where the blade is inserted into the mixed liquid (hereinafter referred to as an end portion). ) Two piezoelectric elements 33, 34 provided on both sides, a holding plate 35 and a weight 36 for fixing these piezoelectric elements 33, 34 to both sides of the end portion of the blade, and the piezoelectric elements 33, 34. The lead wires 37 and 38 connected to the electrodes and the pressing plate 35 are fixed and a case 39 covering the piezoelectric element is formed.

FIG. 3 (a), FIG. 3 (b), and FIG. 3 (c).
[Fig. 4] is a front view, a side view, and a bottom view showing a piezo stirrer 41 as another example. The piezo stirrer 41 is composed of a blade 42, a piezoelectric element joint portion 43 in which two piezoelectric elements are provided on both surfaces of the blade 42, and a case 44 covering the piezoelectric element joint portion 43.

FIG. 4 is a block diagram showing a circuit configuration of a main part of the analysis section of the automatic analysis device. Reference numeral 51 denotes a CPU (central processing unit) that constitutes the main body of the control unit. A ROM (read only memory) 52 in which program data for processing performed by the CPU 51 is stored, and an area for various memories used when the CPU 51 performs processing is formed R
AM (random access memory) 53, data processing device 54
And a communication interface 55 for controlling data transmission via a line, an A / D converter 56 for converting an analog signal (electrical signal) output from the photometric unit 12 into digital data, and a drive control for a mechanical unit 57 described later. A mechanical control unit 58 and an operation unit interface 60 that controls data transmission with an operation unit 59 to which various measurement command data are input are connected to the CPU 51 via a system bus 61.

FIG. 5 is a block diagram showing a memory configuration of a main part of the RAM 53. A backup power supply for supplying power in the event of a power failure is connected to the RAM 53, and the frequency of the drive power supplied to the stirrer 31 corresponding to the liquidity information of the mixed liquid of the test sample and the reagent. (Stirrer 3
Liquidity-frequency setting area 62 in which the vibration frequency 1) is set, and a liquidity-driving voltage setting area 63 in which a driving voltage applied to the stirring bar 31 is set corresponding to the liquidity.
And a liquid volume height-position setting area 64 in which the descending position of the stirrer 31 into the reaction cell is set corresponding to the height of the mixed liquid contained in the reaction cell. .

The liquid-frequency setting area 62, the liquid-driving voltage setting area 63, and the liquid height-position setting area 64 are setting areas for the bimorph-type stirrer 3, respectively, although not shown. Similar setting areas are provided for the screw stirrer 1 and the flat plate stirrer 2, respectively. When the screw stirrer 1 is used, the setting area for the screw stirrer 1 is used. Further, when the flat plate stirrer 2 is used, the setting area for the flat plate stirrer 2 is used.

FIG. 6 is a block diagram showing the main configuration of the mechanical section 57 and the mechanical control section 58. The mechanical disc 57 includes the reaction disc 11
And various driving mechanisms such as pumps (not shown) for dispensing test samples and reagents, and various power supplies to the stirring bar 31. A stirrer power supply unit 71 and a stirrer vertical movement mechanism unit 72 for lowering and raising the stirrer 31 (the first stirring arm 8 and the second stirring arm 9) to the reaction cell are provided.

The mechanical control unit 58 is composed of a control unit for controlling the various driving mechanisms, and a stirrer vibration for controlling the frequency of the electric power supplied from the stirrer power source unit 71 to the stirrer 31. Frequency control unit 73, stirrer drive voltage control unit 7 for controlling the voltage (drive voltage) of the electric power supplied from the power supply unit for stirrer 71 to the stirrer 31.
4 and an agitator vertical movement control unit 75 that controls the agitator vertical movement mechanism unit 72.

In this embodiment having such a configuration, the operating portion 59 is used to oscillate the vibration frequency of the stirrer 31, the driving voltage to be applied, the height to be inserted into the reaction cell, etc. (or
When the stirring data such as the type of stirrer, the foamability of the solution of the test sample and the reagent, the viscosity, the liquid amount, the liquid height, etc. is input, the stirrer vibration frequency control unit 73 And the stirrer power supply unit 7 via the stirrer drive voltage control unit 74.
1 is controlled, and the stirring bar vertical movement mechanism section 72 is controlled via the stirring bar vertical movement control section 75.

FIG. 7 shows that when a stirring data is not input by the operation unit 59, a predetermined reaction cell is moved to the stirring position (first
Agitating position, second agitating position), when the solution of the test sample and the reagent contained in the reaction cell is
It is a figure which shows the flow of the stirring process which the said CPU51 performs.

The processing of step 1 (ST1) is performed by the RA
The liquid property of M53-frequency setting area 62, the liquid property-
From the drive voltage setting area 63 and the liquid amount height-position setting area 64, each information of the frequency of the drive power, the drive voltage, and the descending position (hereinafter referred to as stirring condition) is read.

The processing of step 2 (ST2) is based on the read stirring conditions, and the stirring bar vibration frequency control unit 73,
Stirrer drive voltage controller 74 and stirrer vertical movement controller 75
The operation specifications of the stirrer 31 by the stirrer power source unit 71 and the stirrer vertical movement mechanism unit 72 are changed and adjusted via the.

In the process of step 3 (ST3), the stirring operation by the stirrer 31 is performed based on the changed and adjusted operation specifications, and the stirring process is ended.
A second embodiment of the present invention will be described with reference to FIG.

The difference between this second embodiment and the above-mentioned first embodiment is that the above-mentioned C using the sensor function of the stirring bar 31 is used.
With the flow of the stirring process of the PU 51 and the change of the stirring process, it is recommended to use, as the stirrer 31, one having two piezoelectric elements or an equivalent thereof. Therefore, a bimorph type piezo stirrer fits this recommendation and at least one piezoelectric element can be used as a sensor.

FIG. 8 is a diagram showing the flow of the stirring process performed by the CPU 51 for the solution of the test sample and the reagent contained in the reaction cell when the predetermined reaction cell is located at the stirring position. Is.

The processing of step 1 (ST1) is performed by the RA
The liquid property of M53-frequency setting area 62, the liquid property-
From the drive voltage setting area 63 and the liquid amount height-position setting area 64, each information of the frequency of the drive power, the drive voltage, and the descending position (hereinafter referred to as stirring condition) is read.

The processing of step 2 (ST2) is based on the read stirring conditions, and the stirring bar vibration frequency control unit 73,
Stirrer drive voltage controller 74 and stirrer vertical movement controller 75
Via a stirrer power source unit 71 and a stirrer vertical movement mechanism unit 72 (bimorph type piezo stirrer) 31
The operating specifications of are changed and adjusted.

In the process of step 3 (ST3), the stirring operation by the stirring bar 31 is started based on the changed and adjusted operation specifications. The process of step 4 (ST4) is a change in the force applied to one piezoelectric element (used as a sensor) of the stirrer 31 from the liquid mixture of the test sample and the reagent (hereinafter referred to as the liquid to be stirred) by the stirring operation. The electrical signal that is detected and output is captured as digital data (liquid information).

In the process of step 5 (ST5), the liquidity (viscosity, liquid volume, liquid height, etc.) of the liquid to be stirred is determined from the obtained liquidity information. The process of step 6 (ST6) determines the actual minute difference between the stirring condition obtained in step 1 described above and the liquidity determined in step 5 described above,
Based on this determination, the operating specifications of the stirring bar 31 are adjusted.

In the process of step 7 (ST7), the stirring operation is performed by the stirrer 31 based on the changed and adjusted operation specification, and the stirring process is ended. Thus, according to the first and second embodiments, the stirring bar 3
1, a stirrer power source unit 71 and a stirrer vertical movement mechanism unit 72, and a stirrer vibration frequency control unit 73 for controlling the frequency of the power supplied from the stirrer power source unit 71 to the stirrer 31.
And a stirrer drive voltage control section 74 for controlling the voltage of the electric power supplied from the stirrer power supply section 71 to the stirrer 31, and a stirrer up / down control for controlling the descending position of the stirrer 31 by the stirrer up / down moving mechanism section 72. The dynamic control unit 75 is provided, and the agitation data input by the operation unit 59 or the liquid-frequency setting area 62, the liquid-voltage setting area 63, and the liquid height-position setting area 64 of the RAM 53 are read. By changing and adjusting the stirring operation specifications of the stirrer 31 based on the stirring conditions, the stirrer 31 can be adjusted according to the liquidity of the solution of the sample and the reagent contained in the reaction cell and the type of the stirrer. It is possible to change and adjust the stirring operation specifications of. Therefore, the stirring bar 3
Since the optimum stirring operation suitable for the liquid property of the above solution can be carried out by No. 1, no bubbles are generated by stirring, the solution does not scatter, and there is no trouble in the measurement of the component, The solution of the test sample and the reagent can be sufficiently stirred.

Moreover, since the stirring effect can be enhanced, sufficient stirring can be carried out in a short time, and the time required for measurement can be shortened. Further, according to the second embodiment, a bimorph type piezo stirrer is used and one of the piezoelectric elements is used as a sensor to detect the liquidity of the actual solution. Since the stirring specifications can be changed / adjusted, the stirring element 31 can perform a more optimal stirring operation that matches the liquidity of the actual solution, so that the stirring effect can be further enhanced.
The stirring can be performed sufficiently and reliably in a shorter time.

In this embodiment, the bimorph type piezo stirrer is used, but the present invention is not limited to this, and other types of piezo stirrer are used.
It can also be applied to (one piezoelectric element) and a stirrer using a motor.

[0067]

As described above in detail, according to the present invention,
By changing and adjusting the operating specifications of the stirrer, it is possible to change and adjust the operating specifications of the stirrer based on preset stirring conditions or detected stirring conditions, which hinders the measurement of the component amount. Without causing
It is possible to provide an automatic analyzer capable of appropriately and reliably stirring a test sample and a reagent.

[Brief description of drawings]

FIG. 1 is a diagram showing a schematic configuration of an analysis unit of an automatic analyzer according to a first embodiment of the present invention.

FIG. 2 is a front view, a side view, a front sectional view, and a part of a side sectional view showing an example of a bimorph type piezo stirrer used in the automatic analyzer of the same embodiment.

FIG. 3 is a front view, a side view and a bottom view showing another example of a bimorph type piezo stirrer used in the automatic analyzer of the same embodiment.

FIG. 4 is a block diagram showing a circuit configuration of a main part of an analysis unit of the automatic analysis device according to the embodiment.

FIG. 5 is a block diagram showing a main part memory configuration of a RAM of the automatic analyzer according to the embodiment.

FIG. 6 is a block diagram showing the main configuration of a mechanical unit and a mechanical control unit of the automatic analyzer according to the embodiment.

FIG. 7 is a view showing a flow of a stirring process performed by the automatic analyzer according to the embodiment.

FIG. 8 is a diagram showing a flow of a stirring process performed by the automatic analyzer according to the second embodiment.

FIG. 9 is a view showing three general types of stirring bars.

FIG. 10: 4680 for each glycerin concentration of 400 μl of 1.5% polyvinyl alcohol-glycerin solution
The figure which shows the relationship between the stirring time and the simultaneous reproducibility CV% in the screw stirrer 1 of rpm.

FIG. 11: 4680 for each glycerin concentration of 400 μl of 1.5% polyvinyl alcohol-glycerin solution
Stirring time and simultaneous reproducibility CV in flat plate stirrer 2 at rpm
The figure showing%.

FIG. 12: Liquid amount of 1.5% polyvinyl alcohol-20% glycerin solution 250, 300, 350, 400μ
The figure which shows the relationship between the stirring time and the simultaneous reproducibility CV% in the screw stirrer 1 of 4680 rpm with respect to 1.

FIG. 13 is a diagram showing the relationship between the stirring time and the simultaneous reproducibility CV% in the flat plate stirrer 1 at 4680 rpm for each liquid amount of a 1.5% polyvinyl alcohol-20% glycerin solution.

FIG. 14: Each glycerin concentration of 300 μl of 1.5% polyvinyl alcohol-20% glycerin solution 20,3
Screw stirrer 1 at 4680 rpm for 0,40%
FIG. 6 is a diagram showing the relationship between the stirring time and the simultaneous reproducibility CV% in FIG.

FIG. 15: Each rotation number of the motor 468 in 400 μl of 1.5% polyvinyl alcohol-20% glycerin solution
The figure which shows the relationship between the stirring time and simultaneous reproducibility CV% in the screw stirrer 1 with respect to 0,7380,10015 rpm.

FIG. 16: Each rotation number of the motor 468 in 400 μl of 1.5% polyvinyl alcohol-20% glycerin solution
The figure which shows the relationship between the stirring time and the simultaneous reproducibility CV% in the flat plate stirrer 2 with respect to 0,7380,10015 rpm.

FIG. 17: Concentrations of glycerin in 400 μl of 1.5% polyvinyl alcohol-glycerin solution 0, 10, 20,
The figure which shows the relationship between the stirring time and simultaneous reproducibility CV% in the screw stirrer 1 of motor rotation speeds of 4680 rpm and 7380 rpm with respect to 30, 40%.

FIG. 18 is a diagram showing the relationship between the stirring time and the measurement reproducibility CV% in the screw stirrer 1 at the rotation speed of the motor of 4680 rpm with and without foaming with 400 μl of a 1.5% polyvinyl alcohol-20% glycerin solution. .

FIG. 19 shows the operating frequency in the piezo stirrer 3 for 400 μl of a foamable reagent and a non-foaming reagent (
The figure which shows the relationship between vibration frequency) and measurement reproducibility CV%.

[Explanation of symbols]

 18, 19 ... Stirring arm, 31, 41 ... Piezo stirrer, 33, 34 ... Piezoelectric element, 51 ... CPU, 53 ... RAM, 59 ... Operation part, 62 ... Liquid foamability-frequency setting area, 63 ... Liquid amount- Voltage setting area, 64 ... Liquid amount height-position setting area, 71 ... Stirrer power supply section, 72 ... Stirrer vertical movement mechanism section, 73 ... Stirrer vibration frequency control section, 74 ... Stirrer drive voltage control section, 75 ... Stirrer vertical movement control unit.

Claims (3)

[Claims] 1. An automatic analyzer for dispensing a test sample and a reagent into a reaction vessel, stirring the mixture, and measuring the amount of a desired component from the stirred solution. The dispensed solution in the reaction vessel is stirred. An agitator, a drive unit for driving the agitator, and a stirring operation specification of the agitator by the drive unit are changed.
An automatic analyzer characterized by being provided with an operation specification changing / adjusting means for adjustment. 2. An automatic analyzer for dispensing a test sample and a reagent into a reaction vessel, stirring the mixture, and measuring the amount of a desired component from the stirred solution, wherein the dispensing solution in the reaction vessel is stirred. An agitator, drive means for driving the agitator, and operation control means for changing / adjusting the agitating operation specification of the agitator by the aforesaid drive means based on preset agitation conditions are provided. Automatic analyzer. 3. An automatic analyzer that dispenses a test sample and a reagent into a reaction container and stirs the mixture, and measures the amount of a desired component from the stirred solution. The dispensed solution in the reaction container is stirred. A stirrer, a driving means for driving the stirrer, and a stirring operation specification of the stirrer by the driving means based on the liquid property information of the mixed liquid of the test sample and the reagent obtained from the stirrer. An automatic analyzer characterized by being provided with operation control means for changing and adjusting.