JPH0810208B2 - Biosensor and biosensor measuring device - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biosensor and a biosensor measuring device capable of quantifying a specific component in various biological samples with high accuracy, quickly and easily.

[0002]

2. Description of the Related Art In recent years, various biosensors utilizing the specific catalytic action of enzymes have been developed, and there are demands for biosensors capable of rapid and accurate measurement while attempting to apply them to clinical fields. .

Taking a glucose sensor as an example, in the present day when the number of diabetic patients is remarkably increasing, it is very difficult to centrifuge blood and measure plasma as in the conventional method for measuring and managing blood glucose level. Since a complicated procedure is required, a sensor that can measure whole blood is desired.

As a simple type, similar to the test paper used at the time of urinalysis, it changes depending on the enzyme that reacts only with sugar (glucose) on the stick-shaped support and the enzyme reaction product or the product of the enzyme reaction. There is a carrier provided with a dye. Blood is dropped on this carrier, and the change in the dye after a certain period of time is visually or optically measured, but the accuracy is low due to the large interference of colored substances in the blood.

On the other hand, there has been proposed one that can be disposable for each measurement including the electrode system. However, although it is extremely simple in measurement operation, it is extremely difficult in view of the electrode material such as platinum and the structure. It has to be expensive. Moreover, although a sputtering method, a vapor deposition method, or the like can be used as a method for manufacturing the platinum electrode, it is expensive in manufacturing.

A biosensor disclosed in Japanese Patent Application Laid-Open No. 61-294351 has been proposed as a system that can be disposable including the electrode system. As shown in FIG. 9, this biosensor includes an electrode system 30 (30 ′), 31 made of carbon or the like on an insulating substrate 37 by a method such as screen printing.
After forming (31 ′) and 32 (32 ′) and providing an insulating layer 33, the electrode system is covered with a porous body 35 carrying an oxidoreductase and an electron acceptor, and a holding frame 34 and a cover 36 are used as a whole. Are integrated.

When the sample solution is dropped on the porous body, the redox enzyme and the electron acceptor carried on the porous body are dissolved in the sample solution, and an enzyme reaction proceeds between the substrate in the sample solution and the electron acceptor. The body is reduced. After the reaction is completed, the reduced electron acceptor is electrochemically oxidized, and the concentration of the substrate in the sample solution is determined from the oxidation current value obtained at this time.

In the above measurement, a predetermined voltage is supplied to the electrode system of the sensor to measure the current value flowing between the electrodes,
The substrate concentration in the sample solution is calculated based on this signal.

[0009]

With the conventional structure as described above, a wasteful measurement operation is performed when the disposable sensor is inserted in the reverse direction or when the measurement is performed while the disposable sensor is inserted in the reverse direction. There was a chance that it would end up.

An object of the present invention is to provide a simple sensor for measuring a specific component in a biological sample such as blood simply and quickly with high accuracy, and a biosensor measuring device which is easy to handle.

[0011]

SUMMARY OF THE INVENTION The present invention is a biosensor.
Protrusions, protrusions or recesses to prevent reverse insertion on a part of the board
A position indicating section having is provided. Also, the biosensor measurement device
Support for detachably supporting the biosensor substrate
The support part is provided with the position indicating part of the biosensor.
It is configured to fit only when inserted in a fixed direction.
It Then, in this mating state, the detection means operates and drives
Operate the power supply.

[0012]

In the present invention, the reverse insertion of the sensor can be prevented with a simple structure, and an error in the insertion direction can be recognized without operating the device once.

[0013]

EXAMPLES The details of the present invention will be described below with reference to examples. 1 is an exploded perspective view of the biosensor, and FIG. 2 is an external perspective view thereof. On the substrate 1, a counter electrode 4 and a measurement electrode 5, leads 3 and 2 connected to them, and an insulating layer 6 are provided. Although not shown, a reaction layer containing an enzyme and a mediator (electron acceptor) is formed so as to cover the counter electrode and the measurement electrode. A cover 9 is fixed on the substrate 1 via a spacer 7. Reference numeral 8 denotes a sample supply hole through which a test liquid (sample) is introduced onto the counter electrode and the measurement electrode by a capillary phenomenon. When the test liquid is introduced, the internal air is exhausted through the air holes 10. Reference numeral 11 is a reverse insertion preventing projection, which can prevent the reverse insertion into the biosensor device body described below.

Further, FIG. 3 shows a state in which the sensor 29 is inserted into the connector (sensor insertion opening) 13 of the apparatus body (not shown) from the direction shown by the arrow, and the sensor can be freely attached to and detached from the apparatus body. Can be supported by. 12 in the figure
Ri switch der interlocked with the driving power source provided in the fitting portion,
When the sensor is inserted to the specified position, push it with the sensor.
The drive power is turned on under pressure .

FIG. 8 is a block diagram of the control system of the biosensor device of the present invention. The measurement procedure using this device is as follows.

First, when the sensor 29 is properly inserted into the connector of the main body, the driving power source is activated by the switch 12, the insertion is detected by the detection circuit 14, and the current / voltage conversion amplifier 16, A / D converter 17 and temperature are detected via the CPU 15. Elements such as the sensor 18 are turned on.

Next, when the test liquid is introduced into the sensor, it is detected and measurement is started. After reacting for a predetermined time, a voltage is applied between the measurement electrode and the counter electrode via the reaction voltage setting circuit 24.

The signal obtained by the measurement is converted into the density through a signal processing section composed of a CPU or the like, and the LCD display 2
It is displayed on 7.

In the figure, reference numeral 25 denotes a battery which is a driving power source of the apparatus, and the power is supplied through the voltage stabilizing circuit 23 while the battery checker 26 checks the voltage.
Further, 28 is a buzzer for notifying the progress of the measurement operation, 19 is an oscillation circuit for generating a pulse for taking the operation clock of the device,
Reference numeral 22 is a circuit for resetting the CPU when the measurement is stopped midway. Reference numeral 20 is a memory for storing correction values and the like for each device.

In the above description, the wall of the sensor insertion port of the connector has a step difference, and when the sensor is inserted in the reverse direction, the reverse insertion preventing projection comes into contact with the step difference portion, and the sensor does not reach the predetermined position. The insertion difference is understood. Further, in this case, the sensor does not press the operation switch 12, so that the device does not perform the measurement operation.

The difference in the inserting direction is, for example, the case where the front and back are opposite, or the case where an attempt is made to insert from the sample supply hole side opposite to the lead portion. In any case, by providing a protrusion or a recess on a part of the sensor component, the device can be operated only when it is inserted from a predetermined direction.

Still another embodiment is shown in FIGS. 4 and 5 and FIGS. 6 and 7, respectively. FIG. 4 shows a case where a recess 38 is provided at a corner of the sensor substrate near the leads. FIG. 5 shows a state in which the sensor is inserted into a connector provided with a fitting portion that fits in this recess.

Further, FIG. 6 shows a case where a recess 39 is provided near the center of the lead portion side of the substrate, and FIG. 7 shows a sensor in a connector having a fitting portion that fits into this recess. The inserted state is shown.

As shown in the above embodiments, the biosensor is provided with a projection or a recess, and the main body of the biosensor device is provided with a fitting portion for fitting with the projection or the recess to prevent reverse insertion. You can Further, by providing a switch for turning on the driving power source in the fitting portion, the switch can be operated only when the sensor is inserted in the correct direction.

Although the reverse insertion preventing protrusion is provided on the side portion of the sensor in the above embodiment, the same effect can be obtained by providing the protrusion on the upper surface or the lower surface of the sensor. Further, the position of the recess provided in the sensor is not limited to the part shown in the above embodiment.

[0026]

As described above, in the present invention, even if the sensor is inserted in the wrong direction, the error can be confirmed in advance with a simple structure before it operates.

[Brief description of drawings]

FIG. 1 is an exploded perspective view of a biosensor according to an embodiment of the present invention.

FIG. 2 is an external perspective view of a biosensor according to an embodiment of the present invention.

FIG. 3 is a schematic cross-sectional view of a biosensor coupling portion in the biosensor device body according to an embodiment of the present invention.

FIG. 4 is an external perspective view of a biosensor according to another embodiment of the present invention.

FIG. 5 is a schematic cross-sectional view of a biosensor coupling portion in a biosensor device body according to another embodiment of the present invention.

FIG. 6 is an external perspective view of a biosensor which is still another embodiment of the present invention.

FIG. 7 is a schematic cross-sectional view of a coupling portion between a biosensor device body and a biosensor, which is a further different embodiment of the present invention.

FIG. 8 is a block diagram of a control system of a biosensor device according to an embodiment of the present invention.

FIG. 9 is an exploded perspective view of a conventional biosensor.

[Explanation of symbols]

 1 substrate 2, 3 lead 4 counter electrode 5 measurement electrode 8 sample supply hole 11 reverse insertion prevention protrusion 12 switch 13 connector 29 sensor

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Haruhiro Tsutsumi 1006 Kadoma, Kadoma City, Osaka Prefecture Matsushita Electric Industrial Co., Ltd. (72) Minoru Fukuda 1006 Kadoma, Kadoma City, Osaka Matsushita Electric Industrial Co., Ltd. (56) References JP-A-63-138255 (JP, A) JP-A-56-107154 (JP, A)

Claims (4)

[Claims] 1. A biosensor substrate is detachably supported.
Support unit, drive power supply that supplies power to the biosensor,
And biosensor having detection means for operating the drive power supply
A biosensor combined with a
Even if there is a measuring electrode, a counter electrode, and leads connecting to them,
A part of the printed circuit board has a position indicating
However, the biosensor is the biosensor
Only supported when it is inserted in the supporting direction of the measuring device in the specified direction.
The detecting means is fitted to the holding part and in this fitted state.
A biosensor configured such that the drive power source operates by moving the. 2. A support part for detachably supporting a biosensor having a position indicating part provided on a part of a substrate having at least a measurement electrode, a counter electrode, and leads connected to the measurement electrode, and a driving power supply for supplying power to the sensor. A signal processing unit that processes a current from an electrode of the sensor, a display unit that displays an output of the signal processing unit, and a detection unit that detects whether or not the sensor is inserted in the support unit in a predetermined direction. , The detecting means detects the insertion of the sensor in a predetermined direction.
Biosensor measuring device comprising means for operating said driving power when. 3. A position indicator unit Bas biosensor is a recess formed in a portion of the substrate, the support unit according to claim 2, characterized in that a convex portion to be fitted with the concave portion of the biosensor
The biosensor measuring device described. 4. The position indicating portion of the biosensor is a protrusion formed on a side surface of a substrate, and the supporting portion contacts the protrusion when the biosensor is inserted in a predetermined direction and is inserted in the opposite direction. The biosensor measuring device according to claim 2, wherein a wall surface is formed on the end of the biosensor so as to contact the tip portion of the biosensor.