JPH0759182B2 - Food preservatives - Google Patents

Description

TECHNICAL FIELD The present invention relates to a food preservative using coral sand.

(Conventional technology and its problems) With the development of the food industry, the demands of consumers have changed, and now various kinds of foods are manufactured.

For this reason, the types and amounts of food additives used are increasing year by year in order to improve quality, mass-produce, and ensure storability.

On the other hand, cyclamate (cyclamate, cyclamate),
Food additives such as AF ₂ are highly toxic and have become a social problem, and consumers are more and more emotionally repelled by food additives, making them extremely difficult for food manufacturers.

However, food additives are indispensable in order to enhance the commercial and hygienic properties of foods or the properties of foods themselves.

In particular, preservatives that prevent the spoilage of foods by microorganisms are important for maintaining the health of consumers.

However, since generally used as preservatives are relatively toxic and it is not preferable to ingest various preservatives from various foods, the types of foods that can be used for each preservative and its addition The maximum amount is specified by the Food Sanitation Law.

Conventionally used as preservatives are benzoic acid, sorbic acid, diphenyl and the like.

However, while these preservatives enhance the storability of foods, they are highly toxic and may affect the human body.

Therefore, in recent years, foods to which no preservative is added are being touted.

Therefore, an object of the present invention is to provide a natural food preservative that is different from the above-mentioned conventional preservatives and that is not only harmful to human health but rather promotes health.

(Means for Solving the Problems) In order to achieve the above-mentioned object, the gist of the present invention is to use coral sand as a preservative for foods.

That is, as a result of various researches and studies, the inventor has found that coral sand obtained from reef-building coral skeletons and half-fossils is heat-treated and pulverized to a fine powder, which has an antibacterial effect. It was found that it can be used as a preservative in place of the conventional chemical compound preservatives. The following are the results of a request to the Chiba Prefectural College of Health for testing to prove this effect.

First, a plate of Escherichia coli was partially sprinkled with coral sand which is the object of the present invention, that is, coral sand that had been subjected to heat treatment and pulverization into fine powder, and only the portion where the coral sand was sprinkled Escherichia coli growth inhibitory effect was observed.

Next, when coral sand was sprinkled on the entire surface, the growth-inhibiting effect on Escherichia coli was apparent over the entire surface. In this test, 50 ml of culture medium for desoxycholate agar medium was poured into a 100 ml petri dish to coagulate the culture medium, and then E. coli solution (Escherichia coli 3700 / m
smear l) and then incubate for 24 hours. The desoxycholate agar medium is 10 parts by weight of peptone, lactose.
10 parts by weight, sodium chloride 5 parts by weight, sodium desoxycholate 1 part by weight, iron ammonium citrate 2 parts by weight, dipotassium phosphate 2 parts by weight, neutral head 0.
It consists of 033 parts by weight and 15 parts by weight of agar powder.

Next, about 5 g of coral sand is sprinkled almost uniformly over the entire surface of the medium in the Petri dish. At this time, the particle interval of the coral sand is about 2 mm. After that, leave at room temperature (20 ° C to 25 ° C).

After 24 hours, the degree of growth of Escherichia coli is determined by photographic determination according to the Hello test.

In addition, for a sample (Comparative Example) in which 100 ml of Petri dishes containing 50 ml of E. coli smeared medium was not added with coral sand, the E. coli after 24 hours was allowed to stand at room temperature (20 ° C to 25 ° C). The degree of proliferation is determined by photographic determination according to the Hello Test.

As a result, in the sample to which the coral sand was applied, there was no growth of Escherichia coli around the part to which the coral was applied, and the effect of inhibiting the growth could be clearly recognized.

This test was also performed on a sample to which about 10 g of coral sand was added under the same conditions.
It was found that the effect of inhibiting the growth and inhibition of E. coli was greater than that of the sample containing 5 g.

In addition, for jelly, in a comparative test between the case where heat treatment and the process of pulverizing into coral sand were added and the case where it was not added, in the comparative test, the heat treatment of coral sand and the process of pulverizing into fine powder It was confirmed that the jelly added with the one subjected to and was less likely to cause mold.

Thus, it was revealed that coral sand, which has never been considered as an antiseptic, has a clear Escherichia coli growth inhibitory effect, and that it can be used as an antiseptic for natural products.

It should be noted that although silver can be attached to the coral sand to further enhance the antiseptic effect, the coral sand of the present invention can sufficiently exhibit the effect as an antiseptic agent even if silver is not attached.

The coral sand referred to in the present invention is a coral sand obtained from a living skeleton of reef-building coral or a fossil sheep group. As shown in Table I, calcium carbonate (caCO 95%) is an important biological component. It contains essential inorganic vitamin elements such as magnesium, strontium, sodium, potassium, phosphorus and chlorine, as well as trace amounts of iron, copper, zinc, manganese, cobalt and chromium.

These elements have been accumulated and weathered by the life activity of the reef-building corals, which are coelenterates.

Therefore, unlike other food preservatives obtained by chemical treatment, it has an ecological chemical organization, is safe for the human body, and also has the effect of minerals because it contains calcium carbonate as the main component. It does not harm the human body even if it is tasted directly. Regarding the fact that it does not harm the human body, it is as shown in the attached test and inspection report of the Tokyo Food Sanitation Association.

The method for producing the preservative of the present invention will be described below.

First, the naturally-obtained coral sand is washed to remove salt, and then heat-sterilized and dried at 80 to 150 ° C and 150 to 500 at room temperature.
It is crushed into a mesh, but the crushing is -180 ° C in a nitrogen atmosphere.
It can be lyophilized at -200 ° C, or kneaded with seawater or spring water.

The fine powdery coral sand thus obtained retains its porosity and is easy to add to foods, and is therefore an optimal preservative for foods.

The effect of such a food as an antiseptic can be confirmed by the above-described determination of the growth degree of Escherichia coli (photographic determination according to the Hello test), but can also be confirmed by the following experimental method.

That is, three 300 ml Erlenmeyer flasks containing 100 ml of E. coli solution were prepared, and one of them was added with 1 g of fine powder of coral sand that passed through 90% or more of 500 mesh sieve (particle size is about 27 μm) and sampled. A sample is prepared by adding 1 g of fine powder of coral sand which passes through 90% or more of 300 mesh sieve (particle size is about 43 μm) to the other one. In addition, as a comparative sample for these samples, a sample to which coral sand is not added is prepared. Here, as the Escherichia coli solution, a culture solution of a test bacterium (Escherichia coli) that has been shake-cultured in a normal broth medium at 35 ° C for 16 to 24 hours is diluted 10-fold with the same medium, and then further sterilized with phosphate buffer. It is possible to use a product diluted 1000 times with a liquid.

Next, set the Erlenmeyer flask containing each sample on a horizontal shaker, shake them horizontally at 120 rpm at 25 ° C, and after 6 hours and 24 hours, pipette the center of the sample in the Erlenmeyer flask. Plug in and collect test water. Then, the pour plate culture method using standard agar medium from the test water (35
When the number of viable bacteria was measured by culturing at 48 ° C for 48 hours), E. coli continued to increase in the sample to which the coral sand fine powder was not added, whereas the number of E. coli was increased in the sample to which the coral sand fine powder was added. It can be confirmed that the coral sand fine powder has a bacteriostatic effect. together,
It can also be confirmed that the finer the particle size of the coral sand fine powder, the greater the antibacterial effect tends to be. Further, even if the same test as the above is performed on the sample to which 0.2 g of the coral sand fine powder is added, in the sample to which the coral sand fine powder is not added, the E. coli continues to increase, whereas the coral sand fine powder is added. In the added sample, the number of Escherichia coli can be significantly reduced, and it can be confirmed that the coral sand fine powder has an antibacterial action. In addition, it can also be confirmed that the finer the particle size of the coral sand fine powder, the greater the bacteriostatic effect tends to be.

(Effects of the Invention) As described above, the food preservative of the present invention is natural coral sand, and there is no fear of harming the human body, and since the main component of coral sand is calcium carbonate, When used as a preservative for food, it not only contributes to the effect as a preservative, but also contributes to health promotion by naturally replenishing calcium. Further, since coral sand has fine porosity even when it is pulverized into fine powder, it is eaten as it is, and thus it is suitable as a preservative for foods that need to be made into fine powder.

Claims (1)

[Claims] 1. A preservative for foods, which comprises heat treatment of coral sand and pulverization into fine powder.