JPH07284391A - Oligonucleotide for detecting human herpes virus and its use - Google Patents

Abstract

PURPOSE:To obtain an oligonucleotide useful for simple and rapid detection of human herpes virus 6 type A. CONSTITUTION:This oligonucleotide has >=70% homology to the nucleic acid sequences GTGCCTATTA TACAGTTCCAGA, GTTAATGTTT GTCGAACCGGGG or CTCTTGGGTT GTGGGGCGATTT or >=70% homology to these complementary sequences. The oligonucleotide can be prepared by a chemical synthesis.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to human herpesvirus (sometimes abbreviated as HHV) type 6 type A (HHV-6A), type B (HHV-6B) and human herpesvirus type 7 (HHV). -7) Oligonucleotides for simple and rapid detection and uses thereof.

[0002]

HHV-6 has been isolated from patients with AIDS and lymphocyte diseases in the United States and the United Kingdom since 1986. In addition, the virus causing sudden rash is also HHV-
It was proved to be 6 by the present inventors. Subsequent analysis of the genomic structure revealed that HHV-6 can be divided into two types, A and B. On the other hand, HHV-
7 is a new herpesvirus isolated for the first time in 1990, but it was shown to be related to HHV-6 by epidemiological studies and analysis of genome structure. All of these viruses are rapidly isolated from AIDS patients and immunosuppressed patients after organ transplantation, and can be used as an index for the management of immunosuppressed conditions. There is an urgent need to establish a simple and quick diagnostic method.

As a method of detecting these HHV,
A method has been reported in which lymphocytes in the blood of a patient are cultured and then reacted with antiserum to detect HHV serving as an antigen by a fluorescent antibody method. However, this method requires several days for culturing lymphocytes, and rapid diagnosis cannot be performed.
There is also a method of measuring the antibody in the patient's serum, in which case at least 1 is used until the convalescent period when the antibody is produced in the patient.
It takes a week, and I still can't make a quick diagnosis. Although the detection of HHV by a gene diagnostic method has been attempted with the recent progress of gene diagnostic techniques, information on the gene sequences of these viruses is scarce and a system capable of differential detection at the same time has not yet been reported.

[0004]

Accordingly, the object of the present invention is to provide HHV-6A, HH in a simple, rapid, specific and highly sensitive manner.
It is to provide an oligonucleotide capable of detecting V-6B, HHV-7.

[0005]

[Means for Solving the Problems] The inventors of the present invention have determined the gene sequence of HHV and, as a result of further various studies, have a sequence capable of differentially detecting HHV in a simple, rapid, specific and highly sensitive manner. The present invention was completed by obtaining an oligonucleotide, establishing a detection method using the oligonucleotide.

The present invention relates to HHV-6A, HHV-6B,
An oligonucleotide specific for HHV-7, ie,
Sequence Listing ・ Sequence Nos. 1 to 12 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position may be replaced with uracil (U)) H having 70% or more homology with a nucleic acid sequence or 70% or more homology with a sequence complementary thereto
It is an oligonucleotide for HV detection.

In the present invention, the sequence listing: SEQ ID NO: 3, 7 or 8 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)). 70% with the nucleic acid sequence shown in FIG.
An oligonucleotide for detecting human herpesvirus-6A specificity, which has the above homology or has 70% or more homology with the complementary sequence thereof.

In the present invention, the sequence listing is SEQ ID NO: 4, 9 or 10 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)). And the nucleic acid sequence shown in FIG.
An oligonucleotide for human herpesvirus-6B-specific detection, which has a homology of not less than 70% or has a homology of not less than 70% with a complementary sequence thereof.

Further, the present invention provides a sequence listing / SEQ ID NO: 5,11.
Or 70% or more with the nucleic acid sequence shown in 12 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position may be replaced with uracil (U)) Or a human herpesvirus-7-specific detection oligonucleotide having a homology of 70% or more with the complementary sequence thereof.

Further, the present invention provides the sequence listing: SEQ ID NO: 1, 2 or 6 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)). And the nucleic acid sequence shown in FIG.
Oligonucleotides for human herpesvirus-6A, 6B and 7 common detection, characterized by having a homology of at least 70% or having a homology of at least 70% with their complementary sequences.

The present invention also provides a labeled nucleic acid probe obtained by labeling the above-mentioned HHV detection oligonucleotide with D in a sample.
A method for detecting HHV in a sample, which comprises hybridizing with NA or RNA and measuring the label of the hybridized conjugate.

Further, the present invention provides a nucleic acid primer which is the above-mentioned HHV detection oligonucleotide, or a labeled nucleic acid primer obtained by labeling the oligonucleotide, in a sample.
Hybridize with NA or RNA, extend the primer,
A method for detecting HHV in a sample, which comprises measuring the obtained primer extension product.

The present invention also provides a reagent kit for detecting HHV in a sample, which comprises a labeled nucleic acid probe labeled with the above-mentioned oligonucleotide for detecting HHV.

Further, the present invention is a reagent kit for detecting HHV in a sample, which comprises a nucleic acid primer which is the above-mentioned oligonucleotide for detecting HHV, or a labeled nucleic acid primer obtained by labeling the oligonucleotide.

The HHV detection oligonucleotide of the present invention has the sequence listing: SEQ ID NOS: 1 to 12 (A is adenine,
C represents cytosine, G represents guanine, and T represents thymine. Further, T at an arbitrary position may have a homology of 70% or more with the nucleic acid sequence represented by uracil (U), or
Alternatively, it has 70% or more homology with their complementary sequences and specifically reacts with HHV. In general, since oligonucleotides can be prepared by chemical synthesis, compared to cloned oligonucleotides or polynucleotides, it is possible to obtain oligonucleotides of constant quality in a large amount and at low cost.

The oligonucleotide of the present invention may be deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). In the case of ribonucleic acid, it goes without saying that the thymidine residue (T) is read as the uridine residue (U). Alternatively, a DNA containing a uridine residue may be used by synthesizing by changing T at any position to U at the time of synthesis. Similarly, any U
It may be RNA containing a thymidine residue in which is changed to T. Further, the oligonucleotide may have a mutation such as deletion, insertion or substitution of several bases, or a modified nucleotide.

The labeled nucleic acid probe of the present invention is the above HHV
A label is bound to the detection oligonucleotide, and as the label, known labels such as radioactive substances, enzymes, fluorescent substances, luminescent substances, antigens, haptens, enzyme substrates, insoluble carriers and the like can be used. The labeling method may be end labeling or labeling in the middle of the sequence. It may also be a label on the sugar, phosphate group, or base moiety.

As the nucleic acid primer of the present invention, the above HHV detection oligonucleotide is used as it is, or the oligonucleotide is labeled.
Using the HHV detection oligonucleotide of the present invention, HH
When detecting V, there are (1) a method of detecting the oligonucleotide of the present invention as a probe, and (2) a method of performing an extension reaction with the oligonucleotide of the present invention as a primer using a DNA polymerase or the like. In the case of the above (2), detection can be easily performed by introducing a label into the above oligonucleotide.

In the case of (1) above, the labeled nucleic acid probe labeled with the oligonucleotide of the present invention is used as a DNA in a sample.
Alternatively, it can be achieved by hybridizing with RNA and detecting the label of the hybridized conjugate by an appropriate detection method. On the other hand, in the case of the above (2), the oligonucleotide of the present invention is used as a nucleic acid primer as it is, or a labeled nucleic acid primer obtained by labeling the oligonucleotide is hybridized with DNA or RNA in a sample, and the primer is treated with DNA polymerase or the like. Extend and measure the resulting primer extension directly or with a labeled probe.

When the oligonucleotide of the present invention is used as a primer, it is possible to specifically amplify only the HHV gene by carrying out gene amplification (PCR etc.) using DNA polymerase or the like. In the amplification reaction, HHV can be easily detected by a method of incorporating a labeled nucleotide such as a radioactive substance during the reaction, or by fractionating the amplification product by electrophoresis and detecting a specific band. Furthermore, by using the type-specific oligonucleotide of the present invention, the type can be easily determined by the size of the amplification product after electrophoresis. Further, the amplification product can be directly detected by using the above-mentioned labeled oligonucleotide as a primer.

The HHV detection reagent kit of the present invention comprises the HHV detection oligonucleotide of the present invention or a labeled oligonucleotide obtained by labeling this oligonucleotide with an appropriate labeling agent. The reagent kit containing the nucleic acid primer may contain a labeled probe. Further, other components include a labeled detection substance, a buffer solution and the like. Furthermore, in the reagent kit of the present invention containing a nucleic acid primer or a labeled nucleic acid primer, as other components,
Nucleic acid synthetase (DNA polymerase, RNA polymerase, reverse transcriptase, etc.), substrate (dNTP,
rNTP, etc.), a labeled detection substance, a buffer solution and the like.

Further, the oligonucleotide of the present invention can be bound to a solid phase carrier and used as a capture probe. In this case, the sandwich assay may be performed with a combination of a capture probe and a labeled (nucleic acid) probe, or there is a method of labeling and capturing a target nucleic acid. There is also a method in which an oligonucleotide is labeled with biotin, and after hybridization, it is captured with an avidin-binding carrier.
In the sandwich assay, if the oligonucleotide of the present invention is used for either one of them, specific measurement becomes possible due to the specificity of the oligonucleotide, and there is no problem even if the specificity of the other oligonucleotide is slightly low.

[0023]

EXAMPLES The effects of the present invention will be further clarified by exemplifying the examples of the present invention. Example 1 Preparation of Various Oligonucleotides Using ABI DNA Synthesizer Model 391, various oligonucleotides having the sequences shown in the sequence listing and SEQ ID NOs: 1 to 12 were synthesized by the phosphoamidite method. The oligonucleotides shown in the sequence listing and SEQ ID NOs: 1 to 12 are respectively oligonucleotides (1) to (12)
Call. The method is 0.2 μM according to the ABI company manual.
Performed on a scale. Deprotection of various oligonucleotides was carried out with ammonia water at 55 ° C. overnight. Purification was carried out by FPLC manufactured by Pharmacia on a MonoQ column. If necessary, the synthesized oligonucleotide may be Jablonski.
Alkaline phosphatase (Alp) was bound according to the method of the same group (Nucl. Acid Res. 14: 6115-6128, 1986).

Example 2 Amplification of HHV (1) Preparation of viral nucleic acid An HHV amplification reaction was carried out using the oligonucleotide of the present invention. Other hitherto known human herpesviruses were also examined simultaneously to determine their specificity. The HHV standard strains used in this experiment are shown below. -HSV-1: Seivert strain-HSV-2: UW268 strain-VZV: Kawaguchi strain-EBV: B95-8 strain-CMV: AD169 strain-HHV-6A: U1102 strain-HHV-6B: Hashimoto strain-HHV-7: RK strain The viral nucleic acid was prepared by a conventional method.

(2) Amplification of HHV nucleic acid The above nucleic acid solution or human placental DNA was added to 94 μl of the reaction solution.
5 μl of solution, oligonucleotides (1) and (6) of Example 1
Alternatively, 1 μl of the mixed solution of (3), (4), (5) and (6) was added as a primer to carry out a nucleic acid amplification reaction. The composition of the reaction solution is as follows. Reaction solution composition KCl 50 mM Tris-HCl (pH 8.3) 10 mM MgCl ₂ 1.5 mM Gelatin 0.01% dATP, dCTP, dGTP, dTTP 0.2 mM Tth DNA polymerase 40 units / ml The reaction conditions are as follows. Heat denaturation: 94 ° C., 1 minute, annealing: 55 ° C., 2 minutes Polymerization reaction: 75 ° C., 1.5 minutes 30 times.

These operations were carried out using a DNA thermal cycler manufactured by Perkin-Elmer / Cetus. The detection was carried out by subjecting 10 μl of the reaction solution to 2% agarose gel electrophoresis, staining with ethidium bromide, and then detecting fluorescence under ultraviolet light. The electrophoretic conditions were a constant voltage of 100 V and a time of 30 minutes. The procedure and other conditions are described in Maniatis et al., Molecular.
The method described in Cloning (1982) was followed. In addition to the reaction solution, a molecular weight marker was also electrophoresed at the same time, and the size of the detected amplification product was calculated by comparing the relative mobilities. As a result, the amplification product was detected only when HHV-6A, HHV-6B, or HHV-7 was used (Table 1).

[0027]

[Table 1]

Example 3 Differential Detection with HHV Probe (1) Amplification of HHV Nucleic Acid 5 μl of the HHV Nucleic Acid Solution or Human Placental DNA Solution in 94 μl of Reaction Solution, Oligonucleotide (2) of Example 1
1 μl of the mixed solution of (6) and (6) was added as a primer to carry out a nucleic acid amplification reaction. The composition of the reaction solution, the reaction conditions, etc. are shown in Example 2.
According to. (2) Dot blot. Fractional Detection by Hybridization Dot blot hybridization was performed to identify which human herpesvirus the amplification product resulting from the amplification reaction was derived from. As a probe, the oligonucleotides of (7), (8), (9), (10), (11) and (12) of Example 1 were treated with alkaline phosphatase (Al).
p) Labeled one was used. Add 100 μl of 0.3N NaOH to 1 μl of the above amplification product, and add at room temperature for 15
It was denatured for a minute and blotted on a nylon membrane (Hybond N Plus, Amersham) wetted with 5 × SSC using a dot blotter (BRL). SSC is a 15 mM trisodium citrate and 15 mM sodium chloride solution, and 5 × SSC is a 5-fold concentrated solution of SSC. Eight sheets were prepared by immobilizing nucleic acid by heating this membrane at 80 ° C. for 30 minutes, and hybridization was carried out using each Alp-labeled probe.

First, the dried film (1 × 8 cm) is treated with 5 × S.
Immerse in SC for 5 minutes. Next, the membrane was transferred to a hybridization bag (manufactured by BRL), and 5 ml of a hybridization buffer (5 × SSC, 0.5% bovine serum albumin, 0.5% polyvinylpyrrolidone, 1% sodium dodecylsulfate) was added to the policyr. Seal with
Prehybridization was performed at 50 ° C. for 15 minutes. Next, hybridization was carried out at 55 ° C. for 15 minutes with 5 ml of a hybridization buffer containing 5 μl of any of the above probes. The membrane was taken out from the polybag, and washed with Shake Solution-1 (1 × SSC, 1% sodium dodecyl sulfate) at 55 ° C. for 5 minutes with shaking twice. Further, the membrane was dried after washing with Washing solution-2 (1 × SSC) twice at room temperature for 5 minutes with shaking.

1 ml of color developing solution (200 mM Tr
is-HCl (pH 8.5), 0.58% NaC
1, 1% MgCl ₂ , 0.00136% ZnC
l ₂ , 0.02% NaN ₃ , 75 mg / ml NB
T (nitro bluetetrazolium),
50 mg / ml BCIP (5-bromo-4-c
hloro-3-indolyl phosphat
e)) was added and the color reaction was carried out at 37 ° C. for 30 minutes to detect human herpesvirus. As a result, the oligonucleotide probes specific to HHV-6A, HHV-6B, and HHV-7 specifically react with the amplification products derived from the respective viruses, and the amplification from other viruses or human placenta DNA is amplified. No reaction with the product was observed (Table 2).

[0031]

[Table 2]

[0032]

EFFECTS OF THE INVENTION According to the present invention, it has become possible to measure HHV, which has conventionally required a long time for culturing, by type, quickly, simply and specifically and with high sensitivity. The oligonucleotide of the present invention can be used both as a primer for amplification reaction and as a probe for direct detection. Also. By detecting the amplification product amplified by the primer with a probe, it is possible to detect with higher sensitivity and specificity, which is of great clinical significance.

[0033]

[Sequence list]

SEQ ID NO: 1 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..22 Method for determining features : S Other features: Sequence common to HHV-6A, HHV-6B, HHV-7. Array ATAATTGGCA ATGAACACCG TT 22

SEQ ID NO: 2 Sequence length: 24 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..24 Method determined: S Other features: Sequence common to HHV-6A, HHV-6B, HHV-7. Sequence TTTTGATATG GGTTTAGGTT TTAG 24

SEQ ID NO: 3 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..22 Features Method determined: S Other features: HHV-6A specific sequence. Sequence GTGCCTATTA TACAGTTCCA GA 22

SEQ ID NO: 4 Sequence length: 24 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..24 Method determined: S Other features: HHV-6B specific sequence. Array GTCAACAAGC TATCTGCGAA GTCG 24

SEQ ID NO: 5 Sequence length: 24 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..24 Method determined: S Other features: HHV-7 specific sequence. Array TACACCAACC CTACTGTAAA TAGT 24

SEQ ID NO: 6 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..23 Determined method: S Other characteristics: HHV-6A, HHV-6B, HHV-7C
Sequence common to MV. Sequence GATCCTTTTT GAGATGCCCA AGG 23

SEQ ID NO: 7 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1.23 Method determined: S Other features: HHV-6A specific sequence. Array GTTAATGTTT GTCGTAACCG GGG 23

SEQ ID NO: 8 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..22 Features Method determined: S Other features: HHV-6A specific sequence. Array CTCTTGGGTT GTGGGGCGAT TT 22

SEQ ID NO: 9 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..23 Method determined: S Other features: HHV-6B specific sequence. Array GTTAATATTT GTCGTGACCG GAG 23

SEQ ID NO: 10 Sequence length: 22 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..22 Features Method determined: S Other features: HHV-6B specific sequence. Sequence CTCTGGGGTT ATGGGGCAAT TT 22

SEQ ID NO: 11 Sequence length: 31 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..31 Features Method determined: S Other features: HHV-7 specific sequence. Sequence TCATTACTCC AGTGACTTCC GATATTAATT T 31

SEQ ID NO: 12 Sequence length: 30 Sequence type: Nucleic acid Number of strands: Both forms Topology: Linear Sequence type: Other nucleic acid Synthetic DNA Sequence features Location: 1..30 Method determined: S Other features: HHV-7 specific sequence. Array CTTTCGGTAA GATAAATAAG CAATCACAAT 30

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical indication location G01N 33/569 J (72) Inventor Yutaka Takarada 1-1-1 Katata, Otsu, Shiga Prefecture Toyobo Co., Ltd. Research Institute, Inc.

Claims (19)

[Claims] 1. SEQ ID NO: 1 to 12 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is replaced with uracil (U). 70% or more homology with the nucleic acid sequence shown in the above), or 70% or more homology with the complementary sequence thereof, an oligonucleotide for detecting human herpesvirus. 2. Sequence Listing: SEQ ID NO: 3, 7 or 8 (wherein A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
Which has a homology of 70% or more with the nucleic acid sequence shown in (or may be substituted with) or has a homology of 70% or more with a complementary sequence thereof, for the detection of human herpesvirus-6A specificity. Oligonucleotides. 3. Sequence Listing: SEQ ID NO: 4, 9 or 10 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
May have a 70% or higher homology with the nucleic acid sequence shown in (or may be replaced with) or a 70% or higher homology with a complementary sequence thereof. Oligonucleotides. 4. Sequence listing / SEQ ID NO: 5, 11 or 12
(However, A is adenine, C is cytosine, G is guanine,
T represents thymine. In addition, T at any position has 70% or more homology with the nucleic acid sequence shown in uracil (U), or its complementary sequence 7
An oligonucleotide for human herpesvirus-7-specific detection, which has 0% or more homology. 5. Sequence Listing: SEQ ID NO: 3, 7 or 8 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
Human herpesvirus having a homology of 70% or more with the nucleic acid sequence shown in
6A specific detection oligonucleotide. 6. Sequence Listing: SEQ ID NO: 4, 9 or 10 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
Human herpesvirus having a homology of 70% or more with the nucleic acid sequence shown in
6B Specific detection oligonucleotide. 7. Sequence listing / SEQ ID NO: 5, 11 or 12
(However, A is adenine, C is cytosine, G is guanine,
T represents thymine. In addition, T at an arbitrary position has a homology of 70% or more with the nucleic acid sequence shown in uracil (U)), and a human herpesvirus-7 specific detection oligonucleotide. 8. Sequence Listing: SEQ ID NO: 3, 7 or 8 (A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U).
Which may be replaced with). The oligonucleotide for human-herpesvirus-6A specific detection, which is 9. Sequence Listing: SEQ ID NO: 4, 9 or 10 (A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U).
Which may be replaced with). An oligonucleotide for human-herpesvirus-6B-specific detection, which is 10. Sequence listing / SEQ ID NO: 5, 11 or 12
(However, A is adenine, C is cytosine, G is guanine,
T represents thymine. In addition, T at an arbitrary position is a nucleic acid sequence represented by uracil (U)), and a human herpesvirus-7 specific detection oligonucleotide. 11. Sequence Listing: SEQ ID NO: 1, 2 or 6 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
May have a 70% or higher homology with the nucleic acid sequence shown in Table 1 or a 70% or higher homology with a complementary sequence thereof, and a human herpesvirus-6A, 6B and 7 Common detection oligonucleotides. 12. Sequence Listing: SEQ ID NO: 1, 2 or 6 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
Human herpesvirus having a homology of 70% or more with the nucleic acid sequence shown in
6A, 6 and 7 Common detection oligonucleotides. 13. Sequence Listing: SEQ ID NO: 1, 2 or 6 (where A is adenine, C is cytosine, G is guanine, T is thymine, and T at any position is uracil (U)).
Which may be substituted with), the human herpesvirus-6A, 6B and 7-specific detection oligonucleotide. 14. A human herpesvirus detection oligonucleotide according to any one of claims 1 to 13 is labeled, and the obtained labeled nucleic acid probe is used as a DNA or RNA in a sample.
A method for detecting human herpesvirus in a sample, which comprises hybridizing with and measuring the label of the hybridized conjugate. 15. A nucleic acid primer which is the oligonucleotide for detecting human herpesvirus according to any one of claims 1 to 13 or a labeled nucleic acid primer obtained by labeling the oligonucleotide is hybridized with DNA or RNA in a sample to extend the primer. A method for detecting human herpesvirus in a sample, which comprises measuring the obtained primer extension product. 16. A reagent kit for detecting human herpesvirus in a sample, which comprises a labeled nucleic acid probe labeled with the oligonucleotide for detecting human herpesvirus according to any one of claims 1 to 13. 17. A human herpesvirus in a sample, which comprises a nucleic acid primer which is the oligonucleotide for detecting human herpesvirus according to any one of claims 1 to 13 or a labeled nucleic acid primer obtained by labeling the oligonucleotide. Reagent kit for detection. 18. A labeled nucleic acid probe obtained by labeling the oligonucleotide for detecting human herpesvirus according to any one of claims 1 to 13, and a nucleic acid primer or the oligo that is the oligonucleotide for detecting human herpesvirus according to claim 1. A reagent kit for detecting human herpesvirus in a sample, which comprises a labeled nucleic acid primer labeled with a nucleotide. 19. The reagent kit for detecting human herpesvirus in a sample according to claim 16, which further contains a nucleic acid synthesizing enzyme and dNTP and / or rNTP and a buffer solution.