JPH07209305A - Full-automatic hdl-cholesterol analyser - Google Patents

Abstract

PURPOSE:To continuously perform daily routine examination by sucking a sample having undergone color reaction at a measuring position up to a flow cell to optically measure the same at a predetermined wavelength. CONSTITUTION:A filtering and reaction container 6 is intermittently transferred to the downstream part of a container transfer passage 8 to reach a sampling/ first reagent adding position (d) and a first reagent is added to the container 6. Thereafter, the container 6 reaches a second reagent adding position (e) to receive the addition of a second reagent and is stirred at a stirring position (f) by a stirrer 34. By adding the first and second reagents to a supernatant sample S2, cholesterol ester in the sample supernatant S2 is decomposed into free cholesterol and fatty acid. Cholesterol receives the action of cholesterol oxidase to be oxidized to form hydrogen peroxide which is turn receives the action of peroxidase to form a blue dye. Therefore, the color-developed sample S2 is introduced into a flow cell 11 at a measuring suction position (g) and the absorbancy thereof is measured.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a completely new fully automatic HDL-cholesterol analyzer which can perform HDL-cholesterol analysis fully automatically.

[0002]

BACKGROUND OF THE INVENTION As is well known, serum lipids are roughly classified into cholesterol, triglyceride, phospholipids, free fatty acids and the like. It exists in a soluble state in bound water.

Riboprotein is a chylomicron due to its specific gravity,
Very low density riboprotein (VLDL), low density riboprotein (LD
L), high density riboprotein (HDL), and blood HD
The measurement of L-cholesterol concentration is considered to be a clinically meaningful test in order to know arteriosclerosis, ischemic heart disease, obesity, diabetes or chronic renal failure.

By the way, as such an HDL-cholesterol measuring method, an ultracentrifugation method, a gel filtration method, an electrophoresis method and the like have been conventionally known. Although the binding precipitation method is widely used at present, it is very inefficient because the precipitation reagent and the supernatant sample are dispensed and the first and second reagents are added by hand. In addition, there is a problem that the inspection cost increases.

The present invention was devised in view of the above circumstances, and an object thereof is to use a binding precipitation method useful as a routine routine test as an HDL-cholesterol measurement method, and further It is an object of the present invention to provide a fully-automatic HDL-cholesterol analyzer capable of continuously performing this type of test by fully automating all processes required for the precipitation method.

Specifically, a fully automatic HDL according to the present invention
-A cholesterol analyzer adds a precipitation solution containing phosphotansteric acid and a magnesium salt to a sample to selectively precipitate riboproteins other than HDL, and the supernatant obtained by removing the precipitate is allowed to act with a coloring reagent to give a supernatant. Cholesterol esters therein are decomposed into free cholesterol and fatty acids by the action of cholesterol esterase. The cholesterol thus produced is oxidized by the action of cholesterol oxidase together with the existing free cholesterol, and at the same time, hydrogen peroxide is produced.
This generated hydrogen peroxide is peroxidase (PO
D) and 4 with aniline sodium (DAOS)
-Aminoantivirine is quantitatively oxidatively bonded to form a blue pigment. Therefore, the HDL-cholesterol concentration can be determined by measuring the absorbance of the blue color, and the fully automatic HDL-cholesterol analyzer according to the present invention continuously performs these operations fully automatically without any human intervention. It has the greatest feature in that it can be performed.

[0007]

In order to achieve the above object, according to the present invention, a fully automatic HDL-cholesterol analyzer is provided, in which a required amount of serum is supplied from a sample container containing serum to a reaction tube section. A sample pipette device for dispensing a sample, a filtration / reaction container body having a plurality of the above-mentioned reaction tube portion and a filtration tube housing portion, and a filtration tube from the sample discharge / precipitation reagent discharge position A cassette transfer device for sequentially transferring from the resampling position / reagent addition position to the measurement suction position via the transfer position, and the filter tube from the filter tube storage position of the filtration / reaction container body to the reaction tube at the filter tube transfer position. Means for transferring into the chamber, an optical measuring device for sucking a sample that has undergone color reaction at the measuring position to the flow cell and performing optical measurement at a predetermined wavelength, and control for driving and controlling these organically linked Is characterized in that it has configuration comprises a location, a.

In the present invention, the filtration / reaction container body is characterized in that the filtration tube housing portion and the reaction tube portion are integrally formed in series in a paired state. The bottom part of the filter tube storage part is a filter tube storage part in which the upper part of the filter tube protruding upward from the upper surface of the cassette is stacked when the above-mentioned filtration / reaction container bodies are vertically stacked with the filter tube being stored. It is characterized by being raised to a height that does not collide with the bottom of the section.

Further, in the fully-automatic HDL-cholesterol analyzer according to the present invention, at the resampling position, the filter tube inserted into the reaction tube section is gradually pressed by the pressurizing means so that the inside of the reaction tube section is reduced. The sample that has undergone precipitation reaction is filtered, and at the resampling position, the supernatant sample filtered by the filter tube inserted into the reaction tube section is discharged together with the first reagent into the filter tube storage section. It is characterized in that they are reacted.

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described in detail below with reference to an embodiment shown in the accompanying drawings.

As shown in FIG. 1, the fully automatic HDL-cholesterol analyzer A according to this embodiment employs a discrete single-line single-item analysis method. In this embodiment, the analysis speed is 120 tests / hour. The reaction time is set to 2 minutes for the pre-precipitation reaction and 10 minutes at the maximum for the color reaction.

Then, a fully automatic HDL- according to this embodiment.
The cholesterol analyzer includes a sample container 1 containing serum, a sample cassette 2 holding a plurality of the sample containers 1 in series, and a sample cassette transfer device for intermittently transferring the sample cassette 2 to a sampling position a (see FIG. (Not shown), a sample pipette device 4 that sucks a required amount of serum from the sample container 1 at the sampling position a and dispenses it into the reaction tube portion 3, the reaction tube portion 3 and the filtration tube storage portion 5 A plurality of filtration / reaction container bodies 6 formed therein, a cassette housing portion 7 in which a plurality of the filtration / reaction container bodies 6 are stacked in a plurality of rows, and a filtration / reaction container 7 A feeder device (not shown) that automatically feeds the container body 6 to the start end of the cassette transfer path 8 and the filtration / reaction container body 6 for sample discharge /
From the precipitating reagent discharge position b through the filter tube transfer position c to the resampling position / first reagent addition position d to second reagent addition position e, stirring position f, measurement suction position g, and container body disposal position h A container body transfer device (not shown) for sequentially transferring along the transfer path 8 and a filter tube 10 in the reaction tube part 3 from the filter tube storage position of the filtration / reaction container body 6 at the filter tube transfer position c. To the flow tube 11, an optical measuring device 12 for sucking the sample that has undergone color reaction at the measuring position g to the flow cell 11 and performing optical measurement at a predetermined wavelength, and a supernatant separated by a precipitation reagent. Resampling that sucks a required amount of sample from the inside of the filter tube 10 and discharges it to the filter tube storage unit 5.
It is composed of a first reagent pipette device 16, a second reagent device 19, a stirring device 34, and a control device CPU for organically linking and controlling the drive of these devices.

In the figure, reference numeral 20 is a power source, reference numeral 21 is a temperature control circuit, reference numeral 22 is an operation portion such as a keyboard, reference numeral 23 is a display portion constituted by a CRT and the like, reference numeral 24 is a printer, Reference numeral 25 is a precipitation reagent bottle, reference numeral 26 is a first reagent bottle, reference numeral 27 is a second reagent bottle, reference numeral 28 is a sampling / precipitation reagent pump, reference numeral 29 is a resampling / first reagent pump, reference numeral 30. Indicates a second reagent pump, reference numeral 31 indicates a wash water supply pump, reference numeral 32 indicates a measurement liquid suction pump, and reference numeral 41 indicates a waste container having a substantially concave cross section.

The sample container body transfer device employs a circulation system in which the sample cassette 2 holding the sample container 1 is sequentially transferred to the sampling position a, and the number of sample sets is 60 for general samples and for the control sample. It is configured so that 6 pieces and 6 pieces of urgent samples can be set, and urgent interruption can be made at any time. The specific structure of the sample container body transfer device is described in JP-B-2-29988.
Since it is similar to that disclosed in Japanese Patent Publication No. 4-80338 and Japanese Patent Publication No. 4-80338, detailed description thereof will be omitted here.

The sample pipette device 4 includes a pipette 13
Is configured to linearly slide along the guide rail 14, and moves from directly above the cleaning trough 15 to the sampling position a and descends to move to the sampling position a.
After sucking a required amount of the serum sample from the inside of the sample container 1 which has reached the position, the sample is raised and the sample discharging / precipitating reagent discharging position b
And the serum sample sucked into the reaction tube portion 3 at the sample discharge / precipitation reagent discharge position b is discharged together with the precipitation reagent. At this time, the inside of the pipette 14 is washed with the precipitation reagent. The suction / discharge of the serum sample and the discharge of the precipitation reagent are performed by the sampling / precipitation reagent pump 28 of the microsyringe system.

After that, the pipette 14 is transferred to a position right above the cleaning trough 15, and pure water is sprayed from the cleaning water supply unit 31 onto the outer peripheral surface of the pipette 14 for cleaning.

Since the structure of the pipette 13 and the guide rail 14 of the sample pipette device 4 is the same as that of a known sample pipette device using a timing belt or the like, detailed description thereof will be omitted here.

Further, the precipitation reagent contained in the precipitation reagent bottle 25 is made of a solution containing lintantoic acid and magnesium salt.

The filtration / reaction container body 6 includes a reaction tube portion 3 having an inner diameter of φ12 × a height of 35 mm (depth of 34 mm) and an inner diameter of φ12.
5 × 35 mm height (20 mm depth) filter tube storage 5
As shown in FIGS. 2 to 5, five pieces are integrally formed in a pair, and the bottom portion 5a of the filter tube housing portion 5 has the filter tube 10 housed therein. When the 6 are vertically stacked as shown in FIG.
The upper portion of the filter tube 10 protruding upward from the bottom is raised to a height that does not collide with the bottom portion 5a of the filter tube housing portion 5 stacked above, and below the bottom portion 5a, there is a filter tube housing space portion 6b.
Are formed. In the figure, reference numeral 3a indicates the bottom of the reaction tube portion 3, and reference numeral 6a indicates a guide groove formed at the center of both ends of the cassette upper surface 6c.

The filtration / reaction container body 6 thus constructed is accommodated in a cassette storage portion 7 for accommodating a maximum of 5 rows × 1 row and 50 filtration / reaction container bodies 6 in total. The lowermost filtration / reaction container body 6 is automatically sent to the starting end portion of the container body transfer path 8 through the feeder device in order.

Although not shown in the drawings, the feeder device has a belt mechanism for sequentially transferring the lowermost filtration / reaction container body 6 to the starting end of the container body transfer path 8 and the lowermost filtration / reaction container body 6.
A stopper mechanism for dropping the filtration / reaction container body 6 immediately above to the lowermost stage when the reaction container body 6 disappears, and a sensor mechanism for detecting the presence or absence of the filtration / reaction container body 6 at the lowermost stage,
Other than that, the configuration is the same as that of the known cassette delivery mechanism, and therefore the detailed description thereof is omitted here.

Further, in the container body transfer device, the filtration / reaction container body 6 is moved from the sample discharge / precipitation reagent discharge position b through the filter tube transfer position c to the resampling position / first reagent addition position d to second reagent. It intermittently transfers to the addition position e, the stirring position f, the measurement suction position g, and the cassette disposal position h along the container transfer path 8, and is drive-controlled by a known timing belt mechanism.

The filter tube transfer device 9 is composed of a known mini-robot hand, and the mini-robot hand is placed in the filter tube storage portion 5 of the filtration / reaction container body 6 at the filter tube transfer position c. After grasping the filter tube 10 which is inserted and held in advance, it rises and moves to a position right above the reaction tube section 3, and thereafter,
After being lowered to insert the filter tube 10 into the reaction tube portion 3, the filter tube 10 is pushed toward the bottom portion 3a of the reaction tube portion 3. As a result, the filter tube 10 is pulled out from the filter tube housing portion 5 at the filter tube transfer position c and then inserted into the reaction tube portion 3.

The filter tube 10 is, as shown in FIGS. 6 and 7,
A cylindrical barrel body 10a and a bottom portion 10b of the barrel body 10a.
And a filter housing portion 10c formed below the bottom portion 10b, and a small hole 10 formed through the bottom portion 10b.
d, the dense filter 10e and the coarse filter 10f mounted in the filter storage portion 10c, and the two closely-contacting protrusions 10g provided on the outer peripheral surface of the filter storage portion 10c at the lower portion of the cylinder body 10a. It consists of and.

When the thus constructed filter tube 10 is pushed toward the bottom portion 3a of the reaction tube portion 3, as shown in FIG. 5, ribosomes other than HDL in the serum sample to which the precipitation reagent has been added are added. The protein S ₁ aggregates and does not pass through the dense filter 10e and the coarse filter 10f, and the reaction tube portion 3
And only the supernatant sample S _{2 of} HDL-cholesterol that remains inside the dense filter 10e and the coarse filter 10f.
And is collected in the cylinder body 10a.

Resampling / first reagent pipette device 1
The reference numeral 6 indicates a suction portion of the supernatant sample S ₂ separated by the precipitation reagent from the inside of the filter tube 10 at the resampling position / first reagent addition position d, and the filter tube housing part 5 from which the filter tube 10 has been removed. The pipette 17 is configured to slide linearly, moves from directly above the cleaning trough 18 to the resampling position / first reagent addition position d, and descends to resample. After suctioning a required amount of the supernatant sample from the inside of the filtration tube 10 inserted into the reaction tube section 3 that has reached the position / first reagent addition position d, it rises and moves to just above the filtration tube storage section 5. After that, the supernatant sample that descends and is sucked into the filter tube storage unit 5 is discharged together with the first reagent. At this time, the inside of the pipette 14 is washed with the first reagent. The suction / discharge of the supernatant sample and the discharge of the first reagent are carried out by the resampling / first reagent pump 29 of the microsyringe system.

After that, the pipette 17 is transferred to a position directly above the cleaning trough 18, and pure water is sprayed from the cleaning water supply unit 31 onto the outer peripheral surface of the pipette 17 for cleaning.

The structures of the pipette 17 and the slide lifting / lowering mechanism of the resample pipette device 16 are the same as those of the known reagent dispensing pipette device, and therefore detailed description thereof will be omitted here.

The first reagent contained in the first reagent bottle 26 is an enzyme reagent, and is 3.5-dimethoxy-N-ethyl-N- (2'-hydroxy-3'-sulfoprovir) aniline sodium (DAOS). ), Ascorbate oxidase (from pumpkin), and 50 mM Good's buffer pH 6.1.

The second reagent device 19 has a second reagent dispensing position e.
The _second sample in the supernatant sample S ₂ in the filter tube storage portion 5 that has reached
The second reagent contained in the reagent bottle 27 is dispensed in a required amount through a pipette 33 and a second reagent pump 30 of a microsyringe system. The pipette 33 extends right above the second reagent dispensing position e. After moving forward to dispense the second reagent, it moves backward and stands by at a position separated from the container transfer path 8. Since the pipette 33 for discharging the second reagent is exclusively used for the second reagent, there is no concern of cleaning the inside, and only the outer peripheral surface immersed in the supernatant sample S ₂ is washed water supply unit 31. After washing with pure supplied from
It is configured to drain from a wash trough (not shown).

The stirrer 34 stirs the supernatant sample S ₂ to which the second reagent has been added, which has reached the stirring position f and which has been stored in the filter tube storage portion 5. The stirrer 34 is integrally incorporated in the second reagent device 19. Is configured.

Therefore, the stirring rod (not shown) of the stirring device 34 is immersed in the supernatant sample S ₂ in the filter tube storage portion 5 at the same timing as the forward / backward / upward / downward movement of the pipette 33, and the motor ( After the supernatant sample S ₂ is stirred by the rotation operation of (not shown), it rises and moves backward and is transferred to directly above the washing trough (not shown), and the outer peripheral surface of the stirring rod is fed from the washing water supply unit 31. Supplied pure and washed.

Since the configurations of the second reagent device 19 and the stirring device 34 are the same as those of the second reagent device and the stirring device used in the conventional automatic analyzer, detailed description thereof will be omitted here. To do.

The second reagent contained in the second reagent bottle 27 is cholesterol esterase and cholesterol oxidase (CO), peroxidase (POD), 4-
Aminoantivirin and 50 mM Good's buffer pH
It is generated by adding 6.1.

The optical measuring device 12 adopts the relative measuring method by the end point method, and is a device for sucking the sample that has undergone the color reaction at the measuring position g to the flow cell 11 and performing optical measurement at a predetermined wavelength. Suction pipette 36, the suction pipette 36 and the flow cell 11
And a light source 28, a wavelength selection filter 39, and a light receiving element 40.

The measurement liquid suction pump 32 composed of a peristaltic pump or the like is communicatively connected to the downstream side of the flow cell 11 in the line 37.
The supernatant sample S ₂ aspirated by ₂ is drained into a washing trough (not shown) after the measurement is completed, and the inside of the suction pipette 36 and the line 37 and the outer peripheral surface of the suction pipette 36 are washed with water. It is washed with pure water supplied from the supply unit 31.

The light source 28 is composed of a tungsten halogen lamp, and the measurement wavelength is two wavelengths, and the main wavelength of 600 nm and the sub wavelength of 700 nm are selected by the wavelength selection filter 39. The lamp is cooled by air cooling.

The control unit CPU is composed of, for example, a microprocessor (MPU) or the like, and is configured to drive and control the above-mentioned mechanisms in cooperation with each other and store and store the measurement data in the storage unit.

The temperature control circuit 21 heats the supernatant sample S ₂ to 37 ° C. of the reaction temperature of the first reagent and the second reagent, and its configuration is the same as that of a known one. Detailed description is omitted here.

Next, a fully automatic H constructed as described above.
The operation of the DL-cholesterol analyzer will be described.

First, a required amount of the serum sample S ₁ is contained in the sample container 1, and the sample cassette 2 is held at the sample cassette setting position.
Of course, the serum sample S ₁ contained in the sample container 1 is
Information about the sample cassette 2 is not shown.
It is configured so that it can be identified by a bar code label or the like arranged on the.

Next, when a start switch (not shown) is turned on, the sample cassette 2 is sequentially transferred to the sampling position a, and in synchronism with this, the filtration / reaction container body 6 is transferred from the cassette housing portion 7 to the container body. The reaction tube portion 3 of the filtration / reaction container body 6 is automatically sent to the starting end of the transfer path 8 and transferred to the sample discharge / precipitation reagent discharge position b.

When the reaction tube portion 3 of the filtration / reaction container body 6 is transferred to the sample discharge / precipitation reagent discharge position b in this way, the sample pipette device 4 is operated, and FIG.
As shown in (A), a required amount of the serum sample S ₁ is sucked from the sample container 1 at the sampling position a and dispensed into the reaction tube section 3.

At this time, the precipitation reagent is discharged into the reaction tube section 3 together with the serum sample S ₁ through the sample pipette device 4.

As a result, as shown in FIG. 8 (B), the serum sample reacts with the precipitation solution containing lintantoic acid and magnesium salt, and riboproteins other than HDL are selectively precipitated.

After this, the filtration / reaction container body 6 is intermittently transferred to the downstream side of the container body transfer path 8, and the reaction tube portion 3 to which the above-mentioned precipitation reagent is added is sent to the filter tube transfer position c. At the filter tube transfer position c, as shown in FIG. 8 (C), the filter tube 10 previously held in the filter tube storage section 5 is connected to the reaction tube section 3 via the filter tube transfer device 9. It is pressed in.

As a result, only HDL-cholesterol permeates the filter of the filter tube 10 and is collected in the cylinder body 10a as shown in FIG. 8 (D).

Next, the filtration / reaction container body 6 is intermittently transferred to the downstream side of the container body transfer path 8 to reach the resampling position / first reagent addition position d, and the resampling position / first reagent At the reagent addition position d, as shown in FIG. 8 (E), the supernatant sample S collected in the filter tube 10 via the pipette 17 of the resampling / first reagent pipette device 16 is collected.
₂ is dispensed into the filter tube storage portion 5 in the required amount, and as shown in FIG.
As shown in (F), the required amount of the first reagent is added.

Thereafter, the filtration / reaction container body 6 is intermittently transferred to the downstream side of the container body transfer path 8 and reaches the second reagent addition position e, and at the second reagent addition position e, as shown in FIG. As shown in G), after the required amount of the second reagent is added via the second reagent device 19, it is stirred by the stirring device 34 at the stirring position f.

Thus, the first and second reagents are the supernatant sample S ₂
The cholesterol ester in the supernatant is decomposed into free cholesterol and fatty acid by the action of cholesterol esterase.

The cholesterol thus produced is oxidized by the action of cholesterol oxidase together with the existing free cholesterol, and at the same time hydrogen peroxide is produced.

The generated hydrogen peroxide is converted into sodium aniline (DA) by the action of peroxidase (POD).
OS) and 4-aminoantivirine are quantitatively oxidatively bonded to each other to form a blue pigment.

Therefore, the supernatant sample S ₂ colored in this way
8H is introduced into the flow cell 11 of the optical measuring device 12 at the measurement suction position g, its absorbance is measured at a predetermined wavelength, and the voltage-converted measurement data is stored in the controller CPU. It is stored and stored in a unit and displayed on the display unit 23 or is printed by the printer 24.

In this way, the filtration and
The reaction container body 6 is intermittently transferred to the downstream side of the container body transfer path 8, reaches the container body disposal position h, and is discarded in the waste container 41.

[0055]

As described above, according to the fully automatic HDL-cholesterol analyzer of the present invention, the HDL
-As a cholesterol measurement method, a binding precipitation method that is useful for routine routine tests is used, and all the processes required for this binding precipitation method are automated without human intervention, and this type of test is performed continuously. It has a number of excellent effects.

[Brief description of drawings]

FIG. 1 is an explanatory diagram showing a schematic configuration of a fully automatic HDL-cholesterol analyzer according to an embodiment of the present invention.

FIG. 2 is a plan view of a filtration / reaction container body used in the fully automatic HDL-cholesterol analyzer.

FIG. 3 is a front view of the filtration / reaction container body.

FIG. 4 is a cross-sectional view of the filtration / reaction container body.

FIG. 5 is an enlarged cross-sectional view showing a state in which a filtration tube is inserted into a reaction tube portion of the filtration / reaction container body.

FIG. 6 is a cross-sectional view of the filter tube.

FIG. 7 is a bottom view of the filter tube.

FIG. 8 is a process explanatory diagram sequentially showing processing steps by the fully automatic HDL-cholesterol analyzer.

[Explanation of symbols]

 1 sample container 2 sample cassette 3 reaction pipe part 4 sample pipette device 5 filtration pipe storage part 6 filtration / reaction container body 8 container body transfer path 9 filtration pipe transfer device 10 filtration pipe 11 flow cell 12 optical measurement device 16 resampling / first 1 Reagent pipette device 19 2nd reagent device CPU control device a Sampling position b Sample discharge / precipitation reagent discharge position c Filter tube transfer position d Resampling position / First reagent addition position e Second reagent addition position f Stirring position g Optical Measurement position h Disposal position

Claims (5)

[Claims] 1. A sample pipette device for dispensing a required amount of serum into a reaction tube portion from a sample container containing serum, and a filtration comprising a plurality of the reaction tube portion and a filter tube storage portion. A reaction container body and a cassette transfer device for sequentially transferring the filtration / reaction container body from a sample discharge / precipitation reagent discharge position to a filter tube transfer position to a resampling position / reagent addition position to a measurement suction position; At the filter tube transfer position, a means for transferring the filter tube from the filter tube storage position of the filtration / reaction container body into the reaction tube portion, and a sample that has undergone color reaction at the measurement position is sucked up to the flow cell to a predetermined wavelength. A fully automatic HDL-cholesterol analyzer including an optical measurement device for optical measurement and a control device for organically linking and controlling the drive. 2. The fully automatic HDL-cholesterol according to claim 1, wherein the filtration / reaction container body is integrally formed in series with the filtration tube housing portion and the reaction tube portion in a pair. Analysis equipment. 3. The filtration tube, wherein the bottom of the filtration tube housing portion of the filtration / reaction container body projects upward from the upper surface of the cassette when the filtration / reaction container bodies are vertically stacked with the filtration tube housed therein. The fully-automatic HDL-cholesterol analyzer according to claim 2, wherein the upper part of the above is raised to a height that does not collide with the bottom part of the filter tube accommodating part stacked above. 4. At the re-sampling position, a filter tube inserted into the reaction tube section is gradually pressed by a pressurizing means to filter a sample that has undergone a precipitation reaction in the reaction tube section. Full automatic HD according to any one of claims 3 to 4.
L-cholesterol analyzer. 5. At the re-sampling position, the supernatant sample filtered by a filter tube inserted into the reaction tube section is discharged into the filter tube housing section together with the first reagent for reaction. The fully automatic HDL-cholesterol analyzer according to any one of claims 1 to 4.