JPH07151651A - Sampler and sampling method - Google Patents
Sampler and sampling methodInfo
- Publication number
- JPH07151651A JPH07151651A JP30150193A JP30150193A JPH07151651A JP H07151651 A JPH07151651 A JP H07151651A JP 30150193 A JP30150193 A JP 30150193A JP 30150193 A JP30150193 A JP 30150193A JP H07151651 A JPH07151651 A JP H07151651A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- tube
- sterilization
- sampling
- sterilizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、試料の採取装置及び採
取方法に関し、特に食品工業、化学工業、薬品工業等に
おいて生きた雑菌(以下、単に雑菌という)の混入が問
題となる製品の品質管理に有用である滅菌状態での試料
採取装置及び方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sampling device and a sampling method, and in particular, the quality of products in which contamination by live bacteria (hereinafter simply referred to as bacteria) is a problem in the food industry, chemical industry, pharmaceutical industry, etc. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device and method for sampling in a sterile state which is useful for management.
【0002】[0002]
【従来の技術】食品工業、化学工業及び薬品工業等にお
いて発酵工程を経る製品、例えばビール等は、発酵中に
発酵槽や発酵槽回りの配管類内に好気性の乳酸菌等の雑
菌が混入すると、これらの生きている雑菌が時間ととも
に発酵槽や配管類内で増殖し、雑菌及び雑菌による生産
物等によって製品の品質が低下し、最終的に製品として
出荷できない場合があった。2. Description of the Related Art Products such as beer which undergo a fermentation process in the food industry, chemical industry, pharmaceutical industry, etc., are liable to be mixed with bacteria such as aerobic lactic acid bacteria in the fermentation tank or the pipes around the fermentation tank during fermentation. In some cases, these live germs grow in the fermenter or the pipes with time, and the quality of the product is deteriorated due to the germs and products produced by the germs, and the product cannot be finally shipped as a product.
【0003】そのため、製品の品質管理のため、発酵槽
や発酵槽回りの配管類内のアルコール濃度や乳酸菌等の
量を定期的に分析する必要がある。しかしながら、試料
を採取するときに乳酸菌等の雑菌が採取試料中に混入す
ると、もともと発酵槽や配管類内に混入していた雑菌で
あるのか、試料採取時に外部から採取試料のみに混入し
た雑菌であるのかの区別が不可能となり、製品の品質管
理上不都合を生じていた。Therefore, in order to control the quality of the product, it is necessary to periodically analyze the alcohol concentration and the amount of lactic acid bacteria in the fermenter and the pipes around the fermentor. However, if miscellaneous bacteria such as lactic acid bacteria are mixed into the collected sample when collecting the sample, it may be that the bacteria were originally mixed in the fermenter or pipes, or the miscellaneous bacteria mixed only in the collected sample from the outside at the time of sampling. It became impossible to distinguish whether or not there was, which caused inconvenience in quality control of the product.
【0004】このような理由から、発酵工程を経る製品
の品質管理において試料採取時、特に発酵槽やその周囲
の配管類への雑菌の混入を防止することは極めて重要で
ある。For these reasons, it is extremely important to prevent contamination of bacteria in the fermentation tank and the pipes around it, especially when collecting samples in quality control of products that undergo fermentation processes.
【0005】従来、発酵槽からの試料採取は、発酵槽に
試料導管を設け、この試料導管とドレン管とを試料弁に
より接続し、このドレン管に水蒸気を導入し、ドレン管
自体を121℃以上に保持した状態で30分間程度水蒸
気を流通させ滅菌した後、放冷し、次いで滅菌した空気
を放冷後のドレン管に流通し、ドレン水を排出、乾燥し
てドレン管を高温滅菌した後、発酵槽から試料液をドレ
ン管を通してドレン管に接続された試料管へ移液する方
法により行われてきた。Conventionally, for sampling from a fermenter, a sample conduit is provided in the fermentor, the sample conduit and the drain pipe are connected by a sample valve, steam is introduced into the drain pipe, and the drain pipe itself is 121 ° C. After sterilizing by letting steam circulate for about 30 minutes in the above-retained state, it was left to cool, and then sterilized air was passed through the drain pipe after being left to cool, drain water was discharged and dried to sterilize the drain pipe at high temperature. After that, it has been carried out by a method of transferring a sample solution from a fermenter through a drain tube to a sample tube connected to the drain tube.
【0006】しかしながら、ある種の発酵製品において
は、水蒸気による高温滅菌は適当ではない。例えば、ビ
ール製造においては、発酵槽を0〜10℃の温度で管理
する必要があり、水蒸気で高温加熱滅菌することにより
ドレン管に接続する試料弁、試料導管、発酵槽も加熱さ
れるので、発酵槽の温度管理が困難になる。そのため、
発酵槽が高温になることが好ましくない製品については
水蒸気の替わりにエタノールによる滅菌が提案されてい
る。However, high temperature sterilization with steam is not suitable for some fermented products. For example, in beer production, it is necessary to control the fermenter at a temperature of 0 to 10 ° C., and since the sample valve connected to the drain pipe, the sample conduit, and the fermenter are also heated by high-temperature heat sterilization with steam, Temperature control of the fermenter becomes difficult. for that reason,
Sterilization with ethanol instead of steam has been proposed for products in which it is not desirable to heat the fermenter at high temperatures.
【0007】図5は、従来の試料採取方法の代表的な1
例を示すフローチャートである。図中、501は発酵
槽、502は試料導管、503は試料弁、504はドレ
ン管、505は試料管、506は採取された試料液、5
07は水蒸気導入管、508はエタノール又はエタノー
ル水溶液導入管、509は滅菌空気導入管である。しか
しながら、この方法においては、人間が試料管を交換す
る必要があるので、その交換工程が煩雑であり、また、
この煩雑さを避けるため試料管交換を自動にして完全滅
菌する為にはクリーンルーム等の大がかりな設備が必要
である、という問題がある。FIG. 5 shows a typical one of the conventional sampling methods.
It is a flowchart which shows an example. In the figure, 501 is a fermenter, 502 is a sample conduit, 503 is a sample valve, 504 is a drain pipe, 505 is a sample pipe, 506 is a sample liquid sampled, 5
Reference numeral 07 is a steam introducing pipe, 508 is an ethanol or ethanol aqueous solution introducing pipe, and 509 is a sterilizing air introducing pipe. However, in this method, it is necessary for a human to replace the sample tube, so the replacement process is complicated, and
In order to avoid this complication, there is a problem that a large-scale facility such as a clean room is required in order to automatically sterilize the sample tubes and perform complete sterilization.
【0008】一方、発酵槽に設けた試料弁を火炎滅菌し
て雑菌の混入を防いだ後、予め滅菌した試料管に試料弁
から開放系にて直接に試料を採取する方法が提案されて
いる。この方法においては、閉鎖系にみられる煩雑な試
料管交換が不要になるが、試料の採取が開放系で行われ
るために、試料の飛散や雑菌の混入を防いで試料を採取
するためには、高度に熟練した人間が必要であった。ま
た、高度に熟練した人間により試料が採取されても、試
料採取中の雑菌混入が原因とみられる製品の汚染、即
ち、製品品質の低下が1年間に複数回発生していた。On the other hand, there has been proposed a method in which a sample valve provided in a fermenter is sterilized by flame to prevent contamination of various bacteria, and then a sample is directly collected from a sample valve in an open system into a previously sterilized sample tube. . In this method, the complicated sample tube exchange found in a closed system is unnecessary, but since the sample collection is performed in an open system, it is necessary to collect the sample while preventing the sample from scattering and mixing of various bacteria. , Required highly skilled humans. Further, even when a sample is collected by a highly skilled person, contamination of the product, which is considered to be caused by contamination with other bacteria during the sample collection, that is, deterioration of product quality, occurs several times a year.
【0009】[0009]
【発明が解決しようとする課題】本発明は、上記従来技
術に鑑みて、閉鎖系における試料管交換を簡略化して閉
鎖系において完全に滅菌した状態で試料を採取する方法
を提供することを目的とする。SUMMARY OF THE INVENTION In view of the above-mentioned prior art, it is an object of the present invention to provide a method for collecting a sample in a closed system in a completely sterilized state by simplifying sample tube exchange in the closed system. And
【0010】また、本発明は、人為差のある手作業に替
えて試料の採取及び試料管の交換をクリーンルーム等の
大がかりな設備を必要とせずに自動化することを目的と
する。Another object of the present invention is to automate sample collection and sample tube replacement in place of manual work with human error without requiring large-scale equipment such as a clean room.
【0011】[0011]
【課題を解決するための手段】上記目的を達成する本発
明は、滅菌材を導入する滅菌材導入口、不要な滅菌材及
び試料を排出するドレン排出口及び試料を導入する試料
導入口を有するドレン管と、滅菌材を導入する滅菌材導
入口を有し且つ一端が開放された滅菌筒と、前記滅菌筒
内へ着脱可能で且つ予め減圧滅菌密閉された試料採取管
と、予めドレン管に装着され、試料移送時にドレン管と
滅菌筒と試料採取管とを接続してドレン管内の試料を試
料採取管に移送する中空針とを有してなる試料採取装置
である。The present invention for achieving the above object has a sterilizing material inlet for introducing a sterilizing material, a drain outlet for discharging unnecessary sterilizing material and a sample, and a sample inlet for introducing a sample. A drain pipe, a sterilizing cylinder having a sterilizing material introducing port for introducing a sterilizing material, and one end open, a sampling tube that is detachably attachable to the sterilizing cylinder and sealed under reduced pressure sterilization in advance, A sample collecting device that is mounted and has a hollow needle that connects a drain tube, a sterilizing cylinder, and a sample collecting tube to transfer the sample in the drain tube to the sample collecting tube when the sample is transferred.
【0012】また、本発明の装置は、ドレン管の一端、
滅菌筒の一端及び試料採取管の一端をシリコンゴムで構
成し、中空針を該シリコンゴム部分に貫通させてドレン
管、滅菌筒及び試料採取管を接続して試料を移送するこ
とを含むものである。Also, the device of the present invention comprises one end of the drain pipe,
One end of the sterilization cylinder and one end of the sample collection tube are made of silicone rubber, and a hollow needle is passed through the silicone rubber portion to connect the drain tube, the sterilization cylinder and the sampling tube to transfer the sample.
【0013】また、本発明は、上記試料採取装置を使用
して、滅菌材導入口よりドレン管内に滅菌材を導入し、
ドレン排出口から滅菌材を排出し、ドレン管内を洗浄滅
菌、乾燥する工程と、滅菌材導入口よりドレン管内に滅
菌材を導入し、導入された滅菌材を滅菌筒に挿入された
中空針の先端部より排出し、中空針内部を洗浄滅菌、乾
燥する工程と、滅菌筒内に試料採取管を中空針と接触し
ない位置まで挿入する工程と、滅菌材導入口より滅菌筒
内に滅菌材を導入し、滅菌筒内雰囲気並びに滅菌筒内の
中空針及び試料採取管の外部表面を洗浄滅菌、乾燥する
工程と、試料導入口よりドレン管内に試料を導入する工
程と、滅菌された気体を、滅菌筒の滅菌材導入口から滅
菌筒内へ導入しながら、中空針をその内部が予め減圧滅
菌された試料採取管に挿入することにより試料を試料採
取管内に移送する工程とを有することを特徴とする試料
採取方法である。Further, according to the present invention, the sterilizing material is introduced into the drain pipe through the sterilizing material introducing port by using the above-mentioned sampling device.
The process of discharging the sterilizing material from the drain discharge port, washing and sterilizing the inside of the drain pipe, and drying, and introducing the sterilizing material into the drain pipe through the sterilizing material introducing port, and introducing the sterilizing material into the sterilization tube. Discharging from the tip, washing and sterilizing the inside of the hollow needle, drying, inserting the sample collection tube into the sterilization cylinder to a position where it does not contact the hollow needle, and sterilizing material into the sterilization cylinder from the sterilization material inlet. Introduce the atmosphere in the sterilization cylinder and the washing needle sterilization of the hollow needle in the sterilization cylinder and the external surface of the sampling tube, the step of introducing the sample into the drain tube from the sample introduction port, and the sterilized gas, A step of transferring the sample into the sample collecting tube by inserting the hollow needle into the sample collecting tube whose inside is sterilized in advance under reduced pressure while being introduced into the sterile tube from the sterilizing material introduction port of the sterilizing cylinder. This is a sampling method.
【0014】また、本発明の方法は、滅菌材がエタノー
ル水溶液と滅菌された気体であることを含むものであ
る。Further, the method of the present invention includes that the sterilizing material is an aqueous ethanol solution and sterilized gas.
【0015】以下、本発明を図面を参照しつつ詳細に説
明する。Hereinafter, the present invention will be described in detail with reference to the drawings.
【0016】図1は、本発明の試料採取装置の1態様を
示すフロー図である。FIG. 1 is a flow chart showing one embodiment of the sampling device of the present invention.
【0017】図中、1は本発明装置により試料が採取さ
れる発酵槽等の被検槽(以下、単に発酵槽という)であ
り、通常は試料導管2が設置され、試料導管2の先端に
は試料弁3が設置されている。In the figure, reference numeral 1 is a test tank such as a fermenter in which a sample is collected by the device of the present invention (hereinafter simply referred to as a fermenter), and a sample conduit 2 is usually installed at the tip of the sample conduit 2. Is provided with a sample valve 3.
【0018】本発明において、ドレン管4、中空針5及
び滅菌筒8内部を滅菌乾燥するための滅菌材は、洗浄滅
菌作用のある滅菌液12と、滅菌液12を蒸発乾燥させ
るための気体からなることが好ましい。In the present invention, the sterilizing material for sterilizing and drying the inside of the drain pipe 4, the hollow needle 5 and the sterilizing cylinder 8 includes a sterilizing liquid 12 having a washing and sterilizing action and a gas for evaporating and drying the sterilizing liquid 12. It is preferable that
【0019】前記滅菌液12としては、(1)濃度60
%以上のエタノール水溶液、あるいは(2)濃度1pp
m以上4000ppm以下の次亜塩素酸、次亜塩素酸ナ
トリウム又は次亜塩素酸カリウムの水溶液、あるいは
(3)濃度3%以上35%以下の過酸化水素水溶液を単
独又は組み合わせて使用することができる。尚、上記
(2)や(3)の滅菌液を単独に又は組み合わせて使用
する場合には、それらの滅菌液で洗浄滅菌後、最後に少
なくとも1回は上記(1)のエタノール水溶液で洗浄滅
菌することが好ましい。The sterilizing solution 12 has (1) a concentration of 60
% Or more ethanol aqueous solution, or (2) concentration 1 pp
An aqueous solution of m or more and 4000 ppm or less of hypochlorous acid, sodium hypochlorite or potassium hypochlorite, or (3) an aqueous solution of hydrogen peroxide having a concentration of 3% or more and 35% or less can be used alone or in combination. . When the sterilizing liquids of (2) and (3) above are used alone or in combination, they are washed and sterilized with these sterilizing liquids, and finally washed and sterilized with the aqueous ethanol solution of (1) at least once. Preferably.
【0020】滅菌液12を蒸発乾燥させるための気体と
しては、滅菌フィルター21、38を通して滅菌した空
気、窒素、ヘリウム、アルゴン等が使用できる。尚、雑
菌が乳酸菌等の好気性菌である場合には滅菌した空気
を、メタン菌等の嫌気性菌である場合には窒素、ヘリウ
ム、アルゴン等を使用するのが好ましい。As the gas for evaporating and drying the sterilizing liquid 12, air sterilized through the sterilizing filters 21 and 38, nitrogen, helium, argon or the like can be used. It is preferable to use sterilized air when the bacteria are aerobic bacteria such as lactic acid bacteria and nitrogen, helium, argon or the like when the bacteria are anaerobic bacteria such as methane bacteria.
【0021】例えば、滅菌液12としてエタノール使用
液を使用する場合は、その滅菌作用は周知のようにエタ
ノールが蒸発するときに作用するものである。従って、
乾燥用気体供給器17、滅菌フィルター21、分散器2
8を介して滅菌された気体を供給することにより滅菌と
乾燥が同時に行われる。このときの、エタノール水溶液
の濃度は、乾燥を主目的とする場合には90〜100%
とすることが乾燥時間が短縮されるので好ましく、滅菌
を主目的とする場合には60〜80%、更に好ましくは
滅菌効果の顕著な65〜75%とすればよい。For example, when a solution using ethanol is used as the sterilizing solution 12, its sterilizing action works when ethanol is evaporated, as is well known. Therefore,
Drying gas supplier 17, sterilizing filter 21, disperser 2
By supplying a sterilized gas via 8, sterilization and drying are performed simultaneously. At this time, the concentration of the aqueous ethanol solution is 90 to 100% when the main purpose is drying.
The drying time is shortened, and when the main purpose is sterilization, it is 60 to 80%, and more preferably 65 to 75% where the sterilization effect is remarkable.
【0022】また、上記のように、滅菌液12として次
亜塩素酸、次亜塩素酸ナトリウム、次亜塩素酸カリウム
及び過酸化水素水溶液のうち少なくとも1種と、エタノ
ール水溶液とを併用する場合には、最終的に100%エ
タノールで洗浄し乾燥させることが、上記滅菌液の塩類
を除去する観点から好ましい。Further, as described above, in the case where at least one of hypochlorous acid, sodium hypochlorite, potassium hypochlorite and an aqueous solution of hydrogen peroxide is used as the sterilizing liquid 12 in combination with an aqueous solution of ethanol. Is preferably washed with 100% ethanol and dried from the viewpoint of removing salts from the sterilized solution.
【0023】また、滅菌された気体を供給するための圧
力は、ドレン管の大きさ、中空針の長さ及び内径に応じ
て設定することができるが、概ね水柱で10mm〜20
0mmで供給できるように調節すれば特に不都合は生じ
ない。中空針5の長さや内径によっても異なるが、水柱
で10mm未満の場合は、滅菌及び乾燥に時間がかかり
すぎることがあり、水柱で200mmを越えると必要以
上に滅菌された気体を使用することがあるためである。The pressure for supplying the sterilized gas can be set according to the size of the drain pipe, the length and the inner diameter of the hollow needle, and is generally 10 mm to 20 mm in a water column.
If it is adjusted so that it can be supplied at 0 mm, there will be no particular inconvenience. Although it depends on the length and inner diameter of the hollow needle 5, if the water column is less than 10 mm, sterilization and drying may take too long. If the water column exceeds 200 mm, it is possible to use sterilized gas more than necessary. Because there is.
【0024】上記のごとき滅菌材をドレン管4に導入す
るためにドレン管4に設ける滅菌材導入口48はポンプ
18により供給される滅菌液12の導入口と乾燥用気体
供給器17から供給される乾燥用気体の導入口とを同一
のものとしてもよいし、別個に設けても構わない。ま
た、滅菌材導入口48先端部には滅菌材を効率よくドレ
ン管内部に分散させるための分散器28を設けることが
好ましい。The sterilizing material inlet 48 provided in the drain pipe 4 for introducing the sterilizing material as described above into the drain pipe 4 is supplied from the inlet of the sterilizing liquid 12 supplied by the pump 18 and the drying gas supplier 17. The inlet for the drying gas may be the same or may be provided separately. Further, a disperser 28 for efficiently dispersing the sterilizing material inside the drain pipe is preferably provided at the tip of the sterilizing material introducing port 48.
【0025】図2は、分散器28の1例を示した模式図
である。分散器28としては、両端を閉鎖したパイプの
表面に複数の孔を開けたもの等を使用できるが、表面に
滅菌材を分散させるための孔を設けられる形状であれば
特に制限はない。孔の内径は分散器28の大きさによる
が、内径0.5〜2mm程度でよい。分散器28の材質
としては、試料や滅菌材に対し不活性な材質であれば特
に制限はなく、例えばステンレス鋼、耐圧ガラス、ポリ
テトラフルオロエチレン等を使用することができる。FIG. 2 is a schematic diagram showing an example of the disperser 28. As the disperser 28, a pipe having a plurality of holes opened on the surface of a pipe whose both ends are closed can be used, but it is not particularly limited as long as the surface is provided with holes for dispersing a sterilizing material. The inner diameter of the hole depends on the size of the disperser 28, but may be about 0.5 to 2 mm. The material of the disperser 28 is not particularly limited as long as it is a material that is inert to the sample and the sterilizing material, and, for example, stainless steel, pressure resistant glass, polytetrafluoroethylene or the like can be used.
【0026】ドレン管4の形状は、試料の滞留部分が少
なく、洗浄、滅菌、乾燥が容易な形状であればよい。図
1においては、横向き円筒管上部に直方体を接続した形
状であり、直方体が接続された側の円筒管底部を洗浄し
やすいように切り欠いた形状としている。この例におい
ては、直方体部分は滅菌材導入口48から導入された滅
菌材を分散させる分散器28等が設置され、横向き円筒
管部分は試料の滞留部分となる。尚、ドレン管4におい
て、試料が滞留する部分の容積は、採取する試料の量に
もよるが、概ね必要とする分析用試料量の2倍以上3倍
以下を選択すればよい。The drain tube 4 may have any shape as long as it has a small amount of sample retention and is easy to clean, sterilize and dry. In FIG. 1, a rectangular parallelepiped is connected to the upper portion of the horizontally oriented cylindrical tube, and the cylindrical tube bottom portion on the side to which the rectangular parallelepiped is connected is cut out for easy cleaning. In this example, the rectangular parallelepiped portion is provided with a disperser 28 or the like for dispersing the sterilizing material introduced from the sterilizing material introducing port 48, and the sideways cylindrical tube portion serves as a sample retaining portion. It should be noted that the volume of the portion of the drain pipe 4 where the sample is retained depends on the amount of the sample to be collected, but may be selected to be approximately 2 times or more and 3 times or less the required amount of the analysis sample.
【0027】ドレン管4の材質は試料及び滅菌材に対し
不活性な材質であれば、特に制限なく使用することが可
能であり、例えば、ステンレス鋼等が使用できる。As the material of the drain pipe 4, any material can be used without particular limitation as long as it is an inert material with respect to the sample and the sterilizing material. For example, stainless steel or the like can be used.
【0028】ドレン排出口43は、液が滞留するドレン
管4下部に設けることが好ましい。The drain discharge port 43 is preferably provided in the lower portion of the drain pipe 4 where the liquid stays.
【0029】試料導入口は、発酵槽1に付設された試料
弁3と接続され、その接続方法は、通常の接続手段であ
るボルトナット、サニタリー継手等を使用することがで
き、接続部分のシールはポリテトラフルオロエチレン、
シリコンゴム等のパッキンを使用すればよい。The sample introduction port is connected to the sample valve 3 attached to the fermenter 1, and the connection method can use a normal connecting means such as a bolt nut, a sanitary joint, etc. Is polytetrafluoroethylene,
A packing such as silicone rubber may be used.
【0030】滅菌筒8は、一端が開放された円筒状の形
状をなし、滅菌材をスプレーするスプレー口7が1つ以
上設けられていることが好ましい。It is preferable that the sterilization cylinder 8 has a cylindrical shape with one end open and is provided with one or more spray ports 7 for spraying a sterilizing material.
【0031】図3は、滅菌筒8を開放端からみた平面図
である。図中5は中空針、8は滅菌筒、7は滅菌筒8に
設けられたスプレー口である。FIG. 3 is a plan view of the sterilization cylinder 8 seen from the open end. In the figure, 5 is a hollow needle, 8 is a sterilization cylinder, and 7 is a spray port provided in the sterilization cylinder 8.
【0032】滅菌筒8の閉鎖された一端には中空針5が
貫通し得る栓6が設けられている。図4は、滅菌筒8の
中空針5が貫通する栓6の周囲を拡大した断面模式図で
ある。滅菌筒8と中空針5とは滅菌筒8に付属している
栓6で連結、シールされる。栓6はシリコンゴム栓等で
よく、押さえ45及びキャップ46で固定される。押さ
え及びキャップの材質は特に制限はないが、ステンレス
鋼等の金属製のもの、ポリテトラフルオロエチレン、ポ
リスチレン、ポリエチレン等の高分子物質を用いればよ
い。キャップ46と滅菌筒8との固定は、滅菌筒8及び
キャップ46にネジ切りすることで達成される。At the closed end of the sterilization cylinder 8, a plug 6 through which the hollow needle 5 can pass is provided. FIG. 4 is a schematic cross-sectional view in which the periphery of the stopper 6 through which the hollow needle 5 of the sterilization cylinder 8 penetrates is enlarged. The sterilization cylinder 8 and the hollow needle 5 are connected and sealed by a stopper 6 attached to the sterilization cylinder 8. The stopper 6 may be a silicone rubber stopper or the like, and is fixed by the presser 45 and the cap 46. The material of the retainer and the cap is not particularly limited, but a metal material such as stainless steel or a polymer material such as polytetrafluoroethylene, polystyrene, or polyethylene may be used. The fixation of the cap 46 and the sterilization cylinder 8 is achieved by threading the sterilization cylinder 8 and the cap 46.
【0033】滅菌筒8は、滅菌液12及び滅菌された気
体を滅菌筒8内に送り、滅菌筒8内部及び滅菌筒8内部
に挿入されている中空針5及び試料採取管10の外部を
滅菌及び乾燥する目的で設置される。また、一方で中空
針5の先端から排出されるドレンをスプレー口7を介し
て供給される滅菌材で洗浄する目的も兼ね備えている。The sterilization cylinder 8 sends the sterilization liquid 12 and sterilized gas into the sterilization cylinder 8 to sterilize the inside of the sterilization cylinder 8 and the outside of the hollow needle 5 and the sampling tube 10 inserted into the sterilization cylinder 8. And it is installed for the purpose of drying. On the other hand, it also has the purpose of cleaning the drain discharged from the tip of the hollow needle 5 with a sterilizing material supplied through the spray port 7.
【0034】滅菌筒8の材質は、分析用試料13及び滅
菌材に対して安定な材質のものであれば問題なく使用さ
れる。また、目視で分析用試料13を観察する場合に
は、硬質ガラス、石英ガラス等の透明なガラス類、ポリ
エステル、ポリエチレン等の透明な高分子製品を用いる
とよい。The material of the sterilization cylinder 8 can be used without any problem as long as it is stable with respect to the analysis sample 13 and the sterilization material. Further, when visually observing the analytical sample 13, it is preferable to use transparent glasses such as hard glass and quartz glass, and transparent polymer products such as polyester and polyethylene.
【0035】試料採取管10は、ポリエステル、硬質ガ
ラス製等の予め減圧及び滅菌された市販の採血管等を使
用することができる。市販品以外のものを用いる場合に
は、減圧滅菌密閉方法としては、以下の方法がとられ
る。 (1)予め用意したポリエステル、硬質ガラス製等の円
筒管にゴム製の栓をし、 (2)栓には栓を貫いてガラス製の出入口を設けるか、
別途用意されたその先端が封じられ横に穴を有する中空
針で出入口を設ける。 (3)設けられた出入口から滅菌効果の著しい65〜7
5%のエタノール水溶液で満たした後、予め滅菌された
乾燥用気体を流し、内部を滅菌乾燥する。 (4)滅菌乾燥後、予め滅菌された乾燥用気体を流し調
節しながら別途用意した真空ポンプで200mmHg〜
300mmHg程度に減圧する。 (5)栓を貫いてガラス製の出入口を設けた場合には、
出入口を焼き切って封じればよく、一方、先端が封じら
れ横に孔を有する中空針で出入口を設けた場合には、中
空針を抜けばよい。As the sample collecting tube 10, a commercially available blood collecting tube made of polyester, hard glass or the like which has been decompressed and sterilized in advance can be used. When a non-commercially available product is used, the following method can be used as the vacuum sterilization and sealing method. (1) A rubber stopper is attached to a cylindrical tube made of polyester, hard glass or the like prepared in advance, and (2) a glass inlet / outlet is provided through the stopper, or
The entrance and exit is provided by a separately prepared hollow needle whose tip is sealed and has a hole in the side. (3) 65 to 7 where the sterilization effect is remarkable from the entrance provided
After filling with a 5% ethanol aqueous solution, a pre-sterilized gas for drying is flowed to sterilize and dry the inside. (4) After sterilization and drying, 200 mmHg-
The pressure is reduced to about 300 mmHg. (5) If a glass door is provided through the stopper,
The inlet / outlet may be burnt out and sealed. On the other hand, if the inlet / outlet is provided by a hollow needle having a sealed tip and a lateral hole, the hollow needle may be removed.
【0036】栓9としては中空針5が貫通できるように
シリコンゴム、ブチルゴム製のものが好ましい。The stopper 9 is preferably made of silicone rubber or butyl rubber so that the hollow needle 5 can penetrate therethrough.
【0037】試料採取管10の直径及び長軸方向の長さ
は、採取すべき分析用試料13の量によって規定される
が、通常の試料採取の場合は市販の採血管において通常
に使用される大きさのものでよい。尚、試料採取管10
の長軸方向の長さは少なくとも滅菌筒8の長軸方向の長
さよりも長い方が操作性の面で好ましい。The diameter of the sampling tube 10 and the length in the long axis direction are defined by the amount of the analysis sample 13 to be collected, but in the case of normal sampling, it is usually used in a commercially available blood collection tube. Any size is acceptable. The sampling tube 10
The length in the long axis direction of is preferably at least longer than the length of the sterilization cylinder 8 in the long axis direction in terms of operability.
【0038】滅菌筒8及び試料採取管10は試料採取管
10の着脱のしやすさを考慮して、円筒状とすることが
好ましい。滅菌筒8内に試料採取管10が挿入される際
の両者の間の隙間は1〜4mmの範囲でどの位置におい
ても等しく設定される。このとき必要に応じて試料採取
管10を正しい位置に挿入するためのガイド板等を設け
てもよい。滅菌筒8と試料採取管10との隙間を均一に
することにより、滅菌液の蒸気と滅菌気体との混合流体
もしくは滅菌気体が均一に滅菌筒8の系外に放出され
る。尚、前記隙間が1mm未満の時は、試料採取管10
を滅菌筒8に挿入したり、滅菌筒8から引き抜いたりす
る場合の操作性が悪くなり、前記隙間が4mmを越える
と系外に排出される流体の均一性が悪くなるものであ
る。The sterilizing cylinder 8 and the sample collecting tube 10 are preferably cylindrical in consideration of the ease of attachment and detachment of the sample collecting tube 10. When inserting the sample collection tube 10 into the sterilization cylinder 8, the gap between them is set to be equal at any position within a range of 1 to 4 mm. At this time, a guide plate or the like for inserting the sample collection tube 10 into a correct position may be provided as needed. By making the gap between the sterilization cylinder 8 and the sample collection tube 10 uniform, the mixed fluid of the sterilization liquid vapor and the sterilization gas or the sterilization gas is uniformly discharged out of the system of the sterilization cylinder 8. When the gap is less than 1 mm, the sampling tube 10
When inserting into the sterilization cylinder 8 or withdrawing it from the sterilization cylinder 8, the operability deteriorates, and when the gap exceeds 4 mm, the uniformity of the fluid discharged to the outside of the system deteriorates.
【0039】分析用試料13をドレン管4から試料採取
管10に移送するための中空針5は、ドレン管4に中空
針接続口を設け接着又は固着してもよいが、洗浄、交換
等の便のため図1に示すようにゴム栓14等を貫通さ
せ、更にゴム栓14がドレン管4から外れないよう、押
え15を介しキャップ16をかぶせてドレン管4に接続
することが好ましい。押え15、キャップ16の材質
は、特に制限はないが、ステンレス鋼等の金属製のも
の、ポリテトラフルオロエチレン、ポリスチレン、ポリ
エチレン等の高分子物質等が好ましい。また、押え15
には中空針5を開閉するための弁機能を設けることが好
ましい。The hollow needle 5 for transferring the sample 13 for analysis from the drain pipe 4 to the sampling pipe 10 may be attached or fixed by providing a hollow needle connection port in the drain pipe 4, but it may be washed or replaced. For convenience, it is preferable to penetrate the rubber plug 14 or the like as shown in FIG. 1 and to connect the rubber plug 14 to the drain pipe 4 with a cap 16 over the presser 15 so that the rubber plug 14 does not come off from the drain pipe 4. The material of the presser foot 15 and the cap 16 is not particularly limited, but a metal material such as stainless steel or a polymer material such as polytetrafluoroethylene, polystyrene or polyethylene is preferable. Also, presser foot 15
Is preferably provided with a valve function for opening and closing the hollow needle 5.
【0040】中空針5の材質は、ステンレス鋼、鉄、ア
ルミニウム等の金属である。The material of the hollow needle 5 is a metal such as stainless steel, iron or aluminum.
【0041】次に、上記試料採取装置を用いて試料採取
する方法について説明する。Next, a method of sampling with the above-described sampling device will be described.
【0042】通常、分析のための試料を採取するとき
は、少なくとも試料導管2に滞留している試料をドレン
として数回繰り返し排出するが、これは本発明において
も同様である。Usually, when collecting a sample for analysis, at least the sample staying in the sample conduit 2 is repeatedly discharged several times as a drain, but this is also the case in the present invention.
【0043】まず、ドレン管4を試料弁3と前記方法に
より接続し、押え15の弁を開とし、弁44を1部開と
し、試料弁3を開とすれば試料導管2に滞留している試
料はドレン管4に導入される。試料導管2に滞留してい
る試料がすべてドレン管内に移った時点で弁44を閉じ
ればそれ以上の試料はドレン管4内には移らない。次い
で試料弁3を閉とし、弁44を開とし、滅菌された乾燥
用気体を乾燥用気体供給器17、滅菌フィルター21及
び分散器28を通して供給することによりドレン管4内
の試料は排出される。このとき、押え15の弁は閉の状
態である。押え15に弁機能を有さない場合には、中空
針5の先端部を試料採取管10の栓9の途中にとどめて
もよいし、別途用意した小型のシリコンゴム栓等を予め
滅菌して中空針5の先端にかぶせシールすることもでき
る。First, the drain pipe 4 is connected to the sample valve 3 by the above method, the valve of the presser foot 15 is opened, the valve 44 is partially opened, and when the sample valve 3 is opened, it stays in the sample conduit 2. The existing sample is introduced into the drain pipe 4. If the valve 44 is closed at the time when all the sample staying in the sample conduit 2 is transferred into the drain pipe, no more sample is transferred into the drain pipe 4. Then, the sample valve 3 is closed, the valve 44 is opened, and the sterilized drying gas is supplied through the drying gas supplier 17, the sterilizing filter 21 and the disperser 28, and the sample in the drain pipe 4 is discharged. . At this time, the valve of the presser foot 15 is in a closed state. When the presser 15 does not have a valve function, the tip of the hollow needle 5 may be left in the middle of the stopper 9 of the sampling tube 10, or a small silicone rubber stopper or the like prepared separately may be sterilized in advance. It is also possible to cover the tip of the hollow needle 5 and seal it.
【0044】乾燥用気体供給器17としては、特に制限
はなくボンベ又はコンプレッサー等を使用することがで
き、滅菌フィルター21、38としては特に制限はなく
市販品を使用することができる。The drying gas supplier 17 is not particularly limited and can be a cylinder or a compressor, and the sterilizing filters 21 and 38 are not particularly limited and can be commercially available products.
【0045】試料導管2に滞留していた試料をドレンと
して排出した後は、ドレン管4内及び中空針内を洗浄滅
菌及び乾燥する。After the sample accumulated in the sample conduit 2 is discharged as a drain, the inside of the drain pipe 4 and the inside of the hollow needle are washed and sterilized and dried.
【0046】まず、滅菌液タンク11内の滅菌液12を
ポンプ18、分散器28を介してドレン管4に供給し、
弁44を介して排出する。ライン47から排出されるド
レンが無色透明になったら弁44を閉じ、押さえ15の
弁を開の状態にして中空針5の先端から滅菌液を排出さ
せる。以上の操作を数回繰り返した後、改めて同一手順
にてドレン管4内に分析用試料13を受け入れる。First, the sterilizing solution 12 in the sterilizing solution tank 11 is supplied to the drain pipe 4 via the pump 18 and the disperser 28,
Drain through valve 44. When the drain discharged from the line 47 becomes colorless and transparent, the valve 44 is closed and the valve of the presser 15 is opened to discharge the sterilizing liquid from the tip of the hollow needle 5. After repeating the above operation several times, the sample 13 for analysis is again received in the drain tube 4 by the same procedure.
【0047】次いで、スプレー口7より滅菌材を導入し
て滅菌筒内を洗浄滅菌及び乾燥する。Then, a sterilizing material is introduced from the spray port 7 to wash and sterilize the inside of the sterilizing cylinder and dry it.
【0048】滅菌条件は、ドレン管4内の洗浄、中空針
5内部の洗浄、滅菌筒8内雰囲気並びに滅菌筒8内の中
空針5及び試料採取管10の外部を洗浄のいずれにおい
ても共通である。The sterilization conditions are common to the inside of the drain pipe 4, the inside of the hollow needle 5, the atmosphere inside the sterilization cylinder 8 and the outside of the hollow needle 5 and the sampling pipe 10 inside the sterilization cylinder 8. is there.
【0049】装置各部を洗浄滅菌及び乾燥した後、滅菌
筒8内に滅菌気体を流通させながら試料採取管10を滅
菌筒8内へ更に挿入し、中空針5を栓9に貫通させる。
試料採取管10内は予め減圧してあるので、試料採取管
10内に中空針5が挿入されるとドレン管4内の分析用
試料13は試料採取管10内へ移送される。移送後、中
空針5中に残留している可能性のある試料を試料採取管
10に送り出し、また、試料採取管10内の圧力を常圧
に戻す目的で、ドレン管4側からも滅菌気体を試料採取
管10へ送り込む。その後、試料採取管10を引き抜
く。After washing and sterilizing each part of the apparatus, the sampling tube 10 is further inserted into the sterilization cylinder 8 while sterilizing gas is passed through the sterilization cylinder 8, and the hollow needle 5 is penetrated into the stopper 9.
Since the inside of the sample collecting tube 10 has been depressurized in advance, when the hollow needle 5 is inserted into the sample collecting tube 10, the analysis sample 13 in the drain tube 4 is transferred into the sample collecting tube 10. After the transfer, a sample that may remain in the hollow needle 5 is sent to the sampling tube 10 and the pressure inside the sampling tube 10 is returned to normal pressure. Is sent to the sampling tube 10. Then, the sampling tube 10 is pulled out.
【0050】本発明においては、滅菌雰囲気において試
料採取管10を中空針5から引き抜くので、試料採取管
の栓9を通しての大気中の生きた乳酸菌等の雑菌の混入
を防止することができる。In the present invention, since the sample-collecting tube 10 is pulled out from the hollow needle 5 in a sterilized atmosphere, it is possible to prevent contamination of live bacteria such as lactic acid bacteria in the air through the stopper 9 of the sample-collecting tube.
【0051】以上の説明においては、手分析の場合の手
順について説明してきたが、シークエンスを組んで半自
動を含めた自動化は従来技術の組み合せで容易に行い得
る。In the above description, the procedure in the case of manual analysis has been described, but automation including semi-automation by forming a sequence can be easily performed by a combination of conventional techniques.
【0052】[0052]
【実施例】以下、実施例により本発明をより具体的に説
明するが、これらの具体例は本発明を限定するものでは
ない。The present invention will be described in more detail with reference to the following examples, but these specific examples do not limit the present invention.
【0053】実施例1 図1に示す装置を用いて容積50klのビールの発酵槽
1から以下の手順にて分析用試料13を50ml採取し
た。Example 1 Using the apparatus shown in FIG. 1, 50 ml of an analytical sample 13 was sampled from the beer fermenter 1 having a volume of 50 kl by the following procedure.
【0054】試料採取装置の概要:容積500mlのス
テンレス製のドレン管4を用意した。ドレン管4の液滞
留部分の形状は円筒形であり、その部分の容積を120
mlとした。中空針5は内径1mm、軸方向の長さ50
mmとした。押え15はポリテトラフルオロエチレン製
で、弁機能をもたせた。分散器28は内径10mm、長
さ50mmのステンレス製パイプの両端を閉鎖した形状
で直径2mmの孔を円筒側面に総計100個設けた。Outline of sampling device: A stainless drain tube 4 having a volume of 500 ml was prepared. The shape of the liquid retention portion of the drain pipe 4 is cylindrical, and the volume of that portion is 120
ml. The hollow needle 5 has an inner diameter of 1 mm and an axial length of 50.
mm. The presser foot 15 is made of polytetrafluoroethylene and has a valve function. The disperser 28 had a shape in which both ends of a stainless steel pipe having an inner diameter of 10 mm and a length of 50 mm were closed, and a total of 100 holes having a diameter of 2 mm were provided on the cylindrical side surface.
【0055】試料採取管10としては内径30mm、外
径33mm、全容積100mlの市販品を使用した。As the sample collecting tube 10, a commercially available product having an inner diameter of 30 mm, an outer diameter of 33 mm and a total volume of 100 ml was used.
【0056】滅菌筒8は内径35mm、長軸方向の長さ
100mmの円筒の底面の一方を開放し、閉鎖した他端
には、直径6mmの孔をあけ、その孔に図4に示すごと
くシリコンゴム栓6を装着し、押え45及びキャップ4
6で固定した。The sterilizing cylinder 8 has a cylindrical bottom having an inner diameter of 35 mm and a length of 100 mm in the long axis direction, one of the bottom surfaces of which is open, and the other end which is closed has a hole of 6 mm in diameter, and the hole is made of silicon as shown in FIG. Attach the rubber stopper 6, presser foot 45 and cap 4
Fixed at 6.
【0057】試料採取の準備:上記採取装置を発酵槽1
の試料弁3に装着した。パッキンはシリコンゴムを使用
した。Preparation for sample collection: Fermenter 1 with the above sampling device
The sample valve 3 was attached. Silicon rubber was used for the packing.
【0058】まず、ドレン管内部、滅菌筒内部、試料採
取管外部及び中空針内外部の洗浄、滅菌及び乾燥を以下
のように実施した。First, the inside of the drain pipe, the inside of the sterilization cylinder, the outside of the sampling pipe, and the inside and outside of the hollow needle were washed, sterilized and dried as follows.
【0059】ドレン管内部及び中空針内部の洗浄は、7
0%エタノール水溶液12を貯蔵したエタノールタンク
11から150mlの70%エタノール水溶液をポンプ
18にてライン35、33、32、31、29、25、
27及び分散器28を通しドレン管4に注入することに
より行った。押え15の弁は開閉を数回繰り返し、中空
針5の先端から70%エタノール水溶液が流出している
のを確認し、閉の状態に保持し弁44を開き70%エタ
ノール水溶液をドレン管4から放出した。For cleaning the inside of the drain pipe and the inside of the hollow needle,
From the ethanol tank 11 storing the 0% ethanol aqueous solution 12, 150 ml of 70% ethanol aqueous solution is pumped by the lines 35, 33, 32, 31, 29, 25,
It carried out by injecting into the drain pipe 4 through 27 and the disperser 28. The valve of the presser foot 15 is repeatedly opened and closed several times, and it is confirmed that the 70% ethanol aqueous solution is flowing out from the tip of the hollow needle 5, and the valve 44 is opened by keeping the valve closed and the 70% ethanol aqueous solution is discharged from the drain pipe 4. Released.
【0060】一方、滅菌筒内部、試料採取管外部及び中
空針外部の滅菌は50mlの70%エタノール水溶液1
2をポンプ18にてライン35、33、42を介して、
スプレー口7から滅菌筒8内に噴射させた。On the other hand, for sterilization inside the sterilization cylinder, outside the sampling tube and outside the hollow needle, 50 ml of 70% ethanol aqueous solution 1
2 by the pump 18 through lines 35, 33, 42,
It was sprayed from the spray port 7 into the sterile tube 8.
【0061】上記のドレン管4内部の洗浄と滅菌筒8内
の洗浄は同時に行った。次いで、乾燥用気体供給器17
から、滅菌フィルター21、38を通してそれぞれ10
00ml/分の速度で滅菌された空気をドレン管4及び
滅菌筒8に供給し滅菌及び乾燥を実施した。The inside of the drain pipe 4 and the inside of the sterilization cylinder 8 were simultaneously cleaned. Then, a drying gas supplier 17
Through sterilizing filters 21 and 38 for 10
Air sterilized at a rate of 00 ml / min was supplied to the drain pipe 4 and the sterilization cylinder 8 to carry out sterilization and drying.
【0062】ドレン管4への滅菌空気の供給を5分間継
続した後停止し、ドレン管4への試料の移液を開始し
た。まず、試料弁3を開にし、遅れてまたは同時に弁4
4を開にし、予め内容積を測定してある試料導管2の内
容積の約3倍量の試料をドレンとして抜き出しドレン排
出口43より排出した。The supply of sterile air to the drain pipe 4 was continued for 5 minutes and then stopped, and the transfer of the sample to the drain pipe 4 was started. First, the sample valve 3 is opened and the valve 4 is delayed or at the same time.
4 was opened, and a sample of about 3 times the internal volume of the sample conduit 2 whose internal volume had been measured beforehand was taken out as a drain and discharged from the drain discharge port 43.
【0063】次いで、分析用試料13を受け入れるた
め、前記手順にしたがいドレン管4内部の洗浄、滅菌及
び乾燥を行った。洗浄は3回行った後、滅菌及び乾燥を
行った。Then, in order to receive the sample for analysis 13, the inside of the drain tube 4 was washed, sterilized and dried according to the above procedure. After washing three times, sterilization and drying were performed.
【0064】分析用試料13をドレン管4に100ml
受け入れ、中空針5をシリコンゴム栓9を貫通させ、押
え15の弁を開にし試料採取管10内に分析用試料13
を50ml受け入れた。高速液体クロマトグラフィーに
より、得られた分析用試料のエタノール濃度及び糖濃度
を測定した。分析用試料45mlを目の粗さが0.2μ
mの予め滅菌されニトロセルロース製の濾紙で濾過し、
この濾紙を乳酸菌用寒天培地で72時間培養し、生きた
乳酸菌数を測定した。分析結果を表1に示す。100 ml of the sample 13 for analysis is placed in the drain tube 4.
The hollow needle 5 is received, the silicone rubber stopper 9 is penetrated, the valve of the presser 15 is opened, and the sample 13 for analysis is placed in the sampling tube 10.
Received 50 ml. The ethanol concentration and sugar concentration of the obtained analytical sample were measured by high performance liquid chromatography. 45 ml of analytical sample has a grain size of 0.2μ
m pre-sterilized nitrocellulose filter paper,
This filter paper was cultured in an agar medium for lactic acid bacteria for 72 hours, and the number of living lactic acid bacteria was measured. The analysis results are shown in Table 1.
【0065】実施例2 実施例1の容積50klのビール発酵槽1を50lの滅
菌した肉汁培地を入れた発酵槽に替え、実施例1と同様
な操作を行い、分析用試料を50ml採取した。得られ
た50mlの分析用試料を37℃の恒温槽内で48時間
保持した後、雑菌汚染の有無を調べた。結果を表2に示
す。Example 2 The beer fermenter 1 of Example 1 having a volume of 50 kl was replaced with a fermenter containing 50 liters of sterilized broth medium, and the same operation as in Example 1 was carried out to obtain 50 ml of a sample for analysis. After holding the obtained 50 ml sample for analysis in a constant temperature bath at 37 ° C. for 48 hours, the presence or absence of contamination by various bacteria was examined. The results are shown in Table 2.
【0066】参考例1 実施例1の発酵槽1を用い、図5に示す従来技術による
方法で試料液を採取した。尚、滅菌材としては121℃
の水蒸気を用いた。試料採取管の内外部は予め滅菌しク
リーンルームに保存しておいたものを用いた。採取した
分析用試料を実施例1と同様に分析した。結果を表1に
併記する。Reference Example 1 Using the fermenter 1 of Example 1, a sample solution was sampled by the conventional method shown in FIG. As a sterilizing material, 121 ° C
Of water vapor was used. The inside and outside of the sample collection tube were sterilized and stored in a clean room in advance. The collected analysis sample was analyzed in the same manner as in Example 1. The results are also shown in Table 1.
【0067】参考例2 実施例2の発酵槽1を用い、参考例1と同様に分析用試
料を採取し、実施例2と同様に検査した。結果を表2に
併記する。Reference Example 2 Using the fermenter 1 of Example 2, a sample for analysis was sampled in the same manner as in Reference Example 1 and examined in the same manner as in Example 2. The results are also shown in Table 2.
【0068】参考例3 開放系において、実施例1の発酵槽1の試料弁3から直
接に試料採取管に試料を採取した。試料採取管の内外部
は予め滅菌しクリーンルームに保存しておいたものを用
いた。採取した分析用試料を実施例1と同様に分析し
た。結果を表1に併記する。Reference Example 3 In an open system, a sample was sampled directly from the sample valve 3 of the fermenter 1 of Example 1 into a sample collection tube. The inside and outside of the sample collection tube were sterilized and stored in a clean room in advance. The collected analysis sample was analyzed in the same manner as in Example 1. The results are also shown in Table 1.
【0069】参考例4 開放系において、実施例2の発酵槽の試料弁から直接に
試料採取管に試料を採取した。試料採取管の内外部は予
め滅菌しクリーンルームに保存しておいたものを用い
た。採取した分析用試料を実施例2と同様に検査した。
結果を表2に併記する。Reference Example 4 In an open system, a sample was sampled directly from the sample valve of the fermentor of Example 2 into a sample collection tube. The inside and outside of the sample collection tube were sterilized and stored in a clean room in advance. The collected analytical sample was examined in the same manner as in Example 2.
The results are also shown in Table 2.
【0070】[0070]
【表1】 注)*1 … 検査した全サンプル数(分母)に対する乳酸
菌の発生が確認されたサンプル数(分子)で表記した。[Table 1] Note) * 1… The number of samples (numerator) in which the occurrence of lactic acid bacteria was confirmed with respect to the total number of samples (denominator) examined.
【0071】[0071]
【表2】 注)*2 … 検査した全サンプル数(分母)に対する雑菌
汚染が確認されたサンプル数(分母)で表記した。[Table 2] Note) * 2… The number of samples (denominator) confirmed to be contaminated with various bacteria against the total number of samples (denominator) tested.
【0072】[0072]
【発明の効果】本発明の試料採取装置及び試料採取方法
によれば、閉鎖系における試料採取管交換を簡略化して
閉鎖系において完全に滅菌した状態で試料を採取するこ
とが可能となる。According to the sampling device and the sampling method of the present invention, it is possible to collect a sample in a completely sterilized state in a closed system by simplifying the exchange of the sampling tube in the closed system.
【0073】また、人為差のある手作業に替えて分析用
試料の採取及び試料採取管の交換をクリーンルーム等の
大がかりな設備を必要とせずに自動化することが可能と
なる。Further, it becomes possible to automate the collection of the sample for analysis and the exchange of the sample collection tube in place of manual work with human error without requiring a large facility such as a clean room.
【図1】本発明の試料採取装置の1態様例を示す模式図
である。FIG. 1 is a schematic view showing an example of one embodiment of a sampling device of the present invention.
【図2】ドレン管に設けられる分散器の1態様例を示す
模式図である。FIG. 2 is a schematic view showing an example of one mode of a disperser provided in a drain pipe.
【図3】図1に示す滅菌筒をその開放部側からみた平面
図である。FIG. 3 is a plan view of the sterilization cylinder shown in FIG. 1 as viewed from the open side thereof.
【図4】図1に示す滅菌筒のシリコンゴム栓部分を拡大
した模式図である。FIG. 4 is an enlarged schematic view of a silicone rubber stopper portion of the sterilization cylinder shown in FIG.
【図5】従来の試料採取装置の1例を示す模式図であ
る。FIG. 5 is a schematic view showing an example of a conventional sampling device.
1 発酵槽 2 試料導管 3 試料弁 4 ドレン管 5 中空針 6、9 栓 7 スプレー口 8 滅菌筒 10 試料採取管 11 滅菌液タンク 12 滅菌液 13 分析用試料 14 ゴム栓 15、45 押え 16、46 キャップ 17 乾燥用気体供給器 21、38 滅菌フィルター 28 分散器 43 ドレン排出口 48 滅菌材導入口 501 被検槽 503 試料弁 504 ドレン管 505 試料採取管 506 試料液 507 水蒸気導入管 508 エタノール水溶液導入管 509 滅菌空気導入管 1 Fermenter 2 Sample conduit 3 Sample valve 4 Drain pipe 5 Hollow needle 6, 9 Stopper 7 Spray port 8 Sterilization cylinder 10 Sample collection pipe 11 Sterilization liquid tank 12 Sterile liquid 13 Analytical sample 14 Rubber stopper 15, 45 Presser 16, 46 Cap 17 Drying gas supplier 21, 38 Sterile filter 28 Disperser 43 Drain outlet 48 Sterile material inlet 501 Test tank 503 Sample valve 504 Drain pipe 505 Sample sampling pipe 506 Sample liquid 507 Steam inlet pipe 508 Ethanol aqueous solution inlet pipe 509 Sterile air introduction tube
Claims (4)
滅菌材及び試料を排出するドレン排出口及び試料を導入
する試料導入口を有するドレン管と、滅菌材を導入する
滅菌材導入口を有し且つ一端が開放された滅菌筒と、前
記滅菌筒内へ着脱可能で且つ予め減圧滅菌密閉された試
料採取管と、予めドレン管に装着され、試料移送時にド
レン管と滅菌筒と試料採取管とを接続してドレン管内の
試料を試料採取管に移送する中空針とを有してなる試料
採取装置。1. A sterilizing material introducing port for introducing a sterilizing material, a drain discharging port for discharging unnecessary sterilizing material and a sample, and a sample introducing port for introducing a sample, and a sterilizing material introducing port for introducing a sterilizing material. A sterilization tube having one end opened, a sampling tube that is detachably attachable to the sterilization tube, and is sealed in advance under reduced pressure sterilization, and is attached to a drain tube in advance. The drain tube, the sterilization tube, and the sample when transferring the sample A sampling device having a hollow needle that is connected to the sampling pipe and transfers the sample in the drain pipe to the sampling pipe.
採取管の一端をシリコンゴムで構成し、中空針を該シリ
コンゴム部分に貫通させてドレン管、滅菌筒及び試料採
取管を接続して試料を移送する請求項1に記載の試料採
取装置。2. One end of the drain pipe, one end of the sterilization cylinder and one end of the sampling pipe are made of silicone rubber, and a hollow needle is penetrated through the silicone rubber portion to connect the drain pipe, the sterilization pipe and the sampling pipe. The sample collecting device according to claim 1, wherein the sample is transferred by means of a method.
て、滅菌材導入口よりドレン管内に滅菌材を導入し、ド
レン排出口から滅菌材を排出し、ドレン管内を洗浄滅
菌、乾燥する工程と、 滅菌材導入口よりドレン管内に滅菌材を導入し、導入さ
れた滅菌材を滅菌筒に挿入された中空針の先端部より排
出し、中空針内部を洗浄滅菌、乾燥する工程と、 滅菌筒内に試料採取管を中空針と接触しない位置まで挿
入する工程と、 滅菌材導入口より滅菌筒内に滅菌材を導入し、滅菌筒内
並びに滅菌筒内の中空針及び試料採取管の外部表面を洗
浄滅菌、乾燥する工程と、 試料導入口よりドレン管内に試料を導入する工程と、 滅菌された気体を滅菌筒の滅菌材導入口から滅菌筒内へ
導入しながら、中空針をその内部が予め減圧滅菌された
試料採取管に挿入することにより試料を試料採取管内に
移送する工程とを有することを特徴とする試料採取方
法。3. A sterilizing material is introduced from the sterilizing material introducing port into the drain pipe by using the sampling device according to claim 1, the sterilizing material is discharged from the drain outlet, and the inside of the drain pipe is washed and sterilized and dried. And the step of introducing the sterilizing material into the drain pipe through the sterilizing material introducing port, discharging the introduced sterilizing material from the tip of the hollow needle inserted in the sterilization cylinder, washing and sterilizing the inside of the hollow needle, and drying. The step of inserting the sampling tube into the sterilization cylinder to the position where it does not come into contact with the hollow needle, and the sterilizing material is introduced into the sterilization cylinder through the sterilization material inlet, and the hollow needle inside the sterilization cylinder and the sampling needle Washing and sterilizing the outer surface of the, and drying the sample, introducing the sample into the drain tube from the sample inlet, while introducing the sterilized gas from the sterilizing material inlet of the sterilization tube into the sterilization tube, Insert inside the sample collection tube that has been sterilized under reduced pressure in advance Sampling method characterized by a step of transferring the sample into the sampling tube by Rukoto.
気体である請求項3に記載の試料採取方法。4. The sampling method according to claim 3, wherein the sterilizing material is an aqueous ethanol solution and a sterilized gas.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP30150193A JPH07151651A (en) | 1993-12-01 | 1993-12-01 | Sampler and sampling method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP30150193A JPH07151651A (en) | 1993-12-01 | 1993-12-01 | Sampler and sampling method |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH07151651A true JPH07151651A (en) | 1995-06-16 |
Family
ID=17897680
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP30150193A Pending JPH07151651A (en) | 1993-12-01 | 1993-12-01 | Sampler and sampling method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH07151651A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006502394A (en) * | 2002-10-02 | 2006-01-19 | マックス−プランク−ゲゼルシャフト ツール フェルデルング デア ヴィッセンシャフテン エー. ファオ. | Apparatus and method for liquid sample extraction |
CN109738233A (en) * | 2019-02-15 | 2019-05-10 | 中南大学湘雅医院 | A kind of low temperature oxygen-free excrement collector |
JP2021043125A (en) * | 2019-09-13 | 2021-03-18 | 株式会社テクロム | Method and device for cleaning sampling bags and liquid used therefor |
-
1993
- 1993-12-01 JP JP30150193A patent/JPH07151651A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006502394A (en) * | 2002-10-02 | 2006-01-19 | マックス−プランク−ゲゼルシャフト ツール フェルデルング デア ヴィッセンシャフテン エー. ファオ. | Apparatus and method for liquid sample extraction |
CN109738233A (en) * | 2019-02-15 | 2019-05-10 | 中南大学湘雅医院 | A kind of low temperature oxygen-free excrement collector |
JP2021043125A (en) * | 2019-09-13 | 2021-03-18 | 株式会社テクロム | Method and device for cleaning sampling bags and liquid used therefor |
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