JPH07132080A - Cell composition - Google Patents

Abstract

PURPOSE:To obtain a cell composition capable of cleaning water and maintaining qualities of water, being taken by fishes, partially supplying water- soluble vitamins and useful amino acids to fish bodies, discharging the residue, comprising a constituent material composed of an active photosynthetic bacterium and a feed for fishes as a nutrient component. CONSTITUTION:This cell composition is obtained by mixing (A) a photosynthetic bacterium belonging to the family Athiorhodaceae, Thiorhodaceae or Chloro- bacteriaceae, etc., with (B) a feed for fishes as a nutrient component and granulating. The particle diameters are preferably about 50mu to about 3.2mm. A cell discharged in a retained state of activity from fish bodies suppresses inclination of eutrophication of a closed water system in which fishes are laid and is supplied to organisms as a supply source of vitamin B group and C groups and useful amino acids.

Description

Detailed Description of the Invention

[0001]

INDUSTRIAL APPLICABILITY The present invention is useful for application to a fungal composition suitable as a feed for animals and plants, particularly animals and plants placed in a closed environment (eg, livestock shed, breeding shed, aquarium, pond). Cell composition.

[0002]

2. Description of the Related Art Among animals and plants, particularly fishes have the property of digesting and utilizing food very efficiently. For example, a high protein food is almost twice as efficient as an animal in adulthood. Grow up to. And the substance that stimulates the sensation / feeding promotion of fish is said to be protein (amino acid), and the protein requirement is remarkable mainly in young fish, and at least 50% in dry amount,
Also, at least 40% protein is required in adult fish. In addition, it requires amino acids possessed by higher vertebrates higher than fish, and it is said that 20-30% of the fat in the diet can be digested if vitamins are properly prepared.
Furthermore, water-soluble vitamins, especially vitamin C, are required, as in other vertebrates, to stimulate and accelerate the metabolism of fish protein synthesis.

[0003] Conventionally, as a feed (feed) satisfying this type of nutritional requirement, one in which the balance of ingredients such as protein, fat and carbohydrate is taken into consideration, for example, pellet-shaped, granular or flake-shaped feed is used. It had been.

[0004]

However, in the above-mentioned conventional feeds, etc .; the compounding substrate (nutrient) component is dispersed, leading to the phenomenon of eutrophication of water quality and deterioration of water quality; Undigested food residues were found in the excrement, which led to the phenomenon of eutrophication of water quality and deterioration of water quality; since vitamins, especially water-soluble vitamins, were dissolved and dispersed in water, No absorption expected; contact with moisture / humidity must be avoided, handling
It has been pointed out that there are problems such as complicated management; it is difficult to understand the actual input amount due to the large volume of appearance; and the physical intake is not always easy because a large amount of air enters the mouth of the fish. It was

Further, in the conventional bait, when it is used in a large amount in a closed water area such as a pond or an aquarium, since it contains the above-mentioned components (substrate / nutrient) that deteriorate water quality,
It may promote the growth of phytospecific plankton (algae), which is a primary product that uses the component as a nutrient. Therefore, it is necessary to determine the feed amount, feed period (interval), etc. when feeding the feed, taking into account the occurrence of plankton due to the input of the substrate, and it is the actual situation that a certain degree of skill is required when using the feed. However, if this adjustment is incorrect, it will result in an environment unfavorable for fish growth, such as polluting the water quality.

[0006]

SUMMARY OF THE INVENTION The present invention has been made by the present inventors as a result of intensive studies in view of the problems pointed out in the above-mentioned prior art. The gist of the present invention is a bacterial cell composition characterized in that, in addition to conventional nutritional components, bacterial cells of active photosynthetic bacteria are used as a bait ingredient.

[0007] That is, the photosynthetic bacteria used in the composition of the present invention are the Berjay's Manual of Determinating Bacteriology 7th Edition (Bergey's Manua).
l ofDeterminative Bacteriology 7th Edition), it belongs to the family of bacteria shown in Table 1 below, and has a size of 0.5 to 15μ and a growth of about 7.8 to about 8.5.
The pH has a common form / property.

[0008]

[Table 1]

The constitution of the bacterial cell composition of the present invention will be specifically described below.

In the present invention, a photosynthetic bacterium is used, but basically, any photosynthetic bacterium belonging to the family of bacteria listed in Table 1 above can be applied to the composition of the present invention. In addition, even if these photosynthetic bacteria are used in a state in which any two or more kinds are mixed, the activity thereof is not particularly hindered, and a mode like this is also applicable to the present invention.

The above-mentioned composition of the present invention comprises, first of all, a concentrated photosynthetic bacterium obtained by using an ordinary photosynthetic bacterium using a centrifuge or a filtration device, or the concentrated bacterium.
The dried microbial cells obtained by drying under a temperature condition of 00 to 240 ° C. and preparing a powder form are mixed with a raw material for a feed composed of usual nutritional ingredients and granulated. When the concentrated bacteria or dried bacterial cells are mixed with the feed, the optimum constituent content of the fish feed [protein 30 to 60%; fat content 5 to 15%;
Mixing is preferably carried out based on carbohydrates; 10-30]. Further, as the particle size of the composition of the present invention obtained by granulation, depending on the form, considering the dispersion in water and the ease of ingestion of fish, etc., about 50μ ~ about
About 3.2 mm is preferable.

[0012]

[Function] The composition of the present invention employing active photosynthetic bacterial cells, once ingested by fish, about 70% of the bacterial cells are absorbed by the living body, and the remaining about 30% of the bacterial cells are It is excreted outside the body while maintaining its activity. As a result, in addition to the nutritional components of the feed compounded with the bacterial cells, the water-soluble vitamins (vitamin B group, vitamin C, etc.) and useful amino acids contained in the bacterial cell components of the active photosynthetic bacteria can also be added to It became possible to introduce it into the living body (metabolic system). In addition, the bacteria excreted in water while retaining the activity absorb the excrement from the living body dispersed in water as well as the nutrients contained in the food residue for the growth thereof, so that the fish can be kept closed. It is possible to prevent the eutrophication tendency of the water system and purify and maintain the water quality. Such effects can be associated with other closed environments (eg, barns, sheds,
The same thing can be said for ().

[0013]

EXAMPLES Examples of the present invention will be described in more detail below.

Example 1: Preparation of bacterial cell composition First, concentrated bacterial cells of photosynthetic bacteria (PSB: about 1200 ml) or the concentrated bacterial cells were sprayed and heated at a temperature of 200 to 240 ° C. by a spray dryer. The dried bacterial cells obtained by drying under the following conditions and powder were mixed with the raw materials for the feed (fish mill, shrimp mill, etc.) to obtain a particle size of 3.2.
The bacterial cell composition of the present invention obtained by granulating to 50 mm (50-60 mesh)
(100 g). The nutritional content of the composition obtained in this example is as shown in Table 2 below.

[0015]

[Table 2]

Example 2: Water purification effect and nutrient absorption of bacterial cell composition
Assay of yield effect The water purification effect and nutrient absorption effect of the bacterial cell composition of the present invention obtained in Example 1 were assayed according to the following procedure.

[Samples] Samples of the present invention …… Bacterial cell composition obtained in Example 1 Control sample …… Bacterial cell-free feed (tetramine-Kelp dry mixture) [Dose] Frequency of administration: once on the start day of the test (First time only) [Experimental conditions] Water taken from natural pond (pH 9.4)
Two aquariums (3 L capacity) were prepared, each containing two 2-3 cm goldfish. An aeration device was connected to each water tank, and the above-mentioned samples were individually charged into each water tank while supplying air. The water temperature at the beginning of the experiment was 25 ° C, and no temperature control was performed thereafter.

Experiments were carried out under the above conditions, and the change in water quality was verified by measuring the total nitrogen and total phosphorus and the transparency of each water tank 4 days after the test was started. The results are shown in Table 3 below.

[0019]

[Table 3]

As is clear from the above results, according to the composition of the present invention, the transparency is remarkably improved as compared with the other samples, and the residual amounts of total nitrogen and total phosphorus are remarkably suppressed, and It was confirmed that the nutritional tendency was significantly suppressed and that the effects of water purification and conservation could be improved. In each of the aquariums, the amount of total nitrogen and total phosphorus increased from the beginning of the experiment is due to the physiological action of the living body (goldfish).

[0021]

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide vitamins (especially water-soluble vitamins) that are easily destroyed by the influence of heat and humidity and are indispensable for the growth of fish, in vivo (metabolism) of the fish through the fungus body. System) and satisfy the nutritional requirements of fish, and since the bacteria that maintain the activity excreted in water absorb excess nutrients in water,
It has the effect of suppressing the eutrophication tendency of closed water systems where fish are placed, suppressing the growth of plankton, and eventually purifying and conserving water quality.

Further, since the administered feed ingredient is efficiently digested by fish and photosynthetic bacteria and the algae removal work is also reduced, the feed cost and maintenance / management burden can be reduced. The effect is also played together.

Claims (3)

[Claims] 1. A bacterial cell composition comprising a preparation comprising photosynthetic bacteria and a nutrient component. 2. The bacterial cell composition according to claim 1, wherein the bacterial cell composition is in one or more forms selected from the group consisting of powder, granules, granules and pellets. 3. The photosynthetic bacterium is an acioloderche (A
thiorhodaceae), Shiorodache (Thiorhodaceae)
Family and Chloro-bacteriace
The bacterial cell composition according to claim 1 or 2, which is a bacterium belonging to one family selected from the group consisting of ae) families.