JPH07132080A - Cell composition - Google Patents
Cell compositionInfo
- Publication number
- JPH07132080A JPH07132080A JP5280854A JP28085493A JPH07132080A JP H07132080 A JPH07132080 A JP H07132080A JP 5280854 A JP5280854 A JP 5280854A JP 28085493 A JP28085493 A JP 28085493A JP H07132080 A JPH07132080 A JP H07132080A
- Authority
- JP
- Japan
- Prior art keywords
- water
- cell composition
- fishes
- feed
- fish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、動植物用飼料として好
適な菌体組成物、特に、閉鎖的環境(例えば、畜舎、飼
育小屋、水槽、池など)におかれた動植物への適用に有
用な菌体組成物に関する。INDUSTRIAL APPLICABILITY The present invention is useful for application to a fungal composition suitable as a feed for animals and plants, particularly animals and plants placed in a closed environment (eg, livestock shed, breeding shed, aquarium, pond). Cell composition.
【0002】[0002]
【従来の技術】動植物の内、特に、魚類は、餌を非常に
効率的に消化・利用する性質があり、例えば、高蛋白の
餌であれば、概ね家畜の2倍程度の効率で成体にまで成
長する。 そして、魚類の感覚・摂餌促進を刺激する物
質は、蛋白質(アミノ酸)とされており、その蛋白要求
は、主に若魚で顕著で、乾燥量にして少なくとも50%、
また、成魚でも少なくとも40%の蛋白が必要とされてい
る。 加えて、魚類より上位の高等脊椎動物が保有する
アミノ酸を必要とし、ビタミン類が適切に揃っておれ
ば、餌中の20〜30%の脂肪を消化できるとされている。
さらに、魚類の蛋白合成の代謝を刺激・促進するため
に、他の脊椎動物と同様、水溶性ビタミン、特に、ビタ
ミンCが必要とされている。2. Description of the Related Art Among animals and plants, particularly fishes have the property of digesting and utilizing food very efficiently. For example, a high protein food is almost twice as efficient as an animal in adulthood. Grow up to. And the substance that stimulates the sensation / feeding promotion of fish is said to be protein (amino acid), and the protein requirement is remarkable mainly in young fish, and at least 50% in dry amount,
Also, at least 40% protein is required in adult fish. In addition, it requires amino acids possessed by higher vertebrates higher than fish, and it is said that 20-30% of the fat in the diet can be digested if vitamins are properly prepared.
Furthermore, water-soluble vitamins, especially vitamin C, are required, as in other vertebrates, to stimulate and accelerate the metabolism of fish protein synthesis.
【0003】そして、従来より、この種の栄養要求を満
たす餌料(飼料)として、蛋白質、脂肪分および炭水化
物等の成分バランスを考慮したもの、例えば、ペレット
状、粒状、あるいはフレーク状の餌料が使用されてい
た。[0003] Conventionally, as a feed (feed) satisfying this type of nutritional requirement, one in which the balance of ingredients such as protein, fat and carbohydrate is taken into consideration, for example, pellet-shaped, granular or flake-shaped feed is used. It had been.
【0004】[0004]
【発明が解決しようとする課題】しかしながら、上記従
来の餌料などでは; 配合基質(栄養)成分が分散し、水質の富栄養化現
象ならびに水質の悪化を招き; 餌料の消化吸収が悪く、生体の排泄物に餌料の未消
化残渣が認められ、これからして、水質の富栄養化現象
ならびに水質の悪化を招き; ビタミン類、特に水溶性ビタミンが水中に溶解・分
散してしまうため、生体への吸収が期待できず; 水分・湿気との接触を避けねばならず、その取扱・
管理が煩雑であること; 見た目の量感が大きいため、実際の投入量がわかり
にくい;および 魚の口中に空気が大量に入るため、その物理的な摂
取が必ずしも容易でないなどの問題点が指摘されてい
た。However, in the above-mentioned conventional feeds, etc .; the compounding substrate (nutrient) component is dispersed, leading to the phenomenon of eutrophication of water quality and deterioration of water quality; Undigested food residues were found in the excrement, which led to the phenomenon of eutrophication of water quality and deterioration of water quality; since vitamins, especially water-soluble vitamins, were dissolved and dispersed in water, No absorption expected; contact with moisture / humidity must be avoided, handling
It has been pointed out that there are problems such as complicated management; it is difficult to understand the actual input amount due to the large volume of appearance; and the physical intake is not always easy because a large amount of air enters the mouth of the fish. It was
【0005】さらに、従来の餌料では、上記したような
水質を悪化させる(基質・栄養)成分を含んでいる関係
から、池、水槽等の閉鎖系水域で大量に使用した場合、
当該成分を栄養源とする一次生産物である植物性特定プ
ランクトン(藻類)の生育を促進しかねない。 従っ
て、基質の投入によるプランクトン発生などを考慮に入
れて、餌料投入時にその投入量、投入期間(間隔)等を
定めなければならず、その使用に際してはある程度の熟
練を要求されるのが実情であり、この加減を誤ると水質
を汚染するなどの魚類の生育にとって好ましくない環境
をもたらす結果となるものであった。Further, in the conventional bait, when it is used in a large amount in a closed water area such as a pond or an aquarium, since it contains the above-mentioned components (substrate / nutrient) that deteriorate water quality,
It may promote the growth of phytospecific plankton (algae), which is a primary product that uses the component as a nutrient. Therefore, it is necessary to determine the feed amount, feed period (interval), etc. when feeding the feed, taking into account the occurrence of plankton due to the input of the substrate, and it is the actual situation that a certain degree of skill is required when using the feed. However, if this adjustment is incorrect, it will result in an environment unfavorable for fish growth, such as polluting the water quality.
【0006】[0006]
【課題を解決するための手段】本発明は、上述した従来
技術にて指摘されていた問題点に鑑みて、本発明者が鋭
意研究を重ねた結果、発明するに至ったものであり、そ
の要旨とするところは、餌料成分として、従来の栄養成
分に加えて、活性光合成細菌の菌体を採用したことを特
徴とする菌体組成物である。SUMMARY OF THE INVENTION The present invention has been made by the present inventors as a result of intensive studies in view of the problems pointed out in the above-mentioned prior art. The gist of the present invention is a bacterial cell composition characterized in that, in addition to conventional nutritional components, bacterial cells of active photosynthetic bacteria are used as a bait ingredient.
【0007】すなわち、本発明組成物にて使用する光合
成細菌とは、バージェイズ・マニュアル・オブ・デター
ミナテイブ・バクテリオロジイ第7版(Bergey's Manua
l ofDeterminative Bacteriology 7th Edition) によれ
ば、下記表1に示した細菌科に属するものであり、ま
た、 0.5〜15μの大きさ、および約 7.8〜約 8.5の生育
pHが、共通する形態・性状とされているものである。[0007] That is, the photosynthetic bacteria used in the composition of the present invention are the Berjay's Manual of Determinating Bacteriology 7th Edition (Bergey's Manua).
l ofDeterminative Bacteriology 7th Edition), it belongs to the family of bacteria shown in Table 1 below, and has a size of 0.5 to 15μ and a growth of about 7.8 to about 8.5.
The pH has a common form / property.
【0008】[0008]
【表1】 [Table 1]
【0009】以下に、本発明の菌体組成物について、具
体的にその構成を説明する。The constitution of the bacterial cell composition of the present invention will be specifically described below.
【0010】本発明では、光合成細菌を使用するが、基
本的には前出の表1に列挙した細菌科に属する光合成細
菌であれば、本願発明組成物に適用可能である。 な
お、これら光合成細菌は、任意の二種以上を混在させた
状態で使用しても、その活性に別段の支障は無く、しか
るにかような態様も本願発明では適用可能である。In the present invention, a photosynthetic bacterium is used, but basically, any photosynthetic bacterium belonging to the family of bacteria listed in Table 1 above can be applied to the composition of the present invention. In addition, even if these photosynthetic bacteria are used in a state in which any two or more kinds are mixed, the activity thereof is not particularly hindered, and a mode like this is also applicable to the present invention.
【0011】上述した本発明の組成物は、まず、通常の
光合成細菌を遠心分離装置または濾過装置を使用して取
得した光合成細菌の濃縮細菌、あるいは該濃縮細菌を 2
00〜240℃の温度条件下で乾燥し、粉末状に調製して得
られた乾燥菌体を、通常の栄養成分からなる餌料の原材
料と共に混合して、造粒する。 なお、濃縮細菌あるい
は乾燥菌体と、餌料との混合に際しては、魚類用餌料の
至適構成成分含量〔蛋白質30〜60%;脂肪分5〜15%;
炭水化物;10〜30〕を基準にして混合を行うのが好まし
い。 また、造粒して得られた本発明組成物の粒径とし
ては、その形態によっても異なるが、水中での分散なら
びに魚の摂取のしやすさなどを考慮すれば、約50μ〜約
3.2mm 程度が好ましい。The above-mentioned composition of the present invention comprises, first of all, a concentrated photosynthetic bacterium obtained by using an ordinary photosynthetic bacterium using a centrifuge or a filtration device, or the concentrated bacterium.
The dried microbial cells obtained by drying under a temperature condition of 00 to 240 ° C. and preparing a powder form are mixed with a raw material for a feed composed of usual nutritional ingredients and granulated. When the concentrated bacteria or dried bacterial cells are mixed with the feed, the optimum constituent content of the fish feed [protein 30 to 60%; fat content 5 to 15%;
Mixing is preferably carried out based on carbohydrates; 10-30]. Further, as the particle size of the composition of the present invention obtained by granulation, depending on the form, considering the dispersion in water and the ease of ingestion of fish, etc., about 50μ ~ about
About 3.2 mm is preferable.
【0012】[0012]
【作用】活性光合成細菌の菌体を採用した本発明組成物
は、一旦、魚により摂取されれば、その約70%の菌体が
生体に吸収され、残りの約30%の菌体が、生体外に活性
を保った状態で排出される。 これにより、菌体と共に
配合された餌料の栄養成分に加え、活性光合成細菌の菌
体成分に含まれる水溶性ビタミン(ビタミンB群、ビタ
ミンCなど)および有用アミノ酸をも、菌体を介して魚
の生体内(代謝系)へ導入することが可能となったので
ある。 さらに、活性を保持した状態で、水中へ排出さ
れた菌体は、その成育のために、水中に分散した生体か
らの排泄物ならびに餌料残渣に含まれる栄養素を吸収す
るので、魚の置かれた閉鎖水系の富栄養化傾向の抑止な
らびに水質の浄化・保全が図れるのである。 このよう
な作用は、他の閉鎖的環境(例えば、畜舎、飼育小屋、
など)についても、同様のことが言える。[Function] The composition of the present invention employing active photosynthetic bacterial cells, once ingested by fish, about 70% of the bacterial cells are absorbed by the living body, and the remaining about 30% of the bacterial cells are It is excreted outside the body while maintaining its activity. As a result, in addition to the nutritional components of the feed compounded with the bacterial cells, the water-soluble vitamins (vitamin B group, vitamin C, etc.) and useful amino acids contained in the bacterial cell components of the active photosynthetic bacteria can also be added to It became possible to introduce it into the living body (metabolic system). In addition, the bacteria excreted in water while retaining the activity absorb the excrement from the living body dispersed in water as well as the nutrients contained in the food residue for the growth thereof, so that the fish can be kept closed. It is possible to prevent the eutrophication tendency of the water system and purify and maintain the water quality. Such effects can be associated with other closed environments (eg, barns, sheds,
The same thing can be said for ().
【0013】[0013]
【実施例】以下、本発明の実施例についてより詳細に説
明する。EXAMPLES Examples of the present invention will be described in more detail below.
【0014】実施例1:菌体組成物の調製 まず、光合成細菌(PSB:約1200ml)の濃縮菌体、あ
るいは該濃縮菌体を噴霧加熱装置(スプレイドライア
ー)にて 200〜 240℃の温度条件下で乾燥し、粉末状に
調製して得られた乾燥菌体を、餌料の原材料(フィシュ
ミル、シュリンプミルなど)と共に混合して、粒径 3.2
mm (50〜60メッシュ) に造粒して本願発明の菌体組成物
(100g)とした。 なお、本実施例で得られた組成物の栄
養成分含量は、下記表2に示した通りである。 Example 1: Preparation of bacterial cell composition First, concentrated bacterial cells of photosynthetic bacteria (PSB: about 1200 ml) or the concentrated bacterial cells were sprayed and heated at a temperature of 200 to 240 ° C. by a spray dryer. The dried bacterial cells obtained by drying under the following conditions and powder were mixed with the raw materials for the feed (fish mill, shrimp mill, etc.) to obtain a particle size of 3.2.
The bacterial cell composition of the present invention obtained by granulating to 50 mm (50-60 mesh)
(100 g). The nutritional content of the composition obtained in this example is as shown in Table 2 below.
【0015】[0015]
【表2】 [Table 2]
【0016】実施例2:菌体組成物の浄水効果・栄養吸
収効果の検定 実施例1にて得られた本願発明の菌体組成物の浄水効果
および栄養吸収効果につき、下記手順に従って検定し
た。 Example 2: Water purification effect and nutrient absorption of bacterial cell composition
Assay of yield effect The water purification effect and nutrient absorption effect of the bacterial cell composition of the present invention obtained in Example 1 were assayed according to the following procedure.
【0017】〔試料〕 本発明試料 …… 実施例1にて得られた菌体組成物 対照試料 …… 菌体無配合餌料(テトラミン・ケル
プドライ混合物) 〔用量〕 投与頻度:試験開始日の1回(初回)のみ 〔実験条件〕自然池から採取した水(pH 9.4)
を張った、2〜3cm級の金魚各2尾を入れた2つの水槽
(3L容)を用意した。 各水槽にエアレーション装置
を接続して、給気しながら、各水槽に上記試料を個別に
投入した。 なお、実験開始当初の水温は、25℃で、そ
の後の温度調節は一切行わなかった。[Samples] Samples of the present invention …… Bacterial cell composition obtained in Example 1 Control sample …… Bacterial cell-free feed (tetramine-Kelp dry mixture) [Dose] Frequency of administration: once on the start day of the test (First time only) [Experimental conditions] Water taken from natural pond (pH 9.4)
Two aquariums (3 L capacity) were prepared, each containing two 2-3 cm goldfish. An aeration device was connected to each water tank, and the above-mentioned samples were individually charged into each water tank while supplying air. The water temperature at the beginning of the experiment was 25 ° C, and no temperature control was performed thereafter.
【0018】上記条件下にて実験を行い、試験を開始し
てから4日後の各水槽の総窒素・総リン、および透明度
を測定して水質の変化を検証した。 その結果を、下記
表3に示した。Experiments were carried out under the above conditions, and the change in water quality was verified by measuring the total nitrogen and total phosphorus and the transparency of each water tank 4 days after the test was started. The results are shown in Table 3 below.
【0019】[0019]
【表3】 [Table 3]
【0020】上記結果から明らかな通り、本願発明の組
成物によると、他の試料と比較して透明度の改善が格段
に優れ、また、総窒素および総リンの残量が顕著に抑制
され、富栄養化傾向が有為に抑制されると共に、水質浄
化および保全の効果が改善できることが確認されたので
ある。 なお、いずれの水槽でも、実験開始当初よりも
総窒素および総リンの量が増えているのは、生体(金
魚)の生理作用によるものである。As is clear from the above results, according to the composition of the present invention, the transparency is remarkably improved as compared with the other samples, and the residual amounts of total nitrogen and total phosphorus are remarkably suppressed, and It was confirmed that the nutritional tendency was significantly suppressed and that the effects of water purification and conservation could be improved. In each of the aquariums, the amount of total nitrogen and total phosphorus increased from the beginning of the experiment is due to the physiological action of the living body (goldfish).
【0021】[0021]
【発明の効果】本発明により、熱・湿度の影響によって
破壊されやすく、かつ魚類の成長に欠かせないビタミン
類(特に、水溶性ビタミン)の提供が、菌体を介して魚
の生体内(代謝系)へ導入することが可能となり、魚類
の栄養要求を満足せしめる上に、水中へ排出された活性
を維持した菌体が水中の余分な栄養素を吸収するので、
魚の置かれた閉鎖水系の富栄養化傾向の抑止ならびにプ
ランクトン増殖の抑止、ひいては水質の浄化・保全が図
れるなどの効果を奏するものである。INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide vitamins (especially water-soluble vitamins) that are easily destroyed by the influence of heat and humidity and are indispensable for the growth of fish, in vivo (metabolism) of the fish through the fungus body. System) and satisfy the nutritional requirements of fish, and since the bacteria that maintain the activity excreted in water absorb excess nutrients in water,
It has the effect of suppressing the eutrophication tendency of closed water systems where fish are placed, suppressing the growth of plankton, and eventually purifying and conserving water quality.
【0022】また、投与した餌料成分が、魚類ならびに
光合成細菌によって効率良く消化され、また、藻類の除
去作業も軽減されるため、餌料に要する費用ならびに保
守・管理の面の負担を節減できるなどの効果も、併せて
奏するものである。Further, since the administered feed ingredient is efficiently digested by fish and photosynthetic bacteria and the algae removal work is also reduced, the feed cost and maintenance / management burden can be reduced. The effect is also played together.
Claims (3)
を含むことを特徴とする菌体組成物。1. A bacterial cell composition comprising a preparation comprising photosynthetic bacteria and a nutrient component.
よびペレットからなるグループから選択された一つ以上
の形態である請求項1に記載の菌体組成物。2. The bacterial cell composition according to claim 1, wherein the bacterial cell composition is in one or more forms selected from the group consisting of powder, granules, granules and pellets.
thiorhodaceae)科、シオロダーシェ(Thiorhodaceae)
科、およびクロロバクテリアーシェ(Chloro-bacteriace
ae)科からなるグループから選択された一つの科に属す
る細菌である請求項1もしくは2に記載の菌体組成物。3. The photosynthetic bacterium is an acioloderche (A
thiorhodaceae), Shiorodache (Thiorhodaceae)
Family and Chloro-bacteriace
The bacterial cell composition according to claim 1 or 2, which is a bacterium belonging to one family selected from the group consisting of ae) families.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5280854A JPH07132080A (en) | 1993-11-10 | 1993-11-10 | Cell composition |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5280854A JPH07132080A (en) | 1993-11-10 | 1993-11-10 | Cell composition |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH07132080A true JPH07132080A (en) | 1995-05-23 |
Family
ID=17630909
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5280854A Pending JPH07132080A (en) | 1993-11-10 | 1993-11-10 | Cell composition |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH07132080A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1313395C (en) * | 2005-03-09 | 2007-05-02 | 西北农林科技大学 | Super-concentrated algae bacterium micro-ecological balance suspension type water quality modifier and preparation method thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54145280A (en) * | 1978-05-04 | 1979-11-13 | Toho Rayon Co Ltd | Production of cells of photosynthetic bacterium |
JPS54151186A (en) * | 1978-05-16 | 1979-11-28 | Kirin Shiiguramu Kk | Production of cell bodies of phototrophic bacteria |
-
1993
- 1993-11-10 JP JP5280854A patent/JPH07132080A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54145280A (en) * | 1978-05-04 | 1979-11-13 | Toho Rayon Co Ltd | Production of cells of photosynthetic bacterium |
JPS54151186A (en) * | 1978-05-16 | 1979-11-28 | Kirin Shiiguramu Kk | Production of cell bodies of phototrophic bacteria |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1313395C (en) * | 2005-03-09 | 2007-05-02 | 西北农林科技大学 | Super-concentrated algae bacterium micro-ecological balance suspension type water quality modifier and preparation method thereof |
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