JPH0687711B2 - Nutrients for artificial cultivation of mushrooms - Google Patents

Description

TECHNICAL FIELD The present invention relates to a nutrient for artificial cultivation of mushrooms, and more specifically, it can be cultivated in a short period of time when artificially cultivating mushrooms, especially nameko, shimeji mushrooms, and oyster mushrooms. Moreover, the present invention relates to a nutrient for artificial cultivation of mushrooms, which can increase the yield.

[Prior Art] Various techniques for artificial culture medium for mushrooms (nameko, shimeji mushrooms, oyster mushrooms, etc.) and nutrients have been conventionally known.

That is, the so-called Distillers Solubles, which is a generic term for those dissolved in water except for coarse particles of particles present in distilled waste liquid such as whiskey, or suspended, is widely used as a nutrient for products such as microorganisms. ing. Further, it has been proposed that the product containing the active ingredient of Distillers Solubles is particularly suitable for use in the growth of bacterial cells. (See Japanese Patent Publication No. 57-56352) Similarly, for artificial cultivation of shiitake mushrooms, it is known to use an artificial medium containing a distillation residue of whiskey or a concentrate thereof and having a pH adjusted to about 5. There is. (Japanese Patent Publication Sho 61-4
[Problems to be Solved by the Invention] However, many of the conventional nutrients for artificial cultivation of mushrooms described above have a pH of about 4 and when mixed with a medium, mushrooms Lower than the optimum pH of
There was a drawback that the yield or its morphology was slightly deformed.

[Means for Solving Problems] Therefore, the inventors of the present invention have diligently studied in order to solve the above-mentioned problems, and as a result, calcium carbonate, which has been used in an extremely small amount for conventional pH adjustment, Not a small amount
It was found that, when a considerable amount of 10 to 30% by weight is used, not only surprisingly the yield of mushrooms is large and the number of cultivation days can be shortened, but also the form becomes a nutritional agent with almost no malformation, and the present invention was reached. It was done.

That is, according to the present invention, either one of the dried product of maize lees and the dried product of whiskey lees and a concentrated liquid of whiskey distillation waste liquid are mixed, and 70 to 90% by weight of this mixture, 10 to 30% by weight of calcium carbonate. There is provided a nutrient for artificial cultivation of mushrooms, which comprises:

In the present invention, the main component is a mixture of either a dried product of maize dregs or a dried product of whiskey dregs and a concentrated liquid of whiskey distilling waste liquid. Here, the concentrated liquid of the whiskey distillation waste liquid usually contains water of 60 to 70% by weight. The above mixture is a dried product of maize dregs or a dried product of whiskey dregs and a concentrated liquid of whiskey distilling waste liquid (water content: 60%).
˜70% by weight) in a weight ratio of about 1: 2 to 2: 1, preferably 1: 1.

Incidentally, here, the maize meal is a by-product at the time of grain whiskey production, and is a residue obtained by mixing the steamed corn and malt of barley and filtering this after saccharification, and whiskey meal is It is a by-product in the production of malt whiskey and refers to the residue (malt meal) obtained by crushing malt, charging it with warm water, saccharifying and filtering.

In the present invention, calcium carbonate is added to the above mixture, but the mixing ratio is 70 to 90% by weight of the mixture.
And 10 to 30% by weight of calcium carbonate, preferably about 80% by weight of the mixture and about 20% by weight of calcium carbonate.

[Examples] The present invention will be described in more detail based on the following examples, but it should be clear that the present invention is not limited to these examples.

(Example 1, Comparative Example 1) The nutritional supplement having the following composition was obtained by mixing and drying a dried product of maize lees and a concentrated liquid of whiskey distillation waste liquid at a weight ratio of 1: 1 (hereinafter referred to as A). .) And calcium carbonate. Here, the mixing ratio of A and calcium carbonate was 8: 2 by weight.

That is, 20 g of calcium carbonate in 100 g of this nutritional supplement.
(That is, 20% by weight). Its composition was as follows.

The content of crude ash in the above table is approximately the same as the amount of calcium carbonate blended in the nutritional supplement, but it is 20% by weight depending on the small amount of crude ash contained in A and the purity of the calcium carbonate raw material to be contained. However, it was 20.4% by weight.

In this example, oyster mushrooms were cultivated as mushrooms. Comparative Example 1 was prepared by mixing an ogre: nutrient base material in a volume ratio of 2: 1. Of these, 40% by weight of the nutrient base material was compared with a nutrient solution having the above composition (Example 1).

An 800 cc container was filled with 480 g of rice bran as a nutrient base material, adjusted to have a water content of 65% by weight, and then sterilized at 120 ° C. for 90 minutes. The next day, inoculate the inoculum, incubate at 20 ° C for 7 days, then bring it to 23 ° C, and depending on the difference in the completion of hyphal elongation, fungi were picked on the 28th day and the 32nd day, and it was generated at 12 ° C and humidity of 90% or higher .

Tohoku H-65 and H-66 (sold by Tohoku Shiitake Co., Ltd.) were used as inoculum. (N = 12) The results are shown below.

(Examples 2 to 3) The nutritional supplements having the following compositions were obtained by mixing and drying a dried product of whiskey lees and a concentrated liquid of whiskey distillation waste liquid in a weight ratio of 1: 1 (DDMS) and calcium carbonate. It is a mixture. Here, the mixing ratio of DDMS and calcium carbonate was 8: 2 by weight.

That is, 20 g of calcium carbonate in 100 g of this nutritional supplement.
(That is, 20% by weight). Its composition is shown below.

The content of crude ash in the above table was 23.6% by weight instead of 20% by weight depending on the purity of the crude ash contained in DDMS and the calcium carbonate raw material to be contained.

Under the same conditions as in Example 1 above, except that 800 g of container was filled with 450 g of bran as a nutrient base material (Example 2) and 480 g of rice bran was used (Example 3). Pleurotus ostreatus was cultivated.

The results are shown below.

As is clear from the results of Examples, it is understood that the yield when the nutritional supplement of the present invention is used is higher than in the conventional case and the number of harvest days is shortened.

[Effects of the Invention] As described above, the nutrient for artificial cultivation of mushrooms of the present invention is prepared by mixing either one of the dried product of maize lees and the dried product of whiskey lees and a concentrated liquid of whiskey distillation waste liquid. 70-90% by weight of the mixture, 10-30% by weight of calcium carbonate
Since it has been added, it has the effects of increasing the amount of harvest, shortening the number of harvest days, and providing mushrooms in good form.

Claims (2)

[Claims] 1. A dry matter of maize dregs or a whiskey dregs, and a concentrated liquid of whiskey distilling waste liquid are mixed, and 70 to 90% by weight of this mixture is mixed with 10 to 30 calcium carbonate.
A nutritional supplement for artificial cultivation of mushrooms, which is blended with wt%. 2. The concentrated liquid of whiskey distilling waste liquid has a water content of 60 to 70.
A nutritional supplement for artificial cultivation of mushrooms according to claim 1, which comprises a weight percentage.