JPH067165A - わずかに誘導または抑制されたｍＲＮＡの検出を可能にする改善された弁別スクリーニング法 - Google Patents

わずかに誘導または抑制されたｍＲＮＡの検出を可能にする改善された弁別スクリーニング法

Info

Publication number
JPH067165A
JPH067165A JP5061113A JP6111393A JPH067165A JP H067165 A JPH067165 A JP H067165A JP 5061113 A JP5061113 A JP 5061113A JP 6111393 A JP6111393 A JP 6111393A JP H067165 A JPH067165 A JP H067165A
Authority
JP
Japan
Prior art keywords
probe
mrna
untreated
treated
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP5061113A
Other languages
English (en)
Japanese (ja)
Inventor
Stephanie Jean Lemke
ステファニー・ジーン・レムケ
Robert Earl Moore Jr
ロバート・アール・ムーア・ジュニア
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of JPH067165A publication Critical patent/JPH067165A/ja
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP5061113A 1992-02-28 1993-02-24 わずかに誘導または抑制されたｍＲＮＡの検出を可能にする改善された弁別スクリーニング法 Withdrawn JPH067165A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84339892A 1992-02-28 1992-02-28
US843398 1992-02-28

Publications (1)

Publication Number Publication Date
JPH067165A true JPH067165A (ja) 1994-01-18

Family

ID=25289854

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5061113A Withdrawn JPH067165A (ja) 1992-02-28 1993-02-24 わずかに誘導または抑制されたｍＲＮＡの検出を可能にする改善された弁別スクリーニング法

Country Status (5)

Country Link
EP (1) EP0558290A2 (enExample)
JP (1) JPH067165A (enExample)
CA (1) CA2089045A1 (enExample)
IL (1) IL104651A0 (enExample)
MX (1) MX9300703A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69535428T2 (de) * 1994-02-14 2007-12-06 Smithkline Beecham Corp. Verfahren zum Auffinden von differentiel exprimierte Gene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007585A1 (en) * 1987-04-02 1988-10-06 Genelabs, Incorporated Equal-abundance transcript composition and method

Also Published As

Publication number Publication date
EP0558290A2 (en) 1993-09-01
CA2089045A1 (en) 1993-08-29
EP0558290A3 (enExample) 1995-08-09
MX9300703A (es) 1993-08-01
IL104651A0 (en) 1993-06-10

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Legal Events

Date Code Title Description
A300 Application deemed to be withdrawn because no request for examination was validly filed

Free format text: JAPANESE INTERMEDIATE CODE: A300

Effective date: 20000509