JPH06507971A - 絶対白血球サブセット計数値および白血球の多数の部の差を得るための方法および装置 - Google Patents
絶対白血球サブセット計数値および白血球の多数の部の差を得るための方法および装置Info
- Publication number
- JPH06507971A JPH06507971A JP5500244A JP50024493A JPH06507971A JP H06507971 A JPH06507971 A JP H06507971A JP 5500244 A JP5500244 A JP 5500244A JP 50024493 A JP50024493 A JP 50024493A JP H06507971 A JPH06507971 A JP H06507971A
- Authority
- JP
- Japan
- Prior art keywords
- microspheres
- cells
- sample
- cell
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 93
- 210000000265 leukocyte Anatomy 0.000 title claims description 49
- 210000004027 cell Anatomy 0.000 claims description 290
- 239000004005 microsphere Substances 0.000 claims description 221
- 239000000203 mixture Substances 0.000 claims description 66
- 239000000376 reactant Substances 0.000 claims description 66
- 210000004369 blood Anatomy 0.000 claims description 61
- 239000008280 blood Substances 0.000 claims description 61
- 239000000427 antigen Substances 0.000 claims description 46
- 102000036639 antigens Human genes 0.000 claims description 46
- 108091007433 antigens Proteins 0.000 claims description 46
- 230000003287 optical effect Effects 0.000 claims description 44
- 238000002156 mixing Methods 0.000 claims description 19
- 210000000440 neutrophil Anatomy 0.000 claims description 19
- 230000001413 cellular effect Effects 0.000 claims description 13
- 238000012216 screening Methods 0.000 claims description 12
- 239000002738 chelating agent Substances 0.000 claims description 6
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 claims description 5
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 claims description 5
- 230000002708 enhancing effect Effects 0.000 claims 4
- 230000002596 correlated effect Effects 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 description 91
- 210000003743 erythrocyte Anatomy 0.000 description 39
- 210000004698 lymphocyte Anatomy 0.000 description 19
- 102100036011 T-cell surface glycoprotein CD4 Human genes 0.000 description 15
- 238000010186 staining Methods 0.000 description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 12
- 239000003795 chemical substances by application Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 11
- 210000000601 blood cell Anatomy 0.000 description 10
- 238000010586 diagram Methods 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 230000002934 lysing effect Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 6
- 210000000805 cytoplasm Anatomy 0.000 description 6
- 239000008187 granular material Substances 0.000 description 6
- 208000032839 leukemia Diseases 0.000 description 6
- 210000000207 lymphocyte subset Anatomy 0.000 description 6
- 210000001744 T-lymphocyte Anatomy 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 230000002776 aggregation Effects 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000004069 differentiation Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000005755 formation reaction Methods 0.000 description 5
- 238000000386 microscopy Methods 0.000 description 5
- 210000005259 peripheral blood Anatomy 0.000 description 5
- 239000011886 peripheral blood Substances 0.000 description 5
- 108020003175 receptors Proteins 0.000 description 5
- 102000005962 receptors Human genes 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 238000004220 aggregation Methods 0.000 description 4
- 210000001185 bone marrow Anatomy 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000000834 fixative Substances 0.000 description 4
- 238000000684 flow cytometry Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 239000002826 coolant Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 210000001616 monocyte Anatomy 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- VEMKTZHHVJILDY-UHFFFAOYSA-N resmethrin Chemical compound CC1(C)C(C=C(C)C)C1C(=O)OCC1=COC(CC=2C=CC=CC=2)=C1 VEMKTZHHVJILDY-UHFFFAOYSA-N 0.000 description 3
- 239000000052 vinegar Substances 0.000 description 3
- 235000021419 vinegar Nutrition 0.000 description 3
- 208000010839 B-cell chronic lymphocytic leukemia Diseases 0.000 description 2
- 108010041397 CD4 Antigens Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000004970 cd4 cell Anatomy 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000171 quenching effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010000830 Acute leukaemia Diseases 0.000 description 1
- 241000256844 Apis mellifera Species 0.000 description 1
- 102000013925 CD34 antigen Human genes 0.000 description 1
- 108050003733 CD34 antigen Proteins 0.000 description 1
- 210000004366 CD4-positive T-lymphocyte Anatomy 0.000 description 1
- 241000189662 Calla Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- YASYEJJMZJALEJ-UHFFFAOYSA-N Citric acid monohydrate Chemical group O.OC(=O)CC(O)(C(O)=O)CC(O)=O YASYEJJMZJALEJ-UHFFFAOYSA-N 0.000 description 1
- 108010065556 Drug Receptors Proteins 0.000 description 1
- 102000013138 Drug Receptors Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000901143 Etia Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000002738 Giemsa staining Methods 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 102000009465 Growth Factor Receptors Human genes 0.000 description 1
- 108010009202 Growth Factor Receptors Proteins 0.000 description 1
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 1
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 1
- 102100025304 Integrin beta-1 Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000003729 Neprilysin Human genes 0.000 description 1
- 108090000028 Neprilysin Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241001510071 Pyrrhocoridae Species 0.000 description 1
- 102220493485 Sodium/calcium exchanger 3_N16A_mutation Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000001420 bacteriolytic effect Effects 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000003966 growth inhibitor Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 108091008039 hormone receptors Proteins 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000005435 mesosphere Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical group O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007430 reference method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/0606—Investigating concentration of particle suspensions by collecting particles on a support
- G01N15/0618—Investigating concentration of particle suspensions by collecting particles on a support of the filter type
- G01N15/0625—Optical scan of the deposits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N2015/1472—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle with colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Dispersion Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/703,163 US5348859A (en) | 1990-11-23 | 1991-05-20 | Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential |
US703.163 | 1991-05-20 | ||
PCT/US1992/004152 WO1992021027A1 (en) | 1991-05-20 | 1992-05-18 | Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH06507971A true JPH06507971A (ja) | 1994-09-08 |
Family
ID=24824285
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5500244A Pending JPH06507971A (ja) | 1991-05-20 | 1992-05-18 | 絶対白血球サブセット計数値および白血球の多数の部の差を得るための方法および装置 |
Country Status (9)
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1992009878A1 (en) * | 1990-11-23 | 1992-06-11 | Coulter Corporation | Method and apparatus for optically screening microscopic cells |
US6682940B2 (en) | 1999-05-04 | 2004-01-27 | Dan A. Pankowsky | Products and methods for single parameter and multiparameter phenotyping of cells |
DE60031521T2 (de) * | 1999-05-04 | 2007-06-21 | Dan A. Nashville Pankowsky | Produkte und verfahren für einzelwert- und vielfachwert-phänotypisierung von zellen |
US6828157B1 (en) * | 1999-05-04 | 2004-12-07 | Dan A. Pankowsky | Products and methods for single parameter and multiparameter phenotyping of cells |
AU2002241602A1 (en) * | 2000-11-16 | 2002-06-11 | Burstein Technologies, Inc. | Methods and apparatus for detecting and quantifying lymphocytes with optical biodiscs |
US20030143637A1 (en) * | 2001-08-31 | 2003-07-31 | Selvan Gowri Pyapali | Capture layer assemblies for cellular assays including related optical analysis discs and methods |
JP2005502872A (ja) * | 2001-09-07 | 2005-01-27 | バースタイン テクノロジーズ,インコーポレイティド | 光バイオディスクシステムを使用した、核の形態に基づく白血球の型の識別および定量 |
WO2003044481A2 (en) * | 2001-11-20 | 2003-05-30 | Burstein Technologies, Inc. | Optical bio-discs and microfluidic devices for analysis of cells |
US7141369B2 (en) * | 2002-04-25 | 2006-11-28 | Semibio Technology, Inc. | Measuring cellular metabolism of immobilized cells |
CA2489176C (en) * | 2002-06-11 | 2013-10-15 | Chempaq A/S | Lysing reagent, cartridge and automatic electronic cell counter for simultaneous enumeration of different types of white blood cells |
CA2447586A1 (en) * | 2002-11-01 | 2004-05-01 | Streck Laboratories, Inc. | Improved hematology reagent and methods |
AU2004243690A1 (en) * | 2003-03-03 | 2004-12-09 | Burstein Technologies, Inc. | Methods and apparatus for use in detection and quantitation of various cell types and use of optical bio-disc for performing same |
DE102005052752A1 (de) * | 2005-11-04 | 2007-05-10 | Clondiag Chip Technologies Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
CA2855108A1 (en) * | 2005-02-10 | 2006-08-17 | Koninklijke Philips Electronics N.V. | Dual sample cartridge and method for characterizing particles in liquid |
AU2006212607B2 (en) | 2005-02-10 | 2010-12-09 | Koninklijke Philips Electronics N.V. | Dual sample cartridge and method for characterizing particle in liquid |
US7822276B2 (en) * | 2005-02-17 | 2010-10-26 | Iris International, Inc. | Method and apparatus for analyzing body fluids |
US7738094B2 (en) * | 2007-01-26 | 2010-06-15 | Becton, Dickinson And Company | Method, system, and compositions for cell counting and analysis |
US8331751B2 (en) * | 2009-03-02 | 2012-12-11 | mBio Diagnositcs, Inc. | Planar optical waveguide with core of low-index-of-refraction interrogation medium |
US9212995B2 (en) | 2009-03-02 | 2015-12-15 | Mbio Diagnostics, Inc. | System and method for detecting multiple molecules in one assay |
US9658222B2 (en) | 2009-03-02 | 2017-05-23 | Mbio Diagnostics, Inc. | Planar waveguide based cartridges and associated methods for detecting target analyte |
US10114020B2 (en) | 2010-10-11 | 2018-10-30 | Mbio Diagnostics, Inc. | System and device for analyzing a fluidic sample |
US9523682B2 (en) | 2011-11-16 | 2016-12-20 | Becton, Dickinson And Company | Methods and systems for detecting an analyte in a sample |
EP2911791A4 (en) | 2012-10-29 | 2016-11-02 | Mbio Diagnostics Inc | BIOPARTICLE IDENTIFICATION SYSTEM, CARTRIDGE AND RELATED METHODS |
US9678065B2 (en) | 2013-01-11 | 2017-06-13 | Becton, Dickinson And Company | Low-cost point-of-care assay device |
JP6632525B2 (ja) | 2013-11-06 | 2020-01-22 | ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company | マイクロ流体デバイスならびにその製造方法および使用方法 |
BR112016010721B1 (pt) | 2013-11-13 | 2021-06-01 | Becton, Dickinson And Company | Método e sistema de análise de uma amostra para um analito |
WO2016060793A1 (en) | 2014-10-14 | 2016-04-21 | Becton, Dickinson And Company | Blood sample management using open cell foam |
PL3094252T3 (pl) | 2014-10-14 | 2022-02-21 | Becton, Dickinson And Company | Zarządzanie próbką krwi z zastosowaniem pianki otwartokomórkowej |
CN106604780B (zh) | 2015-03-10 | 2019-06-25 | 贝克顿·迪金森公司 | 生物流体微样本管理装置 |
JP2018536142A (ja) | 2015-09-01 | 2018-12-06 | ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company | 試料の相を分離するためのデプスフィルトレーションデバイス |
WO2017074815A1 (en) | 2015-10-26 | 2017-05-04 | Idexx Laboratories, Inc. | Hematology test slide |
US12403465B2 (en) | 2017-10-11 | 2025-09-02 | Essenlix Corporation | Containing a liquid sample |
CN112763395A (zh) * | 2020-12-28 | 2021-05-07 | 中国科学院东北地理与农业生态研究所 | 一种浮游植物藻类镜检的方法 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4029470A (en) * | 1975-10-29 | 1977-06-14 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Automated single-slide staining device |
US4115535A (en) * | 1977-06-22 | 1978-09-19 | General Electric Company | Diagnostic method employing a mixture of normally separable protein-coated particles |
US4342739A (en) * | 1979-01-09 | 1982-08-03 | Fuji Photo Film Co., Ltd. | Novel material for immunological assay of biochemical components and a process for the determination of said components |
US4615878A (en) * | 1980-03-12 | 1986-10-07 | Lawrence Kass | Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes |
JPS5842971A (ja) * | 1981-09-07 | 1983-03-12 | Fuji Photo Film Co Ltd | 抗体を感作させたマイクロカプセルを用いる細胞性免疫測定法 |
US4511662A (en) * | 1982-06-18 | 1985-04-16 | Bio-Rad Laboratories, Inc. | Simultaneous assay for T and B lymphocyte populations and subpopulations |
JPS60157050A (ja) * | 1984-01-27 | 1985-08-17 | Mitsubishi Rayon Co Ltd | リンパ球t細胞百分率測定方法 |
US4714606A (en) * | 1984-05-15 | 1987-12-22 | Cytocolor Incorporated | Method of staining and identifying cells and compositions thereof |
US4931395A (en) * | 1986-12-08 | 1990-06-05 | Dana-Farber Cancer Institute | Monoclonal antibody specific to neutrophils |
CA1326435C (en) * | 1987-03-13 | 1994-01-25 | Wallace H. Coulter | Method and apparatus for rapid mixing of small volumes for enhancing biological reactions |
US4865971A (en) * | 1987-06-30 | 1989-09-12 | Coulter Corporation | Monoclonal antibody specific to a common determinant site of neutrophils and eosinophils |
US5077216A (en) * | 1988-07-06 | 1991-12-31 | The Trustees Of Dartmouth College | Monoclonal antibodies specific for a human mononculear phagocyte-specific antigen |
US5068178A (en) * | 1988-12-06 | 1991-11-26 | Genetic Systems Corporation | Method of enhancing direct immunofluorescence staining of cells |
CA1333047C (en) * | 1989-04-14 | 1994-11-15 | Thomas Russell | Method and apparatus for obtaining at least one white blood cell population analysis |
US5006549A (en) * | 1989-05-24 | 1991-04-09 | Merck & Co., Inc. | Novel 7-aroyl-4-hydroxy-3-methyl-benzofurans as dual inhibitors of cyclooxygenase and 5-lipoxygenase |
US5061620A (en) * | 1990-03-30 | 1991-10-29 | Systemix, Inc. | Human hematopoietic stem cell |
WO1992009878A1 (en) * | 1990-11-23 | 1992-06-11 | Coulter Corporation | Method and apparatus for optically screening microscopic cells |
-
1991
- 1991-05-20 US US07/703,163 patent/US5348859A/en not_active Expired - Fee Related
-
1992
- 1992-05-11 IL IL10182692A patent/IL101826A/en active IP Right Grant
- 1992-05-11 TW TW081103640A patent/TW215955B/zh active
- 1992-05-18 AU AU20089/92A patent/AU2008992A/en not_active Abandoned
- 1992-05-18 JP JP5500244A patent/JPH06507971A/ja active Pending
- 1992-05-18 BR BR9206033A patent/BR9206033A/pt not_active Application Discontinuation
- 1992-05-18 WO PCT/US1992/004152 patent/WO1992021027A1/en not_active Application Discontinuation
- 1992-05-18 EP EP92913160A patent/EP0585385A1/en not_active Withdrawn
- 1992-05-20 CN CN92103245.5A patent/CN1067118A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
US5348859A (en) | 1994-09-20 |
TW215955B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1993-11-11 |
WO1992021027A1 (en) | 1992-11-26 |
EP0585385A1 (en) | 1994-03-09 |
AU2008992A (en) | 1992-12-30 |
CN1067118A (zh) | 1992-12-16 |
IL101826A0 (en) | 1992-12-30 |
IL101826A (en) | 1996-01-31 |
BR9206033A (pt) | 1994-11-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPH06507971A (ja) | 絶対白血球サブセット計数値および白血球の多数の部の差を得るための方法および装置 | |
AU652599B2 (en) | Method and apparatus for optically screening microscopic cells | |
Lannigan et al. | Imaging flow cytometry for the characterization of extracellular vesicles | |
US5879900A (en) | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials | |
US6682940B2 (en) | Products and methods for single parameter and multiparameter phenotyping of cells | |
CA1191079A (en) | Metachromatic dye sorption means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes | |
US4615878A (en) | Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes | |
US7537907B2 (en) | Products and methods for single parameter and multiparameter phenotyping of cells | |
US5340719A (en) | Method and apparatus for optically screening microscopic cells | |
WO2013101603A1 (en) | Cellular analysis of body fluids | |
Bocsi et al. | Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations | |
EP0866960B1 (en) | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differential | |
WO1997021994A9 (en) | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differential | |
AU775558B2 (en) | Products and methods for single parameter and multiparameter phenotyping of cells | |
JP7741825B2 (ja) | 小児腫瘍の診断、分類及び/又はモニタリングの為の手段及び方法 |