JPH06279273A - Method for removing allergen from environment and antiallergic composition - Google Patents

Abstract

PURPOSE:To obtain a method capable of stably exhibiting effects equivalent to or more than conventional technique and an antiallergic composition used for this method. CONSTITUTION:There are provided a method for removing allergen from an environment comprises treating the environment with one or more kinds of compounds selected from tea extracts, hydroxypatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid and ester compounds of gallic acid and 1-4C alcohol and the antiallergic composition used for this method.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION This invention relates to methods for removing allergens from the environment and anti-allergen compositions used in such methods.

[0002]

BACKGROUND OF THE INVENTION Diseases believed to be based on allergies such as childhood asthma, atopic dermatitis and allergic dermatitis have plagued people for many years. And to date, it has been found that antigens in indoor dust are a major cause of allergic diseases. And continually avoiding contact with allergens in these indoor dusts,
There is also much evidence to improve the symptoms (conditions) in patients with allergic symptoms, especially those with bronchial hyperreactivity.

Among allergens in the indoor dust, mites are shown as an antigen to which a large number of patients react in a patch test or the like, and in particular, the mosquito leopard mite "Dermato".
phagoides pteronyssinue ",
Acarina mites "Tyrophagus dimidi
atus "and Dermatop
"hagoides farinae". These mites (indoor dust mites) inhabit and multiply in rugs such as tatami mats, carpets and carpets, or bedding such as futons, blankets and stuffed animals, and other places where house dust accumulates. Therefore, many proposals have hitherto been made to suppress the growth of indoor dust mites by using a miticide or acaricide against the habitat of indoor dust mites. However, simply killing indoor dust mites in these places does not necessarily reduce the allergens in the environment, because dead mites are also allergenic and gradually spread allergens as they degrade Because you can. Therefore, it is clear that a drug capable of suppressing the allergenicity of allergen materials in rugs and bedding has great value.

On the other hand, the chemical nature of allergens derived from indoor dust mites is that common oxidants or reducing agents, divalent and trivalent cations, alkalis, aldehydes and mild acids reduce the allergenicity of the allergen. It has been observed to have no effect on. And other researchers have reported that the allergen has the ability to withstand even proteolytic digestion. The allergens thus prove to be very stable, which undoubtedly contributes to their survival in the environment.

As a method for reducing allergens derived from these indoor dust mites, a method that has been effective up to now has been shown to clean rugs and bedding frequently and carefully. For example, in Nishinomiya City, Hyogo Prefecture, it has been reported that the number of seizures in pediatric asthma patients is reduced by carefully cleaning the interior for about two years. In addition, as another method, Japanese Patent Laid-Open No. 61-448.
No. 21 shows a method for removing allergens from the environment and a composition therefor, characterized by treating the environment with tannic acid. However, even in these cases, the present invention is not shown.

On the other hand, examples of antiallergic agents of gallic acid derivatives include, for example, JP-A-60-199815 and JP-A-6-1988.
No. 0-199850 and JP-A No. 62-502119 show that oral administration of various gallic acid derivatives is useful for the treatment of allergies, but there is no indication of removing allergens from the environment. Not not.

[0007]

As a method for cleaning allergens in the environment with rugs and bedding frequently and carefully, for example, "Daniella allergens can be efficiently removed from the indoor environment" which is being promoted by Nishinomiya City, Hyogo Prefecture, is mentioned above. The following are the items that are routinely performed in "How to improve the environment at home to remove well".

1. Floor Cleaning The bedroom used by the patient should be vacuumed daily as much as possible. Other rooms such as the living room are preferable everyday,
Clean it about once every three days. When vacuuming the floor, it takes 20 seconds per 1 m ² (more than 30 seconds per tatami mat).

2. Dry the bedding in the sun Dry the bedding as much as possible. When taking it into the room, do it as hard as possible.

3. Clean the bedding Bedding used by the patient should be once a week
Vacuum on both the front and back (at least once every two weeks). When the patient and family sleep in the same room, vacuum the family's bedding as well. Vacuuming time is 20 seconds or more per 1 m ² .

4. Laundry of sheets and quilt covers Sheets are washed once a week. The sheets are preferably glued.

5. Bed management In the case of a two-tiered bed, vacuum the top and bottom once a week and clean the rag. Be sure to vacuum the mat used for betting once a week. The floor under the bed should also be vacuumed once a week.

Looking at the above-mentioned work menus, it can be seen that it is very difficult to carry out these menus on a daily basis instead of the usual cleaning. Therefore, an anti-allergen composition that has an allergen-removing effect equivalent to or higher than that of conventional tannic acid and that can be removed by a simple operation was examined.

[0014]

DISCLOSURE OF THE INVENTION As a result of studying a method for removing indoor allergens which is simple, easy to treat and safe, the present invention has revealed that tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin It is possible to remove allergens from the environment by treating indoors with one or more compounds selected from gallates, epigallocatechin gallates, gallic acid and ester compounds of gallic acid and alcohols having 1 to 4 carbon atoms. Found and completed the invention. That is, the present invention provides the following (a),
(B), (c), (d) and (e).

(A) It is selected from tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid and ester compounds of gallic acid with alcohols having 1 to 4 carbon atoms. A method of removing an allergen from an environment, which comprises treating the environment with one or more species.

(B) A method for removing an allergen from an environment, wherein the allergen described in (a) is an allergen derived from indoor dust mites.

(C) It is selected from tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid and ester compounds of gallic acid and alcohols having 1 to 4 carbon atoms. An anti-allergen composition containing one or more compounds.

(D) 0.1-10% by weight of tea extract,
It contains one or more compounds selected from hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid and ester compounds of gallic acid and alcohols having 1 to 4 carbon atoms. A featured anti-allergen composition.

(E) An anti-allergen composition, wherein the allergens described in (c) and (d) are allergens derived from indoor dust mites.

The allergen of the present invention is not limited as long as it causes allergenicity by human or animal skin contact or mucous membrane contact with the allergen, but it is generally of plant origin which is often contacted indoors. Allergens (pollen etc.), food-derived allergens and house dust mite-derived allergens are exemplified. In particular, as described in the prior art, allergens derived from indoor dust mites are the most important indoor allergens in which a patch test shows that 85% of asthmatic children have positive mite antigens in a certain study.

The above-mentioned indoor dust mites include mites of the subgenus Prestigmata, subgenus Astigmatism, and subgenus Astigmatism, which are Dermatophagoides farinae such as Dermatophagoides farinae and Dermatophagoides farinae, The mites such as Physcomitrella patens or the mites of Wheat may be exemplified. In particular, most of the mites present in the rugs and bedding are mosquito mites, Dermatophagoides farinae, and Dermatophagoides farinae and Plutella xylostella var. In addition to the above, as a subpredominant substigma, phytoseiid mites and dust mites such as the phytoseiid mites and dust mites, and as a substigmatid subgenus Thymis mites are also included in indoor dust mites, although in a small number.
The mites that have become attached to feather futons, which have become widespread in recent years, and the mites that enter the dwelling house, such as house dust mites, triticum mites, miraculous spiders, and saladanis, are also included in these mites.

The environment of the present invention includes a living space in a house,
Or spaces such as closets and closets where things that come into direct contact with people are stored, or walls, floors, tatami, floors such as carpets, duvets, sofas, pillows, clothes, bed pillows, curtains, floors. Examples include places that come into direct contact with people such as covers. Especially when you immediately use clothing or bedding that has been stored due to changing clothes, indoor dust mites, such as carpets on tatami mats, do not breathe easily and the temperature and humidity are stable and the humidity is about 50%.
It can be said that the place where the temperature is 25% or higher and the temperature is 25 ° C or higher is an environment that should be treated with special emphasis.

One or more selected from the group consisting of hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid and ester compounds of gallic acid and an alcohol having 1 to 4 carbon atoms of the present invention. The compound may be a single compound or a mixture of each compound. Examples of ester compounds of gallic acid and alcohols having 1 to 4 carbon atoms include methyl gallate,
Examples include ethyl gallate, propyl gallate, methylpropyl gallate and dimethylethyl gallate. And gallic acid includes not only gallic acid but also gallic acid salts such as sodium salts. Also epicatechin gallate,
Since epigallocatechin gallate is a compound in which gallic acid and catechins are ester-bonded, it is thought that ester compounds of catechins such as gallocatechin and gallic acid other than epicatechin and epigallocatechin also have similar effects. Then, a tea extract containing a large amount of catechins and ester compounds of catechins and gallic acid also has the same action, and examples of the tea extract include sun catechin (manufactured by Mitsui Norin Co., Ltd.) and polyphenone (Mitsui Norin. Company), sun oolong (manufactured by Suntory), sunflavone (manufactured by Taiyo Kagaku), and sunphenon (manufactured by Taiyo Kagaku).

The treatment method of the present invention varies depending on the formulation of the composition described below, but is not limited as long as the allergen in the environment can be treated by the active ingredient so as to lose allergenicity. For example, indoor air by spraying, spraying, applying or evaporating the composition on the floor, tatami, carpet, duvet, sofa, pillow or closet, washing clothes, or treating the filter in an air purifier. Various methods such as a method of treating

The antiallergen composition of the present invention includes tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid and gallic acid, and carbon numbers 1 to 4
One or more compounds selected from ester compounds with alcohols up to (hereinafter, referred to as "anti-allergen component") can be used as they are, but usually after being held on a solid carrier or a liquid carrier, a coating film is required if necessary. By appropriately adding a forming agent, an emulsifying agent, a fixing agent, a dispersing agent, a wetting agent, a stabilizer, a propellant, etc., an oil agent, an emulsion, a wettable powder, a spraying agent,
Aerosol agent, smoke agent, coating agent, cleaning agent, shampoo,
It can be formulated into powders and granules.

Examples of the solid carrier used for formulation herein include silicic acid, kaolin, activated carbon, bentonite,
Mineral powders such as diatomaceous earth, talc, calcium carbonate;
Examples thereof include plant powders such as wheat flour and starch; powders of synthetic polymers such as polyvinyl chloride powder; and examples of liquid carriers include water; aliphatic hydrocarbons such as hexane, kerosene, and kerosene; benzene, toluene. , Xylene and other aromatic hydrocarbons, dichloroethane, carbon tetrachloride and other halogenated hydrocarbons; ethanol, benzyl alcohol,
Alcohols such as isopropyl alcohol and ethylene glycol; ketones such as acetone, methyl ethyl ketone and cyclohexanone; ethers such as tetrahydrofuran, dimethoxyethane and diethyl ether; esters such as ethyl acetate; nitriles such as acetonitrile; acids such as dimethylformamide Amides; vegetable oils such as soybean oil, cottonseed oil and the like can be mentioned. In particular, when a gallic acid or an ester compound of gallic acid and an alcohol having 1 to 4 carbon atoms among the anti-allergen components is dissolved in a liquid carrier to prepare a formulation, a gallic acid and a liquid carrier of the ester compound are prepared. Considering solubility in ethanol, a carrier containing one or more alcohols such as ethanol, glycerin and propylene glycol is preferable. In addition, if it is a solid carrier or a liquid carrier, the liquid carrier is preferable from the viewpoint of easy treatment.

Further, as a film forming agent for holding the anti-allergen component in the environment for a long time or for adhering the anti-allergen component etc. to the solid carrier, for example, cellulose derivative, vinyl resin, alkyd resin, urea Resin, epoxy resin, polyester resin, urethane resin, silicone resin, acrylic resin, chlorinated rubber, polyvinyl alcohol, etc. are mentioned, and an emulsifier for evenly dispersing the anti-allergen component in the liquid carrier, fixation Examples of the agents and dispersants include soaps, polyoxyethylene alkyl allyl ethers, polyoxyethylene fatty acid esters, fatty acid glycerides, sorbitan fatty acid esters, sulfuric acid esters of higher alcohols, and alkyl allyl sulfonates. , Allergens Examples of propellants such as aerosol formulations in which the content is held by a liquid carrier include liquefied petroleum gas, dimethyl ether, nitrogen gas, liquefied carbon dioxide gas and pentane (including isomers such as iso-, n- and cyclo). .

When treated with an anti-allergen component to reduce environmental allergen-derived dust-borne allergen contamination, the area is treated with an insecticide (ie, acaricide) to kill live mites prior to and at the same time. Is preferred. And, it is also possible to confuse the anti-allergen component with various drugs, and as the various drugs, the conventionally used acaricide, synergist, bactericide, fungicide, deodorant and fragrance should be blended. You can also For example, as acaricides, d-phenothrin (3-phenoxybenzyl d-cis / trans-chrysanthemate), permethrin (3-phenoxybenzyl dl-cis / trans-2,2-dimethyl-3- (2 ′, 2'-dichlorovinyl) -cyclopropanecarboxylate), resmethrin ((5-benzyl-3-furyl) methyl dl-cis / trans-chrysanthemate), allethrin (dl-3-allyl-2-)
Methyl-4-oxo-2-cyclopentenyl dl-cis / trans-chrysanthemate), phthalthrin ((N-3,4,5,6, -tetrahydro-phthalimido) methyl dl-cis / trans-chrysanthemate) , Empentrin (1-ethynyl-2-methyl-2
-Pentenyl dl-cis / trans-chrysanthemate), 1-ethynyl-2-methyl-2-pentenyl-
2,2,3,3-Tetramethyl-cyclopropanecarboxylate, 1-ethynyl-2-methyl-2-pentenyl-2,2-dimethyl-3- (2 ', 2'-dichlorovinyl) -cyclopropane Carboxylate, d, dT
80-Plarethrin (d-2-methyl-4-oxo-3
-Propargyl cyclopent-2-enyl d-cis /
Trans-chrysanthemate) and tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl-3- (2, -chloro-3 ', 3', 3'-trifluoro-1-propenyl)- 2,2-dimethylcyclopropanecarboxylate), benfluthrin (2,3,3
5,6-tetrafluorobenzyl d-trans-chrysanthemate) and the like, and geometric isomers and optical isomers and derivatives thereof, and analogs thereof are used. And as the synergist and / or acaricide of the acaricide,
For example, piperonyl butoxide, octachlorodipropyl ether, N- (2-ethylhexyl) -1-isopropyl-4-methylbicyclo [2,2,2] oct-5
-Ene-2,3-dicarboximide, N- (2-ethynyl) -bicyclo [2,2,1] -hepta-5-ene-
2,3-dicarboximide or the like can be used,
In addition, other acaricides include benzyl alcohol,
Paraoxybenzoic acid ester, iodinated formal, phenols, phthalic acid ester, 3-promo-2,3-
Iodo-2-propenyl-ethyl carbonate, monoterpene ketones, monoterpene aldehydes, monoterpene epoxides, phenyl salicylate and the like can also be used.

On the other hand, as fungicides and mildew-proofing agents for suppressing the growth of mold or bacteria, which are foods for indoor dust mites,
2,4,4'-Trichloro-2'-hydroxydiphenyl ether, 2,3,5,6-tetrachloro-4-
(Methylsulfonyl) pyridine, alkylbenzylmethylammonium chloride, benzylmethyl- {2-
[2- (p-1,1,3,3-tetramethylbutylphenoxy) ethoxy] ethyl} ammonium chloride,
4-Isopropyltropolone, N, N-dimethyl-N '
-Phenyl-N '-(fluorodichloromethylthio) sulfonamide, 2- (4'-thiazolyl) benzimidazole, N- (fluorodichloromethylthio) -phthalimide, 6-acetoxy-2,4-dimethyl-m-dioxin, isopropyl Methylphenol, 0-phenylphenol, p-chloro-m-xylenol and the like are used. Further, as a deodorant for imparting indoor deodorant properties or aroma as other properties to the anti-allergen composition, lauric acid methacrylate and the like, as the fragrance, the essential oil component of rush, citronella, lemon, lemongrass, Orange, eucalyptus and lavender are also used.

The blending amount of the anti-allergen component in the anti-allergen composition of the present invention can be appropriately determined depending on the dosage form, the treatment method, the treatment location, etc., but the total amount of the anti-allergen component in the entire composition is 0.1 to 50% by weight for wettable powders and emulsions, and 0. 0 for oils and aerosols.
It is preferable to add 1 to 10% by weight. The container for the composition is preferably of a type suitable for the treatment. For example, in the case of an aerosol agent, it is easy to spray / apply it on the treatment site.
Alternatively, in the case of a fine powder, a shape capable of suppressing the scattering is preferable.

[0031]

[Action] Allergens in the environment are selected from tea extract, epicatechin, epigallocatechin, epigallocatechin gallate, epigallocatechin gallate, gallic acid and ester compounds of gallic acid and alcohols having 1 to 4 carbon atoms. It is thought that a chemical change is caused by contact with one or more kinds of compounds described above. Further, when the allergen comes into contact with hydroxyapatite, the allergen is adsorbed to the hydroxyapatite and the chance of human or animal coming into contact with the allergen is reduced, so that the allergen in the environment is apparently reduced.

[0032]

EXAMPLES The present invention will be described in detail with reference to test examples and examples, but the present invention is not limited to the examples.

Test Example 1 The antiallergenicity of various compounds was measured using mice. a) Experimental material ICR mice (6 weeks old, female) were used as experimental animals, and 4 to 18 animals were used in one test. The method for preparing a mite antigen treated with a mite antigen and an anti-allergen composition (hereinafter referred to as "drug-treated mite antigen") is as follows.

First, 3 ml of the allergen scratch extract "Torii" mite (manufactured by Torii Pharmaceutical Co., Ltd.) was placed in a molcut L ultrafiltration unit LGC (manufactured by Japan Millipore Limited) and ultrafiltered (to remove glycerin). )), And then 0.5 ml of physiological isotonic acid buffer (pH 7.5, hereinafter referred to as “PBS”) in the filter cup.
Was added to obtain a mite antigen suspension.

The drug-treated mite antigen was added to 0.2 ml of the above mite antigen suspension by adding 0.8 ml of a PBS solution containing the anti-allergen component so that the concentration of the anti-allergen component in the mixed solution was as shown in Table 1 to 25 ml. Let stand at ℃ 2 hours, then 1.
After adding 0 ml of 0.5 M phosphate buffer (pH 7.5), ultrafiltration was performed using the above ultrafiltration unit. After washing twice with 0.5 ml PBS, 1.0 ml PBS was added into the 0.5 ml filter cup to prepare.
The untreated mite antigen was prepared by treating the mite antigen suspension by allowing it to stand in a PBS solution containing no anti-allergen component at 25 ° C. for 2 hours, and thereafter performing the same procedure.

B) Test method b-1) Summary After the mice were sensitized, they were sensitized with a mite antigen and observed before induction. Then, the mite antigen solution treated with the anti-allergen component was applied to the right leg, and the untreated mite antigen solution was applied to the left leg at each site, and the observation was performed again 24 hours later.

B-2) Individual operation i) As for the sensitization treatment, I after one week of preliminary breeding was completed.
Approximately 0.2 of cyclophosamide (manufactured by SIGM) in 20 mg / ml PBS was added to each CR mouse.
5 ml was injected intraperitoneally.

Ii) As for the sensitization treatment, the mice which had been subjected to the above special number treatment were treated with a complete adjuvant (FCA) 2 days after the treatment.
(Manufactured by Difco), PBS and a mite antigen are mixed at a mixing ratio of 2: 1: 1 to prepare a mite antigen emulsification solution.
I was sensitized by injecting several spots.

Iii) As an observation before induction, mice were anaesthetized 5 days after the sensitization with ether and photographed and photographed from a distance of about 15 cm from the mouse legs to the lens in a static state as shown in FIG. After that, the thickness (2a) of the paw (1a) of the mouse in the photograph, which is indicated by the arrow, is measured with a ruler.

Iv) As the induction treatment, the anti-allergen components shown in Table 1 were applied to the right foot (1b: test foot) of the mouse before the ether anesthesia awakened after the photography in the observation before the induction. Drug-treated mite antigen, and 10 mite antigens on each mouse left foot (1c: control foot)
Induction was performed by subcutaneous injection of μl.

V) As to the observation of paw edema after the induction, as shown in FIG. 2, 24 hours after the induction treatment, a photograph was taken in the same manner as the observation before the induction, and the paw thickness of each mouse in the photograph was taken. (2b) and (2c) were measured with a ruler.

Vi) The observation result is indicated by an arrow,
The height of the edema seen above the broken lines (3b, 3c) of each foot was determined by the difference with the thickness of the foot before induction (2a), and the antiallergenicity of each drug was determined by the height of the edema of the test foot. / The height of edema of the control foot was calculated by the following formula and is shown in Table 1. Each numerical value was determined as an average value of the number of mice described in the table. The numerical value in the table indicates that the drug has no antiallergenicity, and the smaller the numerical value, the stronger the antiallergenicity.

[0043]

[Table 1]

From the above, it is considered that gallic acid, propyl gallate and hydroxyapatite have the same or higher antiallergen effect as tannic acid.

Test Example 2 The anti-allergenicity of various compounds was measured by an ELISA kit using a deer mite specific monoclonal antibody.

A) Experimental material As the mite antigen, the same mite antigen as in Test Example 1 was used, and the drug-treated mite antigen was also treated with the antiallergen components and concentrations shown in Table 2 (however, the mite-treated mite was treated. 1.0m to be added after ultrafiltration and washing in the preparation of the antigen
1.0 ml of the following D1 preparation was used instead of 1PBS). As the ELISA kit, an indoor dust mite inspection kit "DUST CHECKER" (manufactured by Shinto Paint Co., Ltd .: hereinafter referred to as "DC") was used. .

B) Test Method b-1) Preparation of Reagent and Reaction Plate i) D1 Preparation Solution: DILUENT1 20m attached to DC
1 was placed in a beaker and filled up to 200 ml with deionized water.

Ii) D3 preparation: DILUE attached to DC
Put 50ml of NT3 in a beaker and add 1 with deionized water.
It was filled up to 000 ml.

Iii) RA preparation: REAG attached to DC
0.5 ml distilled water was added to the ENT A container to dissolve the powder well, and 0.15 ml was dispensed to a microchip (manufactured by Soken Co.) containing 0.3 ml of D1 preparation liquid A 3-fold diluted solution was prepared by stirring. Similarly, dispensing stirring was repeated to prepare 9-fold dilution, 27-fold dilution and the like.

Iv) RB preparation: REAGE attached to DC
Distilled water was added to the NTB container to fill up to 12 ml.

V) Third reaction reagent: DILUE attached to DC
15 ml of NT2 was transferred to another container and kept at 35 ° C. for 10 minutes before the third reaction.
REAGENT C attached to DC and 0.015 ml of 30% hydrogen peroxide solution were added therein, and the mixture was quickly stirred to dissolve.

B-2) ELISA operation Drain the surface protection solution of the reaction plate (4) attached to DC, and
It wash | cleaned 3 times using 3 preparations. Then, as shown in FIG. 2, each dilution preparation solution of RA preparation solution is placed in wells of two columns of a1 and a2 in a well of two columns, a 1-fold dilution solution for row A, a 3-fold dilution solution for row B, and a C-solution.
9-fold dilution for row, 27-fold dilution for row D, 8 for row E
1-fold dilution, 243-fold dilution for row F, 729 for row G
A double dilution was added, and 0.1 ml of mite antigen and drug-treated mite antigen were added to the wells from A to G in the left two rows (b1, b2) of a1 and the right two rows of a2 (c1, c2), respectively. One by one, the mixture was kept at 37 ° C. for about 60 minutes with shaking (120 rpm) as the first reaction.

After the incubation was carried out with shaking, the antibody of the mite dust mite attached to the plate was sufficiently reacted with the sample, the solution in each well was discarded, and the sample was removed by washing with the D3 preparation solution about 3 times. Then, add 0.1 ml of RB preparation kept warm at 37 ° C to each well,
The temperature was kept shaking at about 60 minutes (second reaction).

After the completion of the secondary reaction in which the peroxidase-labeled Dermatophagoides farinae antibody is attached to the wells, the solution in each well is discarded again and the D3 preparation solution is washed about 3 times, and distilled water (37 ° C.) is used in the same manner. The washing was repeated about 3 times.
Then, add 0.1 ml of the third reaction reagent (substrate) to each well
The mixture was placed and kept at 37 ° C. for 30 minutes with shaking (third reaction). Then, 0.05 ml of a 2M sulfuric acid solution was added to stop the third reaction and the observation was performed. In this observation, each well was measured at a wavelength of 492 nm with a photometer for a 96-well microplate (Titer Tech Multiscan MMC / 340 Flow Laboratories), and the mite antigen residual material was measured and shown in Table 2. In the table, "-" indicates that the mite antigen residual ratio in the drug-treated mite antigen is almost zero, and "remains much".
Represented with "+".

[0055]

[Table 2]

From these, it is considered that gallic acid and propyl gallate have almost the same antiallergen effect as tannic acid.

Test Example 3 A mite antigen was applied to a cotton blanket, and various anti-allergen components were applied to the cotton blanket to reduce the allergenicity of the mite antigen adhering to the cotton blanket by using a leopard mite-specific monoclonal antibody ELISA. It was measured quantitatively with a kit.

A) Experimental material The same mite antigen as in Test Example 1 was used. DC was used as the ELISA kit. The anti-allergen component solution used in the test was a 30% ethanol solution of each anti-allergen component at each concentration shown in Table 3, and 1 w /
A phosphate buffer solution containing w% BSA (hereinafter referred to as “extraction solvent”) is 792 m of a 0.5 M phosphate buffer solution (pH 7.5).
l and 10 w /% bovine serum albumin (manufactured by Sigma) solution 8
ml was mixed.

B) Test method A cotton blanket (manufactured by Otsu Industry Co., Ltd.) was cut into 5 × 5 cm pieces (hereinafter referred to as 5
A × 5 cm cotton blanket is called a "blanket", and each blanket is sprayed with mite antigen to a volume of about 0.25 ml / sheet, and the blankets are placed in a chamber (vacuum oven) at room temperature.
LCV-230, manufactured by Tabai Co., Ltd.), and then the chamber was placed in a freeze dryer (Ochner DC-55 type, manufactured by Yamato Scientific Co., Ltd.) and a vacuum pump (mini bag, PD102).
And freeze-dried for 60 minutes.

Then, 0.06 ml of each anti-allergen component solution was sprayed onto the blanket sprayed with the mite antigen, and left at room temperature for 2 hours. Then, in order to extract the mite antigen, the blanket was immersed in 20 ml of an extraction solvent contained in a polypropylene 50 ml tube (manufactured by Corning Incorporated) and stirred 15 times by inversion stirring to extract the mite antigen of the blanket, and then the blanket was removed. Centrifuge the remaining extract at 3000 rpm for 10 minutes,
The upper layer was subjected to ultrafiltration with the same ultrafiltration unit (Morcut L) as in Test Example 1. After filtration, wash the inside of the filter cup once with 1 ml of PBS, and use the DILUE attached to the DC.
Dissolve in 0.5 ml of D1 preparation prepared from NT1,
The amount of mite antigen (A) was measured by DC.

As a comparison, nothing was sprayed on the blanket sprayed with the mite antigen, left for 2 hours and then immersed in 20 ml of the extraction solvent in the same manner as described above to extract the mite antigen, and 0.06 ml of each anti-allergen component solution. Was added. The extract was subjected to centrifugal separation treatment, and the supernatant thereof was subjected to ultrafiltration, and then the inside of the filter cup was washed once with 1 ml of PBS to prepare D1 preparation solution 0.5.
After dissolving in ml, the amount of mite antigen (B) was measured by DC. As a control, a blanket using a 23% ethanol solution instead of each anti-allergen component solution was also measured. The measurement of the amount of mite antigen on DC was performed by the same method as in Test Example 2.

C) Measurement In the same manner as in Test Example 2, each well was measured with a spectrophotometer to measure the mite antigen residual amount, and the results are shown in Table 3. In the table, the mite antigen residual rate (%) was calculated by the following formula and calculated as the average of 6 tests.

[0063]

[Table 3]

From these, it is considered that gallic acid is equivalent to tannic acid, and propyl gallate has an antiallergenic effect that is higher than tannic acid.

Example 1 Water-Soluble Agent A uniform anti-allergen composition was prepared by dissolving it in a solvent containing 30 v / v% of ethanol and 70 v / v% of water so that propyl gallate was 6 w / v%.

Example 2 Aerosol 50 v / v% ethanol, 40 v / v% No. 1 kerosene, 10 v / v% polyoxyethylene (60) hydrogenated castor oil, and 6 w / v% propyl gallate and 30 w phenyl salicylate. 200m of solution dissolved to be / v%
1 was prepared, filled in an aerosol container and fitted with a valve, and then 100 ml of liquefied petroleum gas and 5 ml of liquefied carbon dioxide gas were pressure-filled through the valve portion to prepare an anti-allergen composition.

Example 3 Water-Soluble Agent Ethanol 10 v / v%, Benzyl Alcohol 40 v /
gallic acid 6w in a solvent consisting of v% and water 50v / v%
/ V% was dissolved to prepare a uniform anti-allergen composition.

Example 4 Wettable powder 250 mesh hydroxyabatite 70 v / v
%, POE (4) lauryl ether 30 v / v% were kneaded to prepare a uniform anti-allergen composition.

Example 5 Water-soluble agent Polyphenone 60 5 w / v%, benzyl alcohol 4
A uniform anti-allergen composition was prepared by thorough mixing to give 0 v / v% and 50 v / v% water.

[0070]

INDUSTRIAL APPLICABILITY As described above, the method of removing allergen from the environment and the anti-allergen composition of the present invention use gallic acid, propyl gallate and hydroxyapatite, and thus allergens in the environment, particularly indoor dust mites. It is possible to reduce allergens derived from the family. Further, it was possible to provide a composition having a higher antiallergen effect than tannic acid, which can be treated simply and easily as compared with the conventional cleaning method, and which can be treated by the same treatment method. Furthermore, propyl gallate is a highly safe compound that can be used in cosmetics, and at the same time, it is a stable compound because it is not a natural product like tannic acid and is a synthetic compound, and an anti-allergen. It has the advantage that the effect does not vary depending on the lot.

[0071]

[Brief description of drawings]

FIG. 1 is a plan view for explaining a measuring method of Test Example 1 for explaining the present invention in detail.

FIG. 2 is a plan view for explaining each well on a reaction plate of Test Example 2 for explaining the present invention in detail.

[Explanation of symbols]

 1a Foot before induction 1b Test foot after induction 1c Control foot after induction 2a Foot height before induction 2b Height of test foot after induction 2c Height of control foot after induction 3b Prior to induction of test foot Broken line showing the height of the foot 3c Broken line showing the height of the foot before the induction of the control foot 4 Plate for ELISA

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Office reference number FI technical display location A61K 35/78 AGZ C 7822-4C

Claims (5)

[Claims] 1. A tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid, and an ester compound of gallic acid and an alcohol having 1 to 4 carbon atoms. A method of removing an allergen from an environment, which comprises treating the environment with more than one species. 2. A method for removing an allergen from an environment, wherein the allergen according to claim 1 is an allergen derived from indoor dust mites. 3. A tea extract, hydroxyapatite, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, gallic acid, and an ester compound of gallic acid and an alcohol having 1 to 4 carbon atoms. An anti-allergen composition containing one or more compounds. 4. 0.1 to 10% by weight of tea extract, hydroxyapatite, epicatechin, epigallocatechin,
An anti-allergen composition comprising one or more compounds selected from epicatechin gallate, epigallocatechin gallate, gallic acid, and ester compounds of gallic acid and an alcohol having 1 to 4 carbon atoms. 5. An anti-allergen composition, wherein the allergen according to claim 3 or 4 is an allergen derived from indoor dust mites.