WO2018092772A1 - Anti-allergenicity evaluation method and test liquid used therefor, and method for producing anti-allergenic processed product - Google Patents

Abstract

The present invention provides: a method for accurately estimating anti-allergenicity of an anti-allergenic processed product or the like for a subject; a test liquid used in said method; and a method for efficiently producing an anti-allergenic processed product, the anti-allergenicity of which has been accurately evaluated. The test liquid according to the present invention contains an allergen and water. Further, a preferred anti-allergenicity evaluation method according to the present invention comprises: bringing an aqueous solution which includes an allergen and water and in which the salt concentration of inorganic compounds is 0.04 mol/L or less into contact with a subject; then removing contaminants from recovered liquid; and taking a measurement of allergen concentration in the resultant solution.

Description

Anti-allergen evaluation method, test solution used in this method, and method for producing anti-allergen processed product

The present invention relates to an anti-allergen evaluation method performed on a subject such as an anti-allergen processed product subjected to anti-allergen processing and a test solution used in this method. The present invention also relates to a method for producing an anti-allergen processed product.

In recent years, interest in methods for preventing allergic diseases such as atopic dermatitis, bronchial asthma, and allergic rhinitis has increased. It is said that the cause of allergic diseases is inhalation of allergens that are very small and easily soar into the air. Such allergens are usually caused by ticks, pollen, mold, pet hair, etc. attached to living articles such as tatami mats, carpets, bedding, curtains, sofas, cushions, etc., trains, seats of vehicles such as automobiles, etc. . For example, it is said that leopard mites, which are dust mites that live in the house, are allergens such as worms, carcasses, shells, and dung.

The allergen is made of protein, and allergen activity can be inactivated by denaturation by heat treatment, chemical treatment with an oxidizing agent, a reducing agent, a strong acid, or a strong alkali. As another chemical treatment method, tannic acid, tea that chemically modifies the allergen molecular surface under relatively mild conditions as an anti-allergen agent that contacts allergens and denatures allergens into harmless ones. A method using an extract, a hydroxybenzoic acid compound or a salt thereof is known. It is possible to obtain an anti-allergen-processed product having anti-allergenic properties by processing such an anti-allergen agent on a substrate such as a textile product or building material by dipping, coating, spraying, kneading, or the like.

On the other hand, a method for examining whether or not an antiallergen processed product has sufficient antiallergenicity is known. For example, in the following document, a test solution in which an antiallergen-processed product is immersed in a test solution containing an allergen and allowed to react for a predetermined time, and then the test solution is collected and the antigenicity of the allergen in the test solution is measured. A method is disclosed. Japanese Patent Application Laid-Open No. 6-158494 describes a test solution in which a cedar pollen allergen is dissolved in a physiological saline solution. Japanese Patent Application Laid-Open No. 2001-212806 describes a phosphate buffer (pH 7.0) containing bovine serum albumin as a test solution. Japanese Laid-Open Patent Publication No. 2006-257376 describes a test solution in which an allergen chilled dry powder is dissolved in a phosphate buffer solution (pH 7.6) so that the amount of protein is 10 ng / mL. . Japanese Patent Application Laid-Open No. 2008-239721 describes a test solution prepared by dissolving Kona leopard mite allergen in PBS (pH 7.6) so as to be about 200 ng / mL. Japanese Patent Application Laid-Open No. 2010-116450 discloses a test solution, a standard mite allergen suspension, or a standard cedar pollen in which mite allergen is dissolved in a phosphate buffer (pH 7.2) so as to be about 450 ng / mL. A test solution containing 10 ng / mL of allergen is described. Japanese Patent Application Laid-Open No. 2010-235701 discloses a test solution prepared by dissolving a leopard mite allergen in an antigen diluent so as to be 40 ng / mL or a cedar pollen allergen dissolved in an antigen diluent so as to be 10 ng / mL. Test solutions are described.

In addition, as another evaluation method, Patent Document 7 discloses a test method for anti-allergen performance of an anti-allergen processed product in which an anti-allergen agent is contained in at least a part of the surface of a substrate, and (a) (B) a step of supplying the test liquid to the surface to be measured of the anti-allergen processed product; (b) covering the surface to be measured of the anti-allergen processed product to which the test liquid has been supplied; In a state where the test solution is spread, a step of reacting the allergen of the test solution with the anti-allergen agent on the surface to be measured of the anti-allergen processed product, and (c) collecting the test solution after the reaction and collecting allergens in the test solution A method for testing the anti-allergen performance of an anti-allergen processed product comprising the step of measuring antigenicity is disclosed. Then, mite extract freeze-dried powder was added to a phosphate buffer (pH 7.4) containing “Tween 20” (registered trademark) as a surfactant at a concentration of 0.01%, and the allergen concentration was 10 ng / ml. The test solution was described.
Further, Patent Document 8 discloses a test method for evaluating the allergen-reducing performance of a test base material processed with an anti-allergen agent, and an allergen test at a known concentration on the surface of the test base material processed with the anti-allergen agent. The allergen test solution is spread on the surface of the test substrate by placing the solution on the surface of the test substrate so that the surface of another test substrate processed with the anti-allergen agent is combined with the above-mentioned allergen test solution. The allergen reducing performance test method is characterized in that the allergen reducing performance is evaluated by analyzing the amount of allergen in the aqueous solution. And the test liquid which consists of the aqueous solution which diluted the allergen extracted from the mushroom mite with the phosphate buffer (pH 7.2) or the aqueous solution which diluted the cedar pollen allergen with the phosphate buffer (pH 7.2) is described. .

JP-A-6-158494 JP 2001-212806 A JP 2006-257376 A JP 2008-239721 A JP 2010-116450 A JP 2010-235701 A JP 2011-47778 A JP 2013-242254 A

However, in the anti-allergen test method in which the test liquid is brought into contact with the subject, the concentration of the allergen in the recovered liquid is reduced even if the base material does not contain an anti-allergen agent and is before the anti-allergen processing. In some cases, there was no difference from the allergen concentration when it was contacted. This is presumed to be due to weak adsorption of allergen on the surface of the substrate not containing the anti-allergen agent. Therefore, conventionally, a known test method can be applied only when a substrate containing no anti-allergen agent hardly adsorbs an allergen, and the anti-allergenicity cannot be accurately evaluated. Then, the subject of this invention is providing the test liquid used for the accurate evaluation method of anti-allergen property with respect to test subjects, such as a base material which is not processed with an anti-allergen, and an anti-allergen processed material, and its manufacturing raw material. is there.
Another object of the present invention is to provide a method for efficiently producing an anti-allergen processed product whose antiallergenicity has been accurately evaluated.

The present inventors have found that when an aqueous solution having a salt concentration of an inorganic compound of 0.04 mol / L or less is used in the evaluation of the antiallergenicity of a subject, the antiallergenicity can be accurately determined. The present invention is shown below.
1. A test solution used in evaluating the antiallergenicity of a subject,
A test solution containing an allergen and water.
2. 2. The test solution according to item 1, wherein the allergen concentration is 5 to 1000 ng / mL.
3. Item 3. The test solution according to Item 1 or 2, comprising an allergen and water.
4). Furthermore, the test liquid of said claim | item 1 or 2 containing a nonionic surfactant.
5). Item 5. The test solution according to Item 1, 2, or 4, further comprising bovine serum albumin.
6). Item 3. The test solution according to Item 1 or 2, comprising an allergen, water, and at least one selected from a nonionic surfactant and bovine serum albumin.
7). In a method for evaluating the antiallergenicity of a subject,
7. The test solution according to any one of items 1 to 6 or a diluted solution thereof, wherein an aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less is used.
8). Item 8. The method for evaluating antiallergenicity according to Item 7, wherein the aqueous solution is contacted with the subject, then impurities are removed from the collected liquid, and then the allergen concentration in the obtained solution is measured.
9. 9. The anti-allergenicity according to item 8, wherein when the aqueous solution is brought into contact with the subject, the contact amount of the allergen contained in the aqueous solution is 5 to 200 ng per unit area (10 cm ² ) of the subject. Evaluation methods.
10. In a method for evaluating the anti-allergen property of an anti-allergen processed product obtained by applying anti-allergen processing to a base material that has not been processed with anti-allergen,
7. The test solution according to any one of items 1 to 6 or a diluted solution thereof, wherein an aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less is brought into contact with the anti-allergen processed product, and then recovered. Removing contaminants from the resulting solution, and then measuring the allergen concentration C ₂ in the resulting solution;
The aqueous solution is contacted with the substrate, and then impurities are removed from the collected liquid, and then the allergen concentration C ₁ in the resulting solution is measured,
By using the concentration C ₂ and the concentration C _1, antiallergenic evaluation method characterized by obtaining the allergen deactivation rate.
11. When the aqueous solution is brought into contact with the anti-allergen processed product or the substrate, the contact amount of the allergen contained in the aqueous solution is 5 to 5 per unit area (10 cm ² ) of the anti-allergen processed product or the substrate. Item 10. The method for evaluating antiallergenicity according to Item 10, wherein the method is 200 ng.
12 An anti-allergen processing to a base material that has not been anti-allergen processed, and a step of confirming the anti-allergen property using the anti-allergen evaluation method according to Item 10 or 11 above. A method for manufacturing allergen processed products.

A conventionally known test solution used in the evaluation of the antiallergenicity of a subject is based on a buffer solution and is not considered to be used after diluting, and the salt concentration of the inorganic compound is at least 0. Since it was an aqueous solution having a high concentration of 1 mol / L, it was easily affected by the constituent materials of the subject regardless of the allergen concentration. In contrast, in the method for evaluating antiallergenicity of the present invention, an aqueous solution containing an allergen and having a salt concentration of an inorganic compound of 0.04 mol / L or less is less likely to be affected by the constituent materials of the subject. For example, when the subject is a base material that has not been subjected to anti-allergen processing, adsorption of the allergen to the subject can be suppressed, and anti-allergenicity evaluation can be determined more accurately. Therefore, in another anti-allergen evaluation method of the present invention, it is possible to accurately evaluate a difference in anti-allergen properties between an anti-allergen processed product and a substrate that is a raw material for producing the product, that is, an allergen inactivation effect. The aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less used in the evaluation of antiallergenicity may be the test solution of the present invention itself, and the salt concentration of the inorganic compound in the test solution of the present invention is 0.00. Even if it exceeds 04 mol / L, it may be a diluted solution with pure water or the like as long as the salt concentration of the inorganic compound is, for example, 1.0 mol / L or less.
According to the method for producing an anti-allergen processed product of the present invention, it is possible to reliably obtain an anti-allergen processed product whose anti-allergen property has been accurately evaluated.

In the present invention, the “subject” means an anti-allergen processed product containing an anti-allergen agent, a base material used in the production thereof, or an article having an anti-allergen property. The “aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less” used in the method for evaluating antiallergenicity of the present invention may be the test solution of the present invention as described above, Since it may be a liquid obtained by diluting the test liquid, in the following description, an “aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less” used in the antiallergenicity evaluation method of the present invention, It is described as “evaluation aqueous solution”.

The test liquid of the present invention is a liquid that is used as it is when evaluating the anti-allergenicity of a subject or is diluted, and an aqueous solution for evaluation is prepared, and contains allergen and water An aqueous solution having an inorganic compound salt concentration of preferably 1.0 mol / L or less, more preferably 0.1 mol / L or less, still more preferably 0.05 mol / L or less, and particularly preferably 0.04 mol / L or less. . The test solution of the present invention can be composed of an allergen and water, and can further contain other components as necessary.

The allergen is not particularly limited. In the present invention, allergens derived from pollens such as mites, cedars, cypresses, mugworts, ragweeds, hurghayas and camodia, cats, dogs, molds, cockroaches and the like. In the case of ticks, protein of mite mite or mushroom mite is often the cause of allergens. Examples of mite allergen proteins include Der f1 derived from feces of mite mites and Der f2 derived from insect mites of mites. In the case of pollen, cedar pollen allergen is mentioned, and as cedar pollen allergen protein, Cry j1 mainly present in the outer layer of cedar pollen is mentioned. Examples of allergen proteins other than the above include Bla g1 derived from cockroaches, Can f1 derived from dog dandruff, Fel d1 derived from cat dandruff, Alt a1 derived from mold, and the like.

In the present invention, the lower limit value of the concentration of allergen contained in the aqueous solution for evaluation necessary for accurate evaluation of antiallergenicity is usually 5 ng / mL. Therefore, in the evaluation, if necessary, it may be diluted with pure water or the like. Therefore, even if the test solution of the present invention is a test solution containing a high concentration of allergen, it should have storage stability. Can be used without any problems. The concentration of the allergen contained in the test solution of the present invention is not particularly limited, but from the viewpoint of the stability of the test solution, it is preferably 5 to 1000 ng / mL, more preferably 8 to 200 ng / mL, still more preferably 10 to 100 ng / mL. When a test solution having an allergen concentration lower than 5 ng / mL is used as an aqueous solution for evaluation, there is a tendency that the performance difference when an anti-allergen processed product and a base material that is a manufacturing raw material are evaluated is reduced. .

The test solution of the present invention is preferably water-soluble and does not react with allergens when it contains other components. Examples of other components include surfactant, bovine serum albumin (hereinafter referred to as “BSA”), casein, gelatin, sugar, phosphate, thiosulfate, sodium chloride, and the like. The test solution of the present invention may contain a water-soluble inorganic compound such as the above exemplary compounds, but is diluted with pure water or the like, and the aqueous solution for evaluation having an inorganic compound salt concentration of 0.04 mol / L or less. In view of facilitating preparation of the inorganic compound, the upper limit of the salt concentration of the inorganic compound is preferably 1.0 mol / L, more preferably 0.1 mol / L, and even more preferably 0.08 mol / L. Conventionally, in the case of a test solution containing a known buffer solution and allergen, in order to prepare an aqueous solution for evaluation used for evaluating the anti-allergenicity of a subject, the dilution rate of the test solution must be extremely high. Therefore, there is a possibility that the inaccuracy of the allergen concentration cannot be obtained by the evaluation aqueous solution after dilution.

The surfactant is preferably a nonionic surfactant. As this nonionic surfactant, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene fatty acid amide, polyoxyethylene alkylamine, sorbitan fatty acid ester, etc. Is mentioned. Among these, for example, when evaluating the anti-allergen property of the substrate before anti-allergen processing, the allergen adsorption on the surface is suppressed, and the anti-allergen property in the anti-allergen processed product is more accurately evaluated. Polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan trilaurate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan tristearate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan trioleate More preferred are sorbitan fatty acid esters such as ate, and particularly preferred is polyoxyethylene sorbitan monolaurate.

When the test solution of the present invention contains a surfactant, the concentration thereof is preferably 0.001 to 5.0% by mass with respect to the entire test solution.

BSA is a bovine serum derived product albumin (Bovine serum albumin), a protein of about 66.000 Da. This BSA has the same action as the nonionic surfactant.
When the test solution of the present invention contains BSA, the concentration thereof is preferably 0.0005 to 5.0% by mass with respect to the entire test solution.

Examples of the phosphate include sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, and the like.
Examples of the thiosulfate include sodium thiosulfate, potassium thiosulfate, and ammonium thiosulfate.

In the present invention, preferred test solutions are exemplified below.
(S-1) A liquid comprising an allergen and water.
(S-2) A liquid containing an allergen, an inorganic compound and water.
(S-3) A liquid comprising an allergen, a nonionic surfactant and water.
(S-4) A liquid containing an allergen, a nonionic surfactant, an inorganic compound, and water.
(S-5) A liquid comprising an allergen, bovine serum albumin and water.
(S-6) A liquid containing allergen, bovine serum albumin and water.
(S-7) A liquid comprising an allergen, a nonionic surfactant, bovine serum albumin and water.
(S-8) A liquid containing an allergen, a nonionic surfactant, bovine serum albumin, an inorganic compound, and water.
The liquids (S-1) to (S-8) may be liquids containing allergens exceeding the upper limit of the preferable concentration. The liquids (S-2), (S-4), (S-6), and (S-8) are particularly preferably liquids having a salt concentration of an inorganic compound of 0.04 mol / L or less. In another embodiment, the liquid is preferably a liquid that can reduce the salt concentration of the inorganic compound to 0.04 mol / L or less by diluting 2 to 100 times in terms of volume with water.

The test solution of the present invention can be produced by dissolving the allergen and other components used as necessary in water (pure water or the like). Moreover, after dissolving each component separately in water, it can also manufacture by mixing the obtained aqueous solution.

The method for evaluating the antiallergenicity of a subject in the present invention is the test solution of the present invention or a diluted solution thereof, wherein an aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less, that is, an evaluation aqueous solution. It is characterized by using.

The salt concentration of the inorganic compound in the aqueous solution for evaluation is 0.04 mol / L or less, preferably 0.01 mol / L or less, more preferably 0.001 mol / L or less. When the salt concentration is 0.04 mol / L or less, for example, when a base material before anti-allergen processing is used as a subject and the anti-allergen property is evaluated, an anti-allergenicity obtained using this base material is obtained. Since the difference becomes clear when compared with the case where the antiallergen property is evaluated using the allergen processed product as a subject, the performance of the antiallergen processed product can be more accurately evaluated. The inorganic compound that forms a salt when the salt concentration in the aqueous solution for evaluation is not 0 mol / L is not particularly limited, but is preferably a phosphate.

The evaluation aqueous solution may contain a surfactant, BSA, and the like. When the surfactant is contained, the concentration thereof is preferably 0.001 to 0.40% by mass with respect to the entire evaluation aqueous solution. More preferably, the content is 0.005 to 0.30% by mass, and still more preferably 0.01 to 0.20% by mass. When BSA is contained, the concentration thereof is preferably 0.0005 to 0.60% by mass, more preferably 0.001 to 0.50% by mass, still more preferably 0.005% by mass, based on the entire aqueous solution for evaluation. 01 to 0.03 mass%.

In the present invention, preferable aqueous solutions for evaluation are exemplified below.
(L-1) An aqueous solution comprising an allergen and water.
(L-2) An aqueous solution containing an allergen, an inorganic compound, and water.
(L-3) An aqueous solution comprising an allergen, a nonionic surfactant and water.
(L-4) An aqueous solution containing an allergen, a nonionic surfactant, an inorganic compound, and water.
(L-5) An aqueous solution comprising allergen, bovine serum albumin and water.
(L-6) An aqueous solution containing allergen, bovine serum albumin and water.
(L-7) An aqueous solution comprising an allergen, a nonionic surfactant, bovine serum albumin and water.
(L-8) An aqueous solution containing an allergen, a nonionic surfactant, bovine serum albumin, an inorganic compound, and water.

Examples of the subject include fibers or fiber products, wood products, paper products, plastic products, rubber products, glass products, ceramic products, stone products, and the like. The base material may be a composite composed of two or more materials.

The shape of the subject can be a linear shape, a sheet shape, a plate shape, a lump shape, or the like, and is not particularly limited. The size of the subject is not particularly limited, but a surface area of about 5 to 200 cm ² is appropriate. Usually, the entire article or the surface of a specific portion is evaluated as a square such as a square or a rectangle. It is good also as a circle.

In the method for evaluating anti-allergenicity of a subject in the present invention, preferably, the aqueous solution for evaluation is brought into contact with the subject, and then impurities are removed from the collected liquid, and then the obtained solution (hereinafter, “ It is a method for measuring the allergen concentration in the “recovered solution”.

The amount of the aqueous solution for evaluation is appropriately selected depending on the shape and size of the subject. When the subject does not absorb water, the amount of the aqueous solution for evaluation correlates with the surface area. The larger the surface area, the more the amount of aqueous solution for evaluation needs to be increased. In the present invention, the amount of allergen contact per unit area (10 cm ² ) of the subject is important in order to reliably determine the anti-allergen performance. Therefore, when an evaluation aqueous solution with a known allergen concentration and a subject with a known shape and size are used, the amount of allergen that contacts the subject is preferably 5 per unit area (10 cm ² ) of the subject. The aqueous solution for evaluation is used so that the amount becomes ˜200 ng, more preferably 10 to 150 ng, still more preferably 20 to 100 ng.

When contacting the aqueous solution for evaluation with the subject, a method of placing the aqueous solution for evaluation on the surface of the subject, a method of immersing the subject in the aqueous solution for evaluation, etc. are applied, and the contact efficiency between the aqueous solution for evaluation and the subject It can be combined with a device to improve.
As a method for increasing the contact efficiency between the aqueous solution for evaluation and the subject, for example, the evaluation aqueous solution and the subject are accommodated in a container such as a plastic bag that can just accommodate the subject, and the air in the container is evacuated as much as possible. The method of sealing in an immersion state is mentioned. For example, when a subject has a plane part, a test solution may be sandwiched between two subjects using each plane part. Furthermore, it can also be set as the method of dropping the aqueous solution for evaluation on the surface of a subject, and then covering the film, and interposing the aqueous solution for evaluation between the film and the subject.

The contact temperature of the aqueous solution for evaluation and the subject is not particularly limited. This temperature is preferably constant in the range of 4 ° C. to 37 ° C., more preferably 5 ° C. to 25 ° C., from the viewpoint of the reactivity of the allergen contained in the test solution. In order to keep the temperature constant, the aqueous solution for evaluation and the subject can be contacted in a thermostatic chamber.

The contact time between the aqueous solution for evaluation and the subject is appropriately selected depending on the size of the subject and the like, but is preferably in the range of 5 minutes to 48 hours, preferably in the range of 30 minutes to 24 hours, and more preferably. It ranges from 1 hour to 6 hours. When the contact time is set to be long, the evaluation aqueous solution and the subject that are in contact with each other are stored in a sealable container in order to prevent the evaluation aqueous solution from decreasing due to volatilization or evaporation and changing the allergen concentration. It is preferable to keep it. Such a container is not particularly limited, and examples thereof include a glass chamber, a glass desiccator, and a polyethylene bag with a chuck. Furthermore, when the internal volume of the container is relatively large while the amount of the aqueous solution for evaluation used is small, a decrease due to volatilization of the aqueous solution for evaluation in the container may affect the test. In order to suppress this, it is preferable to adjust the humidity in the container. For example, absorbent cotton containing water or a container containing water can be accommodated.

After contacting the above aqueous solution for evaluation and the subject for a certain time, the aqueous solution for evaluation is collected. The recovery method is not particularly limited, but the test is performed with a solution that does not denature the allergen, such as a method of recovering the aqueous solution for evaluation with a pipette, the same medium (liquid that does not contain allergen) as the aqueous solution for evaluation used, or a phosphate buffer solution. Examples thereof include a method of washing the body, a method of absorbing the aqueous solution for evaluation by a non-woven fabric and the like, and collecting the solution. Depending on the type of subject, the aqueous test solution may be absorbed by the subject and difficult to recover. When the subject has flexibility, it is possible to compress the subject and squeeze out the aqueous solution for evaluation. Thereafter, contaminants are removed from the collected liquid using centrifugation or the like. Then, the obtained recovered solution may be used for analysis as it is, or the recovered solution may be concentrated before analysis in order to improve measurement sensitivity. The method for concentrating the recovered solution is not particularly limited, but a method that is difficult to denature the allergen that maintains the reactivity to the specific antibody present in the recovered solution is preferable, and such a method is performed by filtering through an ultrafiltration membrane. And a lyophilization method.

Thereafter, the concentration of the remaining allergen is measured by analyzing the allergen antigenicity in the collected solution. The amount of allergen antigen using the antigen-antibody reaction corresponds to the amount of residual allergen. When analyzing the antigenicity of an allergen that maintains the reactivity to a specific antibody present in a collected solution, for example, an enzyme immunoassay method using an ELISA kit (Indoor Biotechnologies, Nitinichi Pharmaceutical, ITEA, etc.) Alternatively, a method using an allergen measuring instrument such as “Mighty Checker” manufactured by Shinto Fine Co., Ltd. or “Daniscan” manufactured by Asahi Breweries, Inc. can be used.

By the above method, regardless of the type of the subject, the antiallergenicity of the subject can be determined from the concentration of the remaining allergen. When a plurality of subjects are evaluated, a subject with a low residual allergen concentration is determined to have high anti-allergen properties, and a subject with a high residual allergen concentration is determined to have low anti-allergen properties.
When the subject is a base material that has not been anti-allergen-processed and an anti-allergen-processed product manufactured using this base material, the collected solution after contacting each with an aqueous solution for evaluation is used. By analyzing the concentration of the allergen contained (residual allergen), the antiallergenicity imparted to the substrate can be accurately grasped. That is, in another anti-allergen evaluation method of the present invention, an aqueous solution for evaluation containing an allergen at a concentration C ₀ is brought into contact with an anti-allergen processed product, and then impurities are removed from the collected liquid, and then obtained. solution was measured allergen concentrations C ₂ in the (recovered solution), in a similar manner, separately, the same evaluation solution, contacting the substrate that are not anti-allergen processed, after which the contaminants from the recovered liquid Next, the allergen concentration C ₁ in the obtained solution (recovered solution) is measured, and the difference between the obtained concentration C ₂ and concentration C ₁ is calculated. Since each allergen concentration can be obtained by the above method, the allergen inactivation rate of the anti-allergen processed product can be calculated by the following formula.
Allergen inactivation rate (%) = [(C ₁ -C ₂ ) / C ₀ )] × 100

In the present invention, an anti-allergen processed product in which anti-allergen properties are accurately evaluated can be produced using the above-described other anti-allergen property evaluation methods of the present invention. That is, the method for producing an anti-allergen processed product of the present invention comprises a step of applying anti-allergen processing to a substrate that has not been anti-allergen processed (hereinafter referred to as “first step”), and the other anti-allergens of the present invention. A step of confirming antiallergenicity using a sex evaluation method (hereinafter referred to as “second step”).

In the first step, not only an aspect in which an anti-allergen processing is applied to a substrate having the same or the same form as the anti-allergen processed product of the final product, but also an anti-allergen processed fiber such as a nonwoven fabric It includes an embodiment in which a woven fabric, a nonwoven fabric, or the like is formed. The substrate is not particularly limited, and fibers, woven fabrics, nonwoven fabrics, knitted fabrics, wood products, paper products, plastic products, rubber products, glass products, ceramic products, stone products, and the like can be used. The base material may be a composite composed of two or more materials.

The anti-allergen agent used in the anti-allergen processing may be either an organic material or an inorganic material, or a combination thereof. Organic materials include tannic acid, gallotannin, epicatechin gallate, epicatechin, epigallocatechin, epigallocatechin gallate, natural polyphenols such as persimmon astringent, polymers of paravinylphenol which is a synthetic polyphenol, dihydroxybenzoic acid, 2 , 4,6-trihydroxybenzoic acid and other hydroxybenzoic acid compounds or salts thereof, polystyrene compounds such as polystyrene sulfonate, and extracts such as olive leaves and ginkgo biloba. Furthermore, as inorganic materials, zinc oxide which is a metal oxide, titanium oxide and tungsten oxides having a photocatalytic function, silicon dioxide, aluminum oxide, zirconium oxide, zirconium hydroxide, zirconium phosphate, aluminum phosphate, tin phosphate , Cerium phosphate, titanium phosphate, H-substituted Y-type zeolite, H-substituted ZSM-5-type zeolite, antimonic acid, SiO ₂ —Al ₂ O ₃ composite oxide, SiO ₂ —TiO ₂ composite oxide, SiO ₂ —ZrO Composite oxide, SiO ₂ —Ga ₂ O ₃ composite oxide, TiO ₂ —Al ₂ O ₃ composite oxide, TiO ₂ —ZrO composite oxide, TiO ₂ —SnO composite oxide, TiO ₂ —ZnO composite oxide, Magnesium silicate or water-soluble inorganic salt calcium silicate, aluminum silicate, strontium silicate, disilicate Koniumu and inorganic salt-based compounds such as silicates of rare earth elements and the like.

The antiallergen is usually liquid or solid. Therefore, in the first step, when the anti-allergen agent is bound to the base material, a solution obtained by dissolving the active ingredient of the anti-allergen agent in water or a solvent, or a dispersant if not dissolved in the medium An anti-allergen-processed base material can be obtained by applying a processing liquid containing a combination of the above and a processing liquid containing a binder to the base material and drying. The coating method can be appropriately selected depending on the shape of the substrate, and can be dip coating, spray coating, brush coating, curtain coating, spin coating, or the like.
The concentration of the anti-allergen agent in the processing liquid is preferably 5 to 50% by mass, more preferably 10 to 40% by mass. The binding amount of the anti-allergen agent to the base material is usually 0.5 to 3.0 g per unit area (1 m ² ).

Then, in a 2nd process, anti-allergenicity is confirmed about the base material and an anti-allergen processed product using the said other anti-allergenic evaluation method of this invention. Thus, with the allergen concentrations C ₁ and C _2, it is possible to calculate the allergen deactivation rate, it is possible to accurately grasp the anti-allergenic anti-allergen processed products with anti-allergen agent used.

Hereinafter, the present invention will be described in more detail with reference to experimental examples.

1. Base materials and anti-allergen processed products The subjects subjected to the evaluation of anti-allergens were the following base materials (fiber sheets and wallpaper base paper that were not anti-allergen processed) and anti-allergen processed products (anti-allergen sheets and anti-allergens) Sex wallpaper).

1-1. Fiber sheet and anti-allergenic sheet The fiber sheet is a woven fabric obtained using polyester fibers, and has a size of 40 mm × 50 mm and a basis weight of 180 g / m ² .
Further, the anti-allergenic sheet is obtained by immersing the above fiber sheet in a processing liquid containing an anti-allergen agent “Alleluve ZS200” (trade name) manufactured by Toagosei Co., Ltd. and an acrylic binder, and then drying. The adhesion amount of the anti-allergen agent is 0.75 g / m ² .

1-2. Wallpaper base paper and anti-allergenic wallpaper Wallpaper base paper is a commercially available plain white wallpaper having a size of 40 mm × 50 mm.
In addition, the anti-allergenic wallpaper is obtained by applying and drying the above-mentioned base paper on the surface of one side of the processing liquid containing the anti-allergen agent “Alleluve ZTP170” (trade name) manufactured by Toagosei Co., Ltd. and an acrylic binder. In this case, the adhesion amount of the anti-allergen is 3.0 g / m ² .

2. Anti-allergen evaluation method As an evaluation method of anti-allergen properties in subjects (base materials and anti-allergen processed products), Japanese allergen allergen (generally called “Der f2”) and cedar pollen allergen (generally, allergen) The sandwich method in the ELISA method using an allergen called “Cry j1” was applied. In the following experimental examples, the leopard mite allergen (hereinafter simply referred to as “mite allergen”) was used. The test operation is as follows.
In the manner described in each experimental example, the evaluation aqueous solution (20 ° C.) shown in Tables 1 to 3 is brought into contact with a subject (base material and anti-allergen processed product) of a predetermined size (40 mm × 50 mm) and centrifuged. The supernatant was recovered. Then, a specific antibody “15E11 antibody” (manufactured by Asahi Breweries) treated with a blocking agent was added to the supernatant of the antibody-coated well prepared by a conventional method. Subsequently, it was left to stand at about 25 ° C., and after 1 hour, the test solution was discarded and each well was washed with a washing buffer. Then, “horseradish peroxidase-labeled anti-Der f2 monoclonal antibody 13A4PO” (manufactured by Asahi Breweries) diluted to 200 ng / mL with a washing buffer was added to each well and allowed to stand at about 25 ° C. After 1 hour, the antibody solution was discarded, each well was washed with a washing buffer, a substrate solution was added to each well, and the mixture was allowed to stand at about 25 ° C. Thereafter, the reaction was stopped by adding an aqueous 2N-sulfuric acid solution, the absorbance at 490 nm was measured, and a residual mite allergen concentration was obtained using a calibration curve. This calibration curve is for mite allergen aqueous solutions prepared using mite allergens and pure water, with mite allergen concentrations adjusted to 0 ng / mL, 5 ng / mL, 10 ng / mL, 20 ng / mL and 40 ng / mL, respectively. 5 is a straight line showing the relationship between the absorbance and the mite allergen concentration obtained by applying the ELISA method. When evaluating the anti-allergen properties of the anti-allergen processed product, the residual mite allergen concentration C ₂ in the anti-allergen processed product and the residual mite allergen concentration C _{1 in the} base material obtained by the ELISA method using each aqueous solution for evaluation. Was used to calculate the allergen inactivation rate (%) of the anti-allergen processed product from the following formula. The higher the allergen inactivation rate, the better the antiallergenicity.
Allergen inactivation rate (%) = [(C ₁ -C ₂ ) / C ₀ )] × 100
(C ₀ is the concentration of allergen in the aqueous solution for evaluation)

3. Production of test solution Preparation Example 1
The mite allergen 50 μg was dissolved in pure water 7.5 mL to obtain a mite allergen aqueous solution having a mite allergen concentration of 10000 ng / 1.5 mL. Hereinafter, this is referred to as “test solution (L1)”.

Preparation Example 2
10 μg of the mite allergen was dissolved in 15 mL of pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 10000 ng / 15 mL. Thereafter, polyoxyethylene (20) sorbitan monolaurate “Tween 20” (registered trademark) manufactured by Wako Pure Chemical Industries, Ltd., which is a nonionic surfactant, is added to this aqueous solution so that the concentration becomes 7.5% by mass. To obtain an aqueous solution having a mite allergen concentration of 10,000 ng / 15 mL and a surfactant concentration of 7.5% by mass. Hereinafter, this is referred to as “test solution (L2)”.

Preparation Example 3
The mite allergen 20 μg was dissolved in 15 mL of pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 20000 ng / 15 mL. Thereafter, BSA manufactured by SIGMA was added to this aqueous solution so as to have a concentration of 5% by mass, and an aqueous solution having a mite allergen concentration of 20000 ng / 15 mL and a BSA concentration of 5% by mass was obtained. Hereinafter, this is referred to as “test solution (L3)”.

Preparation Example 4
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 20 ng / 1.5 mL. Thereafter, “Tween 20” (registered trademark) and BSA are added to the diluted solution so as to have concentrations of 0.15% by mass and 0.1% by mass, respectively, and the test solution (L4) is added. Obtained. The mite allergen contained in this test solution (L4) is 20 ng / 1.5 mL, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass.

Preparation Example 5
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, “Tween 20” (registered trademark) and BSA were added to the diluted solution so as to have concentrations of 0.15% by mass and 0.1% by mass, respectively, and dissolved, and the test solution (L5) was added. Obtained. The mite allergen contained in this test solution (L5) is 40 ng / 1.5 mL, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass.

Preparation Example 6
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 100 ng / 1.5 mL. Thereafter, “Tween 20” (registered trademark) and BSA were added to the diluted solution so as to have concentrations of 0.15% by mass and 0.1% by mass, respectively, and the test solution (L6) was dissolved. Obtained. The mite allergen contained in this test liquid (L6) is 100 ng / 1.5 mL, the surfactant concentration is 0.15 mass%, and the BSA concentration is 0.1 mass%.

Preparation Example 7
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, to this diluted solution, disodium hydrogen phosphate 12 hydrate, sodium dihydrogen phosphate dihydrate, “Tween 20” (registered trademark) and BSA were added at concentrations of 0.0005 mol / L, 0, respectively. .0005 mol / L, 0.15% by mass and 0.1% by mass were added and dissolved to obtain a test solution (L7). The mite allergen contained in this test solution (L7) is 40 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.001 mol / L, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass. is there.

Preparation Example 8
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, “Tween 20” (registered trademark) and BSA were added to this diluted solution at concentrations of 0.01 mol / L, 0 The test solution (L8) was obtained by adding and dissolving to 0.01 mol / L, 0.15 mass%, and 0.1 mass%. The mite allergen contained in this test solution (L8) is 40 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.02 mol / L, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass. is there.

Preparation Example 9
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, “Tween 20” (registered trademark) and BSA are added to the diluted solution so as to have concentrations of 0.15% by mass and 0.001% by mass, respectively, and the test solution (L9) is added. Obtained. The mite allergen contained in this test solution (L9) is 40 ng / 1.5 mL, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.001% by mass.

Preparation Example 10
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, “Tween20” (registered trademark) and BSA are added to the diluted solution so as to have concentrations of 0.015% by mass and 0.05% by mass, respectively, and the test solution (L10) is added. Obtained. The mite allergen contained in this test solution (L10) is 40 ng / 1.5 mL, the surfactant concentration is 0.015 mass%, and the BSA concentration is 0.05 mass%.

Preparation Example 11
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, disodium hydrogenphosphate dodecahydrate, sodium dihydrogenphosphate dihydrate and BSA were added to this diluted solution at concentrations of 0.005 mol / L, 0.005 mol / L and 0.01 respectively. It added and dissolved so that it might become mass%, and the test liquid (L11) was obtained. The mite allergen contained in this test solution (L11) is 40 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.01 mol / L, and the BSA concentration is 0.01% by mass.

Preparation Example 12
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 5 ng / 1.5 mL. Thereafter, disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, “Tween 20” (registered trademark) and BSA were added to this diluted solution at concentrations of 0.025 mol / L, 0 0.025 mol / L, 0.15% by mass, and 0.1% by mass were added and dissolved to obtain a test solution (L12). The mite allergen contained in this test solution (L12) is 5 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.05 mol / L, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass. is there.

Preparation Example 13
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 9 ng / 5.0 mL. Thereafter, disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, “Tween 20” (registered trademark) and BSA were added to this diluted solution at concentrations of 0.025 mol / L, 0 0.025 mol / L, 0.15% by mass and 0.1% by mass were added and dissolved to obtain a test solution (L13). The mite allergen contained in this test solution (L13) is 9 ng / 5.0 mL, the salt concentration of the inorganic compound is 0.05 mol / L, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass. is there.

Preparation Example 14
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, to this diluted solution, disodium hydrogen phosphate 12 hydrate, sodium dihydrogen phosphate dihydrate, “Tween 20” (registered trademark) and BSA were added at concentrations of 0.05 mol / L, 0 .05 mol / L, 0.15% by mass and 0.1% by mass were added and dissolved to obtain a test liquid (L14). The mite allergen contained in this test liquid (L14) is 40 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.1 mol / L, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass. is there.

Preparation Example 15
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 300 ng / 1.5 mL. Thereafter, to this diluted solution, disodium hydrogen phosphate 12 hydrate, sodium dihydrogen phosphate dihydrate, “Tween 20” (registered trademark) and BSA were added at concentrations of 0.05 mol / L, 0 .05 mol / L, 0.15% by mass and 0.1% by mass were added and dissolved to obtain a test solution (L15). The mite allergen contained in this test solution (L15) is 300 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.1 mol / L, the surfactant concentration is 0.15% by mass, and the BSA concentration is 0.1% by mass. is there.

Preparation Example 16
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, “Tween 20” (registered trademark) and BSA are added to this diluted solution so as to have concentrations of 0.1% by mass and 0.1% by mass, respectively, and dissolved, and the test solution (L16) is added. Obtained. The mite allergen contained in this test solution (L16) is 40 ng / 1.5 mL, the surfactant concentration is 0.1 mass%, and the BSA concentration is 0.1 mass%.

Preparation Example 17
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, disodium hydrogen phosphate dodecahydrate and sodium dihydrogen phosphate dihydrate were added to the diluted solution so that the concentrations were 0.3 mol / L and 0.3 mol / L, respectively. To obtain a test liquid (L17). The mite allergen contained in this test solution (L17) is 40 ng / 1.5 mL, and the salt concentration of the inorganic compound is 0.6 mol / L.

Preparation Example 18
The mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain a mite allergen aqueous solution having a mite allergen concentration of 40 ng / 1.5 mL. Thereafter, to this diluted solution, disodium hydrogen phosphate 12 hydrate, sodium dihydrogen phosphate dihydrate and “Tween 20” (registered trademark) were added at concentrations of 0.3 mol / L and 0.3 mol, respectively. / L and 0.1% by mass were added and dissolved to obtain a test liquid (L18). The mite allergen contained in this test solution (L18) is 40 ng / 1.5 mL, the salt concentration of the inorganic compound is 0.6 mol / L, and the surfactant concentration is 0.1% by mass.

4). Antiallergenicity Evaluation of Antiallergenic Sheet Example 1-1
The test solution (L1) composed of the mite allergen aqueous solution having a mite allergen concentration of 10000 ng / 1.5 mL obtained in Preparation Example 1 was diluted 25 times with pure water, and an aqueous solution for evaluation having a mite allergen concentration of 400 ng / 15 mL was prepared. Obtained.
Next, 1.5 mL of this aqueous solution for evaluation was previously placed in each polyethylene bag in which a subject of a predetermined size, that is, a base material (fiber sheet) and an antiallergenic sheet (processed sheet) were separately accommodated. Each test subject was sufficiently infiltrated with the aqueous solution for evaluation by putting and lightly pressing. The contact amount of the mite allergen to the subject is 20 ng per 10 cm ² (see Table 1). Then, the test subject was stored in a sealed state at 20 ° C. for 3 hours. Thereafter, about 0.5 mL of the aqueous solution for evaluation was collected and centrifuged. Next, the obtained supernatant was applied to the above method to determine the residual mite allergen concentration per volume of the aqueous solution for evaluation used, and the allergen inactivation rate was measured. The results are shown in Table 1.

Example 1-2
The test solution (L2) obtained in Preparation Example 2 was diluted 50 times with pure water to obtain an aqueous solution for evaluation having a mite allergen concentration of 200 ng / 15 mL and a surfactant concentration of 0.15% by mass.
Next, the same operation as in Example 1-1 was performed except that 3.0 mL of this aqueous solution for evaluation was brought into contact with the subject, and an allergen inactivation rate was obtained (see Table 1).

Example 1-3
The test solution (L3) obtained in Preparation Example 3 was diluted 50 times with pure water to obtain an aqueous solution for evaluation having a mite allergen concentration of 400 ng / 15 mL and a BSA concentration of 0.1% by mass.
Subsequently, the same operation as in Example 1-1 was performed except that 1.5 mL of the aqueous solution for evaluation was brought into contact with the subject for 24 hours, and an allergen inactivation rate was obtained (see Table 1).

Example 1-4
The test solution (L4) obtained in Preparation Example 4 was used as an aqueous solution for evaluation, and the same procedure as in Example 1-1 was performed except that 1.5 mL of the test solution was contacted with the subject. Obtained (see Table 1).

Example 1-5
The test solution (L4) obtained in Preparation Example 4 was used as an aqueous solution for evaluation, and the same operation as in Example 1-1 was performed except that 3.0 mL of the test solution was brought into contact with the subject. Obtained (see Table 1).

Example 1-6
The test solution (L5) obtained in Preparation Example 5 was used as an aqueous solution for evaluation, and the same procedure as in Example 1-1 was performed except that 1.5 mL of the test solution was contacted with the subject. Obtained (see Table 1).

Example 1-7
The test solution (L6) obtained in Preparation Example 6 was used as an aqueous solution for evaluation, and the same procedure as in Example 1-1 was performed except that 1.5 mL of the test solution was contacted with the subject. Obtained (see Table 1).

Example 1-8
The test solution (L7) obtained in Preparation Example 7 was used as an aqueous solution for evaluation, and the same operation as in Example 1-1 was performed except that 1.5 mL of the test solution was brought into contact with the subject. Obtained (see Table 1).

Example 1-9
The test solution (L8) obtained in Preparation Example 8 was used as an aqueous solution for evaluation, and the same procedure as in Example 1-1 was performed except that 1.5 mL of the test solution was brought into contact with the subject. Obtained (see Table 1).

Example 1-10
Allergen inactivation was carried out in the same manner as in Example 1-1 except that the test solution (L9) obtained in Preparation Example 9 was used as an aqueous solution for evaluation, and 1.5 mL thereof was contacted with the subject for 1 hour. The rate was obtained (see Table 1).

Example 1-11
The same procedure as in Example 1-1 was performed, except that the test solution (L10) obtained in Preparation Example 10 was used as an aqueous solution for evaluation and 1.5 mL thereof was contacted with the subject at 5 ° C. for 1 hour. An inactivation rate was obtained (see Table 1).

Example 1-12
Allergen inactivation was carried out in the same manner as in Example 1-1 except that the test solution (L11) obtained in Preparation Example 11 was used as an aqueous solution for evaluation, and 1.5 mL thereof was contacted with the subject for 1 hour. The rate was obtained (see Table 1).

Comparative Example 1-1
The test solution (L12) obtained in Preparation Example 12 was used as an aqueous solution for evaluation, and the same operation as in Example 1-1 was performed except that 1.5 mL of the test solution was brought into contact with the subject. Obtained (see Table 2).

Comparative Example 1-2
The test solution (L13) obtained in Preparation Example 13 was used as an aqueous solution for evaluation, and the same operation as in Example 1-1 was performed except that 5.0 mL of the test solution was brought into contact with the subject. Obtained (see Table 2).

Comparative Example 1-3
The test solution (L14) obtained in Preparation Example 14 was used as an aqueous solution for evaluation, and the same operation as in Example 1-1 was performed except that 1.5 mL thereof was brought into contact with the subject. Obtained (see Table 2).

Comparative Example 1-4
Allergen inactivation was carried out in the same manner as in Example 1-1 except that the test solution (L15) obtained in Preparation Example 15 was used as an aqueous solution for evaluation, and 1.5 mL thereof was contacted with the subject for 24 hours. The rate was obtained (see Table 2).

According to Table 1 and Table 2, when the antiallergenicity of a subject is evaluated, the aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less is brought into contact with the subject as an aqueous solution for evaluation. It is clear that it is not easily affected by the constituent materials. Moreover, anti-allergenicity could be more accurately evaluated by using a nonionic surfactant and BSA together.

5). Antiallergenicity Evaluation of Antiallergenic Wallpaper Example 2-1
The test liquid (L1) consisting of a mite allergen aqueous solution having a mite allergen concentration of 10,000 ng / 1.5 mL obtained in Preparation Example 1 was diluted with pure water to obtain an aqueous solution for evaluation having a mite allergen concentration of 400 ng / 15 mL.
Next, 1.5 mL of this aqueous solution for evaluation was previously placed in each polyethylene bag that separately contained a subject of a predetermined size, that is, a base material (wallpaper base paper) and an antiallergenic wallpaper (processed wallpaper). Each test subject was sufficiently infiltrated with the aqueous solution for evaluation by putting and lightly pressing. The contact amount of the mite allergen to the subject is 20 ng per 10 cm ² (see Table 3). Then, the test subject was stored in a sealed state at 20 ° C. for 1 hour. Thereafter, about 0.5 mL of the aqueous solution for evaluation was collected and centrifuged. Next, the obtained supernatant was applied to the above method to determine the residual mite allergen concentration per volume of the aqueous solution for evaluation used, and the allergen inactivation rate was measured. The results are shown in Table 3.

Example 2-2
The test solution (L16) obtained in Preparation Example 16 was used as an aqueous solution for evaluation, and the same operation as in Example 2-1 was performed except that 1.5 mL thereof was brought into contact with the subject. Obtained (see Table 3).

Comparative Example 2-1
The test solution (L17) obtained in Preparation Example 17 was used as an aqueous solution for evaluation, and the same operation as in Example 2-1 was performed except that 1.5 mL thereof was brought into contact with the subject. Obtained (see Table 3).

Comparative Example 2-2
The test solution (L18) obtained in Preparation Example 18 was used as an aqueous solution for evaluation, and the same operation as in Example 2-1 was performed except that 1.5 mL of the test solution was brought into contact with the subject, and the allergen inactivation rate was determined. Obtained (see Table 3).

According to Table 3, when the antiallergenicity of a subject is evaluated, an aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less is brought into contact with the subject as an aqueous solution for evaluation. It is clear that it is less affected. Moreover, anti-allergenicity could be more accurately evaluated by using a nonionic surfactant and BSA together.

According to the anti-allergen evaluation method of the present invention, it is possible to accurately evaluate the anti-allergen properties of a substrate before anti-allergen processing that does not inactivate allergens and anti-allergen processed products that have an allergen inactivation effect. it can. In this method, as the aqueous solution for evaluation, the test solution of the present invention can be used as it is or after diluting the test solution of the present invention if necessary, so that the test solution of the present invention is useful. According to the method for producing an anti-allergen processed product of the present invention, for example, a fiber material, a wood material used for flooring, a paper or plastic sheet used for wallpaper, a rubber or plastic sheet, glass or ceramics. It is possible to reliably obtain an anti-allergen processed product that is a building material such as a tile plate made of stone or stone and whose anti-allergen property is accurately evaluated.

Claims (12)

1. A test solution used in evaluating the antiallergenicity of a subject,
   A test solution containing an allergen and water.
2. The test solution according to claim 1, wherein the allergen concentration is 5 to 1000 ng / mL.
3. The test solution according to claim 1 or 2, comprising an allergen and water.
4. Furthermore, the test liquid as described in any one of Claim 1 to 3 containing a nonionic surfactant.
5. The test solution according to any one of claims 1 to 4, further comprising bovine serum albumin.
6. The test solution according to any one of claims 1 to 5, comprising an allergen, water, and at least one selected from a nonionic surfactant and bovine serum albumin.
7. In a method for evaluating the antiallergenicity of a subject,
   The test solution according to claim 1 or 2, or a diluted solution thereof, wherein an aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less is used.
8. The anti-allergenicity evaluation method according to claim 7, wherein the aqueous solution is contacted with the subject, then impurities are removed from the collected liquid, and then the allergen concentration in the obtained solution is measured.
9. The antiallergenicity according to claim 8, wherein when the aqueous solution is brought into contact with the subject, the contact amount of the allergen contained in the aqueous solution is 5 to 200 ng per unit area (10 cm ² ) of the subject. Evaluation methods.
10. In a method for evaluating the anti-allergen property of an anti-allergen processed product obtained by applying anti-allergen processing to a base material that has not been processed with anti-allergen,
    The test solution according to claim 1 or 2, or a diluted solution thereof, wherein an aqueous solution having an inorganic compound salt concentration of 0.04 mol / L or less is brought into contact with the anti-allergen processed product, and then contaminated from the recovered solution. objects were removed, then, by measuring the allergen concentration C ₂ in the resulting solution,
    The aqueous solution is contacted with the substrate, and then impurities are removed from the collected liquid, and then the allergen concentration C ₁ in the obtained solution is measured,
    The concentration C ₂ and using the concentration C _1, antiallergenic evaluation method characterized by obtaining the allergen deactivation rate.
11. When the aqueous solution is brought into contact with the anti-allergen processed product or the substrate, the contact amount of the allergen contained in the aqueous solution is 5 to 5 per unit area (10 cm ² ) of the anti-allergen processed product or the substrate. The antiallergenicity evaluation method according to claim 10, wherein the antiallergenicity is 200 ng.
12. An anti-allergen processing is performed on a base material that has not been anti-allergen-processed, and an anti-allergen property evaluation method according to claim 10 or 11 is used. A method for manufacturing allergen processed products.