JPH06261644A - Raising seedling of tissue culture-derived juvenile plant - Google Patents
Raising seedling of tissue culture-derived juvenile plantInfo
- Publication number
- JPH06261644A JPH06261644A JP8029493A JP8029493A JPH06261644A JP H06261644 A JPH06261644 A JP H06261644A JP 8029493 A JP8029493 A JP 8029493A JP 8029493 A JP8029493 A JP 8029493A JP H06261644 A JPH06261644 A JP H06261644A
- Authority
- JP
- Japan
- Prior art keywords
- tissue culture
- derived
- seedlings
- sugar
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、組織培養由来幼植物体
の育苗方法に関するものである。TECHNICAL FIELD The present invention relates to a method for raising seedlings of tissue culture-derived seedlings.
【０００２】[0002]
【従来の技術】従来の組織培養由来幼植物体の育苗方法
は、無菌有糖培地において幼植物体を独立栄養可能な状
態まで生育させた後、パーライト、鹿沼土、一般の土な
どに移植し、順化を行い、苗に育成していた。有糖培地
から培養土へ移植する工程を採用する理由は、有糖培地
は汚染しやすく、いったんコンタミネーションが蔓延す
ると幼植物体が全滅する場合もあり、その危険から植物
体を保護するためである。2. Description of the Related Art The conventional method for raising seedlings derived from tissue culture is to grow seedlings in a sterile sugar-containing medium to a state where they can be autotrophic, and then transplant them to perlite, Kanuma soil, or ordinary soil. , Acclimated, and had grown into seedlings. The reason for adopting the step of transplanting from the sugar-containing medium to the culture soil is that the sugar-containing medium is liable to be contaminated, and once the contamination has spread, the seedlings may be completely destroyed. is there.
【０００３】[0003]
【発明が解決しようとする問題点】前記したように従来
の組織培養由来幼植物体の育苗方法にあっては、無菌有
糖培地から一般の土などに移植する工程が不可欠であっ
たために、次のような問題点が存在した。すなわち移植
の際のショックやストレスによって植物体の生育阻害を
招き、枯死などが発生することを避けることができなか
った。[Problems to be Solved by the Invention] As described above, in the conventional method for raising seedlings derived from tissue culture, since the step of transplanting from a sterile sugar-containing medium to general soil is essential, There were the following problems. That is, it was unavoidable that shock and stress at the time of transplantation would lead to growth inhibition of the plant body and death.
【０００４】[0004]
【本発明の目的】本発明は上記したような従来の問題を
解決するためになされたもので、移植の際のショックや
ストレスを発生させず、効率よく植物体を育成すること
ができる、組織培養由来幼植物体の育苗方法を提供する
ことを目的とする。The object of the present invention is to solve the above-mentioned conventional problems, and it is possible to efficiently grow a plant without causing shock or stress during transplantation. It is an object to provide a method for raising seedlings of culture-derived seedlings.
【０００５】[0005]
【問題点を解決するための手段】上記のような目的を達
成するために、本発明の組織培養由来幼植物体の育苗方
法は、組織培養により誘導された幼植物体を、高濃度炭
酸ガスの環境下で無機養分を含む無糖ゲル状培地に置床
し、独立栄養が可能な状態まで育成した後、当該無糖ゲ
ル状培地上に培養土を直接覆土し、温室において順化苗
とする、方法を特徴としたものである。[Means for Solving the Problems] In order to achieve the above object, a method for raising seedlings of a tissue culture-derived seedling according to the present invention is a method in which seedlings induced by tissue culture are treated with high-concentration carbon dioxide gas. After placing in a sugar-free gel-like medium containing inorganic nutrients under the environment of, and growing to a state capable of autotrophic, cover the culture soil directly on the sugar-free gel-like medium and use it as an acclimated seedling in a greenhouse. , Characterized by the method.
【０００６】[0006]
【本発明の構成】以下本発明の組織培養由来幼植物体の
育苗方法について説明する。 ＜イ＞無糖ゲル培地 本発明の方法においては、従来の有糖培地に対して、特
に無糖ゲル状培地を使用する。そして、組織培養により
誘導された幼植物体を、高濃度炭酸ガスの環境下で無機
養分を含む無糖ゲル状培地に置床するものである。そし
てたとえば培養庫において５％の高濃度炭酸ガス施用に
より、２８℃において、１２，０００ルックスの光線を
２４時間照射して独立栄養可能な状態まで育成を行う。[Structure of the present invention] The method for raising seedlings of tissue culture-derived seedlings of the present invention will be described below. <a> Sugar-free gel medium In the method of the present invention, a sugar-free gel medium is used, in particular, in contrast to the conventional sugar-containing medium. Then, the seedlings induced by tissue culture are placed in a sugar-free gel medium containing inorganic nutrients in an environment of high concentration carbon dioxide. Then, for example, by applying a 5% high-concentration carbon dioxide gas in a culture cabinet, irradiation with a light beam of 12,000 lux is performed at 28 ° C. for 24 hours to grow to a state of being able to autotrophic.
【０００７】＜ロ＞覆土 次の工程において、本発明の方法においては移植を行
ず、移植に代えて覆土を行う。すなわち、幼植物体が独
立栄養成長が可能な状態まで育成したらその後に、移植
を行わずそのまま無糖ゲル状培地上に培養土を覆土す
る。覆土を行う培養土としては、パーライト、鹿沼土、
その他一般の土などを利用する。そして覆土を行ったの
ち、温室において生育させ、順化苗とする。<B> Soil Covering In the next step, transplantation is performed in the method of the present invention, and soil covering is performed instead of transplantation. That is, after the seedlings are grown to a state where autotrophic growth is possible, after that, the culture soil is covered directly on the sugar-free gel-like medium without transplanting. Culture soil for covering soil is perlite, Kanuma soil,
Other common soil is used. After covering with soil, the seeds are grown in a greenhouse to obtain acclimated seedlings.
【０００８】[0008]
【実施例１】本発明の方法と、従来の移植法との比較を
行った。すなわち組織培養により誘導された幼植物体
を、高濃度炭酸ガス施用により無機養分を含む無糖ゲル
状培地で２週間育苗した。そして１０ｃｍに生育した植
物体各１０本を次にケースに分けて観察した。 移植せずに培養土で覆土して一般温室で２週間育成。 培養土へ移植して一般温室で２週間育成。 その結果の平均生育量を示すと次の表１の通りである。Example 1 The method of the present invention was compared with the conventional transplantation method. That is, the seedlings induced by tissue culture were bred for 2 weeks in a sugar-free gel medium containing inorganic nutrients by applying high-concentration carbon dioxide. Then, 10 plants each grown to 10 cm were divided into cases and observed. Instead of transplanting, cover with culture soil and grow in a general greenhouse for 2 weeks. Transfer to culture soil and grow for 2 weeks in a general greenhouse. Table 1 below shows the average growth of the results.
【表１】 なお充実度とは地上部乾物重／草丈である。この表か
ら、移植しないで覆土して育苗したものが、草丈、地上
部乾物重、地下部乾物重ともに勝り、生育阻害を大幅に
減少させることができることが明かとなった。[Table 1] The degree of solidity is dry matter weight above ground / plant height. From this table, it was revealed that the seedlings that were covered with soil without transplanting were superior in plant height, dry weight above ground, and dry weight below ground, and could significantly inhibit growth inhibition.
【０００９】[0009]
【実施例２】組織培養により誘導された幼植物体を各１
７０本をづつ、高濃度炭酸ガス施用により無機養分を含
む無糖ゲル状培地で２週間育苗した。その後、次のケー
スに分けて観察した。 移植せずに培養土で覆土して一般温室で２週間育成。 培養土へ移植して一般温室で２週間育成。 その結果の平均生育量を示すと、表２の通りである。[Example 2] 1 seedling derived from each tissue culture
Seventy seedlings were cultivated for 2 weeks in a sugar-free gel medium containing inorganic nutrients by applying high-concentration carbon dioxide. Then, the observation was made in the following cases. Instead of transplanting, cover with culture soil and grow in a general greenhouse for 2 weeks. Transfer to culture soil and grow for 2 weeks in a general greenhouse. Table 2 shows the average growth of the results.
【表２】 この表から、移植しないで覆土して育苗したことにより
活着率を向上させ、生育阻害を大幅に減少させることが
できることが明かとなった。[Table 2] From this table, it was revealed that covering the soil without transplanting and raising the seedlings can improve the survival rate and significantly reduce the growth inhibition.
【００１０】[0010]
【発明の効果】本発明の組織培養由来幼植物体の育苗方
法は以上説明したように、無糖培地において育成させた
幼植物体を、移植することなしにそのまま覆土して育苗
する方法である。その結果、移植して行う従来の方法に
比較して、活着率が向上し、草丈、地上部乾物重、地下
部乾物重ともに勝り、生育阻害を大幅に減少させること
ができることが明かとなった。 整理番号９３０−２４７
１／１化学式を記載した書面明細書Industrial Applicability As described above, the method for raising seedlings of tissue culture-derived seedlings of the present invention is a method of raising seedlings by directly covering the seedlings grown in a sugar-free medium without transplanting them. . As a result, it was revealed that the survival rate was improved and both plant height, aboveground dry weight and belowground dry weight were superior, and growth inhibition could be significantly reduced compared to the conventional method of transplanting. . Reference number 930-247
1/1 Written statement with chemical formula
【表１】 移植無し覆土 移植 草丈（cm） 34.6 24.3 地上部乾物重（mg） 75.2 24.9 充実度（mg/cm) 2.17 1.02 地下部乾物重（mg） 31.4 6.6 [Table 1] Non-transplanted soil cover Transplanted plant height (cm) 34.6 24.3 Aboveground dry weight (mg) 75.2 24.9 Solidity (mg / cm) 2.17 1.02 Underground dry matter (mg) 31.4 6.6
【表２】 [Table 2]
Claims (1)
濃度炭酸ガスの環境下で無機養分を含む無糖ゲル状培地
に置床し、 独立栄養可能な状態まで育成した後、当該無糖ゲル状培
地上に直接培養土を覆土し、 温室において順化苗とする、 組織培養由来幼植物体の育苗方法1. A seedling induced by tissue culture is placed in a sugar-free gel medium containing inorganic nutrients in a high-concentration carbon dioxide environment and grown to a state of being able to autotrophically, and then the sugar-free. Seedling raising method for tissue culture-derived seedlings by covering culture soil directly on gel medium and acclimatizing seedlings in a greenhouse
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8029493A JPH06261644A (en) | 1993-03-16 | 1993-03-16 | Raising seedling of tissue culture-derived juvenile plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8029493A JPH06261644A (en) | 1993-03-16 | 1993-03-16 | Raising seedling of tissue culture-derived juvenile plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06261644A true JPH06261644A (en) | 1994-09-20 |
Family
ID=13714256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8029493A Pending JPH06261644A (en) | 1993-03-16 | 1993-03-16 | Raising seedling of tissue culture-derived juvenile plant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06261644A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039368A (en) * | 2013-01-27 | 2013-04-17 | 云南天泉生物科技股份有限公司 | Sugar-free tissue culture method of dendrobium officinale |
| CN115486360A (en) * | 2022-08-29 | 2022-12-20 | 甘肃勇馨科技农业有限公司 | Novel sugar-free efficient rooting culture and transplanting method for detoxified ginseng fruit seedlings |
-
1993
- 1993-03-16 JP JP8029493A patent/JPH06261644A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039368A (en) * | 2013-01-27 | 2013-04-17 | 云南天泉生物科技股份有限公司 | Sugar-free tissue culture method of dendrobium officinale |
| CN115486360A (en) * | 2022-08-29 | 2022-12-20 | 甘肃勇馨科技农业有限公司 | Novel sugar-free efficient rooting culture and transplanting method for detoxified ginseng fruit seedlings |
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