JPH06253819A - Method of culturing plankton - Google Patents
Method of culturing planktonInfo
- Publication number
- JPH06253819A JPH06253819A JP4786193A JP4786193A JPH06253819A JP H06253819 A JPH06253819 A JP H06253819A JP 4786193 A JP4786193 A JP 4786193A JP 4786193 A JP4786193 A JP 4786193A JP H06253819 A JPH06253819 A JP H06253819A
- Authority
- JP
- Japan
- Prior art keywords
- plankton
- ozone
- culture
- medium
- culturing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明はプランクトンの培養方法
に関する。FIELD OF THE INVENTION The present invention relates to a method for culturing plankton.
【０００２】[0002]
【従来の技術】従来、例えばクロレラなどの有用プラン
クトンを培養増殖する場合、その増殖速度を向上させる
ために当該プランクトンの最適培地となるよう培地組
成を調整する。光合成を促すため、照射光量を増加す
る。電磁場をかけ体内取込みのためのイオン移動を促
進させるなどの試みがなされてきたが、その効果は数割
程度しか認められなかった。2. Description of the Related Art Conventionally, when a useful plankton such as chlorella is grown in culture, the composition of the medium is adjusted so as to be the optimum medium for the plankton in order to improve its growth rate. The amount of irradiation light is increased to promote photosynthesis. Attempts have been made to apply an electromagnetic field to accelerate the movement of ions for uptake in the body, but the effect was only recognized to be about a few percent.
【０００３】[0003]
【発明が解決しようとする課題】培地組成を調整したり
照射光量を増加しても、直接生合成に関与する酵素など
への働きかけでないため、初期には若干の増殖速度の向
上が認められるも長期間のうちに低下してくる。また長
期間の培養中には大気中の浮遊細菌の混入により全く増
殖しなくなることもある。また、照射光量の増加や電磁
場の印加は設備投資も高く、またランニングコストも高
くなるにもかゝわらず、増殖速度向上も僅かに過ぎな
い。従って、イニシャルコスト、ランニングコストの安
い高速培養方法が望まれている。Even if the composition of the medium is adjusted or the irradiation light amount is increased, it does not affect the enzymes directly involved in biosynthesis, so that the growth rate is slightly improved in the initial stage. It will decrease over a long period of time. Further, during long-term culture, it may not grow at all due to contamination with airborne bacteria. Further, although the increase of the irradiation light amount and the application of the electromagnetic field require a high capital investment and a high running cost, the growth rate is only slightly improved. Therefore, a high-speed culture method with low initial cost and running cost is desired.
【０００４】本発明は上記技術水準に鑑み、安価な設備
により、プランクトンを高速培養できる方法を提供しよ
うとするものである。In view of the above-mentioned state of the art, the present invention intends to provide a method capable of culturing plankton at high speed with inexpensive equipment.
【０００５】[0005]
【課題を解決するための手段】本発明は （１）プランクトン培養開始時及び培養期間中に、オゾ
ンを培地中に定期的に注入することを特徴とするプラン
クトンの培養方法。 （２）オゾン注入後、還元剤を添加することを特徴とす
る上記（１）記載のプランクトンの培養方法。である。The present invention provides (1) a plankton culturing method characterized in that ozone is periodically injected into a medium at the start and during the plankton culturing. (2) The plankton culturing method according to (1) above, wherein a reducing agent is added after ozone injection. Is.
【０００６】本発明において、培地に注入するオゾンは
オゾン水の状態でもオゾンガスの状態の何れでもよい。
また、添加される還元剤としてはチオ硫酸ソーダ、亜硫
酸ソーダ、亜硫酸カルシウム、ヒドラジンなどが一般的
に使用しうる。In the present invention, ozone injected into the medium may be in the state of ozone water or in the state of ozone gas.
As the reducing agent to be added, sodium thiosulfate, sodium sulfite, calcium sulfite, hydrazine and the like can be generally used.
【０００７】[0007]
【作用】バクテリア（病原性細菌）のオゾン耐性はプラ
ンクトンより弱いので、プランクトン培養開始時及び培
養期間中に定期的にオゾンを培地中に注入することによ
りバクテリアのみを選択的に殺菌しプランクトンのみを
純粋培養することが可能となり、そのため成長速度が促
進される。[Function] Since ozone resistance of bacteria (pathogenic bacteria) is weaker than that of plankton, by injecting ozone into the medium periodically at the start of plankton culture and during the culture period, only bacteria are selectively sterilized to remove only plankton. Pure culture is possible, which accelerates the growth rate.
【０００８】また、オゾンは遺伝情報を担う核酸（ＤＮ
Ａ）と反応し、遺伝子の変化を生じさせたり、酵素を活
性化することにより増殖速度を促進する。しかし、長時
間、オゾンやその副生物であるオキシダントにさらされ
ているとプランクトンの増殖に支障をきたすようにな
る。すなわち、その殺菌作用のため増殖しなくなる。そ
こである時間経過後には残留オゾン及びオキシダントを
除去する必要が生じる。この対策として還元剤を添加す
ることにより残留オゾン及びオキシダントを分解させ
る。Ozone is a nucleic acid (DN) that carries genetic information.
It reacts with A) to induce a gene change or activate an enzyme to accelerate the growth rate. However, if it is exposed to ozone or its by-product oxidant for a long period of time, it will hinder the growth of plankton. That is, it does not grow due to its bactericidal action. Therefore, after a certain period of time, it becomes necessary to remove residual ozone and oxidant. As a countermeasure against this, residual ozone and oxidant are decomposed by adding a reducing agent.
【０００９】[0009]
【実施例】植物性プランクトン、ドナリエラ（ D. sali
na １９／３０）を、栄養源ＫＨ 2 ＰＯ4 ４．５ｍｇ／
リットル、ＫＮＯ3 ７２ｍｇ／リットルを添加した海水
培地を用い、２３℃で蛍光灯（昼光色）を照射し空気を
通気しながら７日間培養を行った。Example: Phytoplankton, Donaliella (D. sali
na 19/30) as a nutrient source KH 2POFour4.5 mg /
Liter, KNO3Seawater with 72 mg / liter added
Using a culture medium, irradiate a fluorescent lamp (daylight color) at 23 ° C to blow air.
Culture was performed for 7 days while aerating.
【００１０】オゾンは培養開始時、培地中の濃度が０．
１、０．５、１、２ｐｐｍとなるように高濃度オゾン水
を培地に滴下攪拌した。この時のドナリエラの増殖特性
を図１に示す。図中の吸光度とプランクトン濃度の関係
は図２に示すとおりである。Ozone has a concentration of 0.
High-concentration ozone water was added dropwise and stirred to the medium so as to be 1, 0.5, 1, 2 ppm. The growth characteristics of Donaliella at this time are shown in FIG. The relationship between the absorbance and the plankton concentration in the figure is as shown in FIG.
【００１１】図１からオゾン無注入の場合に比べ、オゾ
ン注入濃度が０．１〜１．０ｐｐｍの範囲では注入濃度
の増加とともにプランクトン増殖速度も向上しているこ
とが判る。すなわち長期間、高増殖速度が維持できる。
オゾン注入濃度が２ｐｐｍを超えると、培養初期に増殖
誘導期間が生じるが、やがて増殖し始めるとその増殖速
度はオゾン注入濃度１．０ｐｐｍの場合とほぼ同程度に
なる。従ってドナリエラの場合オゾン注入濃度は１ｐｐ
ｍが最適である。It can be seen from FIG. 1 that the plankton growth rate is improved with the increase of the injection concentration in the range of the ozone injection concentration of 0.1 to 1.0 ppm as compared with the case of no ozone injection. That is, a high growth rate can be maintained for a long period of time.
When the ozone injection concentration exceeds 2 ppm, a growth induction period occurs in the early stage of culture, but when the ozone injection concentration starts, the growth rate is almost the same as when the ozone injection concentration is 1.0 ppm. Therefore, in the case of Donariella, the ozone injection concentration is 1 pp
m is optimal.
【００１２】オゾンの間欠注入効果を図３に示す。図３
より判るように、オゾン注入頻度は１回／７日の方が１
回／１日、１回／２日に比べプランクトン増殖速度が速
い。従ってオゾン注入頻度は少なくてよく、一週間に１
回程度でよい。The effect of intermittent injection of ozone is shown in FIG. Figure 3
As can be seen, the frequency of ozone injection is once every 7 days.
Plankton growth rate is faster than once / day and once / two days. Therefore, the frequency of ozone injection may be low, and it may be 1 per week.
Only about once.
【００１３】またドナリエラから有用成分であるβ−カ
ロチンを抽出する場合は、更にオゾン曝露時間を制限し
た方がよい。この方法としてオゾン注入５分後にＮａ2
Ｓ2Ｏ3 を添加した場合の効果を図４に示す。図４より
判るように、オゾン無注入の場合に比べ、オゾン１ｐｐ
ｍ注入するとβ−カロチン生成量は７日間で約５倍、更
にオゾン注入５分後にＮａ2 Ｓ2 Ｏ3 を０．１ｐｐｍ添
加した場合は約８倍となる。Further, when β-carotene, which is a useful component, is extracted from Donaliella, it is better to further limit the ozone exposure time. As this method, Na 2 was injected 5 minutes later.
The effect of adding S 2 O 3 is shown in FIG. As can be seen from FIG. 4, compared to the case of no ozone injection, ozone is 1 pp
When m is injected, the amount of β-carotene produced is about 5 times in 7 days, and when Na 2 S 2 O 3 is added at 0.1 ppm 5 minutes after the injection of ozone, the amount is about 8 times.
【００１４】[0014]
【発明の効果】本発明によれば、プランクトンの純粋培
養が可能となるので、プランクトンの増殖速度を向上さ
せることができ、またオゾン曝露時間を制限することに
より増殖プランクトンにより大量の有効成分（例えばβ
−カロチン）を抽出しうることが可能となる。EFFECTS OF THE INVENTION According to the present invention, since it is possible to purely culture plankton, it is possible to improve the growth rate of plankton, and by limiting the ozone exposure time, a large amount of active ingredients (for example, a plankton) can be obtained. β
-Carotene) can be extracted.
【図１】本発明の一実施例のドナリエラ増殖特性を示す
図表。FIG. 1 is a chart showing Donariella growth characteristics of one example of the present invention.
【図２】吸光度とプランクトン濃度の関係を示す図表。FIG. 2 is a chart showing the relationship between absorbance and plankton concentration.
【図３】本発明の一実施例のオゾンの間欠注入効果を示
す図表。FIG. 3 is a diagram showing the effect of intermittent injection of ozone according to an embodiment of the present invention.
【図４】本発明の一実施例のβ−カロチンの生成量をを
示す図表。FIG. 4 is a chart showing the amount of β-carotene produced in one example of the present invention.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 滝村 修 広島県呉市広末広２丁目２番２号 工業技 術院 中国工業技術試験所内 (72)発明者 大田 利行 広島県広島市西区観音新町四丁目６番22号 三菱重工業株式会社広島研究所内 (72)発明者 榎本 智之 広島県広島市西区観音新町四丁目６番22号 三菱重工業株式会社広島製作所内 ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Osamu Takimura 2-2-2 Hirosuehiro, Kure City, Hiroshima Prefecture Industrial Technology Institute, China Industrial Technology Laboratory (72) Toshiyuki Ota, Kannon Shinmachi, Nishi-ku, Hiroshima City, Hiroshima Prefecture 6-22 No. 6 Hiroshima Research Laboratory, Mitsubishi Heavy Industries, Ltd. (72) Inventor Tomoyuki Enomoto 4-6-22 Kannon Shinmachi, Nishi-ku, Hiroshima City, Hiroshima Prefecture Mitsubishi Heavy Industries Ltd. Hiroshima Works
Claims (2)
に、オゾンを培地中に定期的に注入することを特徴とす
るプランクトンの培養方法。1. A method for culturing plankton, which comprises periodically injecting ozone into a medium at the start and during the culturing of plankton.
特徴とする請求項１記載のプランクトンの培養方法。2. The method for culturing plankton according to claim 1, wherein a reducing agent is added after ozone injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4786193A JPH06253819A (en) | 1993-03-09 | 1993-03-09 | Method of culturing plankton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4786193A JPH06253819A (en) | 1993-03-09 | 1993-03-09 | Method of culturing plankton |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06253819A true JPH06253819A (en) | 1994-09-13 |
Family
ID=12787158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4786193A Pending JPH06253819A (en) | 1993-03-09 | 1993-03-09 | Method of culturing plankton |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06253819A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1084800A (en) * | 1996-09-19 | 1998-04-07 | Sanyo Electric Co Ltd | Culture device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03130070A (en) * | 1988-09-20 | 1991-06-03 | Fumio Onuki | Culture of unicellular green alga r. sager strain 95 belonging to genus chlamydomonas and culture device therefor |
| JPH03186390A (en) * | 1989-12-15 | 1991-08-14 | Agency Of Ind Science & Technol | Sea water sterilization using ozone |
| JPH03270793A (en) * | 1990-03-20 | 1991-12-02 | Fumio Onuki | Method for obtaining drinking water using unicellular green algae belonging to genus chlamydomonas from dirty water of rivers or lakes and marshes |
| JPH06226273A (en) * | 1993-02-03 | 1994-08-16 | Mitsubishi Heavy Ind Ltd | Ozone sterilization method for aqueous solution system |
-
1993
- 1993-03-09 JP JP4786193A patent/JPH06253819A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03130070A (en) * | 1988-09-20 | 1991-06-03 | Fumio Onuki | Culture of unicellular green alga r. sager strain 95 belonging to genus chlamydomonas and culture device therefor |
| JPH03186390A (en) * | 1989-12-15 | 1991-08-14 | Agency Of Ind Science & Technol | Sea water sterilization using ozone |
| JPH03270793A (en) * | 1990-03-20 | 1991-12-02 | Fumio Onuki | Method for obtaining drinking water using unicellular green algae belonging to genus chlamydomonas from dirty water of rivers or lakes and marshes |
| JPH06226273A (en) * | 1993-02-03 | 1994-08-16 | Mitsubishi Heavy Ind Ltd | Ozone sterilization method for aqueous solution system |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1084800A (en) * | 1996-09-19 | 1998-04-07 | Sanyo Electric Co Ltd | Culture device |
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Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 19960305 |