JPH06247786A - Good quality maturation of livestock effluent rubbish - Google Patents

Abstract

PURPOSE:To carry out good quality maturation of a livestock effluent rubbish by utilizing a fermentation promoting fertilizer or microorganisms. CONSTITUTION:Good quality maturation of a livestock effluent rubbish can be carried out by adding an acetoaldehyde processed urea, dried blood and superphosphate of lime or further adding cellulose decomposing bacteria to the livestock effluent rubbish and by allowing it to ferment.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to high-quality ripening of livestock waste (manure). More specifically, it relates to a method for aging the livestock waste with good quality by adding acetaldehyde-processed urea, dry blood, lime superphosphate, or a cellulose-degrading bacterium together with these to the livestock waste.

[0002]

2. Description of the Related Art In dairy farms, rice straw, wheat culm, bark,
Sawdust, etc. is laid inside the livestock shed, carried out with the excrement of livestock outside the livestock shed and accumulated for a certain period of time, and then returned to the fields. Since these wastes have a high water content and a large amount of organic substances such as rice straw, wheat culm, bark, and sawdust, fermentation does not proceed and remains immature. Therefore, crops cultivated in the fields to which these wastes have been applied are of poor quality and yield is reduced. Furthermore, pollution of environment and water quality has become a problem.

[0003]

Under such circumstances, a method of aging livestock waste to a high quality is urgent and strongly desired.

[0004]

[Means for Solving the Problems] The present inventor has been studying high-quality ripening of compost, and has already filed a patent application (Japanese Patent Application No. 3-361527) for high-quality ripening of compost. After that, we screened the livestock waste for microorganisms capable of degrading cellulose, and worked on high-quality ripening of livestock waste using this microorganism. In addition to nitrogen fertilizer, phosphate fertilizer was added as a fermentation promoting material to prevent the growth of denitrifying bacteria during the fermentation process. as a result,
The present invention was completed by discovering that livestock wastes can be ripened with good quality by adding acetaldehyde-processed urea, dry blood and lime superphosphate, or by adding cellulose-decomposing bacteria to the livestock wastes and fermenting them together. Let

That is, the present invention is characterized in that acetaldehyde-processed urea, dry blood and lime superphosphate, or a cellulolytic bacterium together with these, is added to fermenting livestock waste to ferment it. It is a chemical method. The livestock waste targeted by the present invention is generally known livestock (cattle,
A mixture of manure of pigs, horses, chickens) and organic matter laid on the floor of a barn, such as rice straw, wheat culm, bark, sawdust, etc., in any combination.

The acetaldehyde-processed urea to be added to livestock waste is 2-oxo-4-methyl-6-ureidohexahydropyrimidine, which is widely used as trade name-CDU. Dry blood is usually used as an organic nitrogen fertilizer, and any kind of dry blood may be used as long as it is dried. Lime superphosphate is also often used as a phosphate fertilizer, and a commercially available product can be used as it is.

The mixing ratio of acetaldehyde-processed urea, dry blood and lime superphosphate is usually 64 to 70:15, respectively.
-18: 15-18% by weight, preferably 68:16:
16% by weight. The amount of this mixture added to livestock waste is usually 0.4 to 0.8% by weight, preferably 0.5 to 0.6% by weight, based on the livestock waste. The addition timing is not particularly limited and may be any, for example, it is added to the livestock waste when it is sprayed on the organic matter laid on the floor before carrying it out of the livestock house or when it is cut back after natural deposition. can do.

When the livestock waste obtained by adding the above-mentioned mixture is applied to soil, it has the effect of promoting the growth of crops. It also increases total nitrogen and total phosphoric acid in livestock waste. Further, the carbon / nitrogen value, which is an index of the progress of ripening of compost, was also improved, and the effect of good ripening was clearly recognized. In the present invention, a cellulolytic bacterium may be added together with acetaldehyde-processed urea, dry blood and lime superphosphate. Such a bacterium is M
Bacteria belonging to the genus Yrothecium are mentioned. Among them, My, a strain newly isolated from livestock waste
rosetheum sp. SF-M-001 is preferred. This strain was deposited on February 1, 1993 at the Institute of Biotechnology, Institute of Industrial Science and Technology, and the deposit number is FERM.
P-13387 is added. When such a bacterium is actually added, a culture obtained by depositing, for example, rice bran: vermiculite (3: 7) medium is used as described above.
Usually 1-2% by weight, preferably 1.4-
It is 1.6% by weight. The timing of adding the bacteria to the above-mentioned three types of materials is not particularly limited and may be the time of mixing the three types of materials. The timing and method of applying to livestock waste are the same as above.

The livestock waste obtained by adding the bacterium can bring about a better quality ripening than that without the bacterium. In order to ferment livestock wastes by adding the above-mentioned three kinds of fertilizers or cellulolytic bacteria, field accumulation or natural accumulation as usual may be carried out, or fermentation may be carried out after being accumulated in a fermentation layer.

[0010]

As described above, according to the present invention, high-quality ripening of livestock waste can be performed. When the livestock waste obtained by the present invention is applied to agricultural crops, it promotes the growth of agricultural crops and has the effect of increasing the yield, and therefore has a great significance in contributing to the waste treatment of dairy farmers and the development of agriculture.

[0011]

EXAMPLES The present invention will now be described in more detail with reference to examples. Example 1 Preparation of high-quality ripening material for livestock waste 20 parts by weight of acetaldehyde-processed urea 2-oxo-4-methyl-6-ureidohexahydropyrimidine, dry blood, and 5 parts by weight of lime superphosphate were measured. , A material for aging the livestock waste to a high quality is obtained by uniformly mixing with a rocking mixer.

Example 2 Preparation of high-quality ripening material for livestock waste containing cellulose-degrading bacteria Rice bran and vermiculite were mixed in a volume ratio of 3: 7, sterilized in an autoclave by adding appropriate water, and then screened in Example 3. Obtained by Myrothecium sp. The bacteria were planted and cultured in a thermostat at 25 ° C for 2 weeks. This culture is added to and mixed with the material prepared in Example 1 at 1.5% by weight to obtain a material that ripens livestock waste to a good quality.

Example 3 Screening of Cellulolytic Bacteria Livestock waste during spontaneous sedimentation fermentation was sampled from a depth of 30 cm to obtain CMC (Carboxymethy).
l cellulose) medium (NH ₄ NO ₃ 0.3
_{g, MgSO 4 · 7H 2 O} 0.5g, K 2 HPO 4
_{1.0g, KCl0.5g, Fe 2 (SO} 4) 3 · 7H
₂ O 10 mg, CMC-Na 5.0 g, agar 18 g,
The microorganisms were separated by the dilution plate method using distilled water (1000 ml), and the colonies appearing on the plate were transferred to a potato decoction slope medium and stored. These microorganisms were planted again in the center of a petri dish in which CMC medium was poured,
After culturing at 28 ° C. for 5 days, 5 cc of a 1% cetyltrimethylammonium bromide solution was poured thereinto to screen for cellulolytic bacteria forming a clear zone. As a result, cellulolytic bacteria of 6 bacterial strains, 32 actinomycetes strains and 8 filamentous fungi strains were obtained. Among them, filamentous fungus Myr
The clear zone formed by Othecium sp was the largest. A strain that is one of the filamentous fungi is Myrot
hecium sp. It was named SF-M-001, and was deposited at the Institute of Biotechnology, Institute of Biotechnology, Institute of Industrial Science, dated February 1, 1993, and FERM P-13387 is given as a deposit number. This strain grows well on the potato dextrose agar medium shown below, forming cushion-like conidia, and occasionally forming transparent bristles around the conidia. Moreover, the conidia peduncle is repeatedly branched. Conidia are unicellular, translucent to dark, and oval elongated. Medium name: potato dextrose agar medium BECTON DICKINSON Infusion medium composition: Potato, Infusion from (Solid) 4.0 g Dextrose 20.0 g Agar 15.0 g (per 100 ml of medium) pH of medium: 5.6 ± 0.2 of medium Sterilization conditions: 121 ° C. for 15 minutes Culture temperature: 25 ° C. Culture period: 6 days Strain Myrothecium sp. SF-M-001
Oxygen requirement was aerobic.

Example 4 Decomposition of Myrothecium sp on rice straw
Noh Experiment "Latest Microbial Handbook" (Yoshiro Okami et al. P.457-
458) Sasa powder decomposition test method according to Myrthe
The ability of Cium sp to degrade rice straw was investigated.
Lentinus ododes (Shiitake bacterium) was used as a control bacterium.

[0015]

[Table 1] As shown in Table 1, My screened in Example 3
The ability of R. bacterium to degrade rice straw is:
Obviously, and clearly stronger than Shiitake.

Example 5 Experiment of ripening of livestock waste of high-quality ripening material Waste of dairy cattle laid with bark, rice husk and rice straw was collected and put into 1 kg of waste (water content 80 ± 2%). 5 g of the ripening material prepared in 1 was added. These wastes were put in a enamel pot with a diameter of 30 cm and a depth of 15 cm, covered with caps, and forcedly fermented in a thermostat at 50 ° C., and the effect of the materials of Example 1 on the ripening of the waste was examined. The effect on ripening is the yield of waste (decomposition reduction rate) after 2 months of fermentation.
And the content of 3 elements in the waste was investigated.

[0017]

[Table 2]

The results obtained are shown in Table 2 and FIG. Table 2
As shown in, the content of the three elements of the livestock waste fermented by adding the ripening material of the present invention was increased, and it was shown that ripening was carried out to a higher quality. Further, as shown in FIG. 1, the amount of waste to which the ripening material was added was smaller, suggesting that ripening proceeded.

Example 6 Effect of Fermented Livestock Waste on Crop Growth The high-quality ripened livestock waste of the present invention obtained in Example 5 was applied to soil to perform a test on the growth of crops. Diameter 15
1 liter of diluvium soil was packed in a unglazed pot of cm, and the livestock waste obtained in Example 5 was added and mixed so as to be 3 ton per 10 a. Twenty days radish was used as a trial product, and 5 per 1 pot
Seeded. The survey was conducted on the 30th day after seeding and 10
Two repetitions of the pot were done.

[0020]

[Table 3] The results obtained are shown in Table 3. As shown in Table 3, plant height, fresh weight, root weight, etc. showed remarkable growth in the livestock waste fermented by adding the high-quality ripening material obtained in the present invention.

Example 7 of high-quality ripening material and ripening material with addition of cellulolytic bacteria
Experiment of ripening of livestock waste To the waste of dairy cattle laid with wheat culm, 25 kg of the ripening material prepared in Examples 1 and 2 of the present invention was added per 5 tons of waste, and the raw material was taken out from the livestock barn and loaded in a field. , Covered with a sheet and fermented and ripened.

Example 8 Effect of Fermented Livestock Waste on Growth of Cucumber Seedlings Dipping soil was packed in a container having a width of 32 cm, a length of 47 cm and a depth of 6 cm, and 450 g of the livestock waste obtained in Example 7 ( 1
30 tons of cucumber seeds were sown by adding and mixing 3 tons per 0a). The survey was carried out on the 24th day after seeding, and each group was repeated twice. The results obtained are shown in Table 4.

[0023]

[Table 4]

The plant height, the number of leaves, the fresh weight and the dry weight were examined, and all of them were compared with the conventional plot (no application plot), and the soil to which the livestock wastes fermented by mixing the good quality ripening material of the present invention were applied was applied. , The growth of cucumber seedlings was excellent, and the material containing cellulose-decomposing bacteria was superior.

[Brief description of drawings]

FIG. 1 is a graph showing the yield rate of livestock waste produced by the use of ripening materials.

Claims (3)

[Claims] 1. A high-quality ripening method for livestock waste, which comprises adding acetaldehyde-processed urea, dry blood and lime superphosphate to the livestock waste and fermenting it. 2. The high-quality ripening method for livestock waste according to claim 1, wherein a cellulolytic bacterium is added together with acetaldehyde-processed urea, dry blood and lime superphosphate. 3. A cellulolytic bacterium is Myretheci.
The high-quality ripening method of livestock waste according to claim 2, which is a bacterium belonging to the genus um .