JPH0593729A - Automatic analysis method and apparatus - Google Patents

Abstract

PURPOSE:To achieve efficiency improvement and optimization of automatic analysis with higher operability and accuracy by arranging a sample container holding a reference substance solution of a high density and an empty sample container in a set to prepare the reference substance solution different in the density automatically. CONSTITUTION:A reference substance solution of a high density held in one sample container 2 is sucked and extruded in a sample container housing section 1 and dispensed into an empty container 2 together with water with a sample dispenser 4 which extracts a dilution liquid to be used as water to prepare a plurality of dilution reference substance solutions different in density sequentially. Then, a sample in the container 2 is sucked with the device 4 and moved horizontally to be dispensed into a reaction container 9. After the movement 11 of the container 9 to a specified position of a reaction table 12, a first reagent 17 and a second reagent corresponding to analysis items of the containers 9 are dispensed 19 and 20. ' Then, a reaction level is measured about all dilution separately reference substance solutions as preset with a photometric device 14 to prepare an expression usable as calibration value from a n-elationship between the resulting measurement data and a density value preset.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic analysis method, and more particularly to an automatic analysis method for preparing a calibration curve using a plurality of reference substances having different concentrations.

[0002]

2. Description of the Related Art In a conventional automatic analysis method, a calibration curve is prepared by using one reference substance for determining an analysis value. Here, the calibration curve refers to a characteristic curve obtained by using a standard sample or the like to determine the relationship between the amount or concentration of the target substance and the measured value in the analysis. When performing automatic analysis,
Since a simple chemical reaction or enzymatic reaction has a linear reaction range over a relatively wide range, there is no problem with a calibration curve prepared using only one reference substance.

[0003]

However, there are few immunoassays which have recently attracted attention, which have a linear response in a wide range. Therefore, up to now, a plurality of reference substances having different concentrations were prepared in advance, and the calibration curve was created by measuring them. However, the method of preparing a plurality of reference substances and performing the automatic analysis as described above has a problem that the handling becomes complicated and the cost becomes high. Further, when the inspector himself creates a plurality of reference substances, the inspectors vary in the production of the reference substances, which causes a problem in the accuracy of the reference substance preparation.

The present invention is proposed to solve the above problems, and an object of the present invention is to provide an automatic analysis method capable of easily performing automatic analysis and obtaining an appropriate automatic analysis result. It was done.

[0005]

In order to achieve the above-mentioned object, the present invention provides an automatic analysis method for preparing a calibration curve using a plurality of reference substances having different concentrations, and a set reference substance solution and a diluted solution. After preparing multiple diluted reference substance solutions with water and performing ordinary analysis, the measured value and the concentration are determined by those measured values by analysis and the concentration value of the reference substance in the diluted reference substance solution stored in advance. The automatic analysis method was used to create a calibration curve showing the relationship between values. In addition, in an automatic analyzer that creates a calibration curve using a plurality of reference substances with different concentrations, a sample container rack containing a reference substance solution and an empty sample container is provided, and a diluent and reference substance are provided. A sample dispenser for dispensing a solution and a sample dispenser was provided, and an automatic analyzer was prepared so as to prepare a plurality of dilution standard substance solutions.

[0006]

With the above-mentioned structure, the present invention only requires setting the most concentrated reference substance solution and an empty container,
Since the analyzer automatically prepares the diluted reference substance solutions having different concentrations, the operability is improved and the accuracy is improved.

[0007]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view of an analyzer used in the automatic analysis method according to the present invention. A sample rack 3 holding a large number of sample containers 2 is detachably provided in the sample container storage unit 1. The sample rack 3 can be moved in the front-rear direction (width direction) of the apparatus by a drive mechanism (not shown). It is like this. A sample dispensing device 4 for dispensing a sample is provided above the sample container storage portion 1, and its nozzle 5 can be moved horizontally and vertically via a rail 6. ing. Further, the nozzle 5 can be cleaned by a nozzle cleaning unit 7 provided in the horizontal movement direction. Since well-known moving mechanisms for the sample rack 3 and the sample dispensing device 4 are used, detailed description thereof will be omitted.

A reaction container storage portion 8 is provided near the sample storage portion 1, and a reaction container rack 10 holding a large number of reaction containers 9 is detachably provided in the reaction container storage portion 8. .. The reaction container rack 10 can be moved in the front-rear direction (width direction) of the apparatus by a drive mechanism (not shown). Above the reaction container accommodating portion 8, there is provided a reaction container transfer device 11 for gripping and lifting the reaction container 9 and transferring it while moving horizontally via the rail 6. The reaction container storage section 8 is maintained at a constant temperature which is almost equal to that for a reaction table described later or a predetermined temperature for each item by a thermostat (not shown).

A reaction table 12 is provided in the vicinity of the reaction container accommodating portion 8 and is adapted to rotate according to a designated sequence. The entire circumference of the reaction table 12 is kept constant by a constant temperature device (not shown).
In addition, above the reaction table 12, a stirring device 13 for promoting the reaction of the reaction liquid in the reaction container 9 located inside the reaction table 12 at an appropriate timing, and a photometric device for detecting the reaction degree. A device 14 is provided.

A first reagent storage portion 15 and a second reagent storage portion 16 are provided near the reaction table 12.
In this embodiment, the first reagent and the second reagent are set in their respective storages, but a large number of reagents such as the third reagent and the fourth reagent are set as needed. You can also A first reagent container 17 corresponding to an analysis item can be set in the first reagent storage unit 15, and a second reagent container 18 corresponding to an analysis item can be set in the second reagent storage unit 16. There is. Further, the first reagent container 17 and the second reagent container 18 can be moved in the front-rear direction (width direction) of the device by a drive mechanism (not shown).

Above the first reagent storage portion 15 and the second reagent storage portion 16, a first reagent dispensing device 19 for dispensing the first reagent and a second reagent for dispensing the second reagent. A dispensing device 20 is provided. The nozzles 21 and 22 of each of the above dispensing devices can be moved in the horizontal direction and the vertical direction via the rail 6. The nozzles 21 and 22 are cleaned by a nozzle cleaning unit 23 provided in the horizontal movement direction of the nozzles 21 and 22 before or after the reagent is dispensed.

In the thus constructed analyzer, first, the sample dispenser 4 sucks the sample contained in the sample container 2 of the sample container accommodating portion 1. Sample dispenser 4 when aspirated
The nozzle 5 moves horizontally and dispenses into the reaction container 9 of the reaction container storage portion 8. After that, the reaction container 9 into which the sample has been dispensed is gripped by the reaction container transfer device 11 and stored in a predetermined position of the reaction line 12. The reaction container 9 may be housed in the reaction table 12 and then the sample in the sample container 2 may be dispensed into the reaction container 9. The nozzle 5 of the sample dispensing apparatus 4 is cleaned by the nozzle cleaning unit 7 before or after the sample is dispensed.

The analysis operation will be described in more detail below. Of the plural standard substance solutions with different required concentrations, prepare one standard substance solution with the highest concentration, and use the diluent used to dilute this standard substance solution as the pressure transmitting substance in the tube. The sample dispensing device 4 used as the extruded water dispenses the sucked high-concentration reference substance solution into the empty reaction container 9 prepared in advance together with the extruded water. In this way, the dilution reference material solution is prepared in the sample container storage unit 1 of the automatic analyzer. Further, the diluted reference substance solutions of various concentrations are prepared by changing the dispensed amount of the diluting liquid depending on the suction amount of the high concentration reference substance solution and the extruded water. The concentration of this diluted standard substance solution is represented by C × a / (a + b). In this case, C: concentration of the high-concentration reference substance solution,
a: the amount of the high-concentration reference substance solution dispensed, b: the amount of the diluted solution dispensed. A calibration curve is created based on the relationship between the concentration values of the plurality of diluted substance solutions thus created and their measured values.

Here, the explanation will be given based on the sample container of FIG. 2. First, the number of reference substance solutions having different concentrations for preparing the calibration curve which is preset in the sample rack 3 is 1
Set empty sample containers 2-1 to 2-5. Continuously set the sample containers 2-0 containing the highest concentration reference material solution. Next, the amount of the reference substance solution having the highest concentration to be dispensed and the amount of the diluting liquid are calculated based on the concentration of the reference substance solution to be prepared by dilution, which is preset by an arithmetic device (not shown). Then, one of these standard substance solutions is dispensed by the sample dispensing device 4 into the empty sample container 2-1. In this case, the reference substance solution can be stirred by repeating suction and discharge of the sample dispensing device 4. This example shows an example in which the diluting liquid and the extruding water of the dispensing device are commonly used, but the present invention is not limited to this example, and the diluting liquid is separately prepared and the suction amount of the reference substance is separated. It is also possible to adopt a method in which the diluted solution is sucked and dispensed before and after the injection.

After the above-mentioned dispensing, the sample dispensing device 4 sequentially prepares the diluted standard substance solution according to the setting.
After preparing all diluted standard substance solutions, sample dispensing device 4
Dispense into the reaction container 9 of the reaction container storage portion 8 by. After that, the reaction container 9 in which the sample is dispensed is transferred to the reaction container transfer device 1
1 to transfer to the reaction table 12. The reaction container 9 transferred to the reaction table 12 and set therein is transferred along the reaction line.

With respect to the reaction vessel 9 thus transferred,
The first reagent dispensing device 19 is arranged from the first reagent container 17 corresponding to the analysis item set in the first reagent storage unit 15 to the first reagent container 17.
Dispense reagents. After dispensing, the nozzle 21 of the first reagent dispensing device 19 moves to the reagent nozzle cleaning unit 23 and is cleaned to prepare for the next reagent dispensing. After the first reagent is dispensed into the reaction container 9 of the reaction table 12, the stirrer 1 is added as necessary.
Stir according to 3.

Next, the second reagent dispensing device 20 is placed in the reaction container 9.
Dispenses the second reagent from the second reagent container 18 corresponding to the analysis item set in the second reagent storage section 16. After the dispensing, the nozzle 22 of the second reagent dispensing device 20 moves to the reagent nozzle cleaning unit 23 and is cleaned to prepare for the next dispensing.
It should be noted that stirring is performed by the stirring device 13 as needed, as in the above case. After that, the photometric device 14 measures the reaction amount of the test solution. In this way, all of the preset dilution standard material solutions are measured. Photometric device 1
The data measured by 4 is used to prepare an arithmetic expression that can be used as a calibration curve from the relationship with the concentration value preset by an arithmetic device (not shown).

In the method of preparing the dilution reference material solution described in the above embodiments, there is also a method of automatically setting and diluting the combination of the highest concentration reference material solution and the dilution liquid. That is, when the required number (n) of reference substance solutions is input, a combination is automatically set according to the following equation and a diluted reference substance solution is created. Maximum concentration reference substance solution: Diluent volume = [1 / n xk: (n-1) /
n × k, [2 / n × k: (n-2) / n × k. ． ． ． , [(N-1) /
n × k: 1 / n × k (k is a constant) When diluted in this way, the concentration value of the reference substance solution is n
It is possible to properly prepare a diluted reference substance solution that is evenly divided into individual pieces.

[0019]

As described above, according to the present invention, the analyzer can automatically prepare the diluted reference solutions having different concentrations by setting the highest concentration reference material solution and an empty container. In this respect, in comparison with the conventional method in which all the solutions of a plurality of diluting standard substances with different required concentrations had to be prepared by the inspector's hand and then automatically analyzed through the required steps, the diluting standard substance was compared. The operability of preparing the solution is improved, and since there is no manual operation of the inspector, the diluted reference substance solution can be homogenized and the accuracy is improved.

[Brief description of drawings]

FIG. 1 is a perspective view of an automatic analyzer used in the present invention.

FIG. 2 is a perspective view of a sample container rack.

[Explanation of reference symbols] 1 sample container storage part 2 sample container 3 sample container rack 4 sample dispensing device 5, 21, 22 nozzle 6 rail 7 reagent nozzle cleaning device 8 reaction container storage part 9 reaction container 10 for reaction container Rack 11 Reaction container transfer device 12 Reaction table 13 Stirring device 14 Photometric device 15 First reagent storage part 16 Second reagent storage part 17 First reagent container 18 Second reagent container 19 First reagent dispensing device 20 Second reagent Dispensing device 23 Reagent nozzle cleaning device

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[Procedure amendment]

[Submission date] January 8, 1992

[Procedure Amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0007

[Correction method] Change

[Correction content]

[0007]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view of an analyzer used in the automatic analysis method according to the present invention. A sample rack 3 holding a large number of sample containers 2 is detachably provided in the sample container storage unit 1. The sample rack 3 can be moved in the front-rear direction of the apparatus by a drive mechanism (not shown). There is. A sample dispensing device 4 for dispensing a sample is provided above the sample container storage portion 1, and its nozzle 5 can be moved horizontally and vertically via a rail 6. ing. Further, the nozzle 5 can be cleaned by a nozzle cleaning unit 7 provided in the horizontal movement direction. Since well-known moving mechanisms for the sample rack 3 and the sample dispensing device 4 are used, detailed description thereof will be omitted.

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0008

[Correction method] Change

[Correction content]

A reaction container storage portion 8 is provided near the sample storage portion 1, and a reaction container rack 10 holding a large number of reaction containers 9 is detachably provided in the reaction container storage portion 8. .. The reaction container rack 10 can be moved in the front-rear direction of the apparatus by a drive mechanism (not shown). Above the reaction container accommodating portion 8, there is provided a reaction container transfer device 11 for gripping and lifting the reaction container 9 and transferring it while moving horizontally via the rail 6. The reaction container storage section 8 is maintained at a constant temperature which is almost equal to that for a reaction table described later or a predetermined temperature for each item by a thermostat (not shown).

[Procedure 3]

[Document name to be amended] Statement

[Correction target item name] 0010

[Correction method] Change

[Correction content]

A first reagent storage portion 15 and a second reagent storage portion 16 are provided near the reaction table 12.
In this embodiment, the first reagent and the second reagent are set in their respective storages, but a large number of reagents such as the third reagent and the fourth reagent are set as needed. You can also A first reagent container 17 corresponding to an analysis item can be set in the first reagent storage unit 15, and a second reagent container 18 corresponding to an analysis item can be set in the second reagent storage unit 16. There is. Further, the first reagent container 17 and the second reagent container 18 can be moved in the front-rear direction of the device by a drive mechanism (not shown).

[Procedure amendment 4]

[Document name to be amended] Statement

[Correction target item name] 0013

[Correction method] Change

[Correction content]

The analysis operation will be described in more detail below. Of the plural standard substance solutions with different required concentrations, prepare one standard substance solution with the highest concentration, and use the diluent used to dilute this standard substance solution as the pressure transmitting substance in the tube. The sample dispenser 4 used as the extruded water dispenses the sucked high-concentration standard substance solution into the empty sample container 2 prepared in advance together with the extruded water. In this way, the dilution reference material solution is prepared in the sample container storage unit 1 of the automatic analyzer. Further, the diluted reference substance solutions of various concentrations are prepared by changing the dispensed amount of the diluting liquid depending on the suction amount of the high concentration reference substance solution and the extruded water. The concentration of this diluted standard substance solution is represented by C × a / (a + b). In this case, C: concentration of the high-concentration reference substance solution,
a: the amount of the high-concentration reference substance solution dispensed, b: the amount of the diluted solution dispensed. A calibration curve is created based on the relationship between the concentration values of the plurality of diluted substance solutions thus created and their measured values.

Claims (2)

[Claims] 1. In an automatic analysis method for preparing a calibration curve using a plurality of reference substances having different concentrations, a plurality of diluted reference substance solutions are prepared from the set reference substance solution and dilution water, and ordinary analysis is performed. After that, based on those measured values by analysis and the concentration value of the reference substance in the diluted reference substance solution stored in advance, an automatic calibration curve is created that represents the relationship between the measured value and the concentration value. Analysis method. 2. An automatic analyzer for preparing a calibration curve using a plurality of reference substances having different concentrations, is provided with a sample container rack having a sample container containing a reference substance solution and an empty sample container, and a diluent. An automatic analyzer characterized in that a sample dispensing device for dispensing a reference substance solution and a reference substance solution is provided to prepare a plurality of diluted reference substance solutions.