JPH0593675A - Dispenser - Google Patents
DispenserInfo
- Publication number
- JPH0593675A JPH0593675A JP28075991A JP28075991A JPH0593675A JP H0593675 A JPH0593675 A JP H0593675A JP 28075991 A JP28075991 A JP 28075991A JP 28075991 A JP28075991 A JP 28075991A JP H0593675 A JPH0593675 A JP H0593675A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- syringe
- dispensing
- suction
- stages
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は液状試料を分注する分注
装置に関し、特に試料を複数段階に希釈した希釈系列を
作成するのに好都合な分注装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dispenser for dispensing a liquid sample, and more particularly to a dispenser suitable for preparing a dilution series in which a sample is diluted in a plurality of stages.
【0002】[0002]
【従来の技術】分注装置は試料や試薬を吸引し吐出する
ために、シリンジとその駆動機構を備えている。分注装
置を用いて試料を希釈し、複数段階の濃度をもつ希釈系
列を作成するとき、試料と希釈液をそれぞれ独立して吸
引し、同一の試料容器に分注する。このとき、分注の方
法としては、次の2つの方法が考えられる。(1)シリ
ンジに一括して吸引し、指定量ずつ試料容器に分注する
方法、(2)シリンジに指定量を吸引し、その吸引した
液を一度に1つの試料容器に分注する方法。2. Description of the Related Art A dispensing device is equipped with a syringe and a driving mechanism for sucking and discharging a sample and a reagent. When a sample is diluted using a dispenser to create a dilution series having multiple levels of concentration, the sample and diluent are aspirated independently and dispensed into the same sample container. At this time, the following two methods can be considered as the dispensing method. (1) A method in which a syringe is aspirated all at once, and a designated amount is dispensed into a sample container, and (2) A designated amount is aspirated into a syringe and the aspirated liquid is dispensed into one sample container at a time.
【0003】[0003]
【発明が解決しようとする課題】希釈系列を作る場合
に、上記のいずれの方法によるときも分注の回数を数え
ながら分注操作を行なわなければならない。そのため、
途中で数え間違いが生じると試料が無駄になる。また、
吸引の回数と分注の回数を合わせた合計の操作回数が多
くなり、作業能率が悪い問題もある。本発明は試料の希
釈系列を容易に作成することのできる分注装置を提供す
ることを目的とするものである。When making a dilution series, it is necessary to carry out the dispensing operation while counting the number of dispensing in any of the above methods. for that reason,
If a counting error occurs on the way, the sample will be wasted. Also,
The total number of operations, including the number of times of suction and the number of times of dispensing, is increased, and there is a problem that work efficiency is poor. An object of the present invention is to provide a dispensing device that can easily prepare a dilution series of a sample.
【0004】[0004]
【課題を解決するための手段】図1により本発明を説明
する。段階数記憶部2は希釈系列の段階数Nを記憶し、
余分吸引量記憶部4はシリンジ12に吸引した液のうち
分注の際に吐出しないでシリンジ12に残す余分吸引量
vを記憶する。入力部6は段階数記憶部2に段階数Nを
記憶させ、余分吸引量記憶部4に余分吸引量vを記憶さ
せる。分注制御部8はシリンジの吸引量Vに対して(V
−v)を希釈系列の段階に応じて複数回に分けて吐出す
るとともに、表示部14に対して分注すべき順序を表示
させる。10は分注制御部8の指示を受けてシリンジ1
2の吸引動作と吐出動作を駆動するシリンジ駆動機構で
ある。The present invention will be described with reference to FIG. The stage number storage unit 2 stores the stage number N of the dilution series,
The extra suction amount storage unit 4 stores the extra suction amount v of the liquid sucked into the syringe 12, which is left in the syringe 12 without being discharged during dispensing. The input unit 6 stores the number N of stages in the number-of-stages storage unit 2 and the extra suction amount v in the excess suction amount storage unit 4. The dispensing control unit 8 sets (V
-V) is ejected in a plurality of times according to the stage of the dilution series, and the display unit 14 displays the order to be dispensed. 10 is a syringe 1 in response to an instruction from the dispensing control unit 8.
2 is a syringe drive mechanism that drives the suction operation and the discharge operation of No. 2.
【0005】[0005]
【作用】図2を参照して本発明の動作を説明する。試料
と希釈液を吸引するシリンジの最大吸引量をV、余分吸
引量をvとし、N段階の希釈系列を作るものとする。N
及びvを入力部6から入力し、段階数記憶部2、余分吸
引量記憶部4にそれぞれ登録する。0,1/N,2/
N,……M/N,……N/Nの希釈系列を効果的に作る
のが本発明の骨子である。最初に分注ノズルの先を試料
に当て、Vの試料をシリンジに吸引する。試料Vのうち
vは希釈系列の作成が終わるまで保持したままで(V−
v)を何段階かに分けて分注する。一番効果的なのは、
1回の吸引ごとに(V−v)を二度に分けて分注する方
法である。The operation of the present invention will be described with reference to FIG. Let V be the maximum suction amount of the syringe that aspirates the sample and the diluent, and let v be the excess suction amount, and create an N-stage dilution series. N
And v are input from the input unit 6 and registered in the stage number storage unit 2 and the extra suction amount storage unit 4, respectively. 0, 1 / N, 2 /
It is the essence of the present invention to effectively make a dilution series of N, ... M / N, ... N / N. First, the tip of the dispensing nozzle is applied to the sample, and the sample of V is sucked into the syringe. In the sample V, v is kept (V-
Dispense v) in several stages. The most effective is
This is a method in which (Vv) is divided and dispensed twice for each suction.
【0006】分注の方法は次の二種類がある。 (1)1回目の吸引のときは(V−v)/(N+1)と(V
−v)N/(N+1)に分けて1番目とN番目の試料容器
にそれぞれ分注する。2回目の吸引のときは(V−v)2
/(N+1)と(V−v)(N−1)/(N+1)に分けて2番
目と(N−1)番目の試料容器にそれぞれ分注する。M回
目の吸引のときは(V−v)M/(N+1)と(V−v)(N
−M+1)/(N+1)に分けてM番目と(N−M+1)番
目の試料容器にそれぞれ分注する。これをM≦(N−
M)、すなわちM≦N/2まで繰り返す。There are the following two types of dispensing methods. (1) (V-v) / (N + 1) and (V
-V) Divide into N / (N + 1) and dispense into the 1st and Nth sample containers, respectively. (Vv) 2 for the second suction
/ (N + 1) and (Vv) (N-1) / (N + 1) are divided and dispensed into the second and (N-1) th sample containers, respectively. During the Mth suction, (V-v) M / (N + 1) and (V-v) (N
Divide into -M + 1) / (N + 1) and dispense into the M-th and (N-M + 1) -th sample containers, respectively. This is M ≦ (N−
M), that is, M ≦ N / 2 is repeated.
【0007】(2)1回目の吸引のときは(V−v)/N
と(V−v)(N−1)/Nに分けて1番目と(N−1)番目
の試料容器にそれぞれ分注する。2回目の吸引のときは
(V−v)2/Nと(V−v)(N−2)/Nに分けて2番目
と(N−1)番目の試料容器にそれぞれ分注する。M回目
の吸引のときは(V−v)M/Nと(V−v)(N−M)/N
に分けてM番目と(N−M)番目の試料容器にそれぞれ分
注する。これをM≦(N−M)、すなわちM≦N/2ま
で繰り返す。試料と希釈液についてそれぞれ以上の操作
を行なう。ただし、試料を分注する試料容器と希釈液を
分注する試料容器は順序が全く逆になる。(2) At the first suction, (Vv) / N
And (Vv) (N-1) / N and dispensed into the first and (N-1) th sample containers, respectively. For the second suction
Divide into (Vv) 2 / N and (Vv) (N-2) / N and dispense into the second and (N-1) th sample containers, respectively. (V-v) M / N and (V-v) (NM) / N at the Mth suction
Dispense into the M-th and (NM) -th sample containers respectively. This is repeated until M ≦ (N−M), that is, M ≦ N / 2. Perform the above operations for the sample and diluent. However, the order of the sample container for dispensing the sample and the sample container for dispensing the diluting liquid is completely opposite.
【0008】これを具体的な例として10段階希釈系列
を作成する場合について考える。(1)の方法によれ
ば、1と(10)、2と9、3と8、4と7、5と6の
分注を行なうための5回の吸引を行なうことになる。
(2)の方法によれば、1と9、2と8、3と7、4と
6、5、(10)の分注を行なうための6回の吸引を行
なうことになる。また、5段階希釈系列を作る場合は、
(1)の方法によれば、1と(5)、2と4、3の分注
を行なうための3回の吸引を行なうことになる。(2)
の方法によれば、1と4、2と3、(5)の分注を行な
うための3回の吸引を行なうことになる。A case where a 10-step dilution series is prepared will be considered as a concrete example. According to the method (1), suction is performed 5 times for dispensing 1 and (10), 2 and 9, 3 and 8, 4 and 7, 5 and 6.
According to the method (2), suction is performed 6 times for performing the dispensing of 1 and 9, 2 and 8, 3 and 7, 4 and 6, 5, (10). Also, when making a 5-step dilution series,
According to the method (1), suction is performed three times for dispensing 1 and (5), 2 and 4, and 3. (2)
According to the method (1), suction is performed three times for performing the dispensing of 1, 4, 2, 3 and (5).
【0009】結局、希釈系列の段階数が偶数のときには
(1)の方が1回分少なくてすみ、奇数のときには同じ
回数になる。ただし、括弧つきで示した(10),
(5)を特に正確に分注する必要がなければ、偶数のと
きには同じ回数で、奇数のときには(2)の方が1回少
なくてすむ。Nの入力時に(1),(2)のいずれかを
選択できるように設定しておいてもよい。試料又は希釈
液を吸引するときは「吸引せよ」という内容のメッセー
ジが表示され、分注するときは分注すべき系列番号が表
示されるので、その表示された試料容器に分注を行な
う。After all, when the number of stages of the dilution series is even, (1) requires less one time, and when it is odd, the number is the same. However, parenthesized (10),
If it is not necessary to dispense (5) particularly accurately, the number of times is the same when the number is even and the number of times (2) is less when the number is odd. It may be set so that either (1) or (2) can be selected when N is input. When aspirating the sample or the diluting liquid, a message of "aspirate" is displayed, and when dispensing, the sequence number to be dispensed is displayed. Therefore, the dispensed sample container is dispensed.
【0010】1回の吸引について2回の吐出が行なわれ
ると、次の試料の吸引のためにノズルの先端を試料に当
て、吸引を行なう。シリンジにはvの試料が残っている
ので、2回目以降の吸引量は(V−v)である。最後の
吐出が終わると、余分吸引量vの試料を洗浄液で洗い出
す。希釈液については分注すべき系列番号の表示が試料
の場合とは逆になるので、最初に試料を分注するのか希
釈液を分注するのかを手動で切り換えるようにするか、
又は分注されるのが試料であるか希釈液であるかを自動
的に表示させるようにしてもよい。希釈系列のうち試料
が0の場合と希釈液が0の場合(試料のみの場合)は、
希釈液と試料は充分にあると考えられるので、この二種
類は特に希釈系列として作る必要はない。充分にない場
合の対策として、Nvを入力したときに一試料当りに必
要な総量を表示してもよい。また、以上の操作を複数回
繰り返せば、V−v以上の量の段階希釈系列を作ること
もできる。When the discharge is performed twice for one suction, the tip of the nozzle is brought into contact with the sample for suction of the next sample and suction is performed. Since the v sample remains in the syringe, the suction amount after the second time is (Vv). When the last discharge is completed, the extra suction amount v of the sample is washed out with the cleaning liquid. Since the display of the serial number to be dispensed for the diluting liquid is the reverse of that for the sample, whether to manually switch between dispensing the sample first and diluting liquid, or
Alternatively, it may be automatically displayed whether the sample or the diluted solution is dispensed. When the sample is 0 in the dilution series and when the diluent is 0 (only the sample),
Since it is considered that there are sufficient diluent and sample, these two types do not need to be prepared as a dilution series. As a countermeasure when there is not enough, the total amount required for one sample may be displayed when Nv is input. Further, by repeating the above operation a plurality of times, it is possible to prepare a serial dilution series having an amount of Vv or more.
【0011】[0011]
【実施例】図3は本発明が適用される分注装置の機構部
の一例を表わしている。20は試料や希釈液を吸引し吐
出するノズル、22は試料の吸引と吐出のためのシリン
ジ、24は希釈液の吸引と吐出のためのシリンジであ
る。シリンジ22はノズル20に直接接続され、シリン
ジ24は三方電磁弁26を介してノズル20に接続され
ている。三方電磁弁26にはさらに洗浄液28の流路が
接続されている。電磁弁26は実線で示された方向が接
続状態になる。ノズル20の先端からシリンジ22まで
の流路の容積はシリンジ22の最大吸引量Vよりも小さ
くしておく。DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 3 shows an example of a mechanical portion of a dispensing device to which the present invention is applied. Reference numeral 20 is a nozzle for sucking and discharging the sample and the diluent, 22 is a syringe for sucking and discharging the sample, and 24 is a syringe for sucking and discharging the diluent. The syringe 22 is directly connected to the nozzle 20, and the syringe 24 is connected to the nozzle 20 via a three-way electromagnetic valve 26. A flow path for the cleaning liquid 28 is further connected to the three-way solenoid valve 26. The solenoid valve 26 is connected in the direction indicated by the solid line. The volume of the flow path from the tip of the nozzle 20 to the syringe 22 is set smaller than the maximum suction amount V of the syringe 22.
【0012】シリンジ24を用いて希釈系列を作成する
場合を説明する。試料と希釈液の分注を行なうときは、
図4(A)と(B)に示されるように、三方電磁弁26
をノズル20とシリンジ24を接続する方向に設定して
おく。シリンジ24により試料を吸引し、希釈系列の段
階に応じて(V−v)の試料を対応する2つの比率に分
割してそれぞれの試料容器に分注する。各段階の試料の
分注が終わると、次に同様にして希釈液を分注する。A case where a dilution series is created using the syringe 24 will be described. When dispensing the sample and diluent,
As shown in FIGS. 4A and 4B, the three-way solenoid valve 26
Is set in the direction in which the nozzle 20 and the syringe 24 are connected. The sample is aspirated by the syringe 24, and the (Vv) sample is divided into two corresponding ratios according to the stages of the dilution series and dispensed into the respective sample containers. After the dispensing of the sample at each stage is completed, the diluent is dispensed in the same manner.
【0013】洗浄を行なうときは、図4(C)に示され
るように三方電磁弁26をシリンジ24と洗浄液28を
接続する方向に切り換えて洗浄液を吸引し、次に(D)
に示されるように三方電磁弁26をシリンジ24とノズ
ル20を接続する方向に切り換えて洗浄液を吐出する。
この洗浄液の吸引と吐出の動作を繰り返す。分注量が少
ないときにはシリンジ22を使って試料と希釈液の分注
を上記と同様に行なうようにし、洗浄はシリンジ24で
行なうようにしてもよい。When cleaning is performed, as shown in FIG. 4C, the three-way solenoid valve 26 is switched to the direction in which the syringe 24 and the cleaning liquid 28 are connected to suck the cleaning liquid, and then (D).
As shown in FIG. 3, the three-way solenoid valve 26 is switched to connect the syringe 24 and the nozzle 20 to discharge the cleaning liquid.
The operations of sucking and discharging the cleaning liquid are repeated. When the dispensed amount is small, the syringe 22 may be used to dispense the sample and the diluent in the same manner as described above, and the washing may be performed using the syringe 24.
【0014】[0014]
【発明の効果】本発明では試料を分注する際にシリンジ
に余分吸引量vを残すようにしたので、試料が洗浄液で
希釈されるのを防ぐことができる。1回の吸引量を複数
回に分けて分注するので、希釈系列を効率的に、かつ容
易に作成することができる。In the present invention, the extra suction amount v is left in the syringe when the sample is dispensed, so that the sample can be prevented from being diluted with the washing solution. Since a single suction amount is divided into a plurality of portions and dispensed, a dilution series can be efficiently and easily created.
【図1】本発明を示すブロック図である。FIG. 1 is a block diagram showing the present invention.
【図2】動作の一例を示すフローチャート図である。FIG. 2 is a flowchart showing an example of operation.
【図3】本発明が適用される分注装置を示す概略構成図
である。FIG. 3 is a schematic configuration diagram showing a dispensing device to which the present invention is applied.
【図4】同実施例の動作を示す図である。FIG. 4 is a diagram showing an operation of the example.
2 段階数記憶部 4 余分吸引量記憶部 6 入力部 8 分注制御部 10 シリンジ駆動機構 12 シリンジ 14 表示部 2 step number storage unit 4 extra suction amount storage unit 6 input unit 8 dispensing control unit 10 syringe drive mechanism 12 syringe 14 display unit
Claims (1)
憶部、シリンジに吸引した液のうち分注の際に吐出しな
いでシリンジに残す余分吸引量vを記憶する余分吸引量
記憶部と、段階数記憶部に段階数Nを記憶させ、余分吸
引量記憶部に余分吸引量vを記憶させる入力部と、シリ
ンジの吸引量Vに対して(V−v)を希釈系列の段階に
応じて複数回に分けて吐出するとともに、表示部に対し
て分注すべき順序を表示させる分注制御部とを備えた分
注装置。1. A number-of-stages storage unit that stores the number of stages N of the dilution series, and an extra-aspiration amount storage unit that stores an extra-aspiration amount v of the liquid aspirated in the syringe, which is left in the syringe without being discharged during dispensing. , (V-v) for the suction amount V of the syringe and the input unit for storing the number N of stages in the number-of-stages storage unit and the extra suction amount v in the excess suction amount storage unit, depending on the stage of the dilution series And a dispensing control unit that displays the order to be dispensed on the display unit.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP28075991A JPH0593675A (en) | 1991-09-30 | 1991-09-30 | Dispenser |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP28075991A JPH0593675A (en) | 1991-09-30 | 1991-09-30 | Dispenser |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH0593675A true JPH0593675A (en) | 1993-04-16 |
Family
ID=17629561
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP28075991A Pending JPH0593675A (en) | 1991-09-30 | 1991-09-30 | Dispenser |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0593675A (en) |
-
1991
- 1991-09-30 JP JP28075991A patent/JPH0593675A/en active Pending
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