JPH0585925A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having excellent moisture retention free from greasiness resulting from a water-soluble polyhydric alcohol by blending a water-soluble polyhydric alcohol with a specific polysaccharide. CONSTITUTION:A cosmetic comprising (a) a water-soluble polyhydric alcohol such as dipropylene glycol, polyglycerol or reducing saccharide and (B) a polysaccharide produced by a Gram-negative aerobic bacterium such as Alcaligenes latus B-16 strain (FERM-BP-2,015), capable of producing a polysaccharide, in a ratio of 3-40wt.% component A and 0.001-5.0wt.%, preferably 0.01-1.0wt.% component B based on the total amount of the final composition. The polysaccharide consists essentially of rhamnose, fucose, glucose, mannose and glucuronic acid, its constitution ratio by molecular ratio is (1-10):(2-10):(4-20):1-(1-5), the ratio of elemental analysis (wt.%) is C:40+ or -4, H:6+ or -1.0, O:54+ or -5, a carbonization temperature is 225-280 deg.C and solubility is slightly soluble in water, soluble in alkali and insoluble in methanol, ethanol and acetone.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition which is excellent in moisturizing property as well as solving tackiness caused by water-soluble polyhydric alcohol by blending a specific polysaccharide.

[0002]

2. Description of the Related Art In order to keep the skin healthy, it is important to keep the water content of the skin appropriately. For this purpose, many cosmetics, quasi drugs, and pharmaceuticals are used. It is on sale. Studies on moisturizing are also actively conducted, and NMF (natural moisturizing factor) such as water-soluble polyhydric alcohols represented by glycerin and sorbitol, hyaluronic acid, water-soluble polymers represented by xanthene gum, amino acids, pyrrolidonecarboxylic acid, etc. ) Is used as a moisturizer in cosmetics, quasi drugs, and pharmaceuticals. Among them, water-soluble polyhydric alcohols and water-soluble polymers are widely used because of their high moisturizing effect, and are often used together when a high moisturizing effect is expected.

However, water-soluble polyhydric alcohols and water-soluble polymers have an excellent moisturizing effect, but on the other hand, they have a sticky feeling at the time of use, and particularly when both are used together, it becomes remarkable, and the sensory properties are emphasized. So it was a big problem.

Japanese Examined Patent Publication (Kokoku) No. 64-10483 proposes to eliminate the stickiness in use caused by the water-soluble polyhydric alcohol by blending a polyoxyalkylene alkyl glycoside ether. Although it is effective when only water-soluble polyhydric alcohol is used as a moisturizer, it is not sufficient in a system that requires a high moisturizing effect, such as using water-soluble polyhydric alcohol and water-soluble polymer.

[0005]

[Means for Solving the Problems] As a result of intensive studies in view of such circumstances, it was found that by combining water-soluble polyhydric alcohol and a specific polysaccharide together, there is no sticky feeling, and each is higher than when used alone. Cosmetics with moisturizing effect,
The inventors have found that they can be obtained and have completed the present invention.

The polysaccharides used in the present invention have a moisturizing effect when used alone, but when incorporated into cosmetics, the viscosity becomes too high, and it is difficult to add an amount commensurate with a sufficient moisturizing effect. .. Therefore, it was found that when used in combination with a polyhydric alcohol, not only a sufficient effect can be expected even at a relatively low concentration due to a synergistic effect, but also a sticky feeling due to the polyhydric alcohol is reduced.

That is, the present invention is characterized in that a polysaccharide having 3 to 40% by weight of a water-soluble polyhydric alcohol having two or more hydroxyl groups in the molecule is blended with a polysaccharide having the following properties. And the cosmetics.

(1) Sugar composition by thin layer chromatography, liquid chromatography, gas chromatography; consisting of major constituents of rhamnose, fucose, glucose, mannose and glucuronic acid, and the constituent ratio of each of these constituents is molar. Each in ratio (1-10):
(2 to 10): (4 to 20): (1): (1 to 5), (2) Elemental analysis ratio (wt%) C: 40 ± 4 H: 6 ± 1 O: 54 ± 5 (3) Carbonization point 225 to 280 ° C (4) Solubility Insoluble in water (neutral); Soluble in alkali; Insoluble in methanol, ethanol, and acetone (5) Ultraviolet absorption spectrum Unique to protein (peptide), 280 nm, and nucleic acid an absorption of 260nm is not recognized, (6) infrared absorption spectrum ^{800~1200cm -1, 1620 ± 20cm -1,} 29
It has peaks at 50 ± 10 cm ⁻¹ and 3400 ± 20 cm ⁻¹ . Preferably, the polysaccharide contains glucuronic acid in an amount of 5 to 25 mol%, and the composition ratio of each component of rhamnose, fucose, glucose and mannose is (1 to 6) :( 3 to
5): (5-17): 1 acidic polysaccharide. More preferably, the polysaccharide is rhamnose, fucose, glucose,
The constituent ratio of each component of mannose and glucuronic acid is (1-3) :( 3-5) :( 5-7): 1: (2
It is the acidic polysaccharide of 3).

The present invention can also be applied to dosage forms such as creams, emulsions, lotions, emulsion foundations and sunscreens.

The water-soluble polyhydric alcohol used in the present invention has two or more hydroxyl groups in the molecule, and examples of those having two hydroxyl groups include dipropylene glycol and 1,3 butylene glycol. Examples of compounds having 3 or more hydroxyl groups in the molecule include polyglycerin such as glycerin, diglycerin, triglycerin, tetraglycerin, pentaglycerin, hexaglycerin, maltose, maltitol, sucrose, fructose, xylose. , Sorbitol, threitol,
Examples include reducing sugars such as erythritol. In the cosmetic of the present invention, one or two of these water-soluble polyhydric alcohols are used.
It can be used as a mixture of two or more species.

The present invention is particularly effective for a cosmetic composition containing a water-soluble polyhydric alcohol having three or more hydroxyl groups rather than one having two hydroxyl groups in the molecule.

The amount of the water-soluble polyhydric alcohol used in the present invention is 3 to 40% by weight based on the total amount of the final composition.
Is. If the blending amount is less than 3% by weight, there is no sticky feeling, but a sufficient moisturizing effect cannot be obtained. 40% by weight
If the concentration exceeds, the sticky feeling cannot be suppressed and the moisturizing effect corresponding to the blending amount cannot be obtained.

The polysaccharide used in the present invention is described in JP-A-2-
It is a polysaccharide produced by the microorganism described in Japanese Patent No. 291292 and has the above-mentioned properties. The polysaccharide-producing microorganism of the present invention is a gram-negative aerobic bacterium belonging to the genus Alcaligenes, and a representative exemplified strain thereof is Alcaligenes latus B-16.
The strain has been deposited as FERM BP-2015. Carbon sources include fructose, glucose, monosaccharides and oligosaccharides such as sucrose, hemicellulose,
Natural polymers such as starch and corn starch and oils such as olive oil are preferably used. In addition to the carbon source, an organic nitrogen source and inorganic salts are used as medium constituents, and liquid culture is preferable for the culture, with an initial pH of 4-10 and a temperature of 15-4.
It is carried out at 0 ° C., usually by aeration and stirring culture.

The content of the polysaccharide is appropriately 0.001 to 5.0% by weight, preferably 0.01 to 1.0% by weight, based on the total amount of the final composition. The compounding amount is 0.
If it is less than 001% by weight, the sticky feeling due to the water-soluble polyhydric alcohol cannot be sufficiently prevented,
In addition, a sufficient moisturizing effect may not be obtained in some cases. If the blending amount exceeds 5.0% by weight, the viscosity becomes high and the formulation is limited.

In the cosmetic of the present invention, in addition to the above-mentioned essential components, a fragrance, a coloring agent, an antiseptic, an oily base, an aqueous base, and a pigment may be added, if necessary, within the range to achieve the object of the present invention. , UV absorbers, sequestering agents for metal salts, alcohols, medicinal ingredients, plant extracts and the like, which are usually used in cosmetics, can be added.

[0016]

EXAMPLES The present invention will be described below with reference to Examples and Comparative Examples. In the examples,% means% by weight. The present invention is not limited to the examples given here.

The moisturizing test and sensory test in the examples were carried out as follows. (Humidity test) A 20 ml vial bottle with a diameter of 1 cm was filled with 10 ml of water, the mouth was covered with paper, and the test substance of 0.
1 g is evenly applied. This was put in a desiccator with calcium chloride, this desiccator was put in a constant temperature layer at 30 ° C., and the moisture retention was calculated from the following formula as the weight change of the vial after leaving the amount of water evaporated for 24 hours and 48 hours. Moisture retention (%) = {10- (weight after standing for 48 hours-24)
(Weight after leaving for an hour)} / 10 × 100

(Sensory test) Practical tests were conducted by 20 panelists, and the number of panelists who answered "feels sticky" and "has a sufficient moisturizing effect" is shown.

Experimental Example 1 (Preparation of Polysaccharide Used in the Present Invention) Sucrose 15 g, KH ₂ PO ₄ 6.8 g, K ₂ H
PO4 8.8 g, dissolved MgSO ₄ · 7H ₂ O 0.2g, salt 0.1 g, urea 0.5g, meat extract 0.5g distilled water 1l, medium was adjusted to pH 7.4. Medium 15
0 ml was put in a 500 ml Erlenmeyer flask and sterilized by autoclave at 102 ° C. for 15 minutes, and then Alcaligenes lettus B-16 strain (FERM BP-
No. 2015) was transplanted into the flask in an amount of 1 platinum loop, and
Rotary culture is performed at ℃. Rotation speed is 180 rp
m.

The polysaccharide used in the present invention is purified from the culture (bacteria-containing body) on day 6 of culture by the following method.

That is, twice the amount of ethanol was added to 500 ml of the culture and the mixture was allowed to stand still, and then the liquid phase part was removed to collect the precipitate. 100 ml of pure was added to this precipitate, and 60
Dissolve in a water bath at ˜70 ° C., then add 5 volumes of ethanol to obtain a precipitate again. By repeating this dissolution-precipitation operation several times, the coloring substances that are considered to be derived from the medium components are excluded from the system, and a white precipitate is obtained. The white precipitate was diluted again with 0.02-8000 ml of 0.02% NaOH solution and redissolved at 121 ° C and 10 ° C.
After heating for 4 minutes, use a centrifuge for 40000 x 40 minutes,
Dilute and centrifuge. The cells are removed by this dilution centrifugation operation. The centrifugation supernatant portion from which the bacterial cells have been removed is neutralized with hydrochloric acid, and concentrated using a rotary evaporator (60 to 70 ° C). Add 100 ml of pure water to this concentrate,
Re-dissolve in the hot water bath. To the redissolved solution, 5 times the amount of ethanol is added to cause ethanol precipitation. After repeating this dissolution-ethanol precipitation three times, vacuum drying at normal temperature gives a white purified polysaccharide.

In addition,

[0019]

The polysaccharide obtained in (1) was used as Diaion HPA-
75 (OH ⁻ ) (Nippon Rensui Co., Ltd.) 1000 ml column is treated at 8 Rv (8 times the resin volume) or less. Here, proteins, nucleic acids and low molecular weight components are removed. next,
A filter aid RL700 (Radiolite) +5 μm membrane filter is applied to remove bacteria. The solution after sterilization is HCl
The solution is neutralized to pH 7 and concentrated, and the precipitate is collected by doubling the amount of acetone. After washing 5 times with 10 times amount of acetone, vacuum drying at room temperature gives a white purified polymer component polymer having a molecular weight of 1 × 10 ⁶ or more of the polysaccharide by liquid chromatography. The high-molecular component polymer obtained here is

Like the polysaccharide obtained in the above, it can be preferably used for the purpose related to the present invention.

Example 1 (Lotion) (Composition) (wt%) 1. Ethanol 10.0 2. POE (60) hydrogenated castor oil 2.0 3. Methylparaben 0.1 4. Fragrance 0.05 5. Glycerin 10.0 6. Purified polysaccharide obtained in Example 1 (former) 0.15 7. Purified water 77.7 (Production method) (1) 1 to 4 are uniformly mixed and dissolved. (2) Disperse 5 to 7 uniformly. (2) was added to (1) and uniformly mixed and stirred to obtain a lotion of the present invention.

Comparative Example 1 (Lotion) (Composition) (wt%) 1. Ethanol 10.0 2. POE (60) hydrogenated castor oil 2.0 3. Methylparaben 0.1 4. Fragrance 0.05 5. Glycerin 10.0 6. Xanthene gum 0.15 7. Purified water 77.7 (Production method) (1) 1 to 4 are uniformly mixed and dissolved. (2) Disperse 5 to 7 uniformly. (2) was added to (1) and uniformly mixed and stirred to obtain a lotion for comparison.

Comparative Example 2 (Lotion) (Composition) (wt%) 1. Ethanol 10.0 2. POE (60) hydrogenated castor oil 2.0 3. Methylparaben 0.1 4. Fragrance 0.05 5. Glycerin 10.0 6. Purified water 77.85 (Production method) (1) 1 to 4 are uniformly mixed and dissolved. (2) Disperse 5 and 6 uniformly. (2) was added to (1) and uniformly mixed and stirred to obtain a lotion for comparison.

Comparative Example 3 (Lotion) (Composition) (wt%) 1. Ethanol 10.0 2. POE (60) hydrogenated castor oil 2.0 3. Methylparaben 0.1 4. Fragrance 0.05 5. 5. Purified polysaccharide obtained in Example 1 (former) 0.15 6. Purified water 87.7 (Production method) (1) 1 to 4 are uniformly mixed and dissolved. (2) Disperse 5 and 6 uniformly. (2) was added to (1) and uniformly mixed and stirred to obtain a lotion for comparison.

The moisturizing test and sensory test described above were carried out on the lotion of the present invention and the lotion for comparison. The results are shown in Table 1.

[0028]

[Table 1]

As shown in Table 1, the lotion of the present invention was superior to the comparative lotion in the moisturizing test and the sensory test. Comparative Example 1 using xanthene gum instead of the purified polysaccharide obtained in Experimental Example 1 showed the same effect as the lotion of the present invention in the item of "moisturizing effect" of the moisturizing test and the sensory test. In the sensory test “stickiness” item, more than half of the panelists answered that they felt “stickiness”, which was the worst among the compared samples. Comparative Example 2 in which only a water-soluble polyhydric alcohol was blended as a moisturizer was not preferable because a sufficient moisturizing effect was not obtained in the moisturizing test and the sensory test, and a large number of people answered that "a sticky feeling was felt". .. Comparative Example 3 in which only the purified polysaccharide (the former) obtained in Experimental Example 1 was blended as a moisturizer was preferable because the number of respondents who "feel a sticky feeling" was small and the moisturizing property was poor.

Example 2 (Cream) (Composition) (wt%) 1. Stearic acid 3.0 2. Cetanol 2.0 3. Squalane 6.0 4. Lanolin 1.5 5. Isocetyl myristate 5.0 6. Glycerin monostearate 3.0 7. Methylparaben 0.1 8. Sorbitol 15.0 9. Purified polysaccharide obtained in Experimental Example 1 (former) 0.8 10. Sodium cetyl sulfate 1.0 11. Purified water 63.2 (Production method) (1) Heat 1 to 6 at 80 ° C to completely dissolve them. (2) After uniformly dispersing 7 to 11, the mixture is heated to 80 ° C. After adding (2) to (1) and emulsifying, it was cooled to room temperature to obtain the cream of the present invention.

Example 3 (Emulsion) (Composition) (wt%) 1. Stearic acid 0.5 2. Cetanol 1.0 3. Squalane 3.5 4. POE (20) sorbitan oleate 1.5 5. Macadamia nut oil 5.0 6. Glycerin monostearate 2.0 7. Methylparaben 0.5 8. Dipropylene glycol 5.0 9. Purified polysaccharide obtained in Experimental Example 1 (former) 0.2 10. Sodium acylglutamate 1.0 11. Purified water 79.8 (manufacturing method) (1) 1-6 are heated at 80 degreeC, and it melt | dissolves completely. (2) After uniformly dispersing 7 to 11, the mixture is heated to 80 ° C. After adding (2) to (1) and emulsifying, it was cooled to room temperature to obtain the emulsion of the present invention.

Example 4 (Emulsion foundation) (Composition) (wt%) 1. Stearic acid 2.0 2. Cetanol 3.5 3. Squalane 8.0 4. POE (20) sorbitan oleate 0.5 5. Vaseline 2.0 6. Glycerin monoisostearate 2.5 7. Methylparaben 0.5 8. Hexaglycerin 5.0 9. Sorbitol 10.0 10. Purified polysaccharide obtained in Experimental Example 1 (former) 0.2 11. Sodium cetyl sulfate 1.0 12. Formulated powder 15.0 13. Purified water 59.8 (Manufacturing method) (1) 1-6 are heated at 80 degreeC, and it melt | dissolves completely. (2) After uniformly dispersing 7 to 13, the mixture is heated to 80 ° C. After adding (2) to (1) and emulsifying, it was cooled to room temperature to obtain the emulsified foundation of the present invention.

Example 5 (Soft lotion) (Composition) (wt%) 1. Ethanol 10.0 2. POE (60) hydrogenated castor oil 2.0 3. Methylparaben 0.1 4. Fragrance 0.05 5. Maltitol 5.0 6. Glycerin 10.0 7. Purified polysaccharide obtained in Experimental Example 1 (former) 0.03 8. Sodium carbonate 0.01 9. Sodium hydrogen carbonate 0.05 10. Purified water 72.76 (Production method) (1) 1 to 4 are uniformly mixed and dissolved. (2) Disperse 5 to 10 uniformly. (2) was added to (1) and uniformly mixed and stirred to obtain a lotion of the present invention.

Example 6 (Cream) (Composition) (wt%) 1. Stearic acid 3.0 2. Cetanol 2.0 3. Squalane 6.0 4. Lanolin 1.5 5. Isocetyl myristate 5.0 6. Glycerin monostearate 3.0 7. Methylparaben 0.1 8. Sorbitol 15.0 9. Glycerin 20.0 10. Purified polysaccharide obtained in Experimental Example 1 (former) 0.8 11. Sodium cetyl sulfate 1.0 12. Purified water 43.2 (Production method) (1) Warm 1 to 6 at 80 ° C to completely dissolve it. (2) After uniformly dispersing 7 to 12, the mixture is heated to 80 ° C. After adding (2) to (1) and emulsifying, it was cooled to room temperature to obtain the cream of the present invention.

The cosmetics of the present invention of Examples 2 to 6 were as excellent as those of Example 1 in the moisturizing test and the sensory test.

[0036]

As described above, according to the present invention, by combining the water-soluble polyhydric alcohol and the specific polysaccharide together, the stickiness of the feeling of use due to the water-soluble polyhydric alcohol is eliminated and the moisturizing effect is maintained. It is obvious to provide a cosmetic having excellent properties.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Ryuichiro Kurane 1-3-1, Higashi Tsukuba, Ibaraki Prefecture Institute of Industrial Technology, Institute of Microbial Science and Technology (72) Inventor Masakatsu Ota 5-3 Kotoba, Odawara-shi, Kanagawa −28 Kanebo Co., Ltd. In the Cosmetic Research Laboratory (72) Inventor Yasuhiro Nobata Yokkaichi City, Mie Prefecture 6-6-9 Hakuto Co., Ltd. In the Yokkaichi Research Center

Claims (2)

[Claims] 1. A cosmetic comprising 3 to 40% by weight of a water-soluble polyhydric alcohol having two or more hydroxyl groups in the molecule, wherein a polysaccharide having the following properties is blended. Materials: (1) Sugar composition by thin layer chromatography, liquid chromatography, gas chromatography; composed of major constituents of rhamnose, fucose, glucose, mannose and glucuronic acid, and the constituent ratio of each of these constituents is molar ratio. And each (1-10): (2-10):
(4 to 20): (1): (1 to 5), (2) Elemental analysis ratio (wt%) C: 40 ± 4 H: 6 ± 1 O: 54 ± 5 (3) Carbonization point 225 to 280 ° C (4) Solubility Insoluble in water (neutral); Soluble in alkali; Insoluble in methanol, ethanol, and acetone (5) Ultraviolet absorption spectrum Absorption at 280 nm peculiar to proteins (peptides) and 260 nm peculiar to nucleic acids is recognized. Never, (6) infrared absorption spectrum ^{800~1200cm -1, 1620 ± 20cm -1,} 29
It has peaks at 50 ± 10 cm ⁻¹ and 3400 ± 20 cm ⁻¹ . 2. The polysaccharide is Alcaligenes (Alcali).
gene) bacterial culture or a treated product thereof.