JPH03167109A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having low skin irritation, excellent functional characteristics, especially slight feeling of greasiness by blending a cosmetic base with a specific beta-glucan produced by culture of a fungus belonging to the genus Macrophomopsis. CONSTITUTION:A cosmetic blended with a high-viscosity beta-glucan produced by culture of a fungus belonging to the genus Macrophomopsis. beta-Glucan has properties wherein D-glucopyranose residue of main chain wholly has beta-1,3 bond, branches at both the C-2 and C-6 positions and D-glucopyranose residue of side chain does not have beta-1,4 bond.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a cosmetic composition that is non-irritating to the skin, has excellent organoleptic properties, and particularly has a low sticky feeling. [Improving the sensory properties of cosmetics] Various thickeners (gelling agents) have been used to improve the sensory properties of cosmetics.
For example, as a water-soluble thickener,
Begum.

Xanthan gum, pectin, casiginan, carboxyvinyl polymer salts, etc. are commonly used, and as oil-soluble thickeners, organic bentonite, sucrose fatty acid ester 6-dextrin fatty acid ester, etc. are known.

Cosmetics containing these thickeners have drawbacks in terms of organoleptic properties, such as (a) "spreadiness" (stretchability) and (b) "sticky feeling."

An object of the present invention is to provide a cosmetic composition that is not irritating to the skin and has excellent organoleptic properties, particularly in terms of being less sticky and confusing.

[! ! Means to solve the problem]

The present invention relates to macrophomopsis (Macrophomopsis).
This cosmetic is produced by culturing microorganisms belonging to the genus Sis, and is characterized by containing β-glucan having the structure of the binding mode described below.

Note (8) All D-glucopyranose residues in the main chain are β
-1,3 bond, (1)) C-2 of the D-glucopyranose residue in the main chain
and/or branched at the C-6 position, and the (c) side chain D-glucopyranose residue has β1
．． There are no 4 bonds.

The β-glucan used in the present invention is disclosed in Japanese Patent Application No. 1-1376.
As shown in the specification of No. 63, it has the following physical and chemical properties.

(1) Molecular star: Asal+1pak GS-710 column using 50mM sodium chloride solution as the mobile phase (tJF cutoff molecular weight measured with pullulan is 100
00,000) using high performance liquid chromatography (hereinafter referred to as 1 (
When molecular sieving is performed using the abbreviation PLC,
A single peak is observed near the exclusion limit. Therefore, the size of the molecule is 1 Dalton or more, indicating an extremely large molecule size.

(2) Infrared absorption spectrum = β shows absorption at FJ900cm-', which is specific to glycosidic bonds.

(3) Basic, acidic, 2 neutral, p of an aqueous solution of two substances
11 is neutral.

(4) Viscosity A) Viscosity 2 The polysaccharide melt used in the present invention is a neutral J3 liquid with high tb property. When measuring the viscosity with a Visme 10-unit rotary viscometer, it is 1200-1700 for a 1% water solution.
Centipoise (adapter No. 3, 60 rotations, 30 seconds).

Stability against heat: It exhibits stable high viscosity under normal conditions and constant viscosity between 5°C and 80°C. It is also stable under autoclave heating (20 minutes) at 121°C and 1 atmosphere.

C) Stability against acids and alkalis: p 112-1
It shows a relatively stable viscosity in the range of 3.

2) Stability against salts It exhibits a certain viscosity and is stable in the presence of any salt such as diborate, acetate, sulfate, sodium salt, potassium salt, calcium salt, magnesium salt, etc.

(5) Safety test Multiple #M neck sensitization test (Mdxrt) used in the present invention
a: zaNon method 5 sample normal sample concentration % (twice).

When carrying out challenge (0.5% and 1%), 110 guinea pigs
None of the animals were found to be positive.

In addition, photosensitivity 2J of the polysaccharide used in the present invention:
(Adjuvant-5trip method, sample concentration: induction 1%.

When carrying out (0.5% and 1%), 10 guinea pigs
None of the animals were found to be positive.

From the following, the multi-tJ! used in the present invention! These polysaccharides have low sensitizing properties and are highly safe.

(6) Other characteristic properties Multi-I! used in the present invention! The species are tasteless and odorless.

Furthermore, the polysaccharide used in the present invention exhibits a silky feel when applied.

The β-glucan used in the present invention is disclosed in Japanese Patent Application No. 1-137
6G Obtained by the culture method and purification method shown in Specification B of No. 3. Further, the blending amount of β-glucan is preferably 0.05 to 5. Ogl r; 1% (hereinafter referred to as wt%
), more preferably 0.1~], Q w
It is L%. If it is less than 0.01 wt%, the thickening effect will be insufficient, which is not preferable. Moreover, if it is more than 5 WL%, the viscosity tends to become high and uniform dissolution of β-glucan becomes difficult, which is not preferable.

The cosmetics of the present invention may optionally include a moisturizing agent, a cosmetic medicinal ingredient, a fragrance, a preservative, a coloring agent, an ultraviolet absorber, an astringent, a synthetic surfactant, and an additive (kaolin, mica). , sericite, talc, yellow iron oxide, red iron oxide, etc.), water-soluble natural polymers (casein soda pectin, xantungum,
Karaya gum, locust bean gum, carrageenan, etc.)
can be added.

Examples of the cosmetics of the present invention include skin conditioning lotion, wiping lotion 5, softening lotion, acne treatment lotion, and aftershave lotion.

Double-layered conditioning lotion, cleansing lotion, hair toninx, masashi cream, cleansing cream, skin cream.

Examples include, but are not limited to, skin milk, foundation cream, makeup hair, hair cream, and the like.

[Examples] The present invention will be explained below with reference to Examples, but the cosmetics of the present invention are not limited to the description of Examples.

In addition, in order to evaluate the skin irritation during use and the sensory characteristics after use, a human skin punch test and a sensory test were conducted. The method is shown below.

(Human skin patch test) 0.05 g of the sample was applied to the skin of the pressure side of the forearm of 25 subjects for 24 hours using a patch test adhesive with a circular lint cloth attached for 24 hours. The skin condition of 25 subjects was evaluated for each sample according to the following criteria. The evaluation results are based on the stronger reaction between 1 hour and 24 hours after removal of the bandage, and the evaluation is (±
) or more people.

”υL1 unit (Sensory test) Test l- was conducted by 20 male and female subjects.

The evaluation was based on the number of people who answered that the product did not feel sticky after use.

Moreover, β-glucan used in this example was obtained by the following method.

(Example of production of β-glucan) 50! Put the following medium 3ON in a jar fermentor, inoculate it with the macrophomopsis strain KAB55 (@1st laboratory contract number 9366) pre-cultured in the following composition medium for 30 minutes, and incubate at 25°C with ventilation 51°C. ], Ov v
4E in m? It was cultured for a period of time.

(Composition) Glucose 3000gpha
rmamedia 150gKHtP0.
30g tap water
30 ffi (NaOI (prepared to p f (6)) The culture solution was centrifuged at 10,000 rpm to remove bacterial cells, and an equal volume of 60% ethanol was added to the resulting supernatant to remove polysaccharides. was precipitated.This was rotated too much/
The polysaccharide was obtained by centrifugation for 5 minutes and 5 minutes. The obtained polysaccharide was dissolved in water again and the above operation was repeated to obtain 25 g of tasteless, odorless, white and highly viscous β-glucan.

Examples 1-2. Comparative Examples 1 to 4 Lotion (composition) (8) Ethanol Hergamot oil 5.0 0, O5 Glycerin 5.0 Purified water Residual oil (b) 1st with total amount as loo
Thickener listed in Table After mixing and dissolving component (8) listed in Table 1, component ([1) was added and mixed and dispersed to prepare each lotion.

Examples 3-4. Comparative Examples 5 to 10 7L liquid ('ul composition) Raw material ingredients Mixture! il (wL%) (A) Cetyl alcohol 2.0 Octyldodecyl myristate 1.5 Liquid paraffin
2゜(B) Sodium cetyl sulfate 1.0 Methyl oxybenzoate 0.2 Propylene glycol 3.0 Purified water Total amount to 100
Remaining amount (C) Thickener listed in Table 1 Components (1) and (11) listed in Table 1 are heated and dissolved at 8o'C and mixed, and then component (C) is added. In addition, it was dispersed using a homomixer. Then att 30
Each emulsion was prepared by cooling at "c:i:".

Examples 5-7. Comparative Examples 11-16 Cream (possible) (A)'Nocerin 10.0 Liquid par, rough-in 25. O Cetyl Alcohol 1O101 Glycerin Nostearate 2.0 (B) Propylene Glycol
5.0 Sodium methyl sulfate 1
,0 methyl oxybenzoate 0.2 purified water
Remaining amount (A) component with total amount as 100 and (
B) Mix the components heated and dissolved at 80°C,
Furthermore, component (C) was added and dispersed using a homomixer.
Then, each cream was prepared by cooling to 30°C while stirring.

Example 1 containing β-glucan as shown in Table 1
The lotions, milky lotions, and creams of samples 1 to 7 had sensory characteristics (2) and were highly safe compared to Comparative Examples 1 to 1G.

[Effects of the Invention] As described above, the cosmetic of the present invention has no skin irritation and
U! The present invention provides cosmetics with excellent organoleptic properties, especially in terms of low stickiness.

Procedural amendment (voluntary) ■, Indication of the case 1999 Patent Application No. 305696 2, Name of the invention Cosmetics 3, Person making the amendment Relationship to the case Patent applicant address 17-4 Sumida 5-chome, Sumida-ku, Tokyo No. 1-5-90 Tomobuchi-cho, Bejima-ku, Osaka 534 Kanebo Co., Ltd. Patent Department Telephone (06) 921-1251 6. "Claims" and "Detailed Description of the Invention" of the specification subject to amendment
Column 7, Contents of amendment (1) Description: ``The description of the scope of claims is corrected as shown in the attached sheet.

(2) From line 19 on page 2 of the specification to line 9 on page 3, it is a cosmetic product characterized by containing β-glucan having a structure in which the binding mode is described below.

(a) The main 11 D-glucopyranose residues are all β-1,3 bonds, and Φ) The C-2 and/or of the D-glucopyranose residues in the main chain
or branched at the C-6 position, and the D-glucopyranose residue in the (C) side chain has β-
This cosmetic is characterized by containing highly viscous β-glucan having the following properties: 1,4 bonds are not present.

(a) The bonding mode is that all D-glucopyranose residues in the main chain are β-1,3 bonds, and the D-glucopyranose residue in the main chain is branched at the C-6 position, And there is no β-1,4 bond in the D-glucopyranose residue in its side chain.

(b) has a molecular weight of about 10 million Daltons or more as determined by high performance liquid chromatography using a molecular sieve column;

(C) The temperature dependence of viscosity has a constant viscosity between 5°C and 85°C at a concentration of 0.3% by weight or less,
It has a constant viscosity between 20°C and 85°C even at concentrations exceeding 0.3% by weight.

(d) The viscosity remains stable even after autoclave heating (20 minutes) at 121'c and 1kg/cm''.j
I am corrected.

8゜(3) From page 3, line 10 to line 11 of the specification, J
Delete certain.

(4) It exhibits a constant viscosity at r5°C to 80°C from line 13 to line 15 on page 4 of the specification.

In addition, autoclave heating at 121”C and 1 atm (
20 minutes). ” is replaced with “0.3% by weight
At the following concentrations, it has a constant viscosity between 5℃ and 85℃, and even at concentrations exceeding 0.3% by weight, it has a constant viscosity between 20℃ and 85℃.
It has a constant viscosity between Also l 21 ”C lower, 1k
The viscosity remains stable even after autoclave heating (20 minutes) at a concentration of After processing, it was 210 cps. Corrected to 1.

List of attached documents (1) Attachment (document containing the entire text of the amended scope of claims) 1 copy [Attachment] Claims Macrophomops is
) is produced by culturing microorganisms belonging to the genus (9). It is branched at the C-6 position of the residue, and there is no β-1,4 bond in the D-glucopyranose residue in its side chain.

0.3 times %of Eru But 20 ℃～85℃ And- of Ru Gen 1] Soto 4-

Claims (1)

[Claims] Macrophomopsis
A cosmetic comprising β-glucan produced by culturing microorganisms belonging to the genus and having a structure in the binding mode described below. (a) All D-glucopyranose residues in the main chain are β-1
, 3 bonds, and (b) C-2 and/or of the D-glucopyranose residue in the main chain.
or branched at the C-6 position, and the (c) side chain D-glucopyranose residue has β-1,
There are no 4 bonds.