JPH0585862B2 - - Google Patents
Info
- Publication number
- JPH0585862B2 JPH0585862B2 JP61024404A JP2440486A JPH0585862B2 JP H0585862 B2 JPH0585862 B2 JP H0585862B2 JP 61024404 A JP61024404 A JP 61024404A JP 2440486 A JP2440486 A JP 2440486A JP H0585862 B2 JPH0585862 B2 JP H0585862B2
- Authority
- JP
- Japan
- Prior art keywords
- adhesive
- sensor chip
- glass epoxy
- biosensor
- epoxy substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000853 adhesive Substances 0.000 claims description 21
- 230000001070 adhesive effect Effects 0.000 claims description 21
- 239000004593 Epoxy Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 230000005669 field effect Effects 0.000 claims description 7
- 239000012528 membrane Substances 0.000 description 8
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000010618 wire wrap Methods 0.000 description 1
Landscapes
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Photometry And Measurement Of Optical Pulse Characteristics (AREA)
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は血液や体液中に含まれる尿素を検出す
るためのバイオセンサに関するものである。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a biosensor for detecting urea contained in blood or body fluids.
（従来の技術）
従来、イオン感応性電界効果型トランジスタの
表面に酵素の固定化膜を設けたセンサチツプは、
チツプの状態では使用上困難であるため、ガラス
エポキシ基板上にセンサチツプを接着して用いて
いた。センサのトランジスタのリード線とガラス
エポキシ基板のリード線とがワイヤボンデイング
で接合され、このガラスエポキシ基板がコネクタ
を介して検出器に接続されていた。このセンサチ
ツプは、酵素の固定化膜を設けているため熱に弱
く、チツプをガラスエポキシ基板上に接着する接
着剤は、自然硬化型のものを使用していた。(Prior art) Conventionally, a sensor chip in which an enzyme immobilization film is provided on the surface of an ion-sensitive field effect transistor is
Since it is difficult to use the sensor chip in chip form, the sensor chip was used by bonding it to a glass epoxy substrate. The lead wires of the transistor of the sensor and the lead wires of the glass epoxy board were bonded together by wire bonding, and this glass epoxy board was connected to the detector via a connector. Because this sensor chip has an enzyme immobilization film, it is sensitive to heat, and the adhesive used to bond the chip to the glass epoxy substrate is a naturally curing type.
（発明が解決しようとする問題点）
上述した従来のバイオセンサは、自然硬化型の
接着剤を使用しているため、硬化するのに時間が
かかり作業性が悪く、また硬化するまで大気中に
長時間放置するため、センサチツプ上の酵素固定
化膜が一部失活するなどの欠点があつた。(Problems to be Solved by the Invention) The conventional biosensor described above uses a naturally curing adhesive, so it takes time to harden and has poor workability. There were drawbacks such as the enzyme-immobilized film on the sensor chip partially deactivated because it was left to stand for a long time.
本発明の目的は、このような欠点を除き、作業
性が良いと共に酵素固定化膜の失活をなくしたバ
イオセンサを提供することにある。 An object of the present invention is to eliminate such drawbacks, provide a biosensor that has good workability and eliminates deactivation of the enzyme-immobilized membrane.
（問題点を解決するための手段）
本発明の構成は、イオン感応性電界効果型トラ
ンジスタの表面に酵素の固定化膜を設けたセンサ
チツプを接着剤にてガラスエポキシ基板上に接着
していたバイオセンサにおいて、前記接着剤が紫
外性硬化型であると共に嫌気性の硬化特性をもつ
たものであることを特徴とする。(Means for Solving the Problems) The configuration of the present invention consists of a sensor chip having an enzyme immobilized film provided on the surface of an ion-sensitive field effect transistor, which is attached to a glass epoxy substrate with an adhesive. The sensor is characterized in that the adhesive is of an ultraviolet curing type and has anaerobic curing characteristics.
（作用）
本発明のバイオセンサによれば、センサチツプ
を紫外線硬化型であると共に嫌気性の硬化特性を
併せ持つた接着剤にてガラスエポキシ基板上に接
着するため、接着剤の硬化時間も極めて速く、自
然硬化型の接着剤と比べ接着途中で原液が硬化す
ることがなく作業性も良い。また、バイオセンサ
の製造時間を極めて単縮できるためセンサチツプ
上の酵素膜を失活させることがない。(Function) According to the biosensor of the present invention, since the sensor chip is bonded to the glass epoxy substrate using an adhesive that is UV-curable and has anaerobic curing properties, the curing time of the adhesive is extremely fast. Compared to naturally curing adhesives, the stock solution does not harden during bonding, making it easier to work with. Furthermore, since the manufacturing time of the biosensor can be extremely shortened, the enzyme membrane on the sensor chip is not deactivated.
（実施例） 次に本発明について図面を参照して説明する。(Example) Next, the present invention will be explained with reference to the drawings.
第１図は本発明の一実施例のバイオセンサの斜
視図、第２図は第１図のセンサチツプの接着方法
を示す側面図である。本実施例において、ガラス
エポキシ基板１の先端上面部にセンサチツプ２が
紫外線硬化型と嫌気性硬化型の混合接着剤３によ
り接着されている。この種の接着剤３としては、
東亜合成化学(株)製のアロンタイトST−1200や、
日本ロツクタイト(株)製のLI−298接着剤などがあ
る。センサチツプ２の上面には、イオン感応性電
界効果型トランジスタ４がパターン化されてお
り、このイオン感応性電界効果型トランジスタ４
の一部に酵素の固定化膜５が設けられている。こ
のイオン感応性電界効果型トランジスタ４のリー
ド線端部６と、ガラスエポキシ基板１の上面に設
けられた検出器用のリード線７の端部とはワイヤ
ボンデイング８で接続されている。 FIG. 1 is a perspective view of a biosensor according to an embodiment of the present invention, and FIG. 2 is a side view showing a method of bonding the sensor chip of FIG. 1. In this embodiment, a sensor chip 2 is bonded to the top surface of a glass epoxy substrate 1 using a mixed adhesive 3 of ultraviolet curing type and anaerobic curing type. As this type of adhesive 3,
Arontite ST-1200 manufactured by Toagosei Kagaku Co., Ltd.,
Examples include LI-298 adhesive manufactured by Nihon Loctite Co., Ltd. An ion-sensitive field effect transistor 4 is patterned on the top surface of the sensor chip 2.
An enzyme immobilization membrane 5 is provided in a part of the membrane. The lead wire end 6 of this ion-sensitive field effect transistor 4 and the end of a detector lead wire 7 provided on the upper surface of the glass epoxy substrate 1 are connected by wire bonding 8.
次に本実施例の接着方法について説明する。 Next, the adhesion method of this example will be explained.
第２図において、ガラスエポキシ基板１の上面
端部に嫌気性硬化特性の紫外線硬化型接着剤３を
薄く塗布し、その上面にセンサチツプ２を乗せ
て、センサチツプ２の端面より接着剤３が微量は
み出す程度押し付けてセンサチツプ２を安定させ
る。次にイオン感応性電界効果型トランジスタ４
の一部に設けられた酵素固定化膜５に紫外線が照
射しないようにマスク９を取り付ける。このマス
ク９を取り付けた後、紫外線ランプ１０を点灯し
て紫外線を照射する。紫外線ランプ１０の紫外線
照射により、センサチツプ２からはみ出した接着
剤３は約15秒で硬化する。このはみ出した接着剤
３が硬化すれば、センサチツプ２下面の接着剤３
は大気と遮断されるため嫌気性硬化特性により硬
化して接着が完了する。なおセンサチツプ２から
はみ出した接着剤３が硬化すれば、センサチツプ
２はガラスエポキシ基板１から、外れることがな
いため、次のワイヤラツピング工程に進むことが
できる。 In FIG. 2, an ultraviolet curing adhesive 3 with anaerobic curing properties is applied thinly to the upper end of the glass epoxy substrate 1, and a sensor chip 2 is placed on the upper surface, so that a small amount of the adhesive 3 protrudes from the end surface of the sensor chip 2. Press firmly to stabilize the sensor chip 2. Next, the ion-sensitive field effect transistor 4
A mask 9 is attached to prevent ultraviolet rays from irradiating the enzyme immobilization membrane 5 provided on a part of the membrane. After attaching this mask 9, the ultraviolet lamp 10 is turned on to irradiate ultraviolet rays. The adhesive 3 protruding from the sensor chip 2 is cured in about 15 seconds by the ultraviolet irradiation from the ultraviolet lamp 10. When this protruding adhesive 3 hardens, the adhesive 3 on the bottom surface of the sensor chip 2
Since it is isolated from the atmosphere, it hardens due to its anaerobic curing properties and completes the adhesion. Note that once the adhesive 3 protruding from the sensor chip 2 is cured, the sensor chip 2 will not come off from the glass epoxy substrate 1, and the process can proceed to the next wire wrapping step.
（発明の効果）
以上説明したように、本発明は、センサチツプ
の接着に嫌気性硬化特性を持つた紫外線硬化型接
着剤を使用したことにより接着剤の硬化時間を極
めて速くすることができ、バイオセンサの製造時
間を極めて単縮できるため、酵素膜を失活させる
ことがなくなるなどの効果がある。(Effects of the Invention) As explained above, the present invention uses an ultraviolet curable adhesive with anaerobic curing properties to bond sensor chips, thereby making it possible to extremely shorten the curing time of the adhesive and Since the manufacturing time of the sensor can be extremely shortened, there are effects such as eliminating the possibility of deactivating the enzyme membrane.
第１図は本発明の一実施例の斜視図、第２図は
第１図のセンサチツプの接着方法を示す断面図で
ある。
１……ガラスエポキシ基板、２……センサチツ
プ、３……接着剤、４……イオン感応性電界効果
型トランジスタ、５……酵素固定化膜、６……リ
ード線、７……検出器リード線、８……ワイヤボ
ンデイング、９……マスク、１０……紫外線ラン
プ。
FIG. 1 is a perspective view of one embodiment of the present invention, and FIG. 2 is a sectional view showing a method of bonding the sensor chip of FIG. 1. DESCRIPTION OF SYMBOLS 1...Glass epoxy substrate, 2...Sensor chip, 3...Adhesive, 4...Ion-sensitive field effect transistor, 5...Enzyme immobilization membrane, 6...Lead wire, 7...Detector lead wire , 8... wire bonding, 9... mask, 10... ultraviolet lamp.
Claims (1)
に酵素の固定化膜を設けたセンサチツプを接着剤
にてガラスエポキシ基板上に接着しているバイオ
センサにおいて、前記接着剤が紫外性硬化型であ
ると共に嫌気性の硬化特性をもつたものであるこ
とを特徴とするバイオセンサ。1. In a biosensor in which a sensor chip having an enzyme immobilized film provided on the surface of an ion-sensitive field effect transistor is adhered to a glass epoxy substrate with an adhesive, the adhesive is of an ultraviolet curing type and is anaerobic. A biosensor characterized by having a hardening property.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61024404A JPS62180261A (en) | 1986-02-05 | 1986-02-05 | Biosensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61024404A JPS62180261A (en) | 1986-02-05 | 1986-02-05 | Biosensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62180261A JPS62180261A (en) | 1987-08-07 |
| JPH0585862B2 true JPH0585862B2 (en) | 1993-12-09 |
Family
ID=12137228
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61024404A Granted JPS62180261A (en) | 1986-02-05 | 1986-02-05 | Biosensor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62180261A (en) |
-
1986
- 1986-02-05 JP JP61024404A patent/JPS62180261A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62180261A (en) | 1987-08-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |