JPH0570503A - Method for stabilizing polysaccharide - Google Patents
Method for stabilizing polysaccharideInfo
- Publication number
- JPH0570503A JPH0570503A JP3234712A JP23471291A JPH0570503A JP H0570503 A JPH0570503 A JP H0570503A JP 3234712 A JP3234712 A JP 3234712A JP 23471291 A JP23471291 A JP 23471291A JP H0570503 A JPH0570503 A JP H0570503A
- Authority
- JP
- Japan
- Prior art keywords
- acidic polysaccharide
- aqueous solution
- viscosity
- plant belonging
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明はポリアンテス属に属する
植物のカルスが生産する酸性多糖類を含有する水性溶液
の安定化方法に関する。TECHNICAL FIELD The present invention relates to a method for stabilizing an aqueous solution containing an acidic polysaccharide produced by callus of a plant belonging to the genus Polyantes.
【０００２】[0002]
【従来の技術】一般に、その使用感触の良好さから、適
度の粘度を有する水溶液、乳液、ジェル等の形態の外用
剤や化粧料等が提供されており、従来、これらの増粘剤
又はゲル化剤としては種々の高分子物質が用いられてい
る。2. Description of the Related Art In general, external preparations and cosmetics in the form of aqueous solutions, emulsions, gels and the like having appropriate viscosities have been provided because of their good feeling in use. Conventionally, these thickeners or gels have been provided. Various polymer substances are used as the agent.
【０００３】また、動物や植物由来の多糖類は優れた増
粘作用やゲル化作用を有し、増粘剤、ゲル化剤、気泡安
定剤、懸濁・乳化安定剤、被膜形成剤、粘着剤及び生理
活性剤等として広く利用されている。Further, animal- or plant-derived polysaccharides have an excellent thickening action and gelling action, and they are thickeners, gelling agents, foam stabilizers, suspension / emulsion stabilizers, film-forming agents, adhesive agents. Widely used as agents and bioactive agents.
【０００４】しかしながら、一般に多糖類を含有する水
性溶液は長期にわたって粘度を維持するのは難しいとい
う問題があった。そこで、例えば動物由来の多糖類であ
るヒアルロン酸の場合は、ポリアクリル酸、ポリアミノ
酸のような高分子を添加したり、フェノール性水酸基を
有する化合物を添加することにより、粘度の安定化をは
かることが行われていた（特開昭５７−１８５２０８号
公報、特開平１−１１３４０１号公報）。しかし、この
方法によっても未だ満足し得る結果は得られていなかっ
た。However, there is a problem that it is generally difficult to maintain the viscosity of an aqueous solution containing a polysaccharide for a long period of time. Therefore, for example, in the case of hyaluronic acid, which is a polysaccharide derived from animals, the viscosity is stabilized by adding a polymer such as polyacrylic acid or polyamino acid or a compound having a phenolic hydroxyl group. (Japanese Patent Application Laid-Open No. 57-185208, Japanese Patent Application Laid-Open No. 1-113401). However, satisfactory results have not yet been obtained by this method.
【０００５】一方、ポリアンテス属に属する植物のカル
スが生産する酸性多糖類が、洗浄剤組成物、外用剤組成
物中に配合することにより、皮膚の保湿効果、保護効果
を高めるとともに、皮膚を柔軟、平滑化し、しかも洗浄
剤組成物、外用剤組成物本来の機能に何ら影響を与える
ことなく使用時及び使用後の感触を改善することが知ら
れている（特開平１−２１３２１３号公報、特開平１−
２１３２２５号公報）。On the other hand, when an acidic polysaccharide produced by callus of a plant belonging to the genus Polyantes is mixed in a cleaning composition or an external preparation composition, the moisturizing effect and the protective effect on the skin are enhanced and the skin is softened. It is known to improve the feel during use and after use without any effect on the original functions of the cleaning composition and the external preparation composition (Japanese Patent Application Laid-Open No. 1-213213, JP Kaihei 1-
No. 213225).
【０００６】しかしながら、この酸性多糖類も水性溶液
にした場合、低温〜室温保存においては何ら特性の変化
は見られないが、高温保存においては不安定であり、１
〜２週間で経時的に粘度が低下し、またそれに伴って感
触も損われるという問題を有していた。現在のところ、
この経時的な粘度低下の原因は必ずしも明確にされては
おらず、またその抑制法についてもまったく知られてい
ない。However, when this acidic polysaccharide is also made into an aqueous solution, no change in properties is observed at low temperature to room temperature storage, but it is unstable at high temperature storage.
There has been a problem that the viscosity decreases with time in about 2 weeks, and the feel is impaired accordingly. at present,
The cause of this decrease in viscosity with time has not always been clarified, and no method for suppressing it has been known at all.
【０００７】[0007]
【発明が解決しようとする課題】従って、ポリアンテス
属に属する植物のカルス培養物由来の酸性多糖類を含有
する水性溶液の高温における粘度低下を抑制し、長期安
定保存を可能とする方法の開発が望まれていた。Therefore, the development of a method for suppressing long-term stable storage by suppressing a decrease in viscosity of an aqueous solution containing an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyantes, at a high temperature, has been developed. Was wanted.
【０００８】[0008]
【課題を解決するための手段】斯かる実情において、本
発明者らは、上記課題を解決すべく鋭意検討を行った結
果、ポリアンテス属に属する植物のカルス培養物由来の
酸性多糖類を含有する水性溶液に、特定の天然酸性多糖
類を添加すれば、経時的粘度の低下が抑えられると共に
長期保存安定性を向上させることができることを見出
し、本発明を完成した。Under such circumstances, the present inventors have conducted diligent studies to solve the above-mentioned problems, and as a result, contain an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes. The present invention has been completed based on the finding that addition of a specific natural acidic polysaccharide to an aqueous solution can suppress the decrease in viscosity with time and improve long-term storage stability.
【０００９】すなわち、本発明は構成糖としてキサンタ
ンガム、ゲランガム、アルギン酸、ペクチン及びヒアル
ロン酸より選ばれる天然酸性多糖類を添加することを特
徴とする、ポリアンテス属に属する植物のカルス培養物
由来の酸性多糖類を含有する水性溶液の安定化方法を提
供するものである。That is, the present invention is characterized in that a natural acidic polysaccharide selected from xanthan gum, gellan gum, alginic acid, pectin and hyaluronic acid is added as a constituent sugar, and an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes is added. A method for stabilizing an aqueous solution containing a saccharide is provided.
【００１０】本発明における、ポリアンテス属に属する
植物のカルス培養物由来の酸性多糖類（以下、「酸性多
糖類」と称する）は、例えば、特開昭６４−１０９９７
号公報記載の方法に従って、ポリアンテス属に属する植
物から誘導されるカルスを培養し、得られた培養物から
採取することができる。In the present invention, an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyantes (hereinafter referred to as "acidic polysaccharide") is disclosed in, for example, JP-A-64-10997.
Callus derived from a plant belonging to the genus Polyantes can be cultured according to the method described in the publication, and the obtained culture can be collected.
【００１１】ここで、酸性多糖類の採取方法をポリアン
テス属に属する植物として、チューベロース（Ｐｏｌｉ
ａｎｔｈｅｓ ｔｕｂｅｒｏｓａ Ｌ．）を例に挙げて
示せば、チューベローズの花等の一部を外植片として、
Ｌｉｎｓｍａｉｅｒ−Ｓｋｏｏｇの基本培地に植物ホル
モンとして１０-5Ｍのオーキシン及び１０-6Ｍのサイト
カイニンを添加し、更に炭素源として３％のサッカロー
スを添加した培地を用いてカルスを誘導した後、継代培
養を行い、更に上記カルス培養培地と同様の成分からな
る液体培地を用いて振とう培養する。その後、培養液か
ら遠心分離またはろ過等によって細胞を除去し、培養液
をロータリーエバポレーター等を用いて濃縮し、濃縮液
にエタノール、アセトン等の溶媒を加えて沈澱させ、沈
澱物を凍結乾燥することにより酸性多糖類を分離、取得
することができる。[0011] In this case, as a plant belonging to the sampling method of acidic polysaccharides in Poriantesu genus, Chuberosu (Poli
anthes tuberosa L. ) As an example, a part of a tuberose flower or the like is used as an explant,
Linusmaier-Skoog basal medium was supplemented with 10 -5 M auxin and 10 -6 M cytokinin as plant hormones, and callus was induced using a medium supplemented with 3% saccharose as a carbon source. Culturing is carried out, and shaking culture is carried out using a liquid medium containing the same components as the above callus culture medium. After that, remove cells from the culture solution by centrifugation or filtration, concentrate the culture solution using a rotary evaporator, etc., add a solvent such as ethanol or acetone to the concentrated solution for precipitation, and freeze-dry the precipitate. Thus, the acidic polysaccharide can be separated and obtained.
【００１２】斯くして得られた酸性多糖類の水性溶液中
の濃度は特に制限されるものではなく、水性媒体中に溶
解可能な濃度まで使用し得る。The concentration of the acidic polysaccharide thus obtained in the aqueous solution is not particularly limited, and it can be used up to a concentration at which it can be dissolved in an aqueous medium.
【００１３】本発明において、酸性多糖類を含有する水
性溶液の安定化のために添加される、天然酸性多糖類は
キサンタンガム、ゲランガム、アルギン酸、ペクチン及
びヒアルロン酸より選ばれるものであるが、特に好まし
いのはキサンタンガム、ゲランガム、ペクチンである。
これらは一種又は二種以上を併用して用いることがで
き、水性溶液中に０．０００１〜１０重量％、特に０．
００１〜５重量％添加されることが好ましい。斯くする
ことにより当該水性溶液の経時的粘度低下の防止性及び
安定性を向上させることができる。In the present invention, the natural acidic polysaccharide added for stabilizing the aqueous solution containing the acidic polysaccharide is selected from xanthan gum, gellan gum, alginic acid, pectin and hyaluronic acid, but is particularly preferable. Are xanthan gum, gellan gum and pectin.
These can be used alone or in combination of two or more, and 0.0001 to 10% by weight, particularly 0.
It is preferable to add 001 to 5% by weight. By doing so, it is possible to improve the prevention property and stability of the viscosity decrease of the aqueous solution with time.
【００１４】尚、本発明において酸性多糖類を含有する
水性溶液中には、上記の天然酸性多糖類以外の成分、例
えば塩化ナトリウム、塩化カルシウム、塩化マグネシウ
ム、リン酸水素ナトリウム等の無機塩類、乳酸ナトリウ
ム等の有機酸塩、水酸化カリウム等の水酸化物、グリセ
リン、プロピレングリコール、１，３−ブチレングリコ
ール、ジプロピレングリコール、ポリエチレングリコー
ル、ポリオキシエチレンメチルグルコシド等の分子内に
２個以上の水酸基を持つ水溶性多価アルコール、グルコ
ース、シュークロース、ソルビトール、マルチトール等
の糖類、クエン酸、乳酸等の有機酸、エタノール、アミ
ノ酸、尿素、タンパク質、界面活性剤、パラオキシ安息
香酸エステル等の有機物が溶解していてもよく、また、
水性媒体に不溶なオリーブ油、ワセリン、流動パラフィ
ン、高級アルコール等が懸濁していてもよい。In the present invention, the aqueous solution containing an acidic polysaccharide contains components other than the above-mentioned natural acidic polysaccharides, for example, inorganic salts such as sodium chloride, calcium chloride, magnesium chloride, sodium hydrogenphosphate, and lactic acid. Two or more hydroxyl groups in the molecule such as organic acid salts such as sodium, hydroxides such as potassium hydroxide, glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, polyethylene glycol and polyoxyethylene methyl glucoside. With water-soluble polyhydric alcohols, sugars such as glucose, sucrose, sorbitol, maltitol, organic acids such as citric acid and lactic acid, and organic substances such as ethanol, amino acids, urea, proteins, surfactants, and paraoxybenzoic acid esters. It may be dissolved,
Olive oil, petrolatum, liquid paraffin, higher alcohols and the like which are insoluble in the aqueous medium may be suspended.
【００１５】[0015]
【発明の効果】本発明の安定化方法を用いれば、ポリア
ンテス属に属する植物のカルス培養物由来の酸性多糖類
を含有する水性溶液の高温における粘度低下を抑制し、
かつ安定性を長期にわたって向上させることができる。
従って、本発明の安定化方法を適用した水性溶液は、適
度な粘度を高温においても長期間維持できるため、これ
を用いれば、使用感触の良好な水溶液、乳液、ジェル等
の形態の外用剤、化粧料あるいは洗浄剤等が得られる。EFFECT OF THE INVENTION By using the stabilizing method of the present invention, it is possible to suppress a decrease in viscosity at high temperature of an aqueous solution containing an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes,
And stability can be improved over a long period of time.
Therefore, the aqueous solution to which the stabilizing method of the present invention is applied can maintain an appropriate viscosity for a long period of time even at a high temperature. Therefore, when using this, an aqueous solution having a good feeling in use, an emulsion, an external preparation in the form of a gel, etc. Cosmetics or detergents can be obtained.
【００１６】[0016]
【実施例】以下に実施例を挙げて本発明を更に説明する
が、本発明はこれによって何ら限定されるものではな
い。EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited thereto.
【００１７】参考例 酸性多糖類の製造： ポリアンテス属に属する植物として、チューベローズ
（Ｐｏｌｉａｎｔｈｅｓｔｕｂｅｒｏｓａ Ｌ．）を用
いた。カルスは減菌したチューベローズの開花２〜７日
前の蕾みを植物ホルモンとして１×１０-5Ｍのα−ナフ
タレン酢酸と１×１０-6Ｍのベンジルアデニンを含み、
炭素源として３％のサッカロースを含むＬｉｎｓｍａｉ
ｅｒ−Ｓｋｏｏｇの培地（寒天培地）を用いて誘導し
た。誘導されたカルスは同培地で継代培養した。数代以
上に継代し安定化したカルスを植物ホルモンとして１×
１０-5Ｍの２，４−ジクロロフェノキシ酢酸を含み、炭
素源として５％のサッカロースを含むＬｉｎｓｍａｉｅ
ｒ−Ｓｋｏｏｇの培地（液体培地）に５％濃度となるよ
う接種した。培養は暗所にてロータリーシェーカーを用
いて振とう数１２０r.p.m.、２７±１℃で３０日間行っ
た。この後、ろ過及び遠心分離により、培養液から細胞
を取り除き、これをロータリーエバポレーターを用いて
濃縮した。この濃縮液に約３倍量のエタノールを加え、
５℃で２４時間静置し沈澱を得た。この沈澱を遠心分離
によって回収し、７０％エタノールで３回洗浄した後、
凍結乾燥により水分を除去し、目的とする酸性多糖類を
得た。Reference Example Production of Acidic Polysaccharide: As a plant belonging to the genus Polyanthes, tuberose ( Polyanthestuberosa L. ) was used. Callus contains 1 × 10 −5 M α-naphthalene acetic acid and 1 × 10 −6 M benzyladenine as plant hormones of the buds of the sterilized tuberose 2 to 7 days before flowering.
Linsmai with 3% sucrose as carbon source
Induction was performed using er-Skoog's medium (agar medium). The induced callus was subcultured in the same medium. Callus that has been passaged for several generations or more and stabilized as a plant hormone
Linsmaie containing 10 −5 M 2,4-dichlorophenoxyacetic acid and 5% sucrose as carbon source.
The medium (liquid medium) of r-Skoog was inoculated to have a concentration of 5%. The culture was carried out in a dark place using a rotary shaker at a shaking number of 120 rpm at 27 ± 1 ° C. for 30 days. After this, the cells were removed from the culture solution by filtration and centrifugation, and this was concentrated using a rotary evaporator. About 3 times the amount of ethanol was added to this concentrated solution,
A precipitate was obtained by standing at 5 ° C. for 24 hours. The precipitate was recovered by centrifugation, washed 3 times with 70% ethanol,
Water was removed by freeze-drying to obtain the target acidic polysaccharide.
【００１８】実施例１ 参考例で得られた酸性多糖類の１重量％水溶液に下記組
成中のペクチン以外の成分を添加し、次いでペクチンを
添加し、最終的に下記組成の酸性多糖類含有水溶液を調
製した。これを５０℃にて保存し、ペクチン添加直後、
添加１ケ月後、添加２ケ月後の粘度をＢ型粘度計を用い
て測定し、その粘度保存率を求めた。添加直後の粘度を
１００％としたときの結果を表１に示す。尚、比較とし
て、ペクチンを添加しないものについて同様の粘度測定
操作を行った。 （配合組成） 酸性多糖類 ０．３（重量％） メチルパラペン ０．１ ９５°変性アルコール ８ リン酸水素ナトリウム ０．７１ クエン酸 ０．２５ ペクチン ０．３ ８６°グリセンリン １０ イオン交換水 バランスExample 1 To a 1% by weight aqueous solution of the acidic polysaccharide obtained in Reference Example, components other than pectin in the following composition were added, then pectin was added, and finally an acidic polysaccharide-containing aqueous solution having the following composition was added. Was prepared. Store this at 50 ℃, immediately after adding pectin,
The viscosity one month after the addition and two months after the addition were measured using a B-type viscometer to determine the viscosity storage rate. Table 1 shows the results when the viscosity immediately after the addition was 100%. In addition, as a comparison, the same viscosity measurement operation was carried out for those without pectin added. (Composition) Acidic polysaccharide 0.3 (wt%) Methyl parapen 0.1 95 ° denatured alcohol 8 Sodium hydrogen phosphate 0.71 Citric acid 0.25 Pectin 0.3 86 ° Glyceline 10 Ion exchange water balance
【００１９】[0019]
【表１】 [Table 1]
【００２０】表１の結果から、天然酸性多糖類を共存さ
せることで、酸性多糖類含有水溶液の粘度低下を抑制で
きることがわかる。From the results shown in Table 1, it can be seen that the coexistence of natural acidic polysaccharides can suppress the decrease in viscosity of the acidic polysaccharide-containing aqueous solution.
【００２１】実施例２ 参考例で得られた酸性多糖類の１重量％水溶液に下記組
成中の添加物以外の成分を添加し、次いで添加物を添加
し、最終的に下記組成の酸性多糖類含有水溶液を調製し
た。これらについて実施例１と同様にして添加物添加直
後、添加１週間後、添加２週間後、添加４週間後の粘度
保存率を求めた。結果を表２に示す。尚、比較として、
グリセリン、ポリエチレングリコール６０００を添加し
たもの、添加物なしのものについて同様の粘度測定操作
を行った。 （配合組成） 酸性多糖類 ０．２（重量％） メチルパラペン ０．１ ９５°変性アルコール ８ 添加物 表２に示す量 イオン交換水 バランスExample 2 To the 1% by weight aqueous solution of the acidic polysaccharide obtained in Reference Example, components other than the additive in the following composition were added, and then the additive was added, and finally the acidic polysaccharide having the following composition. A containing aqueous solution was prepared. For these, in the same manner as in Example 1, the viscosity preservation ratio was determined immediately after the addition of the additive, 1 week after the addition, 2 weeks after the addition, and 4 weeks after the addition. The results are shown in Table 2. As a comparison,
The same viscosity measurement operation was performed for those with glycerin and polyethylene glycol 6000 added and those without additives. (Compounding composition) Acidic polysaccharide 0.2 (wt%) Methyl parapen 0.1 95 ° denatured alcohol 8 Additive Amount shown in Table 2 Ion-exchanged water balance
【００２２】[0022]
【表２】 [Table 2]
【００２３】表２の結果から、天然酸性多糖類を共存さ
せることで、酸性多糖類含有水溶液の粘度低下を抑制で
きることがわかる。From the results shown in Table 2, it can be seen that the coexistence of natural acidic polysaccharides can suppress the decrease in viscosity of the acidic polysaccharide-containing aqueous solution.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ａ６１Ｋ 47/36 Ｆ 7329−4Ｃ ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Internal reference number FI technical display area A61K 47/36 F 7329-4C
Claims (1)
酸、ペクチン及びヒアルロン酸より選ばれる天然酸性多
糖類を添加することを特徴とする、ポリアンテス属に属
する植物のカルス培養物由来の酸性多糖類を含有する水
性溶液の安定化方法。1. An aqueous solution containing an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes, characterized in that a natural acidic polysaccharide selected from xanthan gum, gellan gum, alginic acid, pectin and hyaluronic acid is added. Stabilization method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3234712A JP2995505B2 (en) | 1991-09-13 | 1991-09-13 | How to stabilize polysaccharides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3234712A JP2995505B2 (en) | 1991-09-13 | 1991-09-13 | How to stabilize polysaccharides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0570503A true JPH0570503A (en) | 1993-03-23 |
| JP2995505B2 JP2995505B2 (en) | 1999-12-27 |
Family
ID=16975201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3234712A Expired - Lifetime JP2995505B2 (en) | 1991-09-13 | 1991-09-13 | How to stabilize polysaccharides |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2995505B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6271001B1 (en) | 1995-03-23 | 2001-08-07 | Bio Polymers Pty. Ltd. | Cultured plant cell gums for food, pharmaceutical, cosmetic and industrial applications |
| US8900158B2 (en) | 2007-03-09 | 2014-12-02 | Fujitsu Limited | Exercise monitoring device, exercise monitoring program storage medium, and exercise monitoring method |
-
1991
- 1991-09-13 JP JP3234712A patent/JP2995505B2/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6271001B1 (en) | 1995-03-23 | 2001-08-07 | Bio Polymers Pty. Ltd. | Cultured plant cell gums for food, pharmaceutical, cosmetic and industrial applications |
| US6350594B1 (en) | 1995-03-23 | 2002-02-26 | Bio Polymers Pty. Ltd. | Cultured plant cell gums for food, pharmaceutical, cosmetic and industrial applications |
| US8900158B2 (en) | 2007-03-09 | 2014-12-02 | Fujitsu Limited | Exercise monitoring device, exercise monitoring program storage medium, and exercise monitoring method |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2995505B2 (en) | 1999-12-27 |
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