JPH0570339A - Composition for makeup - Google Patents

Abstract

PURPOSE:To obtain a safe composition for makeup, containing a solubilizing factor of animal hair, above all, wool, capable of supplying water or a humectant ingredient to a keratinous layer of the skin and regulating the skin conditions and having effects on maintenance of the skin always young. CONSTITUTION:A composition for makeup is obtained by providing a solubilizing factor of animal hair using a solubilizing treatment method according to an oxidizing decomposition method with an oxidizing agent (hydrogen peroxide is especially preferred) at a relatively high concentration in a weak alkaline liquid medium without inactivating physiological activity of the factor and including the resultant factor. Typical examples of the animal hair used are wool and further alpaca, mohair, angola, cashmere, etc. A solubilizing factor prepared by further chemically modifying the solubilizing factor of the animal hair is used or a solubilizing factor prepared by introducing hydrophilic groups is preferably used. The amount of the blended solubilizing factor for the animal hair is preferably about 0.1-5.0wt.% based on the whole weight of the composition for makeup.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to cosmetic compositions.

[0002]

2. Description of the Related Art The epidermis, which is the most surface of the skin, is a stratified squamous epithelium composed of five layers of stratum corneum, transparent layer, granule layer, spinous layer and basal layer and has a thickness of 0.1 to 0.3 mm. is there. While the epidermal cells generated in the basal layer are pushed up to the surface of the skin, a hard protein (keratin) is generated inside the cell, the keratin gradually fills the inside of the cell, and finally becomes dry and loses life. It becomes keratinocytes. It takes about 2 weeks during this period, and 2 weeks after that, the keratinocytes become dandruff or dirt and peel off from the epidermis. That is, the newly formed epidermal cells detach from the epidermis in about 4 weeks.

Healthy and beautiful skin means (1) moderately moisturized, fresh, soft and elastic, (2) moderately shiny and firm, (3) complexion and lively skin. Conceivable. In order to maintain the health of the skin, water (sweat) and oil (sebum) are constantly secreted from the skin glands, and efforts are made to keep the water content of the stratum corneum at 10 to 20%. One of the roles of cosmetics is to supplement the stratum corneum with an appropriate amount of water and oil,
To condition the skin.

As a cosmetic composition for maintaining healthy and beautiful skin, a composition containing the following components is known. Ingredients that help retain water in the stratum corneum of the skin are collectively called moisturizers, which are humectants.
ants) and emollients. As a moisturizing agent, a moisturizing agent that retains moisture in the stratum corneum to keep it moisturizing and flexible, for example, glycerin, propylene glycol (PG), polyethylene glycol (PEG), sorbitol, and NMF (natural moisturizing fact)
dl-pyrrolidone carboxylate, which is also known as a component of or), hyaluronate, chondroitin sulfate, collagen and the like, which have recently been highlighted as dermal components.

In the conventional cosmetic composition, the moisturizing chemical substances, the amino acids contained in the dermis and the acidic mucopolysaccharides are used alone or in combination as described above. Originally, it is considered physiologically desirable to impart moisturizing properties with a protein similar to that of epidermal cells. By the way, unlike the protein of the stratum corneum of the epidermis, collagen is an extremely dilute protein having a sulfur-containing component. On the other hand, there have been very few cases where keratin protein has been positively used as a cosmetic composition. Generally, keratin protein is not commercially available, and as a cosmetic composition,
It was extremely difficult to process it into usable physical properties.

[0006]

DISCLOSURE OF THE INVENTION The present invention provides a cosmetic composition containing a protein almost similar to that of the stratum corneum of the epidermis, and having an excellent action of replenishing the stratum corneum of the skin with water and moisturizing ingredients to condition the skin. The purpose is to provide goods.

[0007]

That is, the present invention relates to a cosmetic composition containing a solubilizing factor for animal hair.

Animal hair that can be used in the present invention is typically wool, and other examples include alpaca, mohair, angora, cashmere, etc., but are not limited thereto.

Animal hair is formed by differentiation of epidermal cells of animals, and its main component is insoluble keratin protein. Therefore, in order to use animal hair as a cosmetic agent, it is necessary to solubilize the wool first.

The animal hair solubilizing factor used in the present invention may be prepared by a solubilizing treatment method which does not inactivate the physiological activity of the factor. As such a solubilization treatment method, an oxidative decomposition method using a relatively high concentration of an oxidizing agent in a weak alkaline liquid medium (patent pending, Japanese Patent Application No. 2-248456)
No.), an enzymatic method using various proteolytic enzymes that hydrolyze keratin, which is the main component of animal hair, and a reduction method using mercaptoethanol or mercaptoethylamine, etc. The oxidative decomposition method is particularly preferable from the viewpoints that it can be regenerated as a solid, can be treated on an industrial scale, and the like, and will be described in more detail.

The liquid medium used in the oxidative decomposition method is generally water or alcohols (eg, methanol, ethanol and propanol), and if desired, two or more kinds may be used in combination.

P of these liquid media to the weak alkaline region
Examples of the H modifier include ammonia, alkali metal hydroxides, amines and alkali metal carbonates,
These may be appropriately selected according to the liquid medium used, the type of oxidant, and the like.

Examples of the oxidizing agent include peroxides such as hydrogen peroxide, peracetic acid, and formic acid, which are inexpensive, easy to handle, easy to post-treat after solubilizing animal hair, and solubilizing products. Hydrogen peroxide is most preferred because it does not leave harmful components inside.

The concentration of the oxidizing agent is usually 20% or more, preferably 25 to 35%.

The solubilization of animal hair depends on the type and concentration of the oxidizing agent used and the type of dissolution medium, but the solubilization time is generally about 0.1 to 1.0 hour. For example, when using treated water whose pH is adjusted to about 8 using 35% hydrogen peroxide and ammonia, when animal hair is immersed, the temperature rises naturally to about 100 ° C and the temperature can be increased within 1 hour. The solubilization is complete and almost no undissolved material remains.

The animal hair solubilizing factor prepared by the above-mentioned solubilization treatment is a peptide having a molecular weight of about 1000 to about 2,000,000 and having an amino acid composition substantially similar to that of the raw material animal hair.

Although cystine contained in animal hair may be decomposed or converted into a cystine derivative such as cystic acid by the solubilization treatment of animal hair, the physiological activity of the solubilizing factor is not affected by these products. I do not receive it.

The solubilizing factor consisting of the peptide having a molecular weight of about 1000 to 2,000,000 obtained by the above solubilization treatment [the main component is a peptide having a molecular weight of several thousand to several hundred thousand] may be used as it is. However, in order to remove impurities, processing agents, etc. that are mixed in during the manufacturing process, etc., it may be subjected to further separation and purification treatment and then used. As a method for separation and purification, means commonly used in the art, for example, fractionation with a solvent, salting out, pH treatment and various chromatographies may be appropriately used. In this case, specific components may be separated and used according to the purpose.

The solubilized animal hair can be regenerated as a protein in a convenient state according to the type of the intended cosmetic product. For example, in the case of a lotion used as a basic cosmetic, protein solubility is important. The regenerated protein (dried and powdered) obtained by adding an acid to the animal hair protein solution to cause gelation and mixing with ethanol is considerably soluble. Therefore, it is suitable as a lotion composition. On the other hand, if zinc or citric acid, which is a component of the astringent lotion, is used, the animal hair protein solubilized by oxidative decomposition can be recovered. Therefore, it can be used as a composition of astringent lotion and powder pack. In addition, in the present invention, a solubilizing factor obtained by chemically modifying at least a part of the above-mentioned animal hair solubilizing factor may be used. For example, the water solubility of the solubilizing factor may be improved by introducing a hydrophilic group by succinylation of the animal hair solubilizing factor with succinic anhydride or hydroxylation with epichlorohydrin or ethylene oxide.

The cosmetic composition of the present invention comprises, in addition to a solubilizing factor for animal hair, an alcohol usually used in cosmetic compositions,
It is obtained by preparing with purified water containing a moisturizer, a solubilizer, a fragrance, an antiseptic, and other components.

The content of the animal hair solubilizing factor is 0.1 to 5.0% by weight based on the total weight of the cosmetic composition.

Examples of alcohols include ethanol and isopropanol, and the blending amount thereof is preferably 0 to 30% by weight based on the total weight of the cosmetic composition.

Moisturizers include glycerin, sorbit, propylene glycol, dipropylene glycol,
1,3-butylene glycol, polyethylene glycol
(200, 400, 1000, 1500, 4000), polyhydric alcohols, saccharides, amino acids and the like, and the blending amount thereof is preferably 0 to 15% by weight based on the total weight of the cosmetic composition.

As the solubilizer, a surfactant having a high HLB, such as polyoxyethylene oleyl ether, polyoxyethylene sorbitan monolaurate,
Examples thereof include polyoxyethylene lauryl ether, and the amount thereof is preferably 0 to 3.0% by weight based on the total weight of the cosmetic composition.

The perfume and preservative may be blended in appropriate amounts.

Other ingredients include emollients
(Oleyl alcohol etc.), colorants, antioxidants, ultraviolet absorbers, astringents (zinc paraphenol sulfonate etc.) and the like.

[0027]

EXAMPLES The present invention will be described below with reference to examples, but the present invention is not limited to these examples. In addition,
In the examples, “%” indicates “% by weight”.

Production Example To 100 g of wool, 500 ml of 35% hydrogen peroxide solution was added, and the pH was adjusted to 8.3 with aqueous ammonia. This is left at room temperature for 1 hour, and after confirming that the wool has been solubilized, p
Depressed H. Alcohol was added to this solution and left overnight.
Then, the supernatant was discarded and the wool protein recovered as a precipitate was further washed with alcohol. Finally, it was washed with acetone and air dried. By this operation, about 70 g of a very pale yellow, tasteless and odorless powder (wool solubilizing factor) was obtained.

The solubility of the obtained solubilizing factor in various buffer solutions and alcohols was examined. Table 1
As shown in, the solubilizing factor obtained by this method is pH7.
-9, especially at pH 8 or higher, it dissolved well. Also, it was well dissolved in 50% or less of alcohol.

[0030]

[Table 1]

Next, the amino acid composition of this factor was analyzed.
When the raw material wool and the obtained solubilizing factor were compared, no significant difference was found between the two with respect to amino acids other than cystine (see Table 2). The solubilizing factor contained cystic acid produced by the oxidation of cystine during the process of oxidative degradation.

[0032]

[Table 2]

Example 1 and Comparative Example 1 A lotion composition was prepared with the following formulation using the wool protein powder obtained in the same manner as in Production Example. Protein powder 2.0% Polyoxyethylene sorbitan monolauric acid ester (20EO) 2.0% Oleyl alcohol 0.1% Ethanol 10.0% Perfume 0.1% Coloring agents, antioxidants (appropriate to purified water The total amount was set to 100 ml.) When the skin lotion was applied to the skin, the skin was softly retained and moistened. Lotion using polyethylene glycol 1000 instead of protein powder (Comparative Example 1)
When the lotion was prepared and compared with the above lotion, the effect using the polyethylene glycol 1000 was scarcely left, although the lotion according to the method of the present invention had the effect even after 6 hours.

Example 2 and Comparative Example 2 Wool was solubilized in the same manner as in Example 1. Then citric acid 2
Mixed with 0 g / 1000 ml aqueous solution. After standing for 2 hours, the supernatant was discarded and the wool protein recovered as a precipitate was further washed with the above citric acid aqueous solution. Then, it was dried under reduced pressure to obtain about 60 g of a pale yellow powder. Using this powder, a lotion composition was prepared according to the following formulation. Protein powder 3.0% Glycerin 1.0% Ethanol 15.0% Fragrance 0.2% Solubilizer 1.0% (Purified water was used to make 100 ml.) The above lotion was applied to the skin. The skin was tenderly tightened and astringency was observed. When a composition (Comparative Example 2) obtained by removing the protein powder from the formulation of Example 2 was prepared and compared with the lotion of Example 2, no astringency was observed.

Example 3 The following composition was prepared using the same protein powder as in Example 2. Protein powder 2.0% Zinc paraphenol sulfonate 0.1% Sorbit 2.0% Glycerin 2.0% Polyoxyethylene oleyl ether (20EO) 1.0% Perfume 0.1% Ethanol 15.0% Preservative: Appropriate amount (total volume was 100 ml with purified water.) The above lotion showed astringency and was found to have the effect of restoring the skin to normal after sunburn. That is, after sunburn,
A comparison between the case where the lotion was applied for 3 days and the case where the lotion was not applied was compared. When the lotion was applied, the moisturized state was restored after 3 days, but when the lotion was not applied, the moisturized state was restored after 3 days. Did not recover.

Example 4 and Comparative Example 3 Using the same protein powder as in Example 1, the following lotions were prepared. Protein powder 0.5% Glycerin 2.0% Propylene glycol 2.0% Oleyl alcohol 0.1% Polyoxyethylene sorbitan monolaurate ester (20 EO) 1.5% Polyoxyethylene lauryl ether (20 EO) ) 0.3% ethanol 10.0% Fragrance 0.1% Preservative / UV absorber Appropriate amount (total volume was 100 ml with purified water) Lotion without protein powder from the above formulation (Comparative Example 3)
Was prepared and compared with the lotion of Example 4, a softening effect was recognized in all of the hands with rough skin. However, when the protein powder was added, the skin became shiny and moisturized, whereas when the protein powder was not added, the skin moisture and luster were not sufficiently restored.

Example 5 and Comparative Example 4 The following cream was prepared with the protein powder used in Example 1. Protein powder 2% Stearic acid 1% Stearyl alcohol 5% Reduced lanolin 2% Squalane 5% Octyldodecanol 6% Polyoxyethylene cetyl ether (25EO) 3% Lipophilic monostearate glycerin 2% Propylene glycol 5% Purified 68% water Fragrance / preservative / antioxidant Suitable amount Cream excluding protein powder from the above formulation (Comparative Example 4)
Was prepared and compared with the cream of Example 5, the cream to which the protein powder was added had a greater moisturizing effect than the cream containing no protein powder.

Example 6 The toxicity (cytotoxicity) of the protein powders used in Examples 1 and 2 was assayed by the Neutral Red Bioassay manufactured by Kurashiki Spinning Co., Ltd. None of the protein powders showed toxicity. The outline of the test method is shown below.

1) Preparation of 96-well plate After removing normal human epidermal keratinocytes in secondary culture with trypsin, trypsin activity is stopped with a trypsin neutralizing solution, and the cells are collected. Cells are centrifuged, redispersed in KGM (Epidermal keratinocyte growth medium) and cell counted. A predetermined amount of cells are seeded in the wells of a 96-well plate. The plate is incubated for 3 days at 37 ° C., 5% CO ₂ , saturated steam incubator.

2) Addition of test reagent Fresh K-GM is added to control wells, and test reagents diluted variously with K-GM are added to other wells. Incubate the plate for an additional 2 days.

3) Neutral red (3-amino-7
Ingestion of dimethylamino-2-methylphenazine hydrochloride, MW 288.8) Add neutral red K-GM solution to each well. Incubate the plate for 3 hours. At this time, neutral red is accumulated in lysosomes of living epidermal keratinocytes. Cells with damaged lysosomal or plasma membranes due to test reagents cannot uptake Neutral Red.

4) Washing / fixation, dye extraction, measurement by end point method Dye solution was discarded and fixed with formalin / calcium chloride aqueous solution for a short time to remove unincorporated dye solution, and at the same time adsorb keratinocytes to the plate. Increase. Next, the wells are treated with an acetic acid / ethanol mixed solution to extract the incorporated neutral red. Next, the absorbance of each well, which is proportional to the number of living cells, is measured with a microplate reader at a wavelength of 540 nm. NR50 is calculated by calculating the percentage of the uptake amount of the test reagent with respect to the uptake amount of neutral red in the untreated control (NR50
Is the concentration of the test reagent when the amount of neutral red uptake by the treated cell culture is reduced to 50% with respect to the control culture).

Example 7 2.5 g of animal hair solubilizing factor was suspended in 50 ml of water,
With stirring at 0 ° C, 1 g of succinic anhydride was added 4 times at intervals of 1 hour for succinylation. The pH of the reaction solution is 1NN
It was kept at 6-9 by adding aOH. After the reaction was completed, it was freeze-dried to obtain 8.41 g of a succinylated animal hair solubilizing factor as a white powder. The degree of succinylation was measured by sampling a part of the reaction solution and quantifying the amount of free amino groups by the ninhydrin colorimetric method. The results are shown in Table 3 below.

[0044]

[Table 3]

The solubilities of the above solubilizing factors and succinyl compounds in water are shown in Table 4 below. As is clear from Table 4, the succinylation significantly improves the solubility.

[0046]

[Table 4]

Further, the surface activity of the above-mentioned solubilizing factor and succinyl compound was measured, and the results are shown in Table 5 below.
The evaluation of the surface activity is 0.1% (w / v) of succinyl compound.
3 ml of the aqueous solution and 1 ml of soybean oil were put into a tester and emulsified with a lab disperser (12,000 rpm, 1 minute), and 100 μl of the emulsion was added to 5 m of SDS 0.1% aqueous solution.
After diluting with 1, the turbidity (absorbance at 660 nm) was measured.

[0048]

[Table 5]

Example 8 A lotion composition was prepared according to the following formulation, using the succinyl compound prepared in Example 7 instead of the protein powder and the solubilizing agent in the lotion composition prepared in Example 1. Succinyl compound 3.0% Oleyl alcohol 0.1% Ethanol 10.0% Perfume 0.1% Coloring agent, antioxidants (The total volume was 100 ml with purified water.) The dispersibility was better than that of Example 1, and when applied to the skin, the skin was made to softly retain water and moistened.

Example 9 A protein powder and a solubilizer in the cream prepared in Example 5 were replaced by the succinyl compound prepared in Example 7 in place of the lipophilic glyceryl monostearate to prepare a cream having the following formulation. Succinyl compound 3.0% Stearic acid 1.0% Stearyl alcohol 5.0% Reduced lanolin 2.0% Squalane 5.0% Octyldodecanol 6.0% Polyoxyethylene cetyl ether (25E.O.) 0.5 % Propylene glycol 5.0% Purified water 72.0% Fragrance, antiseptic, antioxidant The preparation of the above cream has better dispersibility than that of Example 5, and both emulsion stability and moisturizing effect are improved. Was.

Example 10 Succinyl compound 5.0% Stearic acid 1.0% Polyoxyethylene cetyl ether (25E.O.) 0.1% Purified water 93.0% Perfume, preservative, antioxidant Properly the above composition Of the emulsified composition of (3-5 kg /
cm ² ), and mixed with liquefied gas (LPG). The same mixture was ejected from a hole having a diameter of 5 mm to foam. When this foam was applied to the hair, it quickly defoamed, and the hair was stylisted to be moist, easy to blend into the hair, non-sticky, and moist.

[0052]

The cosmetic composition containing the animal hair solubilizing factor of the present invention has an excellent effect of replenishing the stratum corneum of the skin with water and a moisturizing component to condition the skin, and also to the epidermis. It is considered physiologically desirable because it contains the same protein as cells. In addition, since lanolin, which is a typical emollient agent, has a close ecological relationship with animal hair, particularly wool, the wool protein and emollient agent contained in the cosmetic composition of the present invention are extremely naturally compatible. be able to. Furthermore, wool protein promotes the proliferation of epidermal cells, and this action has the effect of keeping the skin youthful at all times. The protein is extremely safe without showing toxicity, as is apparent from the assay using the neutral red bioassay (using a test kit commercially available from Kurashiki Spinning Co., Ltd.), which is an alternative method of the Draize test. ..

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Toyoka Nishino 14-5 Shimokitada-machi, Neyagawa-shi, Osaka, Kurashiki Spinning Co., Ltd. Technical Research Institute (72) Shoji Nojima 3-85, Edobashi, Tsu-shi, Mie Kurashiki Spinning Co., Ltd. Tsu Factory (72) Inventor Fumiaki Sakai 14-5 Shimokitada-cho, Neyagawa-shi, Osaka Kurashiki Spinning Co., Ltd. Technical Research Institute

Claims (4)

[Claims] 1. A cosmetic composition containing a solubilizing factor for animal hair. 2. The cosmetic composition according to claim 1, wherein the animal hair is wool. 3. A cosmetic composition containing a solubilizing factor obtained by chemically modifying at least a part of the solubilizing factor according to claim 1. 4. The cosmetic composition according to claim 3, which contains a solubilizing factor having a hydrophilic group introduced therein.