JPH0553458U - Culture vessel - Google Patents

Abstract

(57) [Summary] [Structure] A bottom surface 2 of the main body having a main body 1 and a lid 11.
The opening surface 3 is substantially square, the opening surface 3 is larger than the bottom surface 2, and the height 4a of the main body 1 is equal to the height 4a of the main body 1.
Is shorter than one side length 3a of the opening surface 3 of the main body 1 and
Main body side locking part 6 and lid side locking part 16 for locking the lid 11
Are provided with locking projections 7a, 7b, and 17, respectively, and a gap is formed between the main body 1 and the lid 11 by the locking portion. [Effect] The lid is locked only by pressing it against the main body, and since the bottom of the main body is shallow, it is very easy to work and there is no risk of damaging the plant. Since the culture container is not sealed, sufficient oxygen is supplied to the plant to promote growth. Since the main body portions and the lid portions can be overlapped with each other, they are not bulky when stored. Since the dot-shaped projections that engage with the grid-shaped projections provided on the outside of the bottom surface of the main body are provided on the outside of the top surface of the lid, the culture vessels do not easily slip off when the culture vessels are stacked.

Description

[Detailed description of the device]

[0001]

[Industrial application]

 The present invention relates to a culture vessel used for tissue culture of plants.

[0002]

[Prior Art]

 Conventionally, for tissue culture of plants, as shown in FIG. 4, a generally cylindrical general-purpose molded bottle composed of a main body 31 and a lid 32 is used as a culture container. This culture container is manufactured by injection molding a hard / transparent plastic such as a polycarbonate resin.

A male screw 33 is formed in the opening of the main body 31 of the culture container, while a female screw 34 corresponding to the male screw 33 is formed inside the lid 32, and the lid 32 is screwed to the main body 31. By doing so, the culture container is sealed. The diameter of the opening of the body 31 where the male screw 33 is formed is smaller than the diameter of the body 31 itself.

When planting a tissue to be tissue-cultured (hereinafter referred to as a plant) in this culture container, first, an agar medium containing nutrients necessary for the growth of the plant is prepared inside the main body 31. Then, heat-dissolve, measure and dispense, heat-sterilize in, for example, an autoclave, plant the plant, and then attach the lid 32 to the culture shelf, culture facility or culture facility where the predetermined conditions are set. Culture side by side.

[0005]

[Problems to be solved by the device]

 However, since the above-mentioned conventional culture vessels have a circular bottom surface, for example, when accommodating a large number of culture vessels in one culture facility, they are not arranged side by side without a gap and a dead space is provided inside the culture facility. Occurs. In addition, when taking out plants that have been cultivated inside the culture container by, for example, splicing work for the purpose of increasing the number of plants, it takes time to open and close because the lid is a screw, and the bottom of the main body is Since it is deep, it is very difficult to take out and the workability is poor, the risk of contamination by bacteria is increased, and the plants may be damaged by tweezers or the narrowed opening of the main body.

Further, since the culture container is hermetically sealed, the inside becomes over-humidified, and moreover, sufficient oxygen is not supplied, the plant body grows fragile, and water droplets adhere to the inside of the main body and become cloudy. It becomes easier and it is difficult to observe the growth of plants from the outside.

[0007] Furthermore, this culture container cannot be processed in large quantities at a time when heat sterilized in an autoclave because the main body and the main body and the lid and the lid cannot be overlapped, and the culture container is bulky and large. A space is required, and when the culture vessel is washed after culturing the plant, the bottom of the body is deep, making it difficult to wash the inside of the body.

[0008]

[Means for Solving the Problems]

 In order to solve the above-mentioned problems, the culture container of the present invention is a culture container used for tissue culture of plants, and has a main body and a lid, and the bottom surface and the opening surface of the main body are substantially square. In addition, the opening surface is larger than the bottom surface, the height of the main body is shorter than the length of one side of the opening surface of the main body, and each of the main body and the lid that locks the main body and the lid is engaged. Each stop surface is provided with a locking means, and the locking means forms a gap between the main body and the lid.

Further, the culture container for tissue culture of plants has a main body and a lid, and the point-like projections engaging with the grid-like projections provided on the outside of the bottom surface of the main body are the lids. It is characterized by being installed outside the top surface.

Further, a culture vessel for tissue culture of a plant, which has a main body and a lid, and the main body and the main body described above and the lid and the lid can be overlapped with each other, and It is characterized in that a scale for measuring the amount of medium is formed on the outside of the side surface of the main body.

[0011]

[Advantages] With the above configuration, since the bottom surface of the main body is substantially square, when, for example, a large number of culture vessels are housed in one culture facility, they are arranged with almost no space, and a dead space is provided inside the culture facility. Does not occur. Also, when taking out a plant that has been cultivated in the culture container by, for example, a splicing operation for the purpose of increasing the number of planted objects, the lid is locked by simply pressing it lightly on the main body, so the lid can be attached and detached. It is easy to take out, and because the bottom of the main body is smaller than the opening, it is very easy to take out, improving workability, and there is no risk of damaging plants with tweezers.

In addition, since a gap is formed between the main body and the lid, the inside of the culture container does not become a sealed state and proper air permeability is maintained, so that sufficient oxygen is supplied to the plant body. In addition, the growth of plants is promoted, and water droplets are less likely to adhere to the inside of the main body so that it does not become cloudy and the growth of plants can be easily observed from the outside.

Furthermore, this culture container is not bulky when it is stored because the main body part and the main body part or the lid part and the lid part can be overlapped, and a small storage space is required, and the culture container is washed after culturing the plant body. When doing this, the inside of the body is easy to wash because the depth of the body is shorter than the length of one side of the opening surface.

Moreover, since a scale indicating the amount of the agar medium is formed on the outside of the side surface of the main body, it is not necessary to weigh the agar medium in another container, and a grid provided on the outside of the bottom surface of the main body. Since the point projections that engage with the projections are provided outside the top surface of the lid, the culture vessels do not easily slip off when the culture vessels are stacked.

[0015]

【Example】

 An embodiment of the present invention will be described below with reference to FIGS.

As shown in FIG. 1, the culture container according to the present invention comprises a main body 1 and a lid 11. As shown in FIG. 2, the main body 1 is made by injection molding a hard and transparent heat-resistant plastic such as a polycarbonate resin. Since the bottom surface 2 and the opening surface 3 of the main body portion 1 are substantially square and the opening surface 3 is wider than the bottom surface 2, the side surfaces 4 ... Of the main body portion 1 have a substantially inverted trapezoidal shape and face the opening surface 3. Is inclined to spread outwards according to. In addition, since the height 4a of the side surface 4 of the main body 1 is set to be smaller than the length 3a of one side of the opening surface 3, the main body 1 is a container with a shallow bottom relative to the size of the opening surface 3. In addition, since each side 5a of the bottom portion of the inner surface 5 of the main body 1 is curved, and as will be described later, various scales and the like are all provided outside the main body 1, The inner surface 5 is perfectly smooth.

Outside the vicinity of the opening surface 3 of the main body portion 1, when the lid portion 11 is pressed against the main body portion 1, main body side locking portions 6 that lock the lid portion 11 are perpendicular to the bottom surface 2. The main body side locking portion 6 is provided with two locking projections 7a, 7b parallel to each side of the opening surface 3. A method of locking the lid side locking portion 16 of the lid portion 11 with the locking projection 17 by 7b will be described later.

In addition, in two of the four side surfaces 4 of the main body 1, the amount of agar to be poured into the main body 1 at the time of producing an agar medium to be described later can be seen at a glance. A quantity scale 8 is provided.

On the bottom surface 2, after the agar medium is prepared inside the main body 1, a plurality of plant bodies (hereinafter referred to as plant bodies) to be tissue-cultured can be arranged in an orderly manner on the agar medium. Is provided with grid-like projections 9 of a grid. The culture medium scale 8 and the grid-like projections 9 are all provided outside the main body 1.

Next, as shown in FIG. 2, the lid portion 11 is made by injection-molding a heat-resistant hard / transparent plastic of the same quality as the main body portion 1, such as polycarbonate resin. .. Since the top surface 12 and the opening surface 13 of the lid portion 11 are substantially square and the opening surface 13 is wider than the top surface 12, the side surfaces 14 of the lid portion 11 are substantially trapezoidal and the opening portion 13 is formed. It is inclined so that it spreads outward as it goes to. Further, each side 15a of the bottom of the inner surface 15 of the lid 11 is curved, and the inner surface 15 is completely smoothed.

Outside the opening surface 13 of the lid portion 11, lid-side locking portions 16 that lock the lid portion 11 with the main body portion 1 when the lid portion 11 is pressed against the main body portion 1 are topped. The locking projections 17 are provided perpendicularly to the surface 12, and the lid side locking portions 16 are provided with locking projections 17 parallel to the respective sides of the opening surface 13. A method of locking the main body side locking portion 6 of the main body portion 1 with the locking projections 7a and 7b by means of will be described later.

The size of the top surface 12 of the lid 11 is the same as the size of the bottom surface 2 of the main body 1, and the size of the opening surface 13 of the lid 11 is the size of the opening surface of the main body 1. It is almost equal to the size of 3 plus the thickness of the main body 1 and the height of the locking projections 17. Therefore, when the lid portion 11 is locked to the main body portion 1, a gap approximately equal to the height of the locking projection 17 is formed between the lid side locking portion 16 and the main body side locking portion 6. It is becoming like this.

Further, one of the four side surfaces 14 of the lid portion 11 has a sticking portion 18 for sticking a label in which the kind of plant body planted on the agar medium inside the main body portion 1, culture conditions, etc. are attached. The sticking portion 18 is provided with fine irregularities on its surface, so that the label can be easily peeled off.

Outside the top surface 12, a grid pattern is provided on the outside of the bottom surface 2 of the main body 1 in order to prevent the culture vessels from easily slipping down due to vibration when the culture vessels are stacked. Point-like projections 19 ... For engaging the child-like projections 9 are provided. The dot-like projections 19 are arranged so as to match the corners 9a inside the grid of the grid-like projections 9 of the main body 1 when the culture vessels are stacked.

Next, the engagement between the engagement protrusions 7 a and 7 b provided on the body side engagement portion 6 of the body portion 1 and the engagement protrusion 17 provided on the lid side engagement portion 16 of the lid portion 11. The stop will be described below with reference to FIGS. 1 to 3.

First, the locking projections 7 a and 7 b provided on the main body side locking section 6 of the main body section 1 and the locking projections 17 provided on the lid side locking section 16 of the lid section 11 are locked. Is performed by lightly pressing the lid 11 against the main body 1 from above. At this time, the main body side locking portion 6 of the main body portion 1 and the lid side locking portion 16 of the lid portion 11 are slightly distorted, and the locking projections 17 provided on the lid side locking portion 16 of the lid portion 11 are provided. Is pressed down while coming into contact with the locking projection 7a provided on the main body side locking portion 6 of the main body 1, crossing over the locking projection 7a and hitting the locking projection 7b to stop.

That is, the lid portion 11 covers the main body portion 1 and is locked. At this time, a gap of several mm is formed between the edges 1a of the opening surface 3 of the main body 1 and the step 11a of the opening surface 13 of the lid 11 so that air can satisfactorily flow therethrough. ing.

When the lid part 11 is further pressed against the main body part 1, the locking projections 17 provided on the lid side locking part 16 of the lid part 11 are attached to the main body side locking part 6 of the main body part 1. The lid 11 is pushed down while coming into contact with the provided locking projection 7b, crosses over the locking projection 7b, and the lid 11 is locked so as to cover the main body 1 deeper than before.

In this case, the edge 1a of the opening surface 3 of the main body 1 and the step 11a of the opening surface 13 of the lid 11 are in contact with each other so that the main body 1 does not fit into the lid 11 more deeply than necessary. A small gap is formed between the main body 1 and the lid 11 so that air can flow appropriately.

When the lid portion 11 is removed from the body portion 1, the side surface 4 of the body portion 1 is pressed and the lid portion 11 is pulled upward, so that the body side locking portion 6 and the lid portion of the body portion 1 The lid-side locking portions 16 of 11 are slightly distorted, and the locking protrusions 7a and 7b provided on the body-side locking portion 6 of the body 1 and the lid-side locking portions 16 of the lid portion 11 are provided. The locking projection 17 is unlocked, and the lid 11 can be removed from the main body 1.

An example of a method for cultivating a plant using the culture container having the above configuration will be shown below.

First, a culture medium and an agar medium such as LS (Lynsmeyer and Skoog) agar medium containing nutrients and plant hormones necessary for plant growth are sterilized by heating in an autoclave, for example. At this time, the culture containers can be put into a heat-resistant autoclave bag or the like by stacking the main body parts 1 and the lid parts 11 on top of each other, and can be efficiently heat-sterilized in the autoclave.

Next, after agar medium is dispensed into the main body portion 1 in a predetermined amount in a sterile environment such as a clean bench, the lid portion 11 is lightly pressed against the main body portion 1 from above, and the main body portion of the main body portion 1 is pressed. The locking projections 7a and 7b provided on the side locking portion 6 and the locking projections 17 provided on the lid side locking portion 16 of the lid portion 11 are locked, so that the main body portion 1 and the lid portion 11 are separated from each other. Lock it to prevent dust from entering the agar medium.

At this time, since the culture container is provided with a medium amount scale 8 and a separate container is not required for measuring the agar medium, it is easy to dispense the agar medium, and the main body side When the locking projections 7a and 7b provided on the locking portion 6 and the locking projections 17 provided on the lid side locking portion 16 are locked, a few pieces are provided between the main body 1 and the lid 11. Since there is a gap of mm, air flow is good, evaporation of water in the agar medium and coagulation of the agar medium are promoted, and water droplets do not adhere to the inside of the culture vessel.

Next, the lid part 11 is removed from the main body part 1 and a plant such as tobacco is planted on the agar medium. At this time, since the bottom of the main body 1 is shallow, the work is easy to perform, and since the grid-shaped projections 9 are provided on the outside of the bottom surface 2 of the main body 1, the grid-shaped projections 9 are used as a guide for one culture container. Multiple plants can be planted in an orderly manner.

After that, the lid portion 11 is pressed again against the main body portion 1 so that the edge 1 a of the opening surface 3 of the main body portion 1 and the step portion 11 a of the opening surface 13 of the lid portion 11 come into contact with each other. The main body 1 is deeply inserted into the lid 11 to prevent unnecessary evaporation of water in the agar medium and prevent dust and the like from entering the agar medium. At this time, since the culture container is not hermetically sealed and there is a slight gap between the main body 1 and the lid 11, the culture container is kept adequately airtight and airtight, so that the culture container is sufficiently sealed to the plant body. Oxygen is supplied to promote the growth of plants. In addition, since water droplets that evaporate from the agar medium do not easily adhere to the inside of the culture container, it does not become cloudy, and the growth status of the plant can be easily observed from the outside.

Then, a large number of the plants thus treated are placed in a culture facility or the like set to predetermined conditions and cultured. In this case, since the bottom surface 2 of the main body 1 of the culture container is substantially square, the culture container is lined up in the culture equipment with almost no space, and no dead space is generated inside the culture equipment. Further, since the dot-like projections 19 provided on the outside of the top surface 12 of the lid 11 and the lattice-like projections 9 provided on the outside of the bottom surface 2 of the main body 1 engage with each other, when the culture vessels are stacked. The upper culture vessel does not slip off easily. Then, even if the plant grows and becomes taller, it is only necessary to replace the lid 11 with a tall one according to the growth of the plant, and it is not necessary to replant the plant in another culture container. ..

After culturing in a culturing facility or the like for a certain period of time, the plants in the culture container are taken out with tweezers or the like, and transplanted for the purpose of increasing the number of plants, for example. At this time as well, since the main body 1 has a shallow bottom, the work is easy to perform, and the plant can be easily taken out from the main body 1 without damaging the plant. Alternatively, the shoots can be divided without removing the plant from the main body 1.

In addition, when the culture vessel is washed after the cultivation of the plant is completed, the inner surface 5 of the main body 1 has no dents or the like, so that the agar medium can be easily removed, and when the culture medium is immersed in a detergent solution, Since the main body parts 1 and the lid parts 11 can be overlapped with each other, they can be efficiently immersed in the cleaning liquid. At this time, if the label has a normal adhesiveness, the label is easily peeled off from the sticking portion 18 due to the fine unevenness of the sticking portion 18 of the lid 11.

Since both the main body 1 and the lid 11 of this culture vessel have few irregularities, and the bottom of the main body 1 is shallow, it is possible to sufficiently remove dirt even if it is automatically washed by an automatic washing machine, for example. it can.

As described above, since the bottom surface of the main body of the culture container having the above-mentioned configuration is substantially square, for example, the culture vessels can be arranged in the culture equipment with almost no space therebetween, and the lid can be lightly pressed against the main body. Since the lid is easily attached and detached, the bottom of the main body is smaller than the opening, so that it is very easy to take out, the workability is improved, and the plant is not damaged. In addition, since the scale showing the amount of agar medium is formed on the outside of the side surface of the main body, it is not necessary to weigh the agar medium in another container, and the grid-shaped projections on the outside of the bottom of the main body Since the engaging point projections are provided on the outside of the top surface of the lid, the culture vessels do not easily slip off when the culture vessels are stacked.

Further, since the inside of the culture container is not hermetically sealed and proper air permeability is maintained, sufficient oxygen is supplied to the planted matter, and the growth of the plant body is promoted. Further, since the main body and the main body or the lid and the lid can be overlapped with each other, they are not bulky when they are stored, and a small storage space is required, and the structure is very easy to wash when cleaning.

[0043]

[Effect of the device]

 As described above, the culture container of the present invention has the main body and the lid, the bottom surface and the opening surface of the main body portion are substantially square, and the opening surface is larger than the bottom surface. The height is shorter than the length of one side of the opening surface of the main body, and locking means are provided on the respective locking surfaces of the main body and the lid for locking the main body and the lid. The means forms a gap between the main body and the lid.

In addition, the configuration is such that a dot-shaped projection that has a main body and a lid and that engages with a lattice-shaped projection that is provided outside the bottom surface of the main body is provided outside the top surface of the lid.

Further, it has a main body part and a lid part, and the main body part and the main body part can be overlapped with each other, and the lid part and the lid part can be overlapped with each other, and a scale for measuring the amount of culture medium outside the side surface of the main body part. Is formed.

Therefore, since the bottom surface of the main body is substantially square, for example, when a large number of culture vessels are housed in one culture facility, they are arranged with almost no space, and no dead space is generated inside the culture facility. .. Also, when the plant body cultured in the culture container is taken out by splicing work, etc., the lid part is locked by simply pressing it against the main body part, so that the lid part can be easily attached and detached. Since the bottom surface is smaller than the opening surface, it is very easy to take out, the workability is improved, and there is no risk of damaging the plant body with tweezers.

In addition, since a gap is formed between the main body and the lid, the inside of the culture container does not become a sealed state, and proper ventilation is maintained, so that sufficient oxygen is supplied to the plant. In addition, the growth of plants is promoted, and water droplets are less likely to adhere to the inside of the main body so that it does not become cloudy and the growth of plants can be easily observed from the outside.

Further, this culture container is not bulky when sterilized by an autoclave or the like for storage because the main body part and the main body part or the lid part and the lid part can be overlapped, and it is a small storage space. In addition, a small water tank can be used when the culture vessel is immersed in a detergent solution to wash the culture vessel after culturing the plant. Further, during cleaning, the depth of the main body is shorter than the length of one side of the opening surface, and since there is no dent on the inner surface, the inside of the main body can be easily washed.

Moreover, since a scale indicating the amount of the agar medium is formed on the outside of the side surface of the main body, it is not necessary to weigh the agar medium in another container, and a grid provided on the outside of the bottom surface of the main body. Since the point-like protrusions that engage with the protrusions are provided on the outside of the top surface of the lid, there is an effect that the culture vessels do not easily slip off when the culture vessels are stacked.

[Brief description of drawings]

FIG. 1 is a schematic view of a culture container according to an embodiment of the present invention.

FIG. 2 is a sketch drawing of a main body portion and a lid portion of the culture container.

FIG. 3 is a schematic cross-sectional view showing a locked state of a main body portion and a lid portion of the culture container.

FIG. 4 is a sketch drawing of a conventional culture container.

[Explanation of symbols]

 1 Main Body 2 Bottom Surface 3 Opening Surface 4 Side Surface 6 Main Body Side Locking Part 7a Locking Protrusion 8 Medium Volume Scale 9 Lattice-like Projection 11 Lid 12 Top Surface 13 Opening Surface 16 Lid Side Locking Part 17 Locking Protrusion 19 point projections

Claims (3)

[Scope of utility model registration request] 1. A culture vessel used for tissue culture of plants, comprising a main body and a lid, wherein the bottom surface and the opening surface of the main body are substantially square, and the opening surface is larger than the bottom surface.
Further, the height of the main body is shorter than the length of one side of the opening surface of the main body, and locking means are respectively provided on the locking surfaces of the main body and the lid for locking the main body and the lid. A culture container provided, wherein a gap is formed between the main body portion and the lid portion by the locking means. 2. A culture vessel used for tissue culture of plants, comprising a main body and a lid, and the lid-like projections are point-like projections engaging with lattice-like projections provided on the outside of the bottom surface of the main body. The culture container according to claim 1, wherein the culture container is provided outside the top surface. 3. A culture vessel used for tissue culture of a plant, comprising a main body and a lid, wherein the main body and the main body and the lid and the lid can be overlapped with each other, and the main body The culture container according to claim 1, wherein a scale for measuring the amount of the medium is formed on the outside of the side surface of the portion.