JPH04718Y2 - - Google Patents

Description

[Detailed description of the invention] [Purpose of the invention] (Field of industrial application) The present invention relates to a culture container used for culturing plants, etc.

(Prior Art) In recent years, so-called culture, which divides and multiplies microorganisms, tissue cells, etc., has been actively carried out, and in particular, cell culture called tissue culture is the cultivation of cell groups or tissue pieces. This culture requires a medium, and various containers are used to contain it.

An example of a container for such a purpose is a transparent glass Erlenmeyer flask as shown in FIG. 12, in which culture medium b is placed in flask a and then tissue pieces are placed thereon. In this case, in order to prevent bacteria from entering flask a,
A type covered with aluminum foil c as shown in Fig. 2A, a cotton plug d as shown in Fig. 12B, and a silicone plug e as shown in Fig. 12C are used.

In addition, as shown in Fig. 13, a rectangular deep-bottomed box f made of thick-walled plastic, etc., with a shallow lid g.
In some cases, a culture medium is placed in the box f, and a tissue piece is placed in the medium for cultivation.

(Problems to be Solved by the Invention) However, the Erlenmeyer flask a described above has the following drawbacks. (1) When dispensing the culture medium, the container has a small mouth and a deep bottom, so the surface of the culture medium is far away, and it is difficult to insert it deep into the container using tweezers etc. in order to reach the surface. If you fail to do so, the culture medium will scatter and easily stain the inside of the container.

(2) When placing small plant pieces on a bed, it is difficult to align them in the same direction, and work efficiency is extremely poor.

(3) When transplanting a culture grown in a container to another container, the culture is likely to be damaged because it must be picked up from the bottom of the deep container.

(4) Excessive moisture tends to cause morphological abnormalities, delayed growth, and reduced rooting rate.

In addition, in the case of a lid made of aluminum foil, there is also the problem that if the culture solution comes into contact with the aluminum foil, it will die from the contact area.

If the container shown in Fig. 13 is used in place of the Erlenmeyer flask a, it may not be as difficult to use as the Erlenmeyer flask, but since it is a deep-bottomed container, the above (1) to (3) will be met. The same problem as above cannot be avoided, and the lid g is fitted onto the top of the box f that constitutes the container, and the fitting part has a double structure h, and this part is exposed to direct sunlight necessary for growing plants. It has the disadvantage that the transmission of light 1 is significantly reduced, and the light-transmitting area as a culture container is limited.

On the other hand, in any of the above culture vessels, it is difficult to observe the details with the naked eye when observing the culture status, germination and growth status, and contamination status of the culture inside, and the culture vessels are stacked in multiple stages. It becomes even more difficult to see the inside of the device if it is managed by a computer.

Furthermore, in order to provide a plug for providing ventilation to such a container, conventionally, a hole for ventilation is made in the lid of the container, and a ventilation hole is filled into the hole to provide ventilation to the inside of the container. However, depending on the material of this vent plug, when steam bacteria enter, the plug absorbs moisture and contracts.
There was a problem that not only did it lose its function as an aeration plug, but also that moisture entered the container and had an adverse effect on the plant cells.

In view of this, the present invention has been designed to provide a culture container that allows for easy placement of cultured materials such as various plant pieces, and allows for accurate internal observation even when the culture containers are stacked and managed. It was made for the purpose of providing.

[Structure of the idea]

(Means for Solving the Problems) As a means for solving the problems of the above-mentioned prior art, the present invention provides a culture container having a container body and a lid for closing an upper opening of the container body. and a lid is formed of a thin plastic material having light transmittance and heat resistance, the container body having a bottom portion for accommodating the culture medium and a shallow side wall, and a lid joining portion is formed at the upper end of the side wall, The lid has a top plate and a vertically elongated side wall, and a main body connecting portion that can be connected to the lid connecting portion is formed at the lower end of the side wall, and a lens is provided on the side wall of the lid, and the interior can be seen from the side through the lens. It is characterized by being able to be viewed magnified.

(Function) With the above configuration, if a culture medium is housed in the container body, a piece of tissue such as a plant is placed therein, and the lid is fitted, the vertically long lid has a double structure part that blocks incident light. Since there are no holes, light can be easily transmitted into the container, and if you look inside from the circumferential side of the lid, the lens formed on the side wall of the lid will magnify the state of the culture inside the container body and observe the details. be able to.

(Embodiments) The present invention will be described below with reference to embodiments shown in FIGS. 1 to 11.

The culture container 1 according to the present invention has a substantially cylindrical shape and consists of a container body 2 and a lid 3 that is fitted over the upper opening of the container body 2, both of which are made of transparent plastic, such as polycarbonate. It is made of a heat-resistant plastic material that can withstand heat sterilization treatments such as

The container body 2 is vessel-shaped, consisting of a circular bottom 4 and a shallow side wall 5 standing up around the bottom, and a lid connecting portion 6 is formed at the upper end of the side wall 5.

The lid 3 consists of a circular top plate 7 and a deep side wall 8 that is vertically disposed around the top plate 7, and the height of this side wall 8 is approximately twice the height of the container body 2. It is said that the dimensions are . A main body coupling part 9 is formed at the lower end of the side wall 8 and is externally fitted onto the lid coupling part 6 at the upper end of the side wall of the main body 2 as tightly as possible. In the illustrated embodiment, the container main body 2 and the lid 3 are connected to each other by means of screw connection, but this connection is possible by force fitting or by adding a bayonet connection means. Optional.

Around the top plate 7 of the lid 3, there is a bottom 4 of the container body 2.
A projecting edge 11 is formed in which the stepped portion 10 formed on the lower surface periphery of the container engages and the container body 2 is stacked without shifting.
A is a tapered surface that widens upward.

A lens 12 is provided on the side wall 8 of the lid 3 to provide an enlarged view of the inside of the container body 2.

The structure of this lens 12 is shown in FIGS. 2 to 7.
An example is shown in the figure. In the embodiment shown in FIG. 2, a plurality of convex lenses 12, 12, . A split mold is used. The number of convex lenses 12, 12... to be installed may vary depending on the size of the culture container, such as 4 in the circumferential direction, 3 at equal intervals as shown in the horizontal cross section in Figure 3, or 2 at symmetrical positions. be selected as appropriate.

In the embodiment shown in FIGS. 4 and 5, plano-convex lenses 12', 12', . Yes, in this example there are four plano-convex lenses 1
2', 12'... are formed by molding. In this case as well, the number of installations is arbitrary, such as installing two at symmetrical positions, and in this example, a cutting die can be used.

Furthermore, Figs. 6 and 7 show Fresnel lens 1.
FIG. 6 shows an example in which a Fresnel lens 12'' formed in a sheet shape is wrapped around the outer surface of the side wall 18 of the lid 3 and bonded. Also the 7th
In the figure, it is glued along the inner surface of the side wall 8 of the lid 3. In addition, when molding the lid 3, a Fresnel lens may be integrally molded on a part of the outer surface or inner surface of the side wall 8 using a split mold.

The above ranges 12, 12', 12'' are designed to obtain a virtual image as a<f, where the distance to the object to be observed is a, the distance to the image is b, and the focal length of the lens is f, and the magnification is X=b. /a is preferably in the range of 1.3<X<2.0.

FIG. 8 shows an embodiment in which a ventilation plug is provided in the lid 3, and a plug storage recess 13 is integrally formed near one side of the top plate 7 of the lid 3, as shown in the cross section of the part in FIG. has been done. This plug recess 13 has an inner diameter of 8 to 9 m/m and a depth of about 10 m/m, and has a cylindrical shape on the inner surface of the side wall 8 on the lower surface side of the top plate 7 of the lid 3. Peripheral wall 14 formed in a container shape
The upper part is open to the upper surface of the top plate 7, and the lower bottom part 15 has a ventilation hole 16.
is drilled.

A jetty 17 that is lower in height than the projecting edge 11 on the peripheral edge of the top plate 7 is provided around the opening edge of the upper opening of the stopper storage recess 13, and this jetty 17 engages with the projecting edge 13. The height is such that the bottom portions 4 of the container bodies 2 that are stacked on top of each other just touch or do not touch each other.

A cylindrical ventilation plug 18 is inserted into the plug storage recess 13 as tightly as possible. This vent plug 1
8 is made of a material that does not shrink even when it absorbs moisture not only during dry sterilization but also during wet (steam) sterilization. The material is, for example, a non-woven fabric rolled up by winding, folding, aggregating, etc. with the fiber direction in the axial direction, and then wrapped in Japanese paper or the like to form a cylindrical shape, with a thickness of 8 to 9 φ and a length of 11 m. /m.

In FIG. 2, reference numeral 19 indicates a culture medium.

Next, the operation of the above embodiment will be explained.

A culture medium 19 is contained in the container body 2, and tissue pieces such as plants are placed therein. When placing the container on the floor, since the container body 2 has a shallow structure, the culture medium 19 is located close to the open upper surface of the container body 2, making it easy to place the container on the floor and to align the orientation.

Next, the main body connecting part 9 of the lid 3 is screwed into the lid connecting part 6 of the container body 2, and the culture container 1 is formed into a vertically long cylindrical shape as shown in FIGS. 1, 3, and 8.

When stacking empty culture containers 1 or after adding culture material, if the culture containers 1 and 1 are stacked on top of each other as shown in FIG. The culture containers 1 can be stacked in an orderly manner so as not to shift by engaging with the protruding edge 11 protruding from the periphery of the lid 3 of the culture container 1.

The container 1 with the lid 3 joined in this way has excellent light transmittance, and since the joining part 9 between the lid 3 and the main body 2 is also located at a low position near the container main body 2, direct light from above will not penetrate the culture medium. There is no obstruction to the
The lens 1 provided on the side of the lid 3 can be used to observe the entire surface of the container and to observe the inside of the container.
By looking inside the container body 2 through 2, 12', and 12'', you can enlarge the culture and observe it.Even if the culture containers are stacked in multiple tiers, you can easily make an enlarged observation to accurately check the growth status, etc. can be grasped.

After a certain period of time, when removing the lid 3 from the container body 2 and transplanting the culture grown in the container 1 to another container, the culture may be damaged because it is close to the upper end surface of the container body 2. You can take it out without any trouble.

Furthermore, the culture container 1 in the embodiment shown in FIG.
The vent plug 18 whose inside is fitted into the plug storage recess 13 of the lid 3 and the lower bottom 1 of the plug storage recess 13
It is placed in a state where it communicates with the outside air through the ventilation hole 16 of No. 5, and aerobic culture can be carried out without any problems.

On the other hand, the ventilation plug 18 of the lid 3 of the culture container 1 is highly heat resistant and does not shrink even when it absorbs water, so it does not lose its ventilation function and does not come loose and fall off. Since the exposed upper part of the vent plug 18 is protected by the jetty 17 around the opening of the storage recess 13, there is no risk of it being crushed, and moreover, the moisture accumulated on the top surface of the lid 3 flows into the plug storage recess 13 by the jetty 17. This prevents water from entering the culture container 1.

In the illustrated embodiment, the container body 2 and the lid 3 are
Although the case where the cross section is circular is shown, it goes without saying that this may be polygonal.

[Effect of idea]

As explained above, the culture container according to the present invention is
The container body and lid are made of thin transparent plastic material with good light transmittance, and the container body has a shallow bottom and the lid has a deep bottom, and since both can be combined, it is possible to order the culture medium in the container body and to transfer the culture medium. Planting various cultures and transplanting to other containers can be done extremely easily by removing the lid.The lid also has excellent light transparency, allowing direct light to be received throughout the container and making it easy to observe the entire inside of the container. can be done.

Even when the container body and lid are combined, the ventilation conditions are improved because the joint is close to the culture, resulting in faster CO ₂ absorption and superior plant growth. Not only that, but a lens is provided on the side of the lid, which allows you to magnify and observe changes in the culture inside the container body, making it possible to accurately grasp the situation. Furthermore, even if the culture containers are stacked in multiple tiers, the lens on the side of the lid makes it possible to observe the inside without disrupting the stacked state, which is efficient.

In addition, as in claim 2, a stopper storage recess is formed in the top plate of the lid of the culture container, and a material (for example, nonwoven fabric) that is highly heat resistant and does not shrink even when water is absorbed is placed in the recess to control the fiber direction. Since the aeration plug formed in a cylindrical shape in the longitudinal direction is fitted, the culture container can be steam sterilized while ensuring air permeability inside the culture container.

Furthermore, if a jetty is provided at the periphery of the opening of the stopper storage recess as in claim 3, moisture accumulated on the top plate of the lid will not flow into the stopper storage recess.
This prevents water from entering the culture vessels, prevents contamination of plant cells, etc., allows culture vessels to be stacked easily, and is easy to handle when handling the culture vessels. Since there is no direction, it can be adapted to robotization and automatic porting in the future.

If a non-woven fabric is selected as the material for the vent plug, and the non-woven fabric is formed into a cylindrical shape with the fiber direction oriented in the longitudinal direction, it can be used instead of the conventionally used silicone-based or ome cotton-based vent plugs. It is extremely inexpensive (less than 1/10) compared to other materials, and shows almost no shrinkage even after upper air sterilization, making it the most suitable material for the vent plug used in the present invention. In this case, the length of the ventilation plug is preferably the sum of the depth of the plug storage recess and the height of the jetty.

[Brief explanation of the drawing]

FIG. 1 is a perspective view showing an embodiment of the culture container according to the present invention, FIGS. 2A and B are longitudinal cross-sectional views of the lid and the container body taken along the - line in FIG. 1, and FIG. 3 is a lens on the lid. 4 is a perspective view showing another embodiment, and FIG. 5 is a horizontal sectional view when three pieces are used.
6 and 7 are views corresponding to FIG. 5 of the lid showing an embodiment using a Fresnel lens, FIG. 8 is a perspective view of another embodiment of the same, and FIG. 9 10 is a plan view of the same, FIG. 11 is an explanatory diagram showing the stacked state of culture vessels according to the present invention, and FIG. 12 is a conventional Erlenmeyer flask-shaped glass culture vessel. 13 is a perspective view of a container, in which A is a stopper made of aluminum foil, B is a cotton plug, C is a silicone plug, and FIG. 13 is a perspective view of a conventional culture container made of plastic. 1... Culture container, 2... Container body, 3... Lid,
4... Bottom, 5, 8... Side wall, 7... Top plate, 11
...Protrusion, 12, 12', 12''...Lens, 13
...Plug storage recess, 16...Vent hole, 17... Jetty, 18...Vent plug.

Claims (1)

[Claims for Utility Model Registration] 1. A culture container that has a container body and a lid that closes the upper opening of the container body, wherein the container body and the lid are made of a thin plastic material that is transparent to light and has heat resistance. The container body has a bottom portion for accommodating a culture medium and a shallow side wall, and a lid connecting portion is formed at the upper end of the side wall, and the lid has a top plate and a vertically elongated side wall, and the lid has a top plate and a vertical side wall. forming a main body coupling part that can be coupled to the lid coupling part at a lower end thereof, and providing a lens on a side wall of the lid;
A culture container characterized in that the interior can be viewed in an enlarged manner from the side through this lens. 2. The culture vessel according to claim 1, wherein a stopper housing recess having a ventilation hole is provided in the top plate of the lid, and a ventilation stopper made of a material that does not shrink when subjected to wet heat sterilization treatment is packed in the recess. . 3. A projecting edge into which the bottom of the container body can fit is formed on the upper peripheral edge of the top plate of the lid, so that containers can be stacked freely, and a peripheral edge of the upper opening of the stopper storage recess is formed with a height higher than the projecting edge. 3. The culture container according to claim 2, further comprising a low jetty.