JP2589295Y2 - Microbial testing tools - Google Patents

Description

[Detailed description of the invention]

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device for testing microorganisms used in foods and the like.

[0002]

2. Description of the Related Art For microbial testing, for example, Japanese Utility Model Laid-Open No.
As disclosed in JP-A-99-199, JP-A-63-214176, a method of preparing an agar medium containing various components in a petri dish and inoculating a test liquid prepared from a test material on the agar medium is generally performed. However, in this method, the test material is indirectly examined, and preparation of the test solution is troublesome. In the 1970s, a stamp-type bacteria test device was developed in which the agar medium swelled from the edge of a rigid synthetic resin container, and microbes could be easily obtained simply by pressing the surface of the agar medium against the surface of the test material. Inspection is now possible.

[0003]

[Problems to be Solved by the Invention] However, in the food field, the test material often has a convex curved surface, and when the agar medium is pressed against the test material, rubbing occurs on the contact surface, and the test material adheres to the test material. I can't find out exactly how many bacteria
There has been a problem that cracks are generated on the surface of the agar medium due to an external impact, and the agar medium is easily peeled from the container.

An object of the present invention is to solve the above-mentioned conventional problems and to provide a microbial testing tool in which the surface of the agar medium hardly cracks and the agar medium hardly peels off.

[0005]

SUMMARY OF THE INVENTION The present invention comprises a container body and a lid made of a flexible synthetic resin, and has a folded edge extending outwardly around the opening of the container body. Characterized in that a flexible perforated sheet is enclosed in an agar medium filled in the agar medium.

[0006] The flexible synthetic resin used in the device for testing microorganisms of the present invention is not particularly limited, but it is preferable to use a transparent resin so that the growth state of the bacteria can be visually recognized, for example, polystyrene, polypropylene, or the like. Examples include polyvinyl chloride and polyethylene terephthalate.
Further, in order to improve the impact resistance, a rubber component such as butadiene may be blended.

[0007] The thickness and the forming method of the microorganism testing device are not particularly limited, but it is preferable to form the sheet having a thickness of 0.2 to 0.6 mm by vacuum forming or press forming.

[0008] It is preferable that the surface of the microbe inspection tool is roughened so that characters and symbols can be directly written with a writing instrument such as a pencil.

The bottom surface of the container-shaped main body of the device for testing microorganisms is preferably formed to have a convex curved surface which curves upward in order to make the thickness of the agar medium uniform, and the curvature of the curved surface is not particularly limited. Preferably, it is located about 0.2 to 2 mm below the periphery of the opening.

[0010] On the bottom surface of the container-like body of the microorganism testing tool,
The mesh may be printed or molded so as to be a guide when counting the number of microorganisms after the culture.

[0011] The container-like main body and the lid of the microorganism testing tool are
It is not always necessary to use the same material, but it is preferable to use the same material in terms of production efficiency. Further, it is preferable to provide a vertical ridge or groove on the inner peripheral wall of the lid so that a gap for taking in air necessary for the growth of microorganisms can be secured between the lid and the container.

The agar medium to be filled in the container-shaped main body of the microbial testing tool is not particularly limited, and includes, for example, a standard agar medium, a Sabouraud agar medium, a desoxycholate agar medium,
Semi-solid media such as TCBS agar medium and TGSE agar medium are exemplified.

The flexible perforated sheet enclosed in the agar medium of the present invention is not particularly limited, and has a diameter of 0.01 to 0.5 mm.
It may be a net-like material obtained by weaving or welding the linear body at intervals of 3 to 10 mm, a sheet having a through hole with a needle or the like, or a non-woven fabric. The diameter of the through hole is 1 mm or more. However, it is preferable because the agar medium above and below the perforated sheet are integrated. The position of the perforated sheet in the agar medium is preferably 1/4 to 3/4 of the thickness of the agar medium.

The material of the flexible perforated sheet is not particularly limited, and examples thereof include synthetic resins such as polyethylene, polyvinyl chloride, and polyethylene terephthalate.

In order to enclose the flexible perforated sheet in the agar medium, the container-shaped main body is filled with approximately half of the solution agar medium, and after cooling and solidifying, the perforated sheet is placed on the container. Even if the remaining amount of the agar medium is filled, the remaining amount of the agar medium is filled, and the perforated sheet is settled from above the agar medium before the divided and filled agar medium solidifies, Either may be used.

A projection may be provided on the inner peripheral wall of the container-shaped body so that the perforated sheet can be fixed at a fixed position in the agar medium without dividing and filling the agar medium. Even if the projection is formed integrally with the main body, even if another part is fixed with an adhesive or the like,
Either may be used. Also, the position, number, and size of the projections are not particularly limited. For example, they may be provided continuously over the entire circumference or may be provided intermittently.

Also in this case, even after the perforated sheet is held on the projections and then filled with the agar medium, the perforated sheet is settled from above the agar medium before solidifying after filling the agar medium. Or any of them.

The microbial testing device is sterilized by a method such as ethylene oxide gas (EOG) sterilization, radiation sterilization, or electron beam sterilization before filling the agar medium, with EOG sterilization being preferred.

[0019]

According to the present invention, since the container-like body is made of a flexible synthetic resin, the folded edge is pinched and bent by a finger, and the surface of the agar medium filled in the recess is used as a curved inspection material. It can be pressed against the surface, and the flexible perforated sheet is sealed in the agar medium filled in the container-shaped body, so that the surface of the agar medium hardly cracks even when bent, Medium is difficult to peel from container.

[0020]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing one embodiment of the microorganism testing tool of the present invention, FIG. 2 is a cross-sectional view of the container-like main body, and FIG. 3 is a side view of the container-like main body held by fingers. Example 1 In FIG. 1, reference numeral 1 denotes a container-like body of a microbial testing tool formed by vacuum-forming a polyethylene terephthalate sheet.
A folded edge 12 having a circular upper portion and an octagonal lower portion extends outward.

On the inner peripheral wall 14 of the container-like main body 1, projections 15 of approximately 2 mm in height which are approximately 4 mm below the opening 11 and are made of adhesive material are provided at approximately equal intervals so as to project from the medium. A mesh 17 is printed on a convex bottom surface 16 so as to be uniform.

Numeral 2 is a lid formed by vacuum-forming a polyethylene terephthalate sheet. A side wall 22 vertically extending from the periphery of the disk 21 has four inwardly projecting vertical grooves 23 protruding inward. Are provided at substantially equal intervals.

In a clean bench, an agar medium 3 sterilized by high-pressure steam at 121 ° C. for 20 minutes and allowed to cool to about 60 ° C. in a container body 1 previously subjected to an EOG sterilization treatment is about 2 mm from the periphery of the opening 11. It was filled so as to rise. Then the diameter
Diameter of 0.2mm polyethylene filament woven at 3mm intervals
A 30 mm flexible perforated sheet 4 is submerged in the agar medium 3,
While being held on the projections 15, it was further cooled and solidified, the perforated sheet 4 was sealed in the agar medium 3, and the lid 2 was attached.

In use, the lid 2 is removed from the microbial testing tool, and as shown in FIG. 3, the folded edge 12 of the container-shaped main body 1 is bent with fingers and inspected on the surface of the agar medium 3. The material is pressed against the food surface. After reattaching the lid 2,
Incubate at 37 ° C for 24 hours in an incubator. When an incubator is not used, culture at room temperature for 36 to 48 hours. After culture
The number of colonies formed on the surface is calculated, and the test material is determined according to the method for identifying the agar medium used.

In order to demonstrate the difficulty of peeling the agar medium 3 from the container body 1 and the difficulty of cracking of the surface in the first embodiment, the container body 1 filled with the agar medium 3 as described above was used.
Of the agar medium 3 when the peeling of the agar medium 3 from the container-like body 1 and the cracking of the surface were started, the length L of the upper circular portion of the folded edge 12 was measured. Even when the length L of the upper circular portion was changed from the length before deformation of 40 mm to 25 mm, neither peeling nor cracking occurred.

Example 2 A microbial testing tool was obtained in the same manner as in Example 1 except that a container-shaped main body having no projection on the inner peripheral wall was used, and the measurement was performed in the same manner. Length L of upper circular part is 40mm to 25mm
At the time of the deformation, peeling occurred, but no cracking occurred.

Comparative Example 1 Except that the perforated sheet was not enclosed in the agar medium,
A device for testing microorganisms was obtained in the same manner as in Example 1, and the measurement was performed in the same manner. When the length L of the upper circular portion was changed from 40 mm to 32 mm, cracking of the surface of the agar medium started, and when the length L was changed to 30 mm, peeling started.

Comparative Example 2 A microbial testing tool was obtained in the same manner as in Example 1 except that a perforated sheet was not sealed in the agar medium, except that a container-shaped main body having no projection on the inner peripheral wall was used. Was measured. At the time when the length L of the upper circular part was changed from 40 mm to 36 mm, the agar medium was completely peeled off from the container-like body,
No cracking occurred.

[0029]

[Effects of the Invention] Since the present invention is configured as described above, the surface of the agar medium is hardly cracked and the agar medium is hardly peeled from the container.

[Brief description of the drawings]

FIG. 1 is a perspective view showing an example of a microorganism testing tool of the present invention.

FIG. 2 is a sectional view of the container-shaped main body of FIG.

FIG. 3 is a side view of the container-like main body in a state where the main body is held by fingers.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Container-shaped main body 2 Lid 3 Agar medium 4 Perforated sheet 11 Opening 12 Folded edge

Continuation of the front page (56) References JP-A-63-214176 (JP, A) JP-A-47-11988 (JP, A) JP-A-6-57199 (JP, U) JP-A-62-130499 (JP, A) , U) Japanese Utility Model Showa 60-179199 (JP, U) Japanese Utility Model Showa 60-62300 (JP, U) Japanese Utility Model Showa 54-88097 (JP, U) (58) Fields surveyed (Int. Cl. ⁶ , DB Name) C12M 1/00-3/10

Claims (1)

(57) [Scope of request for utility model registration] 1. An agar medium filled with a container-shaped main body, comprising a container-shaped main body made of a flexible synthetic resin and a lid, and having a folded edge extending outward at the periphery of an opening of the container-shaped main body. A microbial testing tool, wherein a flexible perforated sheet is enclosed.