JP2548216Y2 - Culture vessel - Google Patents

Description

[Detailed description of the invention]

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture vessel used for plant tissue culture.

[0002]

2. Description of the Related Art Conventionally, as shown in FIG. 4, a generally cylindrical general-purpose molded bottle composed of a main body 31 and a lid 32 is used as a culture container for tissue culture of plants. This culture vessel is manufactured by injection molding a hard and transparent plastic such as a polycarbonate resin.

A male screw 33 is formed in the opening of the main body 31 of the culture vessel, while a male screw 33 is formed inside the lid 32.
Is formed, and the culture vessel is completely sealed by screwing the lid 32 to the main body 31. The diameter of the opening of the main body 31 where the male screw 33 is formed is smaller than the diameter of the main body 31 itself.

[0004] When a plant to be tissue-cultured (hereinafter referred to as a plant) is to be planted in the culture vessel, first, an agar medium containing nutrients necessary for the growth of the plant is prepared inside the main body 31. After dissolving and dispensing under heat, dispensing by heating, for example, in an autoclave, planting a plant body, and then screwing the lid 32 and culturing the cells in a culture shelf, culture facility or culture facility or the like set to predetermined conditions. .

[0005]

However, the above-mentioned conventional culture vessel has a circular bottom surface, so that, for example, when a large number of culture vessels are accommodated in one culture facility, they cannot be arranged without gaps, and the culture is not performed. Dead space is created inside the equipment. In addition, when removing a plant cultured inside the culture container, for example, by transplanting work performed for the purpose of increasing the number of plants, it takes time to open and close because the lid is screwed,
In addition, since the bottom of the main body is deep, it is very difficult to remove the main body, so that the workability is poor, the risk of contamination by bacteria increases, and there is a possibility that the plant body may be damaged by tweezers or a narrow opening of the main body.

[0006] Further, since the culture vessel is completely closed, the inside becomes excessively moist, and furthermore, sufficient oxygen is not supplied, the plant grows fragile, and water drops adhere to the inside of the main body. It becomes cloudy and the growth of the plant is difficult to observe from the outside.

[0007] Further, since this culture vessel cannot be overlapped with the main body and the main body, and the lid with the lid, it is not possible to treat a large amount at a time when heating and sterilizing in an autoclave.
When storing, a bulky storage space is required, and when the culture vessel is washed after culturing the plant, the inside of the body is difficult to wash because the bottom of the body is deep.

[0008]

In order to solve the above-mentioned problems, a culture vessel of the present invention is a culture vessel used for plant tissue culture, and has a main body and a lid. The bottom surface and the opening surface are substantially square, the opening surface is larger than the bottom surface, the height of the main body is shorter than the length of one side of the opening surface of the main body, and the main body and the lid are locked. The main body and the lid have respective locking surfaces,
A locking means capable of adjusting a gap with the lid is provided, and a gap is always formed between the main body and the lid by the locking means.

[0009] Further, in a culture vessel used for tissue culture of a plant, a point-like projection engaging with a lattice-like projection provided outside the bottom surface of the main body portion is provided outside the top surface of the lid portion. It is characterized by.

[0010] Further, the present invention is a culture vessel used for plant tissue culture, wherein the main body and the main body, the lid and the lid can be overlapped with each other, and the amount of culture medium is placed outside the side surface of the main body. It is characterized in that a scale for measuring is formed.

[0011]

According to the above construction, since the bottom of the main body is substantially square, for example, when a large number of culture vessels are accommodated in one culture facility, they are arranged almost without gaps, and a dead space is generated inside the culture facility. Absent. In addition, when removing a plant cultured inside the culture container by, for example, a transplanting operation performed for the purpose of increasing the number of plants, the lid is locked by simply pressing lightly on the main body, so that the lid can be detached. In addition, since the bottom surface of the main body portion is smaller than the opening surface, it is very easy to take out and the workability is improved, and there is no danger of damaging the plant with tweezers.

In addition, a gap is always formed between the main body and the lid, so that the inside of the culture vessel is not completely sealed, and a proper air permeability and air tightness are maintained. Sufficient oxygen is supplied to promote the growth of the plant, and water droplets hardly adhere to the inside of the main body, so that it does not fog and the growth of the plant can be easily observed from the outside.
To prevent unnecessary evaporation of water during
It can keep out dust and the like.

Further, the culture vessel can be placed in a small storage space without being bulky when storing because the main body and the main body or the lid and the lid can be overlapped, and the culture vessel is washed after culturing the plant. When doing so, the inside of the main body is easy to wash because the depth of the main body is shorter than the length of one side of the opening surface.

In addition, since a scale indicating the amount of the agar medium is formed outside the side surface of the main body, the work of measuring the agar medium in another container can be omitted, and the grid provided outside the bottom surface of the main body can be omitted. Since the point-like projections engaging with the projections are provided outside the top surface of the lid, the culture vessels do not easily slide down when the culture vessels are stacked.

[0015]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be described below with reference to FIGS.

The culture vessel according to the present invention comprises a main body 1 and a lid 11 as shown in FIG. As shown in FIG. 2, the main body 1 is made by injection-molding a heat-resistant hard / transparent plastic such as a polycarbonate resin. Since the bottom surface 2 and the opening surface 3 of the main body 1 are substantially square and the opening surface 3 is wider than the bottom surface 2, the side surfaces 4 of the main body 1 have a substantially inverted trapezoidal shape and head toward the opening surface 3. It is inclined so that it spreads out according to. In addition, since the height 4a of the main body side surface 4 is set to be smaller than the length 3a of one side of the opening surface 3, the main body 1 is formed in a container having a shallow bottom with respect to the size of the opening surface 3. Also, the bottom sides 5a,... Of the inner surface 5 of the main body 1 are subjected to curved surface processing, and various scales are provided outside the main body 1 as described later.
The inner surface 5 is completely smooth.

On the outer side near the opening surface 3 of the main body 1, a lid 1 is provided.
When the main body 1 is pressed against the main body 1, main body side locking portions 6... Which lock the lid portion 11 are provided perpendicular to the bottom surface 2. Although two locking projections 7a and 7b are provided in parallel with each side of the opening surface 3, the locking projections of the lid side locking portion 16 of the lid 11 by the locking projections 7a and 7b are provided. The method of locking with 17 will be described later.

Further, two of the four side surfaces 4...
First, a medium volume scale 8 is provided so that the amount of agar to be poured into the main body 1 can be seen at a glance when producing an agar medium described later.

After the agar medium is prepared inside the main body 1 on the bottom surface 2, a plurality of plants (hereinafter referred to as "plants") to be cultured on the agar medium can be arranged neatly when planted. A grid-like projection 9 of a grid is provided. The medium volume scale 8 and the grid-like projections 9
All are provided outside the main body 1.

Next, as shown in FIG. 2, the lid 11 is made by injection molding a heat-resistant hard and transparent plastic such as a polycarbonate resin of the same quality as the main body 1. The top surface 12 and the opening surface 13 of the lid portion 11 are substantially square, and the opening surface 13 is wider than the top surface 12, so that the side surfaces 14 of the lid portion 11 are substantially trapezoidal. It is inclined to spread outward as it goes. Also, the bottom sides 15a... Of the inner surface 15 of the lid 11 are subjected to a curved surface treatment, and the inner surface 15 is completely smooth.

On the outer side near the opening surface 13 of the lid portion 11, lid side locking portions 16 for locking the lid portion 11 with the main body portion 1 when the lid portion 11 is pressed against the main body portion 1 are provided on the top surface. The lid-side locking portions 16 are provided with locking projections 17 parallel to each side of the opening surface 13. A method of locking the main body side locking portion 6 of the main body 1 with the locking projections 7a and 7b will be described later.

The size of the top surface 12 of the lid 11 is the same as the size of the bottom surface 2 of the main body 1, and the size of the opening surface 13 of the lid 11 is the size of the opening surface of the main body 1. 3 is substantially equal to the sum of the thickness of the main body 1 and the height of the locking projection 17 in addition to the size of the main body 3. Therefore, when the lid 11 is locked to the main body 1, a gap substantially equal to the height of the locking projection 17 is formed between the lid side locking portion 16 and the main body side locking portion 6. It has become.

On one of the four side surfaces 14 of the lid 11, there is provided a sticking part 1 on which a label describing the kind of the plant planted on the agar medium inside the main body part 1, the culture conditions, and the like is written.
8 are provided, and the sticking portion 18 has fine irregularities on the surface, so that the label can be easily peeled off.

Outside the top surface 12, a grid of squares provided outside the bottom surface 2 of the main body 1 to prevent the culture containers from easily sliding down due to vibration or the like when the culture containers are stacked. Point-like projections 19 for engaging with the projections 9 are provided. When the culture vessels are stacked, the point-like projections 19 ...
... arranged so as to match the part.

Next, the locking projections 7a and 7b provided on the main body side locking portion 6 of the main body 1 and the lid side locking portion 16 of the lid portion 11 are provided.
The locking of the locking projections 17 provided in the first embodiment will be described below with reference to FIGS.

First, the locking projections 7a and 7b provided on the main body side locking portion 6 of the main body portion 1 and the lid side locking portion 16 of the lid portion 11 are provided.
The locking projection 17 provided on the main body 1 is locked by lightly pressing the lid 11 against the main body 1 from above. At this time, the main body side locking portion 6 of the main body portion 1 and the lid portion 11
Are slightly distorted, and the locking projections 17 provided on the lid-side locking portion 16 of the lid portion 11 are engaged with the engaging portions provided on the main-body-side locking portion 6 of the main body 1. It is pushed down while abutting on the stopping projection 7a, and exceeds the locking projection 7a.
It stops by hitting the locking projection 7b.

That is, the lid 11 is locked over the main body 1. At this time, a gap of several mm through which air can sufficiently flow is formed between the edge 1a of the opening surface 3 of the main body 1 and the step 11a of the opening surface 13 of the lid 11. ing.

When the lid 11 is further pressed against the main body 1, the locking projections 17 provided on the lid side locking portion 16 of the lid 11 are moved to the main body side locking portion 6 of the main body 1. The lid 11 is pressed down while being in contact with the provided locking projection 7b, and goes over the locking projection 7b so as to cover the main body 1 more deeply than before and is locked.

In this case, the edge 1a of the opening 3 of the main body 1 and the step 11a of the opening 13 of the lid 11 come into contact with each other so that the main body 1 does not fit into the lid 11 more than necessary. ,
A small gap is formed between the main body 1 and the lid 11 so that air can flow appropriately.

When removing the lid 11 from the main body 1,
By pressing the side surface 4 of the main body 1 and pulling the lid 11 upward, the main body side locking portion 6 of the main body 1 and the lid 1
1 are slightly distorted, and the main body 1
The locking projections 7a and 7b provided on the main body locking portion 6 and the locking projection 17 provided on the lid locking portion 16 of the lid 11.
Is released, and the lid 11 can be removed from the main body 1.

An example of a method for culturing a plant using the culture vessel having the above-described structure will be described below.

First, a culture vessel and LS (Lynsmeyer a
nd Skoog) An agar medium containing nutrients and plant hormones necessary for the growth of a plant such as an agar medium is heat-sterilized in an autoclave, for example. At this time, the culture container is
Since the lids 11 and the lids 11 can be overlapped and stacked in a heat-resistant autoclave bag or the like, they can be efficiently sterilized by heating with an autoclave.

Next, after dispensing a predetermined amount of the agar medium into the main body 1 in a sterile environment such as a clean bench, the lid 11 is lightly pressed against the main body 1 from above, and the main body of the main body 1 is pressed. Locking projections 7a and 7b provided on side locking portion 6 and lid 1
The locking projection 17 provided on the lid side locking portion 16 of the first embodiment is locked to lock the main body 1 and the lid 11 so that dust and the like do not enter the agar medium.

At this time, the culture vessel is provided with a medium volume scale 8, and a separate vessel is not required for measuring the agar medium, so that the agar medium can be easily dispensed. When the locking projections 7a and 7b provided on the stopping portion 6 and the locking projection 17 provided on the lid-side locking portion 16 are locked, a few mm is provided between the main body 1 and the lid 11. Since the gap is open, air circulation is good and the evaporation of water in the agar medium and the coagulation of the agar medium are promoted, and water droplets do not adhere to the inside of the culture vessel.

Next, the cover 11 is removed from the main body 1 and a plant such as tobacco is planted on the agar medium. At this time, since the bottom of the main body 1 is shallow, the work is easy to perform, and since the lattice-like projections 9 are provided outside the bottom surface 2 of the main body 1, the lattice-like projections 9 are used as a guide for one culture vessel. Multiple plants can be planted in an orderly manner.

Thereafter, the lid 11 is pressed against the main body 1 again so that the edge 1a of the opening 3 of the main body 1 and the step 11a of the opening 13 of the lid 11 come into contact with each other. The main body 1 is inserted deeply into the lid 11 to prevent unnecessary evaporation of water in the agar medium and prevent dust and the like from entering the agar medium. At this time, the culture vessel is not completely sealed, and a slight gap is left between the main body 1 and the lid 11, so that the culture vessel is maintained in a suitable airtightness and air permeability, and is kept in a plant body. Sufficient oxygen is supplied to promote plant growth. Further, since water droplets evaporating from the agar medium hardly adhere to the inside of the culture container, the water does not fog and the growth state of the plant is easily observed from the outside.

Then, a large number of the plants thus treated are put into a culture facility or the like set under predetermined conditions and cultured. In this case, since the bottom surface 2 of the main body 1 is substantially square, the culture vessels are arranged in the culture equipment with almost no gap, and no dead space occurs in the culture equipment. In addition, the point-like projections 19 provided outside the top surface 12 of the lid portion 11 and the lattice-like projections 9 provided outside the bottom surface 2 of the main body portion 1 engage with each other.
When the culture vessels are stacked, the upper culture vessel does not slide down easily. Then, even if the plant grows and becomes tall, it is only necessary to replace the lid 11 with a tall one according to the growth of the plant, and it is not necessary to replace the plant with another culture vessel.

After culturing for a certain period of time using a cultivation facility or the like, the plants in the culture vessel are taken out with tweezers or the like, and are subcultured, for example, to increase the number of plants. Also at this time, since the bottom of the main body 1 is shallow, work can be easily performed, and the plant can be easily taken out from the main body 1 without damaging the plant.
Alternatively, the division of the buds can be performed without removing the plant from the main body 1.

When the culture vessel is washed after the cultivation of the plant is completed, the inner surface 5 of the main body 1 has no dent or the like, so that the agar medium can be easily removed. Since the main bodies 1 and the lids 11 can be overlapped with each other, they can be efficiently immersed in the detergent liquid. At this time, if the label has normal adhesiveness, the label easily peels off from the sticking portion 18 due to the minute unevenness of the sticking portion 18 of the lid portion 11.

Since the culture vessel has little irregularities in the main body 1 and the lid 11, and the bottom of the main body 1 is shallow, it is possible to sufficiently remove dirt even when automatic washing is performed by an automatic washing machine. it can.

As described above, since the bottom of the main body is substantially square, the culture vessel having the above-described configuration is arranged, for example, with almost no gap inside the culture equipment, and the lid is engaged only by lightly pressing the main body. Since the lid is stopped, the lid can be easily attached and detached, and since the bottom surface of the main body is smaller than the opening surface, it is very easy to take out and the workability is improved, and there is no danger of damaging the plant. In addition, since a scale indicating the amount of the agar medium is formed outside the side surface of the main body, it is not necessary to weigh the agar medium in a separate container, and it is connected to the grid-like projections provided outside the bottom surface of the main body. Since the matching point-like projections are provided outside the top surface of the lid, the culture vessels do not easily slide down when the culture vessels are stacked.

Further, not inside the complete sealed state of the culture vessel, since adequate ventilation and the tightness is maintained, sufficient oxygen is supplied to the plant and promotes growth of plants
In both cases, extra evaporation of water occurs during plant cultivation.
It is possible to prevent dust and the like from entering the agar medium . Furthermore, since the main body and the main body or the lid and the lid can be overlapped, they are not bulky when storing them, so that only a small storage space is required, and the structure is very easy to wash when washing.

[0043]

As described above, the culture vessel of the present invention has a main body and a lid, the bottom surface and the opening surface of the main body portion are substantially square, and the opening surface is larger than the bottom surface. The height of the main body portion is shorter than the length of one side of the opening surface of the main body portion, and the main body portion and the lid portion each have a main body portion and a lid portion each of which has a main body portion. And the gap between the lid
Adjustable locking means is provided, and a gap is always formed between the main body and the lid by the locking means.

Further, a point-like projection which engages with a lattice-like projection provided outside the bottom surface of the main body is provided outside the top surface of the lid.

Further, the main body and the main body, the lid and the lid can be overlapped, and a scale for measuring the amount of culture medium is formed outside the side surface of the main body.

Therefore, since the bottom of the main body is substantially square, for example, when a large number of culture vessels are accommodated in one culture facility, they are arranged almost without gaps, and no dead space is generated inside the culture facility. In addition, when removing the plant body cultured in the culture vessel by, for example, transplanting work, the lid is locked only by lightly pressing the main body, so that the lid can be easily attached and detached. Since the bottom surface is smaller than the opening surface, it is very easy to take out and the workability is improved, and there is no fear of damaging the plant with tweezers.

In addition, a gap is always formed between the main body and the lid, so that the inside of the culture vessel is not completely sealed, and a proper air permeability and air tightness are maintained. Sufficient oxygen is supplied to promote the growth of the plant, and water droplets hardly adhere to the inside of the main body, so that it does not fog and the growth of the plant can be easily observed from the outside.
To prevent unnecessary evaporation of water during
It can keep out dust and the like.

Further, the culture container can be placed in a small storage space without being bulky when sterilized by an autoclave or the like or when stored for storage because the main body and the lid or the lid can be overlapped. In addition, a small water tank is sufficient when the plant is immersed in a detergent solution to wash the culture vessel after the culture of the plant. Also, when cleaning
Since the depth of the main body is shorter than the length of one side of the opening surface and there is no dent or the like on the inner surface, the inside of the main body can be easily washed.

In addition, since the scale indicating the amount of the agar medium is formed outside the side surface of the main body, it is not necessary to measure the agar medium in a separate container, and the grid provided outside the bottom of the main body is also provided. Since the point-like projections that engage with the projections are provided outside the top surface of the lid, an effect is provided that the culture vessels do not easily slide down when the culture vessels are stacked.

[Brief description of the drawings]

FIG. 1 is a schematic view of a culture vessel according to an embodiment of the present invention.

FIG. 2 is a perspective view of a main body and a lid of the culture vessel.

FIG. 3 is a schematic cross-sectional view showing a locked state of a main body and a lid of the culture container.

FIG. 4 is a sketch of a conventional culture vessel.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Main body part 2 Bottom surface 3 Opening surface 4 Side surface 6 Main body side locking part 7a Locking protrusion 8 Medium scale 9 Grid-shaped protrusion 11 Lid part 12 Top surface 13 Opening surface 16 Lid part locking part 17 Locking protrusion 19 dot projection

Claims (3)

(57) [Scope of request for utility model registration] 1. A culture vessel for use in plant tissue culture, comprising a main body and a lid, wherein the bottom surface and the opening surface of the main body portion are substantially square, and the opening surface is larger than the bottom surface. The height of the main body portion is shorter than the length of one side of the opening surface of the main body portion, and the main body portion and the lid portion each have a main body portion and a lid portion each of which has a main body portion. And the lid
A culture vessel provided with locking means capable of adjusting a gap, wherein the locking means always forms a gap between the main body and the lid. 2. The culture vessel according to claim 1, wherein point-like projections that engage with lattice-like projections provided outside the bottom surface of the main body are provided outside the top surface of the lid. 3. The apparatus according to claim 1, wherein the main body and the main body, the lid and the lid are overlapped with each other, and a scale for measuring the amount of the culture medium is formed outside the side surface of the main body. The culture container according to claim 1 or 2, wherein