JPH0539300U - Plant culture vessel - Google Patents

Abstract

(57) [Summary] [Structure] The lid 2 is provided with a vent hole 5, and the vent hole is provided with a heat resistant filter 4. This lid 2 and container body 1
~ Screw with a thread having 6 threads. [Effect] The sterilization can be reliably performed, and the sterilized state can be maintained for a long time.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to a culture container used for growing and growing a plant.

[0002]

[Prior Art]

 Conventionally, sterilization has been regarded as an important technique for eliminating tissue of other organisms, especially microorganisms, from the viewpoint of pure culture regardless of microorganisms, animals or plants in tissue culture. Among them, the culture vessel and medium directly maintain and grow the target organism purely, and thus complete sterilization and long-term maintenance of the sterilized state often greatly affect the progress of research. Until now, sterilization methods such as dry heat sterilization, high pressure steam sterilization, gas sterilization, and γ-ray sterilization have been developed.However, in the case of plant culture, the medium used contains agar and High-pressure steam sterilization has been widely used since before because of the fact that relatively few decompose with high heat.

On the other hand, as a culture container for plants, a glass transparent container with an aluminum foil stopper, a cotton stopper, or a plastic or aluminum cover has been used for a long time. .. These containers had the advantage of not only maintaining proper aeration during culturing, but also very high sterilization efficiency during high-pressure steam sterilization. However, these containers have a problem in terms of hermeticity and have a drawback that contamination of various bacteria is more likely to occur as the culture period becomes longer and the opening diameter becomes larger. It was insufficient in terms of maintenance.

Therefore, in order to solve this problem, a lid was devised in which a small hole was formed in the center of the rubber stopper and a cotton stopper was filled in the small hole. As a result, the tightness between the container and the lid was improved, and since aeration was performed through a cotton plug, it was less likely to be contaminated with various bacteria even after long-term culture. However, this lid is cumbersome to use because it is filled with cotton plugs one by one, and in addition to the drawback that the amount of air flow depends on how the cotton is filled, in some cases a great deal of force is required to attach and detach the container and lid. It had a problem that it was necessary.

By the way, in order to improve the culture and growth conditions of microorganisms, animals, and plants, some screw-type lids having a ventilating hole to which a hydrophobic filter is attached are provided so as to aseptically supply air. It has been developed (Japanese Patent Laid-Open No. 1-503595, Japanese Patent Laid-Open No. 2-23079). However, when a plant culture vessel is created using this invention, very high results can be obtained in terms of plant growth and maintenance of the sterilization state. Since high-pressure steam passes through the filter, it cannot withstand heat depending on the material of the filter, and depending on the molding method of the filter, pore closure may occur (especially the filter with a single-layer porous membrane), resulting in insufficient sterilization. However, there is a drawback that the filter may be peeled off, or may be cracked or deformed in extreme cases depending on the material of the container body.

[0006] Furthermore, the lid is usually detached from the container body with one hand due to the operational characteristics of the aseptic operation performed in plant culture. Therefore, it is highly necessary that the lid is detached by the rotation of one rotation or less. However, when the lid which can be attached and detached by the ordinary screw structure in one rotation or less is prepared by using the above-described invention, high-pressure steam sterilization There is a problem that the lid comes off due to the difference in internal pressure and external pressure at the time, and the above-mentioned invention is insufficient in application to a plant culture vessel.

[0007]

[Problems to be solved by the device]

 The object of the present invention is to solve the above-mentioned problems. Specifically, during sterilization with steam at high pressure and high temperature, the inside of the container and the medium are completely sterilized, and the sterilized state is maintained for a long period of time. The purpose of the present invention is to provide a plant culture container that can be maintained for a long time.

[0008]

[Means for Solving the Problems]

 The present invention is a container for cultivating a plant, wherein a heat-resistant resin packing is provided on the inner wall surface of the lid facing the opening end of the container body, and / or the opening end of the container body. The screw-type lid, which has a loop-back structure of the same diameter as the inner diameter of the opening of the container body, is attached to the lid and the inner wall surface of the opening so that gas can pass therethrough A ventilation hole with a filter laminated with a high-density laminated fiber that is impenetrable to heat and has a heat resistance of at least 120 ° C is installed, and the container body and the lid body have 2-6 thread threads. The present invention provides a plant culture container characterized by being connected by a screw structure using.

Hereinafter, an embodiment of the present invention will be described. FIG. 1 is a partially enlarged sectional view of a container according to the present invention, and FIG. 2 is a partially enlarged sectional view of a container according to the present invention. Reference numeral 1 is a container body, and 2 is a lid. The container body 1 can be basically made of any material as long as it has resistance to heat of 120 ° C., but glass, polycarbonate, and polymethylpentene are used from the viewpoint of light transmittance. Preferably.

The lid body 2 can be made of any material as long as it is basically resistant to heat of 120 ° C. like the container body 1, but from the aspect of fitting with the container body 1. Preference is given to using polypropylene, polyallomers, polyethylene.

The lid body 2 is provided with an annular folded structure 3 as shown in FIG. 1 and can be hermetically sealed on two surfaces of a container opening inner wall surface 6 and a container opening end portion 7, or as shown in FIG. It is preferable to have any one of the sealed structures having the annular packing 10 made of a soft resin that can be adhered to the.

Further, the lid 2 is provided with a vent hole 5, gas can pass through the upper surface or the lower surface of the vent hole, impermeable for microorganisms, and heat resistance of at least 120 ° C. A filter 4 in which fibers are laminated at a high density is attached. If the heat resistance of the fiber is less than 120 ° C, high-pressure steam sterilization cannot be performed.

Any material can be used for the filter 4 as long as it has heat resistance to 120 ° C. However, from the viewpoint of heat resistance, glass, ceramic, cellulose, polyamide, polyimide, polypropylene, polyethylene, polyester , Fluorocarbon resin, and / or protein fiber are preferable, and glass, ceramic, cellulose, polyamide, and / or polypropylene fiber is preferable in terms of permeability to high-pressure steam.

The ventilation hole 5 to which the filter 4 is attached allows high-pressure steam to smoothly pass therethrough to completely sterilize the inside of the container, and further, due to the closed structure such as the annular folded structure 3 and the annular packing 10 described above, Sterilization is maintained for a long time. Although the principle that a high-density laminated fiber type filter allows high-pressure vapor to pass through more smoothly than a single-layer porous membrane type filter is not always clear, a fiber laminated filter has the same effect as a cotton plug. It is considered to have.

The container body 1 and the lid body 2 are connected by an outer screw of the container body 1 and an inner screw of the lid body 2. These screw structures preferably have a thread having 2 to 6 threads. .. If a structure with a single thread is attached and detached in less than one rotation, the connection between the main body and the lid will be insufficient. On the other hand, those having 7 or more threads are not preferable because the structure becomes complicated. Due to the multi-threaded structure, the container body 1 and the lid body 2 can be firmly connected to each other even if the attachment / detachment rotation is one rotation or less, so that the lid body can be prevented from coming off due to the difference in internal and external pressures during high-pressure steam sterilization. ..

FIG. 3 shows the bottom surface 11 of the container body 1. As a guide for recognizing the position of an arbitrary region and / or point on the bottom surface 11, a grid pattern such as 12 is formed on the bottom surface. Providing protrusions, grooves, or printing a grid pattern is preferable in terms of uniform implantation operation.

The shape of the container body may be any of a cylindrical shape, a cubic shape, a rectangular parallelepiped shape, a pyramidal shape, a conical shape, a truncated cone shape, a truncated pyramid shape, and an upper conical lower rectangular parallelepiped shape. From the viewpoint of the possible volume and the ease of attaching / detaching the lid, the upper conical lower rectangular shape is preferable.

[0018]

【Example】

 An embodiment of the present invention will be shown below. A lid having a closed structure with an annular folded structure as shown in FIG. 1 was provided with a ventilation hole with a diameter of 1 cm, and glass, cellulose and polypropylene fiber filters were attached as high density laminated fiber filters there. Then, two flasks each tightly closed with the lid attached were prepared. Then, this was subjected to high-pressure steam sterilization at 121 ° C. for 20 minutes, and the state was examined. The confirmation of the sterilization state inside the flask was performed by inserting an autoclave indicator cut into 17.5 cm vertically and judging by the length of the sterilized region. As a result, the bottom of the flask was sterilized.

[0019]

[Effect of the device]

 Since the present invention has the above configuration, it has the following unique effects. It is possible to completely sterilize the inside of the container during high-pressure steam sterilization, and the sterilized state is maintained for a long period unless the lid is removed.

In addition, when used in the acclimation process performed at the time of transplantation from a culture state to a field, the acclimation efficiency is improved due to the high water vapor permeability peculiar to the fiber high-density laminated filter. The multi-threaded screw allows the lid to be attached and detached in less than one rotation, while maintaining a firm connection.

[Brief description of drawings]

FIG. 1 is an enlarged sectional view of a part of a container according to the present invention.

FIG. 2 is an enlarged sectional view of a part of another container according to the present invention.

FIG. 3 is a bottom view of the container 1 of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Container body 2 Lid body 3 Annular folding structure 4 Organic fiber high density laminated filter 5 Vent hole 6 Container opening inner wall surface 7 Container opening end 8 Container body outer screw thread 9 Lid body screw thread 10 Annular packing 11 Container body bottom surface 12 Grid guide

Claims (8)

[Scope of utility model registration request] 1. A container for culturing a plant, wherein a heat-resistant resin packing is provided on an inner wall surface of a lid body facing an opening end of the container body, and / or an opening end of the container body. Portion and the inner wall surface of the opening are closely attached to each other, a screw-type lid body having an annular folded structure having the same diameter as the inner diameter of the opening portion of the container main body allows gas to pass therethrough and allows passage of microorganisms. Is not possible, and a ventilation hole provided with a filter in which fibers having a heat resistance of at least 120 ° C. are densely laminated is provided, and the container body and the lid are 2 to 6
A plant culture container, which is connected by a screw structure using a thread of a strip. 2. The plant culture container according to claim 1, wherein the material of the container body and the cover is glass, polypropylene, polycarbonate, polyethylene, polymethylpentene, polyallomer, polysulfone, or fluorocarbon resin. .. 3. The plant culture according to claim 1, wherein the material of the filter attached to the ventilation hole is glass, ceramic, cellulose, polyamide, polyimide, polypropylene, polyethylene, polyester, fluorocarbon resin, and / or protein fiber. container. 4. The container body and lid are made of glass, polypropylene, polycarbonate, polyethylene, polymethylpentene, polyallomer, polysulfone, or fluorocarbon resin, and the material of the filter attached to the vent is Glass, ceramic, cellulose,
The plant culture container according to claim 1, which is one of polyamide, polyimide, polypropylene, polyethylene, polyester, fluorocarbon resin, and protein fiber. 5. The container body is made of glass, polycarbonate and polymethylpentene, the lid is made of polypropylene and polyethylene, and the filter attached to the ventilation hole is made of glass, ceramic, cellulose or polyamide. The plant culture container according to claim 1, which is and / or polypropylene fiber. 6. The container according to claim 1, wherein the shape of the container body is any one of a columnar shape, a cubic shape, a rectangular parallelepiped shape, a pyramidal shape, a conical shape, a truncated cone shape, a truncated pyramid shape, and an upper conical lower rectangular parallelepiped shape. Plant culture vessel. 7. The method according to claim 1, wherein protrusions or grooves are provided in a grid pattern or a grid pattern is printed on the bottom surface of the container body as a guide for recognizing the position of an arbitrary area and / or point on the bottom surface of the container. Plant culture vessel. 8. The material of the container body is glass, polycarbonate, polymethylpentene, the material of the lid is polypropylene, polyethylene, and the material of the filter attached to the ventilation hole is glass, ceramic, cellulose, polyamide, and / or Alternatively, polypropylene fiber is used, and the shape of the container body is a rectangular parallelepiped in the shape of an upper cone. As a guide for recognizing the position of any area and / or point on the bottom surface, protrusions or grooves are formed in a grid pattern on the bottom surface or a grid pattern is printed. The plant culture container according to claim 1.