JPH0350719Y2 - - Google Patents

Description

[Detailed description of the invention] (Field of industrial application) The present invention relates to a container used for tissue culture.

(Prior Art) In tissue culture, culture containers such as test tubes, flasks, culture bottles, and culture tanks are used to artificially grow a portion of tissue or organs that have been separated from the mother's body. Glass is common, but there are also synthetic resin and metal ones. Aluminum foil, a synthetic resin overcap or cotton can be used as a stopper. Aluminum foil and overcaps can prevent the medium from drying out better than cotton plugs, but their drawback is that they have poor ventilation.

The plastic container proposed in Utility Model Application No. 61-118300 was an attempt to improve these drawbacks, and consists of a container body and a lid, with a convex part provided on one of the fitting surfaces to improve air permeability. It's what I gave.

(Problems to be solved by the invention) Although tissue culture requires sterile culture, breathability is required for the following reasons. After dispensing the culture medium into a sterilized culture container and autoclaving it with the lid on,
Let stand at room temperature until the agar in the medium hardens. Tissues are implanted into the culture medium in the culture container prepared in this way, but at this time the inside of the culture container is so humid that water droplets adhere to the inner wall due to the water vapor released from the culture medium during autoclaving. . Therefore, in order to culture the implanted tissue, it is left in a superhumidified state 25
When placed in a constant temperature chamber at ~27°C, the overhumidity becomes increasingly excessive, resulting in an adverse effect on tissue growth. In addition, in the case of plant tissue, the oxygen and carbon dioxide gas in the container are consumed quickly, and it is easy to fall into a state of deficiency.

The present invention seeks to provide a new container to replace conventional culture containers that have no or insufficient ventilation.

(Means for Solving the Problems) The present invention is an improved culture container consisting of a combination of a container body made of transparent synthetic resin and a lid. A porous separation membrane with a pore diameter of 0.1 to 0.5 μm formed to a size that can cover the through holes and partially has an adhesive layer peeled from the release paper on its back surface, and the adhesive layer is attached to the lid. It is characterized in that it is adhered from the outer surface of the body to the periphery of the through hole to close the through hole.

(Function) In the present invention, by appropriately adjusting the type of separation membrane, the volume of the container, and the size of the through holes, it is possible to adjust and maintain an optimal ventilation state.

Therefore, when a culture medium is dispensed into the culture container according to the present invention and placed in an autoclave, the water vapor released from the culture medium can pass through the separation membrane and escape to the outside of the container. Similarly, even when the tissue is implanted and placed in a constant temperature chamber, the inside of the container does not become overhumidified because the separation membrane provides ventilation.

In addition, when reusing a container, when cleaning the used container, remove the old separation membrane from the lid.
Instead, a new separation membrane that has been peeled off from the release paper may be attached.

(Example) Hereinafter, an example of the present invention will be described with reference to the drawings.

FIG. 1 shows a sectional view of a culture container according to an example. In the figure, a container body 1 made of transparent synthetic resin is fitted with a lid 2 made of the same material. It is not an absolute condition that the fitting surface between the container body 1 and the lid body 2 be airtight;
Breathability may be provided as in No. 61-118300. Furthermore, the lid body 2 is provided with a through hole 3, and a separation membrane 4 is adhered to the outer surface of the hole 3.

The separation membrane 4 is designed to prevent the infiltration of germs.
On the other hand, the pore size is 0.1 to maintain breathability.
A porous membrane of about ~0.5μ is used. A convenient method for attaching it to the lid body 2 is to use an adhesive that is applied partially to the porous membrane, for example in a donut shape, leaving the center intact. If you prepare in advance a number of separation membranes that are slightly larger than the size of the through hole 3 and pasted on release paper using adhesive, each time you reuse the culture container, you can peel off the old separation membrane and make a new one. A separation membrane can be attached.

(Effects of the invention) According to this invention, it is possible to protect the cultured tissue from bacteria, and the interior does not become overhumidified and has excellent ventilation, so healthy tissue can always be cultured under optimal conditions. can do. Furthermore, since the separation membrane is easy to replace, the container can be used in the same condition as new each time it is reused.

[Brief explanation of the drawing]

FIG. 1 is a sectional view of a culture container according to an embodiment of the present invention. 1...Container body, 2...Lid, 3...Through hole, 4
...Separation membrane.

Claims (1)

[Scope of utility model registration request] In a tissue culture container consisting of a container body made of transparent synthetic resin and a lid made of transparent synthetic resin attached to the container body, a through hole is bored in the lid and the through hole is covered. A porous separation membrane with a pore diameter of 0.1 to 0.5 μm, which is formed to a size that allows the adhesive layer to be peeled off from the release paper and partially on its back surface, is removed from the outer surface of the lid body. A tissue culture container characterized in that the through hole is sealed by being adhered around the through hole.