JPH06122490A - Culture container - Google Patents

Abstract

PURPOSE:To obtain an inexpensive, disposable sterile culture container which is used in the culture of plant tissue, easy to produce, good in operational efficiency in sterilization, sample filling, etc., and suited for the acceleration of photosynthesis or observation. CONSTITUTION:The culture container comprises, in combination, a transparent plastic container 1 capable of being sterilized under pressure and heat and made by sheet forming method with a flange 3 on its opening and a lid 2 made of a laminate material constituted of a transparent, heat resisting plastic film as a base material, a pressure-sensitive adhesive layer 5 and a transparent release plastic film layer 6 built up successively in layers using a heat resisting material and provided with a cut line to peel off part of the release layer in contact with the flange 3.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The culture vessel of the present invention is mainly used for tissue culture of plants.

[0002]

2. Description of the Related Art Techniques for culturing plant buds, roots, leaves, petals and other tissue pieces in a sterile culture vessel and culturing the same individual in large quantities have been studied for various plants, and especially for flowers. Expensive things are becoming more common. Aseptic culture vessels used for conventional culture are mainly Erlenmeyer flasks,
A test tube and a transparent heat-resistant plastic container were used, and sterilization under pressure was carried out together with the medium to achieve sterility of the container. In addition, various sterile filters (filters that have the function of passing gas but not bacteria) for the purpose of maintaining appropriate oxygen concentration, carbon dioxide concentration and humidity inside without sterilization loss during culture. It was generally provided on the lid of the culture container.

[0003]

However, in such a conventional culture container, it is necessary to have transparency for accelerating photosynthesis and observation, and to endure heat sterilization under pressure. I had no choice but to use the material. For example, when glass is used, there are drawbacks such as a risk of damage during transportation, and the weight of the container is heavy.Also, even when plastic is used, the lid and the container body are fitted accurately, The methods were limited.

Further, culture vessels using these materials have the drawbacks of low gas permeability and low water vapor permeability and very slow growth of plants, and avoid the above-mentioned drawbacks while maintaining sterility. Therefore, measures are taken to attach various aseptic filters. However, even in this case, there are some drawbacks such as an increase in the price of the container itself and difficulty in dealing with various plants.

[0005]

Accordingly, as a result of earnest research to solve the above-mentioned problems, as a result, a container main body having a flange at an opening and a film lid, at least a portion of which faces the flange, is adhered. It is processed to make it removable, and in addition, a part of the lid is combined with a non-woven fabric that allows gas to pass but does not pass bacteria, or a lid that has a member having the function attached. The present invention has been completed based on the finding that it is possible to inexpensively provide a culture container that can cope with various plants by constructing the container with a material that can withstand heat sterilization under pressure.

The present invention will be described in more detail below with reference to the drawings. The culture container according to the present invention comprises a container body 1 (FIG. 1) and a sheet-like lid 2 (FIG. 2).
The characteristics of the container body must be transparent and capable of being sterilized by heating under pressure without impairing the hermeticity and shape. Materials satisfying the above conditions include glass, polypropylene resin, polycarbonate resin, methylpentene polymer (trade name TPX), etc., and can be appropriately selected.
The gas permeation amount and the water vapor permeation amount should be changed depending on the plant to be cultured, and it is preferable to match the material and thickness of the container body with the culture conditions. The size of the container is appropriately determined depending on the extent to which the plant to be cultured is allowed to grow.

A flange 3 (FIG. 1) is provided at the opening of the container body.
To provide. The flange is flat and its width is 5mm
The above is desirable.

The sheet-like lid 2 (FIG. 2) capable of sufficiently covering the container opening has a laminated structure and is composed of three layers. That is, layer 4 of the plastic film which is the base material
And a peeling layer 6 having releasability. This laminated sheet has a shape cut into a size sufficient to cover the opening of the container body. The peeling layer 6 is preferably made of a transparent plastic, and a notch 7 (FIGS. 2, 3, and 4) is formed in a portion of the opening of the container main body which is in contact with the flange surface in accordance with the shape, and a portion corresponding to the flange. It can be used as a lid by peeling off the release layer and adhesively sealing it to the container opening flange.

Next, the constituent materials of the lid member will be described. Although the basic characteristics required for the lid material are the same as those of the container body described above, it is premised that the lid material has good transparency and can endure the sterilization treatment under pressure. Therefore, a stretched film of polypropylene, polyester, polycarbonate or the like can be used as the plastic film of the substrate. The surface of the film on which the pressure-sensitive adhesive is laminated is preferably subjected to a primer coating or electrical treatment for enhancing the adhesion with the pressure-sensitive adhesive.

As the pressure-sensitive adhesive, it is necessary to maintain the hermeticity between the container body and the lid, and as the resin-based resin, acrylic resin, silicone-based resin, rubber-based resin or the like can be used, but heat resistance and stability From this point, it is preferable to use an acrylic or silicone resin adhesive.

As a release layer, in the case of a general tack seal, a paper called a release paper processed with a two-liquid reaction type silicone resin is often used, but in this case, it is transparent. Therefore, a polypropylene or polyester stretched film or the like processed with a silicone resin can be used.

In order to keep the oxygen concentration, carbon dioxide concentration, humidity, etc. in the container at an appropriate level, an opening is provided in a part of the lid sheet, and a non-woven fabric that allows gas and water vapor to pass therethrough without allowing bacteria to pass therethrough. The member 8 (FIG. 4) having the function can be attached. In the case of a non-woven fabric, the member to be adhered may be of a type such as polyester fiber, polyolefin fiber, or a mixed spinning thereof, and for other members, a membrane filter or the like can be used.

Regarding the sterilization after medium injection and the encapsulation of the sample, for example, the opening of the container body is covered with aluminum foil or the like, sterilized by autoclave, the medium is solidified, the plant tissue piece is inserted, and the lid is separately sterilized. Although it is possible to carry out hermetic packaging with a sheet, if the lid member is processed with a pressure sensitive adhesive having re-adhesiveness, the notch 7 as shown in FIG. Affixed to the flange part of the container body containing the medium in the peeled state, sterilized by autoclave, inserted the plant tissue piece, then peeled off the peeling layer 6b and crimped to the flange part to complete the sealing it can. This method can also simplify the work. Further, since both the container body and the lid can be made transparent, they are also suitable in terms of promoting photosynthesis and observing plants.

[0014]

According to the constitution of the present invention, it becomes possible to use a plastic film as a cover material, and it is possible to promote photosynthesis by improving transparency, sterilize the medium, and simplify the work of inserting and sealing plant tissue pieces. At the same time, the range of plastic materials that can be used for the container body is expanding. It is also possible to adjust the gas permeation amount and the water vapor permeation amount by attaching a sterile filter to a part of the lid material sheet.

[0015]

EXAMPLES Examples will be described in detail below. (Example 1) An unstretched polycarbonate (PC) sheet having a thickness of 1.5 mm was used as a container main body, and this was subjected to a conventional vacuum / compressed air forming method to obtain an opening inside dimension of 120 mm square and a bottom surface of 95 mm square and a depth. A 110 mm square container was molded. In addition, each corner portion was rounded, and the flange portion of the container was flat and 20 pieces were trimmed to have a width of 8 mm.

On the other hand, a biaxially oriented polypropylene film (hereinafter referred to as OPP) having a thickness of 60 μm is used as a cover material, and a film having corona discharge treatment on both surfaces is used as a surface substrate. The film thus formed was used as a release layer, and a silicone resin-based pressure sensitive adhesive was used to laminate the film on the surface substrate. Using this as a lid material sheet, as shown in FIG. 4, cut the outer periphery and cut into the release layer as shown in 7, and further opened a circular hole with a diameter of 10 mm in the center of the lid for attaching a sterile filter. .

Next, a membrane filter made of polypropylene resin was cut into a circle having a diameter of 25 mm, and it was positioned from the front side of the lid to the mounting hole of the sterile filter,
It was attached using the following two types of adhesives. Chlorinated polypropylene resin based adhesive (solvent type), 10 made. Two-component reactive polyurethane adhesive (solvent type), 10 created. The lid thus prepared was peeled off the peeling layer corresponding to 6a shown in FIG. 4 and then stuck to the flange of the container body containing the medium. The total quantity is 20 pieces.

The medium is Murashige-Skoog's medium,
One having a composition shown in Table 1 was prepared and used.

(Test 1) A total of 20 containers were placed in an autoclave containing these media, and a container equipped with a lid was used.
After sterilizing by heating under pressure at 30 ° C. for 30 minutes, the container was taken out and the appearance of the container was checked. The result is deformation of the container,
There was no peeling of the adhesive from the flange and the sterile filter, and the transparency of the container was good, so it was judged to be sufficiently practical as a culture container.

[0020]

[Table 1]

[0021]

EFFECTS OF THE INVENTION According to the present invention, it is possible to use a film having a pressure sensitive adhesive applied to the lid of a plastic culture container. Therefore, as described above, not only the promotion of photosynthesis due to the improvement of transparency and the ease of observation but also the manufacturing of containers can be performed by injection molding as well as sheet molding, and the molding itself. Since it is simple, it is easy to change the size of the container, and it is possible to inexpensively supply containers that are compatible with various plants. Therefore, it can be sufficiently used as a disposable container, further, by attaching a sterile filter to the lid material sheet, oxygen concentration in the container, carbon dioxide concentration, since it is possible to maintain a moderate humidity during the culture, for plant growth. The effect is that a suitable environment inside the container can be obtained.

[Brief description of drawings]

FIG. 1 is a perspective view showing an example of a culture container body of the present invention.

FIG. 2 is a cross-sectional view of a lid material sheet for a culture container of the present invention.

FIG. 3 is a bottom view of the lid of the culture container of the present invention.

FIG. 4 is a bottom view showing an example in which a sterile filter is attached to the lid of the culture container of the present invention.

[Explanation of symbols]

 1 Container Main Body 2 Lid Material Sheet 3 Flange 4 Plastic Film Layer 5 Adhesive Layer 6, 6a, 6b, 6c Release Layer 7 Notch 8 Sterile Filter

Claims (2)

[Claims] 1. A sterile culture container mainly used for tissue culture of plants, comprising a container body having a flange at an opening and a lid material sheet capable of sufficiently covering the container opening, A culture container characterized in that a portion which comes into contact with the flange surface is subjected to an adhesive treatment, and is constituted by a lid capable of being attached and detached. 2. A lid material sheet capable of sufficiently covering an opening of a container, wherein a part of the sheet is made of a non-woven fabric or a member having the function of allowing gases such as air and water vapor to pass but not bacteria. The culture container according to claim 1, wherein the adhered lid is used.