JPH0532031B2 - - Google Patents
Info
- Publication number
- JPH0532031B2 JPH0532031B2 JP59157794A JP15779484A JPH0532031B2 JP H0532031 B2 JPH0532031 B2 JP H0532031B2 JP 59157794 A JP59157794 A JP 59157794A JP 15779484 A JP15779484 A JP 15779484A JP H0532031 B2 JPH0532031 B2 JP H0532031B2
- Authority
- JP
- Japan
- Prior art keywords
- ifn
- medium
- cells
- producing
- lower alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000006992 Interferon-alpha Human genes 0.000 claims description 46
- 108010047761 Interferon-alpha Proteins 0.000 claims description 46
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 39
- 238000004519 manufacturing process Methods 0.000 claims description 15
- 230000006698 induction Effects 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- 239000003862 glucocorticoid Substances 0.000 claims description 4
- OHDXDNUPVVYWOV-UHFFFAOYSA-N n-methyl-1-(2-naphthalen-1-ylsulfanylphenyl)methanamine Chemical compound CNCC1=CC=CC=C1SC1=CC=CC2=CC=CC=C12 OHDXDNUPVVYWOV-UHFFFAOYSA-N 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 30
- 235000019441 ethanol Nutrition 0.000 description 24
- 239000002609 medium Substances 0.000 description 18
- 239000012980 RPMI-1640 medium Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 241000711408 Murine respirovirus Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 229960003957 dexamethasone Drugs 0.000 description 4
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000012888 bovine serum Substances 0.000 description 3
- 229960000890 hydrocortisone Drugs 0.000 description 3
- 208000011691 Burkitt lymphomas Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 229940037128 systemic glucocorticoids Drugs 0.000 description 2
- 208000023611 Burkitt leukaemia Diseases 0.000 description 1
- 239000012981 Hank's balanced salt solution Substances 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59157794A JPS6135794A (ja) | 1984-07-27 | 1984-07-27 | インタ−フエロン−αの製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59157794A JPS6135794A (ja) | 1984-07-27 | 1984-07-27 | インタ−フエロン−αの製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6135794A JPS6135794A (ja) | 1986-02-20 |
| JPH0532031B2 true JPH0532031B2 (cs) | 1993-05-14 |
Family
ID=15657428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59157794A Granted JPS6135794A (ja) | 1984-07-27 | 1984-07-27 | インタ−フエロン−αの製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6135794A (cs) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2573258B2 (ja) * | 1987-10-27 | 1997-01-22 | 松下電工株式会社 | 焦電素子センサー装置の構造 |
-
1984
- 1984-07-27 JP JP59157794A patent/JPS6135794A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6135794A (ja) | 1986-02-20 |
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