JPH0529066B2 - - Google Patents
Info
- Publication number
- JPH0529066B2 JPH0529066B2 JP61223682A JP22368286A JPH0529066B2 JP H0529066 B2 JPH0529066 B2 JP H0529066B2 JP 61223682 A JP61223682 A JP 61223682A JP 22368286 A JP22368286 A JP 22368286A JP H0529066 B2 JPH0529066 B2 JP H0529066B2
- Authority
- JP
- Japan
- Prior art keywords
- immobilized
- antigens
- membrane
- electrophoresis
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000427 antigen Substances 0.000 claims description 20
- 102000036639 antigens Human genes 0.000 claims description 20
- 108091007433 antigens Proteins 0.000 claims description 20
- 239000012528 membrane Substances 0.000 claims description 20
- 238000005259 measurement Methods 0.000 claims description 16
- 238000001962 electrophoresis Methods 0.000 claims description 10
- 239000000969 carrier Substances 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000003018 immunoassay Methods 0.000 claims description 7
- 230000003100 immobilizing effect Effects 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 15
- 239000003792 electrolyte Substances 0.000 description 13
- 238000002347 injection Methods 0.000 description 13
- 239000007924 injection Substances 0.000 description 13
- 206010028980 Neoplasm Diseases 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 239000011521 glass Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 201000011510 cancer Diseases 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000008151 electrolyte solution Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 3
- 102000012406 Carcinoembryonic Antigen Human genes 0.000 description 3
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 3
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 229910000077 silane Inorganic materials 0.000 description 3
- 239000000439 tumor marker Substances 0.000 description 3
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 2
- 229920000178 Acrylic resin Polymers 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- PKZCRWFNSBIBEW-UHFFFAOYSA-N 2-n,2-n,2-trimethylpropane-1,2-diamine Chemical compound CN(C)C(C)(C)CN PKZCRWFNSBIBEW-UHFFFAOYSA-N 0.000 description 1
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- XDLMVUHYZWKMMD-UHFFFAOYSA-N 3-trimethoxysilylpropyl 2-methylprop-2-enoate Chemical compound CO[Si](OC)(OC)CCCOC(=O)C(C)=C XDLMVUHYZWKMMD-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61223682A JPS6379071A (ja) | 1986-09-24 | 1986-09-24 | 多項目イムノアツセイ |
| US07/031,665 US5057438A (en) | 1986-09-24 | 1987-03-30 | Electrophoretic antigen-antibody determination with laminate of multiple membranes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61223682A JPS6379071A (ja) | 1986-09-24 | 1986-09-24 | 多項目イムノアツセイ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6379071A JPS6379071A (ja) | 1988-04-09 |
| JPH0529066B2 true JPH0529066B2 (pt) | 1993-04-28 |
Family
ID=16801994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61223682A Granted JPS6379071A (ja) | 1986-09-24 | 1986-09-24 | 多項目イムノアツセイ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6379071A (pt) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340452A (en) * | 1991-02-01 | 1994-08-23 | Beckman Instruments, Inc. | On-column preconcentration of samples in capillary electrophoresis |
| CA2586515A1 (en) * | 2003-11-05 | 2005-05-26 | Exact Sciences Corporation | Repetitive reversed-field affinity electrophoresis and uses therefor |
| CN107128864B (zh) * | 2017-03-21 | 2019-02-05 | 上海万生昊天生物技术有限公司 | 用于梯度凝胶胶片连续制作的全自动控制灌胶系统及方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55132956A (en) * | 1979-01-31 | 1980-10-16 | Technicon Instr | Reaction method of and apparatus for composition of fluid sample and preeconcentrating apparatus |
| JPS6057257A (ja) * | 1983-09-09 | 1985-04-03 | Hitachi Ltd | イムノアツセイ法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5258086U (pt) * | 1975-10-23 | 1977-04-27 |
-
1986
- 1986-09-24 JP JP61223682A patent/JPS6379071A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55132956A (en) * | 1979-01-31 | 1980-10-16 | Technicon Instr | Reaction method of and apparatus for composition of fluid sample and preeconcentrating apparatus |
| JPS6057257A (ja) * | 1983-09-09 | 1985-04-03 | Hitachi Ltd | イムノアツセイ法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6379071A (ja) | 1988-04-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |