JPH05268883A - Feed for pig and method for rearing pig using the same - Google Patents

Abstract

PURPOSE:To obtain a feed for pigs, comprising a casein hydrolyzate consisting essentially of a casein phosphopeptide and useful for promoting the absorption of minerals such as Ca or iron and improving the blood properties and milk quality of a dam sow, blood properties and ossification degree of a piglet. CONSTITUTION:The objective feed comprises a casein hydrolysate consisting essentially of a casein phosphopeptide(CPP) in an amount of preferably 0.002-0.2 pt.wt. based on 100 pts.wt. feed for pigs. The casein hydrolyzate consisting essentially of the CPP is preferably obtained by a method for dissolving a raw material casein such as acid casein or sodium caseinate in water, then using trypsin in an amount corresponding to 0.001-2wt.% expressed in terms of crystal trypsin based on the weight of the substrate casein and carrying out reaction at 2-30% casein concentration, 20-50 deg.C temperature and pH6-9 for 5min to 100hr.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to casein phosphopeptides (hereinafter abbreviated as CPP).
The casein hydrolyzate containing as the main component promotes the absorption of minerals such as calcium and iron, thereby improving the mineral content of blood and milk of pigs, and as a result, the bone formation and body weight of seed pigs and born piglets. And a method for raising pigs.

[0002]

2. Description of the Related Art In recent years, in the field of production of edible animals, technological progress such as productivity improvement has been remarkable due to improvement of breeding environment, enlargement of scale, breed improvement and feeding system.

In order to maintain animal life, it is necessary for the feed to be properly blended with various nutrients.
Especially in piglets that are required to grow rapidly, the utilization of minerals, especially calcium, may be poor, and bone growth cannot keep up with weight gain, causing leg weakness and claw cracking. On the other hand, even in pigs, leg weakness due to lack of mineral absorption during breeding and insufficient mineral supplementation from the pregnant pig to the fetus during pregnancy.

The nutritional status of seed pigs and piglets appears in weight gain, skeleton, and blood properties, and minerals are the nutrients that form the basis thereof. Therefore, calcium, phosphorus, various vitamins and minerals are sufficiently mixed in ordinary feeds for seedlings and piglets.

The property of CPP has been clarified as a calcium absorption promoting factor (Hiro Naito, Chemistry and Biology,
18 , 551-558, 1980, YS Lee, T. Noguchi and H. Ni
to, Brit. J. Nutr., 43 , 457-467, 1980, USP4,816,3
98).

[0006]

However, satisfactory results have not been obtained even if the amounts of these components are increased. Therefore, it has been desired to develop a substance and a method for promoting the absorption rate of minerals such as calcium and iron to improve the milk quality of seed pigs and the blood properties of piglets. It is known to administer CPP to humans, but not to use it in livestock feed.

[0007]

Means for Solving the Problems As a result of intensive studies to achieve such an object, the present inventor has added the casein hydrolyzate containing CPP as a main component to a feed for seed pigs to feed it. It was found that absorption of minerals such as calcium and iron is promoted, and that blood characteristics and milk quality of seed pigs are effective in improving blood characteristics and ossification degree of piglets, and gaining weight.
The present invention has been completed based on this finding.

That is, the present invention relates to a feed for pigs and a method for raising pigs, characterized by containing a casein decomposition product containing CPP as a main component.

It is known that there are two typical types of CPP. That is, those derived from α-casein (abbreviated as α-CPP) and those derived from β-casein (β-
CPP) and their structure and characteristic values are as shown in Table 1.

[0010]

[Table 1]

Abbreviations for amino acid residues in the above structure are IUPA
It is due to C, and all are L-forms except glycine. Ser ^P represents phosphoserine.

Α-CPP derived from α-casein is a peptide chain portion from Asp ⁴³ to Lys ⁷⁹ , β derived from β-casein.
-CPP is said to be a peptide chain portion from the N terminus to Arg ^25, but the casein degradation product added to the feed of the present invention contains these α and β-CPP and other peptides. The CPP content in this casein degradation product is 6
It is about 18 to 18% by weight, preferably about 9 to 15% by weight.
CPP content, Fe ^{3 +} _- affinity chromatography to isolate the non-phosphorus-containing peptide by adsorbing phosphoserine portion of CPP, then the phosphorus-containing peptide by reverse-phase high performance liquid chromatography eluting the adsorbed portion Quantitatively required. Therefore, among various degradation products of casein, a phosphopeptide, that is, a peptide containing 1 to 7 phosphoserines is quantified as CPP.

In the casein-decomposed product containing CPP as the main component used in the present invention, for example, the raw material casein obtained by allowing trypsin to act on casein is the best casein such as acid casein, casein sodium, and casein calcium.
Unpurified products such as milk and skim milk can also be used as raw materials.

This raw material casein is dissolved in water, and trypsin or an enzyme preparation containing trypsin is added. As the trypsin or the enzyme preparation containing the same used in the present invention, for example, a commercially available enzyme derived from the pancreas (pancreatin) is sufficient, but a crystal grade one is preferable from the viewpoint of yield and the like. CPP is produced by reacting the raw material casein with the above enzyme. The amount of enzyme used is 0.001% to 2% as crystalline trypsin relative to the weight of substrate casein.
A considerable amount is suitable. Industrial reaction conditions at this time, the casein concentration during the reaction is 30% or less, preferably 2 to 30%,
The reaction pH is 6.0 to 9.0, the reaction temperature is 15 to 60 ° C, preferably 20.
The reaction proceeds sufficiently at -50 ° C and a reaction time of 5 minutes to 100 hours to produce CPP.

The casein hydrolyzate thus obtained is uniformly mixed with 0.01 to 1 part by weight, preferably 0.02 to 0.2 part by weight, per 100 parts by weight of a normal pig feed. This pig feed may be fed to pigs at all times, but it is preferable to feed pigs from the 50th day of gestation until weaning, when the nutritional requirements of the embryo are high from the viewpoint of economy and effect. It is also effective for piglets about 180 days after weaning.

[0016]

[Function] CPP contains phosphoserine (-Ser) in its molecule.
^P- ) is possessed, and this phosphoserine binds to the mineral in the digestive tract to solubilize the mineral itself, and as a result, absorption of the mineral from the intestinal tract is promoted.

[0017]

【Example】

Example 1 0.04% of a casein degradation product (Meiji CPP-I, a product of Meiji Seika Co., Ltd.) containing 12% by weight of CPP was added to a commercially available feed for seed pigs.
The animals were divided into two groups, a test group (6 animals) administered with the mixture by weight% and a control group (4 animals) not administered with the casein degradation product, and feeding was started from the 50th day of pregnancy.

The results of blood and milk of seed pigs are shown in Tables 2 and 3. Blood is hemoglobin amount (hereinafter, Hb), blood cell volume ratio (hereinafter, Ht), serum iron content (hereinafter, Fe), serum calcium (hereinafter, Ca), serum magnesium (hereinafter, Mg),
Regarding serum inorganic phosphorus (hereinafter, IP) and serum total protein amount (hereinafter, TP), milk has iron (hereinafter, M · Fe), calcium (hereinafter, M · Ca), magnesium (hereinafter, M · Fe).
Mg), inorganic phosphorus (hereinafter, M.IP), and total protein amount (hereinafter, M.TP) were measured.

[0019]

[Table 2]

[0020]

[Table 3]

As shown in Table 2, the blood of pregnant pigs had significantly higher Fe and Mg in the test section on the day of delivery than in the control group.

As shown in Table 3, in milk, the Fe, Ca, Mg, IP, and TP in the test group on the day of delivery were significantly higher on the 21st day after delivery than in the control group.

Example 2 63 pigs born from the mother pigs in the test group and 40 pigs born from the mother pigs in the control group bred by the method described in Example 1 were fed 23 days after birth. Live with the mother pig until weaning,
Breed only with breast milk.

Table 4 shows the blood properties and body weight of the offspring at 7, 21, and 35 days after birth, and Table 5 shows the condition of the bones of the lower leg at weaning (21 days after birth).

[0025]

[Table 4]

[0026]

[Table 5]

As shown in Table 4, Ht, Fe, and
In Mg, IP, TP and body weight, 21 days after delivery, for Hb, Ht, Mg, IP and TP, and delivery 35
After days, the Fe, IP and body weight of the offspring produced from the sows in the test group were significantly higher than those of the control group.

As shown in Table 5, the degree of ossification was higher in the test group than in the control group.

Example 3 A casein degradation product (Meiji CPP-I, Meiji Seika Co., Ltd.) containing 12% by weight of CPP in commercially available feed from the 15th day of life
Test group (63%)
2) of control group (40 heads) not administered with casein degradation product
The animals were divided into groups and each of them started feeding. From the 22nd day after birth, the feed was changed to a commercially available feed for fattening and the test was continued in the same test group.

Tables 6 and 7 show the blood properties of piglets and the results of measuring the degree of ossification by X-ray analysis.

Blood contains hemoglobin amount (hereinafter, Hb), blood cell volume ratio (hereinafter, Ht), serum iron content (hereinafter, Fe), serum calcium (hereinafter, Ca), serum magnesium (hereinafter, M).
g), serum inorganic phosphorus (IP) and serum total protein (TP), at the time of weaning (21 after birth) for bone.
On the day 1), the lower legs of all piglets were radiographed, and the shadow images were measured with a transmission densitometer to infer the bone ossification state.

[0032]

[Table 6]

[0033]

[Table 7]

As shown in Table 6, the blood properties of piglets in TP were significantly higher in the test group than in the control group.

As shown in Table 7, the ossification degree of the piglets was higher in the test group than in the control group in terms of the ossification degree in the central fibula and the central talus.

[0036]

EFFECTS OF THE INVENTION By feeding the pig feed of the present invention, the absorption of minerals such as calcium and iron during pregnancy is promoted in the pig, and the blood characteristics and milk quality of the pig are improved.
In addition, it improves blood characteristics and ossification degree of offspring and leads to weight gain. In addition, since it also leads to improvement of blood characteristics and ossification degree for piglets, productivity and feed efficiency are improved, which is a great advantage for pig farmers.

Claims (3)

[Claims] 1. A pig feed comprising a casein degradation product containing casein phosphopeptide as a main component. 2. The pig feed according to claim 1, wherein the casein hydrolyzate containing casein phosphopeptide as a main component is 0.01 to 1 part by weight per 100 parts by weight of the feed. 3. A method for breeding pigs, characterized in that 0.01 to 1 part by weight of a casein degradation product containing casein phosphopeptide as a main component is used per 100 parts by weight of the feed.