JP2995440B2 - Feed for young animals - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a feed for young animals which contains calcium casein hydrolyzate which is an excellent source of protein and nitrogen and also serves as a mineral absorption promoter. In detail, a casein calcium degradation product mainly composed of a peptide with a molecular weight of 1,000 to 5,000, which is obtained by treating casein calcium with a protease of Aspergillus genus, which is excellent as a protein and nitrogen source and has a mineral absorption promoting action The present invention relates to a feed for a young animal which contains and improves the growth and bone formation of a young animal such as a pig or a cow.

[0002]

2. Description of the Related Art Feed for young animals contains a protein source, an energy source, vitamins, minerals such as calcium, and the like. As a protein source, soybean meal, concentrated soybean protein, fish meal, skim milk and the like are blended, and as a calcium source, calcium carbonate and the like are blended. In recent years, the rearing space per animal has been reduced due to the increase and concentration of livestock breeding, and as a result, the activity of livestock has been reduced, and the growth of livestock, especially the growth of bones in young animals, has become an environmental issue. Deterioration of conditions has become a problem. To improve such bone growth, it is necessary to provide a large amount of calcium to measure its absorption. For this purpose, calcium and vitamins necessary for bone growth are incorporated in commercially available feeds for young animals. However, even if the amounts of these components are increased, there is a limit in promoting bone growth, and no satisfactory method has been developed yet.

[0003]

DISCLOSURE OF THE INVENTION The present invention relates to a plant containing a calcium casein hydrolyzate, which has an effect of improving the growth of a young animal at the time of breeding and efficiently promoting the absorption of minerals, especially calcium, which tend to be insufficient. It provides a feed for aged animals.

[0004]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies for the purpose of developing a feed which improves the growth of young animals and promotes the absorption of minerals such as calcium. The inventors have found that a calcium casein hydrolyzate having a molecular weight of 1,000 to 5,000 as a main component, which is obtained by treatment with a proteolytic enzyme derived from a genus Aspergillus, achieves the above object, and has completed the present invention. That is, the present invention improves the growth of young animals, and is obtained by treating casein calcium hydrolyzate having an action of promoting the efficient absorption of minerals such as calcium, that is, treating casein calcium with a proteolytic enzyme derived from Aspergillus. The present invention relates to a feed for young animals containing calcium casein hydrolyzate having a molecular weight of 1,000 to 5,000 as a main component.

The calcium caseinate hydrolyzate of the present invention is obtained by suspending and dispersing calcium caseinate as a raw material in water in the range of 2 to 40% by weight, preferably 10 to 25% by weight, to obtain a protease of Aspergillus genus. It is obtained by adding and reacting for 5 to 48 hours, preferably 10 to 24 hours while stirring at a temperature range of 25 to 70 ° C, preferably 40 to 60 ° C. The protease used in the present invention is an enzyme derived from a genus Aspergillus, and among them, a neutral protease is preferable. An enzyme other than the neutral protease can be used, but pH adjustment is required at the time of the enzyme treatment, and when the enzyme is neutralized after the treatment, a salt is generated, so that there is a problem that the salt must be removed. Commercially available proteolytic enzymes of the genus Aspergillus include, for example, "Protease P Amano", "Protease A Amano" (manufactured by Amano Pharmaceutical Co., Ltd.) and "Morcin" (manufactured by Fujisawa Pharmaceutical Co., Ltd.) ,
"Orientase ON" (manufactured by Ueda Chemical Industry Co., Ltd.) and the like. These enzymes may be used alone or in combination of two or more.

[0006] The protease used in the present invention is 0.1 to 10% by weight, preferably 0.1 to 10% by weight, based on calcium caseinate.
It is used by adding 0.5 to 5% by weight. Protease is 0.
If it is less than 1%, calcium caseinate is hardly decomposed, and the yield of the obtained peptide is low. When the amount of the protease is 10% by weight or more, the decomposition reaction of calcium caseinate occurs without any problem, but there is a problem that it is economically expensive. The treatment temperature with the protease of the present invention is 25 to 70.
C., preferably a temperature range of 40 to 60.degree. C. If the treatment temperature is 25.degree. Also processing temperature
Above 70 ° C, the enzyme is severely deactivated, making it difficult to control the decomposition reaction. The calcium caseinate used in the present invention is suspended and dispersed in water in the range of 2 to 40% by weight, preferably 10 to 25% by weight, and is decomposed with an enzyme. , Low yields result in lower yields. If the content is more than 40% by weight, there is a problem that the viscosity of the solution increases and stirring becomes difficult, and it becomes difficult to perform suspension and dispersion uniformly.

The calcium caseinate hydrolyzate of the aqueous solution obtained by the method of the present invention can be commercialized in the form of an aqueous solution as it is, or powdered by a method such as spray drying or freeze drying. The calcium caseinate hydrolyzate obtained by the method of the present invention has a molecular weight of 10
It has a casein calcium peptide of from 00 to 5000 as a main component. The calcium casein hydrolyzate of the present invention may be added to a feed for young animals in an amount of usually 0.01 to 40% by weight, preferably 0.1 to 20% by weight as a protein source and a mineral absorption promoting substance such as calcium, or a protein source. Can be replaced by If it is 0.01% by weight or less, the effect of the present invention cannot be expected so much. On the other hand, if the content is 40% by weight or more, the balance of other essential growth components is lost, which is not good for the growth of young animals. Other feed components may be the same as known feeds for young animals, and protein sources other than the calcium caseinate hydrolyzate of the present invention may include soybean meal, concentrated soybean protein, fish meal, skim milk and the like. As a calcium source, calcium carbonate or the like can be blended.
Next, the present invention will be described in detail with reference to examples.

[0008]

【Example】

Example 1 500 g of calcium caseinate powder (from New Zealand) having a calcium content of 1.3% was suspended in 5 liters of water, and 10 g of a neutral protease originating from a genus Aspergillus [trade name: Protease P "Amano" manufactured by Amano Pharmaceutical Co., Ltd.] Was added and reacted at 45 ° C. for 20 hours. Next, at 80 ℃, 30
The enzyme was inactivated by a heat treatment for minutes, followed by filtration. The filtrate was spray-dried to obtain 450 g of calcium casein hydrolyzate. The obtained calcium casein hydrolyzate was subjected to gel filtration chromatography using high performance liquid chromatography (manufactured by Nippon Waters Limited). Using Superose 12 (manufactured by Pharmacia) as a column for analysis, a phosphate buffer (pH = 7)
Flow at a flow rate of 1 / min and detection at 220 nm. As a result of the measurement, the molecular weight of the obtained casein calcium decomposition product was 1000 to
The 5000 fraction was 75.2%.

Test Example 1 Amino acid absorption test in rat small intestine The following experiment was conducted in order to examine the effect of the product of the present invention of Example 1 on amino acid absorption from the intestinal tract. Weight about 200
g of a male Wistar rat, a catheter for injecting a sample into the duodenum and a catheter for collecting blood from the portal vein were inserted into the portal vein. After the operation, the animals were fed solid food and water freely and kept for 2 days. The absorption experiment was performed on the third day after an overnight fast, and 1 ml of the sample solution was injected into the duodenum.
Blood was collected from the portal vein at 0, 60 and 120 minutes. In addition, before the sample administration, blood was collected 0 minutes later. Amino groups in the plasma of the collected blood were measured by the trinitrobenzene sulfonic acid method, and individual free amino acids were analyzed by Hitachi 8
Quantification was performed using a 500-type amino acid automatic analyzer. The administration samples were divided into the following three groups, each of which was administered in five cases.

Group A: product of the present invention (20% aqueous solution) Group B: 20% free amino acid mixture (amino acid mixture having the same composition as the product of the present invention) Group C: 20% calcium caseinate aqueous solution Only done. The results of this experiment are shown below.

[0011]

[Table 1]

[0012]

[Table 2]

[0013]

[Table 3]

As a result of quantifying the total amino groups in the plasma of blood collected from the portal vein, as shown in Table 1, the concentration of amino groups in Group A was clearly higher than those in Groups B and C. (P <
0.05). Furthermore, as a result of quantifying the total free amino acid concentration in the same manner, as shown in Table 2, the total free amino acid concentration of Group A was
It was higher than the group. Further, as a result of quantifying the total essential amino acid concentration, as shown in Table 3, the total essential amino acid concentration of group A was higher than that of group B (P <0.05). From the above, it can be concluded that the calcium caseinate hydrolyzate of the present invention is superior in absorption of amino acids from the intestinal tract as compared with other materials, and is superior in comparison of the absorption amount of essential amino acids.

Test Example 2. Calcium absorption test by intestinal loop method The following experiment was conducted in order to examine the effect of the product of the present invention of Example 1 on the absorption of calcium from the intestinal tract. Weight about 2
After fasting a 00 g male Wistar rat overnight, a ligated intestinal loop was formed in the upper part of the small intestine under anesthesia. Sample is 40
0.2 ml of mM phosphate buffer (pH 7.4) and then the sample solution were injected into the 0.2 ml loop using a syringe. After the injection, the small intestine was returned to the intraperitoneal cavity, left under anesthesia for 2 hours, and then the liquid in the loop was taken out. The solution in the loop was collected by washing with a 0.01 N hydrochloric acid solution. In another rat, the solution in the loop was immediately collected after the sample injection, and this was set to 0 hour. The total amount of calcium remaining in the solution in the loop at 0 and 2 hours after collection was measured using a calcium measurement kit [Calcium C-Test Wako; manufactured by Wako Pure Chemical Industries, Ltd.] to calculate the calcium absorption rate (%). did. The administration sample was divided into the following four groups, and the total calcium concentration in the sample was adjusted to 28.5 mM (calculated as calcium chloride). The number of cases was four each.

Group D: 28.5 mM calcium chloride solution Group E: invention product (10% aqueous solution) Group F: invention product (2.58%) + 21.2 mM calcium chloride solution Group G: 10% calcium casein solution Results of this experiment Is shown below.

[0016]

[Table 4]

From the experimental results shown in Table 4, the calcium absorption rate of group E was significantly higher than that of group D or G containing the same amount of calcium (P <0.05). Also, in the case of group F in which calcium chloride was added to the product of the present invention, the calcium absorption was almost the same as in the case of group E. From the above, it can be determined that the calcium casein hydrolyzate of the present invention is superior in calcium absorption as compared with other materials, and has an effect of increasing the absorption of inorganic calcium.

Test Example 3 Growth test of piglets The feed containing 1% by weight, 3% by weight, and 5% by weight of the calcium casein hydrolyzate obtained in Example 1 was added to a commercially available feed, and the same feed as a comparative example was used in place of calcium casein hydrolyzate. Total of 6 feeds mixed with the same amount of casein as above
Different feeds were prepared. The test was carried out on piglets 3 weeks of age and a total of 7 days of the above feed and no additive feed as shown below.
In the group, a feeding test was carried out for 15 days by freely feeding each feed. The number of cases was 13 each.

Group H: Additive-free feed Group I: Addition of 1% by weight of the product of the present invention Group J: Addition of 3% by weight of the product of the invention K Group: Addition of 5% by weight of the product of the invention L Group: Addition of 1% by weight of casein Group M: Casein 3% by weight added N group: Casein 5% by weight added Feeding results are shown below.

[0020]

[Table 5]

From the experimental results in Table 5, it was found that the group to which the calcium casein hydrolyzate was added significantly increased body weight as compared with the other groups. From the above, it can be determined that the calcium casein hydrolyzate of the present invention has an effect of improving the growth of piglets as compared with other materials.

[0022]

The molecular weight of 10 obtained by the method of the present invention.
Calcium casein hydrolyzate containing peptides of 00 to 5,000 as a main component is excellent as a protein / nitrogen source, has excellent amino acid absorption, and has a mineral absorption promoting action, and is obtained by blending the calcium casein hydrolyzate of the present invention with minerals. Feed is easy to absorb protein and minerals,
It can improve the malabsorption of minerals such as calcium and improve the growth of young animals. Further, the present invention can be applied not only to young animals but also to feed for grown animals.

Claims (1)

(57) [Claims] 1. A molecular weight of 100 obtained by treating calcium caseinate with a proteolytic enzyme of Aspergillus genus.
A growth promoting composition for a young animal, comprising a calcium casein hydrolyzate containing 0 to 5000 peptides.